Synergistic strategies for glioblastoma treatment: CRISPR-based multigene editing combined with immune checkpoint blockade by Xiaolin Liu, Xiao Liu, Xiaonan Luo, Maorong Zhu, Nannan Liu, Juan Li, Qi Zhang, Cheng Zou, Yuxin Wu, Zhengcong Cao, Shuangxin Ma, Weizhong Wang, Guangzhao Yang, Jintao Gu, Wei Liu, Meng Li, Anan Yin, Yalong He, Wei Lin

“…Furthermore, the EVs contained Cas9 protein and sgRNA for precise and minimally invasive gene therapy, which addressing the key barriers associated with in vivo CRISPR‒Cas9 gene editing treatment. …”
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Novel role of microphthalmia-associated transcription factor in modulating the differentiation and immunosuppressive functions of myeloid-derived suppressor cells by Yoon Kyung Jeon, Aram Lee, Haesun Park, Soyoung Lim, Jihyun Lim, Jaemoon Koh, Young Yang, Myeong-Sok Lee, Jong-Seok Lim

“…MITF overexpression in myeloid cells increased the expression of MDSC activity markers and effectively inhibited T-cell proliferation compared with those of control MDSCs, whereas shRNA-mediated knockdown of MITF in myeloid cells altered the immunosuppressive function of MDSCs. …”
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Suppressing GDF15 enhances the chemotherapeutic effect of 5 FU on MSI-H CRC by regulating the ferroptosis pathway SLC7A11/GSH/GPX4 by Zhi min Huang Fu, Ming Xiao, Hailun Xie, Shuxian Zhang, Tang Yi, Qingshu Li, Ming Li, Yalan Wang

“…Suppressing GDF15 by siRNA resulted in a substantial decrease in cell viability and proliferation. …”
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Sorafenib enhanced the function of myeloid-derived suppressor cells in hepatocellular carcinoma by facilitating PPARα-mediated fatty acid oxidation by Chunxiao Li, Liting Xiong, Yuhan Yang, Ping Jiang, Junjie Wang, Mengyuan Li, Shuhua Wei, Suqing Tian, Yuexuan Wang, Mi Zhang, Jie Tang

“…These included subcutaneous tumor models, cell viability assays, flow cytometry, immunohistochemistry, and RNA sequencing. MDSCs were analyzed for chemotaxis, immunosuppressive functions, fatty acid oxidation (FAO), and PPARα expression. …”
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Low-Concentration Oxygen/Ozone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMP/cGMP-NF-κB/p65 Signaling in Chronic Radiculitis Rats by Junnan Wang, Mingyi Wu, Xiaowen Lin, Yun Li, Zhijian Fu

“…Moreover, intrathecal administration of 20 μg/mL oxygen/ozone reversed the increased levels of spinal PDE2A and NF-κB/p65 mRNA and protein expressions in rats with chronic radiculitis. …”
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Morphological and functional analysis of colorectal cancer cell lines in 2D and 3D culture models by Seiichi Shinji, Yutaro Ogawa, Takeshi Yamada, Akihisa Matsuda, Kay Uehara, Yasuyuki Yokoyama, Goro Takahashi, Takuma Iwai, Toshimitsu Miyasaka, Shintaro Kanaka, Koki Hayashi, Yuuki Shichi, Masakazu Fujiwara, Kimimasa Takahashi, Tomio Arai, Toshiyuki Ishiwata, Hiroshi Yoshida

“…In 3D cultures using low-attachment plates, mesenchymal cells from 2D cultures showed reduced vimentin mRNA levels. Morphologically, the five CRAC cell lines appeared similarly shaped in 2D culture but formed different structures in 3D culture. …”
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Identification of the RHD novel allele c. 801+2T>G and study of its effect on RhD phenotype in vitro by JIA Shuangshuang, WEN Jizhi, WEI Ling, ZHANG Runqing, LUO Guangping, JI Yanli

“…By using an in vitro micro gene splicing system, the mRNA splicing results were detected and analyzed using agarose and capillary electrophoresis to predict their impact on RhD phenotype. …”
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Hirudo extract ameliorates proliferative vitreoretinopathy by promoting autophagy and attenuating the THBS2/PI3K/Akt pathway by Hui Huang, Qun Huang, Jing Wang, Wanjie Wang, Yanlin Zheng

“…Autophagy was observed via transmission electron microscopy. The mRNA expression of MMP9, N-cadherin, vimentin, THBS2, PI3K, and Akt was measured via RT‒qPCR. …”
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Resveratrol Alleviates NEFA-Induced Oxidative Damage in Bovine Mammary Epithelial Cells by Restoring Mitochondrial Function by Longwei Sun, Junpeng Huang, Xiangyang Dou, Zhenyu Dong, Yuan Li, Shujing Tan, Ran Yu, Chengmin Li, Weiguo Zhao

“…In addition, in vivo studies in a mouse model of oxidative damage induced by high-fat diet (HFD) demonstrated that RES alleviated oxidative damage (decreased MDA content) and mitochondrial dysfunction (decreased expression of Drp1 and Fis1 and increased levels of Mfn2, Cyt C mRNA and ATP production) in mammary gland tissue. …”
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Unveiling the UNESCO Biosphere Reserve of the Berlengas Archipelago in Portugal as a Hotspot of Fish Species Using eDNA Metabarcoding and the Collaboration of Fishing Crews by Marco Simões, Cátia Costa, Maria da Luz Calado, Nuno Vasco-Rodrigues, Maria Jorge Campos, Sérgio Miguel Leandro, Agostinho Antunes

“…This study aims to evaluate the spatiotemporal diversity of fish using 12S rRNA gene eDNA metabarcoding sequencing in the Berlengas archipelago and compare two seawater eDNA sampling sources: samples collected by fishermen during their activities and those collected by our research team. …”
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Identification of <i>ZmSNAC06</i>, a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in <i>Arabidopsis</i> by Fei Wang, Yong Chen, Ruisi Yang, Ping Luo, Houwen Wang, Runze Zhang, Wenzhe Li, Ke Yang, Xinlong Xu, Zhuanfang Hao, Xinhai Li

“…Here, we identified a maize NAC transcription factor, <i>ZmSNAC06</i>, between drought-tolerant and drought-sensitive inbred lines through RNA-seq analysis and characterized its function in <i>Arabidopsis</i>. …”
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Genes related to neural tube defects and glioblastoma by Rui Cao, Yurong Liu, Kaixin Wei, Ning Jin, Yuxiang Liang, Ruifang Ao, Weiwei Pan, Xiang Wang, Xiuwei Wang, Li Zhang, Jun Xie

“…Our results indicated that two genes at mRNA and protein levels have been well verified in both NTDs mouse and GBM human samples, namely, Poli and Fgf1. …”
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ET-1通过上调CXCR4表达促进鼻咽癌低转移 细胞株6-10B的迁徙

“…【目的】 探讨内皮素-1(ET-1)上调CXCR4表达促进鼻咽癌低转移细胞株6-10B迁徙的机制【方法】 加入不同浓度的ET-1以及同时加入SDF-1α处理6-10B细胞,趋化实验检测ET-1对6-10B细胞迁徙能力的影响分别采用Real-time PCR以及Western Blot方法检测ET-1上调后CXCR4在基因以及蛋白水平的变化Western Blot方法检测用特异性的ETAR抑制剂或者PI3K/AKT/mTOR或MARK1/ERK1/2抑制剂预处理6-10细胞后,ET-1对CXCR4表达水平的变化;并探讨ET-1处理后,PI3K/AKT/mTOR以及MARK1/ERK1/2信号通路中蛋白磷酸化水平的改变【结果】 趋化实验显示6-10B细胞丧失对SDF-1α的趋化能力,但不同浓度的ET-1刺激后6-10B细胞对SDF-1α的迁徙能力明显增强(P < 0.05),10 nmol/L ET-1的作用最明显Real-time PCR以及Western Blot结果显示,不同浓度的ET-1处理6-10B细胞后,ET-1在基因以及蛋白的水平可以呈剂量依赖性及时间依赖性地上调CXCR4的表达用特异性的内皮素受体A(ETAR)抑制剂或者PI3K/AKT/mTOR或MARK1/ERK1/2通路的抑制剂,能够明显抑制ET-1对CXCR4表达的上调Western blot检测显示ET-1能激活PI3K/AKT/mTOR及MAPK1/ERK1/2信号通路【结论】 ET-1能上调6-10B细胞功能性CXCR4的mRNA和蛋白表达,并能明显增强6-10B细胞对SDF-1α的迁徙能力CXCR4的上调主要是通过ETAR介导,激活PI3K/AKT/mTOR和MAPK1/ERK1/2信号通路…”
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甲状腺功能亢进激活心肌细胞β-catenin/FoxO1诱导大鼠心室重构的作用 by 袁勋, 班莉, 田松麟, 朱秋连, 张贵平, 覃媛, 潘丽, 侯宁

“…利用原代培养的乳鼠心肌细胞，使用甲状腺激素（T3 20 nmol/L）处理心肌细胞24 h构建体外甲亢诱导心肌细胞肥厚模型，利用β-cateninsiRNA（30 nmol/L）转染心肌细胞敲低β-catenin，Western-blot和免疫荧光检测抑制β-catenin对甲亢诱导乳鼠心肌细胞肥厚的影响。…”
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Protective effect of brain and muscle arnt-like protein-1 against ethanol-induced ferroptosis by activating Nrf2 in mice liver and HepG2 cells by Yanan Zhao, Ranran Zhang, Ziheng Chen, Ziyi Wang, Shuang Guan, Jing Lu

“…Further study indicated that the alcohol could activate the protein expression of brain and muscle arnt-like protein-1 (BMAL1) which exerts a protective effect against ferroptosis through promoting nuclear factor erythroid 2-related factor 2 (Nrf2) translocation into nuclear and subsequently stimulating its downstream proteins FTH, FPN, glutathione peroxidase 4 activity (GPX4), HO-1, SLC7A11, while knockdown of BMAL1 and Nrf2 by RNA interference further downregulated the expression of these protein and thus promoting ferroptosis in response to alcohol. …”
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Association Study between Novel CYP26 Polymorphisms and the Risk of Betel Quid-Related Malignant Oral Disorders by Shyh-Jong Wu, Yun-Ju Chen, Tien-Yu Shieh, Chun-Ming Chen, Yen-Yun Wang, Kun-Tsung Lee, Yueh-Ming Lin, Pei-Hsuan Chien, Ping-Ho Chen

“…Additionally, we observed a significant mRNA expression of CY26A1 and CYP26B1 in cancerous tissues compared with adjacent noncancerous tissues. …”
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Evaluation of a new human immunodeficiency virus antigen and antibody test using light-initiated chemiluminescent assay by Yijun Li, Fangfang Jin, Yunhui Li, Yan Li, Yajie Wang, Ximing Yang

“…In 13 seroconversion panels, LiCA® detected reactive results on average 5.73 days (95% CI: 3.42–8.04) after the initial RNA test results were confirmed positive, which was 1.27 days earlier (−3.75 to 1.21) compared to Architect®. …”
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No-tillage practice enhances soil total carbon content in a sandy Cyperus esculentus L. field by Cong Wang, Yuxiang Hu, Hui Wu, Zhirui Wang, Jiangping Cai, Heyong Liu, Wei Ren, Ning Yang, Zhengwen Wang, Yong Jiang, Hui Li

“…The variations in soil bacterial community structure were investigated by 16S rRNA amplicon sequencing. The functionality of soil bacterial community was predicted based on OTU tables by using PICRUSt2. …”
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骨肉瘤细胞外泌体激活肺组织成纤维细胞炎性信号通路 by 谢显彪, 温丽丽, 吕东明, 李洪波, 刘伟海, 邹雨桐, 姚浩, 沈靖南

“…结果143B细胞外泌体中TSG101、Flotillin-1、CD63、CD9表达，Calnexin表达缺失(<italic>P</italic>&lt;0.05)；电镜外泌体呈“茶托”样形状，大小为(141.92±52.85) nm；外泌体经裸鼠尾静脉注射后进入肺组织含量显著高于肝、肾、脾、脑等器官（<italic>P</italic>&lt;0.05）；外泌体与人肺成纤维细胞共培养后炎性因子IL-1β、IL-6及TNF-α mRNA水平明显升高（<italic>P</italic>&lt;0.05），同时p-p65，p-ERK及p-p38均显著上调（<italic>P</italic>&lt;0.05）。…”
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Production of β-lactoglobulin (BLG) gene knock- out blastocyst stage embryos of Indian water buffalo using CRISPR and SCNT technology by Aseem Tara, Priyanka Singh, Devika Gautam, Gaurav Tripathi, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

“…Among three sgRNAs, the most efficient sgRNA was used to generate the clonal population of edited cells. …”
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