Huanglian Ejiao Decoction Alleviates Ulcerative Colitis in Mice Through Regulating the Gut Microbiota and Inhibiting the Ratio of Th1 and Th2 Cells by Tang J, Hu Y, Fang J, Zhu W, Xu W, Yu D, Zheng Z, Zhou Q, Fu H, Zhang W

“…Transcriptome sequencing was performed on colon tissue and 16S rRNA gene sequencing was enforced on intestinal contents.Results: The administration of HED enhanced the survival rate of colitis mice, significantly restored body weight, DAI score, colon weight and index, spleen weight and index. …”
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Expression and Clinical Significance of DAP3 in Breast Cancer and Effect of Bruceine D on Expression of DAP3:Analysis Based on Data-mining from Bioinformatics by Wenlong WANG, Qingzhi KONG, Hongda LU, Qingdong XIANG, Dianlei LIU

“…In addition, CCLE database analysis indicated that DAP3 was also highly expressed in breast cancer cell lines and its mRNA expression ranked ahead in all kinds of cancer cell lines. …”
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Prognostic significance of PD‐L2 expression in patients with oral squamous cell carcinoma—A comparison to the PD‐L1 expression profile by Manuel Weber, Falk Wehrhan, Christoph Baran, Abbas Agaimy, Maike Büttner‐Herold, Marco Kesting, Jutta Ries

“…Moreover, it should be analyzed if PD‐L2 is differentially expressed in tissue and blood samples of OSCC patients compared to healthy controls and if there is an association of PD‐L2 expression with histomorphologic and prognostic tumor parameters. Methods PD‐L2 mRNA expression was analyzed in tumors and healthy oral mucosa specimens and in corresponding peripheral blood samples of 48 OSCC patients and 26 healthy controls using RT‐qPCR. …”
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REDD1 mediates HDM-induced nuclear-cytoplasmic translocation and release of IL-33 in airway epithelial cells by downregulating Nrf2 by Tian Luo, Wentao Ji, Yuxin Gong, Lichang Chen, Chao Liu, Dandan Zhang, Xi Li, Yanhua Lv

“…To further investigate the role of the REDD1-Nrf2-IL-33 axis in the development of asthma, we employed Nrf2 activators and inhibitors to reassess the impact of REDD1 on IL-33. Results At both mRNA and protein levels, we found that REDD1 was significantly overexpressed in samples from asthma patients (P < 0.05). …”
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Mis-localization of endogenous TDP-43 leads to ALS-like early-stage metabolic dysfunction and progressive motor deficits by Yiying Hu, Alexander Hruscha, Chenchen Pan, Martina Schifferer, Michael K. Schmidt, Brigitte Nuscher, Martin Giera, Sarantos Kostidis, Özge Burhan, Frauke van Bebber, Dieter Edbauer, Thomas Arzberger, Christian Haass, Bettina Schmid

“…Behavior was investigated by video tracking and quantitative analysis of swimming parameters. RNA sequencing was used to identify mis-regulated pathways with validation by molecular analysis. …”
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基于高通量测序技术对贵州山地野猪冬季盲肠细菌多样性分析 by 彭彩淳, 杨雄威, 曹和琴, 穆 浪, 王野影, 胡灿实, 张明明, 粟海军

“…为掌握贵州山地野猪(<i>Sus scrofa</i>)盲肠细菌菌群的结构功能、多样性以及菌群的地区和性别差异,揭示细菌的特异性及其与宿主的相互作用,采用16S rRNA高通量测序技术对12头贵州野猪盲肠细菌菌群进行分析,共获得有效序列59 246条,经优化后以97%相似度聚类得到1 256个OTU。…”
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HCV and HIV co-infection among people who inject drugs in Vietnam by Vu Toan THINH, Li LI, Dréan MATTHIEU, Van Dinh HOA, Nguyen Huu ANH, Le Minh GIANG

“…Four mutually exclusive groups were defined based on the presence of detectable HCV RNA and positive HIV confirmation. Multiple logistic regression analyses were performed to explore life-time risk behaviors of HCV mono-infection and HIV/HCV co-infection. …”
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Exploring the associations of gut microbiota with inflammatory and the early hematoma expansion in intracerebral hemorrhage: from change to potential therapeutic objectives by Haixiao Jiang, Wei Zeng, Fei Zhu, Xiaoli Zhang, Demao Cao, Aijun Peng, Hongsheng Wang

“…This study analyzes these changes and associations in order to predict and prevent hematoma expansion after emICH.MethodsThe study included 100 participants, with 70 individuals diagnosed with emICH (30 with hematoma expansion and 40 without hematoma expansion, referred to as the HE and NE groups) and 30 healthy controls matched in terms of age and gender (HC). We used 16S rRNA gene sequencing to explore the gut microbial structure and its underlying associations with blood inflammatory parameters in the HE group.ResultsOur findings showed a significant decrease in the diversity and even distribution of microorganisms in the HE group when compared to the HC and NE groups. …”
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A novel mitochondrial-related risk model for predicting prognosis and immune checkpoint blockade therapy response in uterine corpus endometrial carcinoma by Ru-Gen Liao, Jin-Hong Wang, Fan Zhang, Yu-Tong Fang, Li Zhou, Yong-Qu Zhang

“…Nevertheless, the development and application of prognostic models predicated on mitochondrial-related genes (MRGs) in UCEC remains in the exploratory stages. This study utilized RNA sequencing data and clinical information from the TCGA database to identify differentially expressed MRGs (DEMRGs) between UCEC and normal groups that are associated with overall survival (OS). …”
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H. Pylori‐Facilitated TERT/Wnt/β‐Catenin Triggers Spasmolytic Polypeptide‐Expressing Metaplasia and Oxyntic Atrophy by Lijiao He, Xiao Zhang, Shengwei Zhang, Yi Wang, Weichao Hu, Jie Li, Yunyi Liu, Yu Liao, Xue Peng, Jianjun Li, Haiyan Zhao, Liting Wang, Yang‐Fan Lv, Chang‐Jiang Hu, Shi‐Ming Yang

“…Tert knockout significantly ameliorated H. pylori‐induced SPEM and single‐cell RNA sequencing demonstrated that the Wnt/β‐Catenin pathway is suppressed in gastric cells with Tert knockout. …”
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Local Application of Semaphorin 3A Combined with Adipose-Derived Stem Cell Sheet and Anorganic Bovine Bone Granules Enhances Bone Regeneration in Type 2 Diabetes Mellitus Rats by Xiaoru Xu, Kaixiu Fang, Lifeng Wang, Xiangwei Liu, Yuchao Zhou, Yingliang Song

“…The rat ASC sheets showed stronger osteogenic ability than ASCs in vitro, as indicated by the extracellular matrix mineralization and the expression of osteogenesis-related genes at mRNA level. An ASC sheet combined with Bio-Oss® bone granules promoted bone formation in T2DM rats as indicated by microcomputed tomography (micro-CT) and histological analysis. …”
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Interaction between Angiotensin II and Insulin/IGF-1 Exerted a Synergistic Stimulatory Effect on ERK1/2 Activation in Adrenocortical Carcinoma H295R Cells by An-li Tong, Fen Wang, Yun-ying Cui, Chun-yan Li, Yu-xiu Li

“…Ang II significantly stimulated mRNA expression of CYP11B1 and CYP11B2, and such stimulatory effects were enhanced when cells were cotreated with insulin/IGF-1. …”
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Gut Microbiota as Mediator and Moderator Between Hepatitis B Virus and Hepatocellular Carcinoma: A Prospective Study by Bingren Hu, Yi Yang, Jiangqiao Yao, Ganglian Lin, Qikuan He, Zhiyuan Bo, Zhewei Zhang, Anlvna Li, Yi Wang, Gang Chen, Yunfeng Shan

“…Fecal samples were collected and subjected to 16S rRNA gene sequencing. Univariate and multivariate logistic regression was applied to identify risk characteristics. …”
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IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever by Lilian Karem Flores-Mendoza, Tania Estrada-Jiménez, Virginia Sedeño-Monge, Margarita Moreno, María del Consuelo Manjarrez, Guadalupe González-Ochoa, Lourdes Millán-Pérez Peña, Julio Reyes-Leyva

“…Amounts of interleukins IL-2, IL-4, IL-6, and IL-10, interferon- (IFN-) γ, and tumor necrosis factor- (TNF-) α were quantified by flow cytometry, and the relative expression of socs1 and socs3 mRNA was quantified by real-time RT-PCR. Results. …”
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Construction of a Novel Necroptosis-Related Signature in Rat DRG for Neuropathic Pain by Liu Y, Hao S, Hao H, Zheng G, Bing J, Kang L, Li J, Zhao H, Hao H

“…This study aims to identify a gene signature related to necroptosis in DRG that can predict neuropathic pain.Methods: The mRNA expression profiles associated with neuropathic pain (GSE24982 and GSE30691) were acquired from the Gene Expression Omnibus (GEO) database. …”
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Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in lipopolysaccharide-induced RAW264.7 cell model by Xiankang Fan, Laidi Zhang, Yangying Sun, Changyu Zhou, Qiang Xia, Lihui Du, Zhen Wu, Daodong Pan

“…In the lipopolysaccharide-induced RAW264.7 cell model, the release of NO, TNF-α, and IL-6 and the mRNA expression of inflammatory factors (TNF-α, IL-6, COX-2, and NF-κB) were significantly inhibited by these peptides. …”
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Unveiling the role of melatonin‐related gene CSNK1D in osteoclastogenesis and its implications for osteoporosis treatment by Jiewen Zhang, Shaobo Wu, Fangze Xing, Ning Kong, Yiwei Zhao, Xudong Duan, Yiyang Li, Kunzheng Wang, Run Tian, Pei Yang

“…Functional analyses showed involvement of CSNK1D in OC differentiation and inflammatory pathways. in vitro experiments confirmed CSNK1D upregulation during OC differentiation, and small interfering RNA‐mediated knockdown reduced OC marker expression and TRAP+ cell formation. in vivo, CSNK1D expression is associated with bone loss in OP rats. …”
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Impact of Polystyrene Microplastics on Soil Properties, Microbial Diversity and <i>Solanum lycopersicum</i> L. Growth in Meadow Soils by Shuming Liu, Yan Suo, Jinghuizi Wang, Binglin Chen, Kaili Wang, Xiaoyu Yang, Yaokun Zhu, Jiaxing Zhang, Mengchu Lu, Yunqing Liu

“…Physicochemical analyses revealed enhanced soil organic matter and nutrient levels, including ammonium nitrogen, phosphorus, and effective potassium. Using 16S rRNA sequencing and molecular ecological network techniques, we found that PS-MPs altered the structure and function of the rhizosphere microbial community associated with <i>S. lycopersicum</i> L. …”
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Identification and validation of a prognostic risk model based on radiosensitivity-related genes in nasopharyngeal carcinoma by Yi Li, Xinyi Hong, Wenqian Xu, Jinhong Guo, Yongyuan Su, Haolan Li, Yingjie Xie, Xing Chen, Xiong Zheng, Sufang Qiu

“…Methods: We analyzed 179 NPC samples from Fujian Cancer Hospital using RNA sequencing. Differentially expressed genes (DEGs) were identified between radiotherapy-sensitive and resistant samples. …”
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Transcriptional Profiling Underscores the Role of Preprocurement Allograft Metabolism and Innate Immune Status on Outcomes in Human Liver Transplantation by Joohyun Kim, MD, PhD, Michael T. Zimmermann, PhD, Angela J. Mathison, PhD, Gwen Lomberk, PhD, Raul Urrutia, MD, Johnny C. Hong, MD

“…We conducted clinical data analyses, histologic evaluation, and RNA sequencing of 17 consecutive human primary OLT. …”
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