Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cells Induces a Switch from Classical to Atypical Symptoms in Experimental Autoimmune Encephalomyelitis by Mónica Kurte, Javiera Bravo-Alegría, Alexander Torres, Vania Carrasco, Cristina Ibáñez, Ana María Vega-Letter, Catalina Fernández-O’Ryan, Carlos E. Irarrázabal, Fernando E. Figueroa, Rodrigo A. Fuentealba, Claudia Riedel, Flavio Carrión

“…Interestingly, regardless of time administration, MSCs significantly reduced IL-6 and also T-bet, RORγT, and Foxp3 mRNA levels in brain samples of EAE mice. The downregulation of IL-6 could restore the well-functioning of the blood-brain barrier of EAE mice, correlated with a decreased number of brain infiltrating leukocytes. …”
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Insights into <i>Solea senegalensis</i> Reproduction Through Gonadal Tissue Methylation Analysis and Transcriptomic Integration by Daniel Ramírez, Marco Anaya-Romero, María Esther Rodríguez, Alberto Arias-Pérez, Robert Mukiibi, Helena D’Cotta, Diego Robledo, Laureana Rebordinos

“…RRBS (Reduced Representation Bisulfite Sequencing) was employed to detect notable methylation variations across groups, while RNA was extracted and sequenced for differential expression analysis. …”
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Lactylation-driven TNFR2 expression in regulatory T cells promotes the progression of malignant pleural effusion by Yao Liu, Qiong Zhou, Siyu Zhang, Wenbei Peng, Haolei Wang, Xuan Xiang, Linlin Ye, Xiaoshan Wei, Zihao Wang, Qianqian Xue

“…Background Although tumor necrosis factor receptor 2 (TNFR2) has been recognized as an attractive next-generation candidate target for cancer immunotherapy, the factors that regulate the gene expression and their mechanistic effects on tumor-infiltrating regulatory T cells (Treg cells) remain poorly understood.Methods Single-cell RNA sequencing analysis was employed to analyze the phenotypic and functional differences between TNFR2+ Treg cells and TNFR2− Treg cells. …”
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The potential of urinary miR-200c-3p as a biomarker of fluorosis in rats by Ailin Li, Jian Wang, Xinyue Meng, Xu Ma, Ying Liu, Hanying Li, Zhe Mo, Rui Zhang, Lei Wu, Shirui Yan, Paula Fiorella Chacon Campoverde, Gazala Zafar, Yongzheng Ma, Wenjun Que, Zhe Li, Tuo Wang, Korto Fatti Tarnue, Junrui Pei

“…Recent studies have demonstrated a link between miRNA and fluorosis. This study aimed to evaluate the time-dose-effect relationship of miR-200c-3p in plasma, urine and cartilage of rats with drinking water fluorosis, and to explore its potential as a biomarker. …”
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Advances in CAR T cell therapy: antigen selection, modifications, and current trials for solid tumors by Safwaan H. Khan, Safwaan H. Khan, Yeonjoo Choi, Mysore Veena, Mysore Veena, John K. Lee, John K. Lee, Daniel Sanghoon Shin, Daniel Sanghoon Shin

“…Additionally, novel manufacturing approaches, such as the Sleeping Beauty transposon system, mRNA-based CAR transfection, and in vivo CAR T cell production, are discussed as scalable solution to improve the accessibility of CAR T cell therapies. …”
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Comprehensive analysis of vaginal microbiota in Chinese women with genital tuberculosis: implications for diagnosis and treatment by Zhan Zhang, Xiaonan Zong, Zhaohui Liu, Xiaoyu Dong, Huihui Bai, Linyuan Fan, Ting Li

“…Short-chain fatty acid precursor pathways, including the ko03010 ribosome pathway, ko00970 aminoacyl-tRNA synthesis, ko00230 purine metabolism, ko00240 pyrimidine metabolism, and ko00010 glycolysis gluconeogenesis pathway, were significantly upregulated in women with endometrial tuberculosis. …”
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Epigenetic Inhibitors Differentially Impact TGF-β1 Signaling Cascades in COPD Airway Smooth Muscle Cells by Karosham Diren Reddy, Dikaia Xenaki, Ian M. Adcock, Brian G. G. Oliver, Razia Zakarya

“…However, a deeper understanding of the complex mechanisms associated with DNA methylation, histone post-translational changes and RNA methylation in the context of regulatory pathways remains to be elucidated. …”
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SIGLEC11 promotes M2 macrophage polarization through AKT–mTOR signaling and facilitates the progression of gastric cancer by Chen Li, Yang Lu, Yihao Liu, Minmin Shi, Zhenggang Zhu, Jingxin Yin, Qimeng Shi, Da Fu, Zhenqiang Wang

“…Flow cytometry, mouse tumor models, patient-derived tumor organoid models, and RNA sequencing were used to explore the potential functions with the underlying mechanisms of SIGLEC11 in a coculture system of macrophages and GC cells.Results We demonstrated that SIGLEC11 was predominantly expressed in normal tissues. …”
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Clinical Characteristics of Aldosterone- and Cortisol-Coproducing Adrenal Adenoma in Primary Aldosteronism by Lu Tang, Xintao Li, Baojun Wang, Xin Ma, Hongzhao Li, Yu Gao, Liangyou Gu, Wenyuan Nie, Xu Zhang

“…In the molecular biological findings, quantitative real-time PCR analysis revealed similar CYP11B1 and CYP17A1 mRNA expressions in resected A/CPA specimens and in pure APA specimens. …”
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Inhibition of RAC1 activator DOCK2 ameliorates cholestatic liver injury via regulating macrophage polarisation and hepatic stellate cell activation by Jianli Qiu, Yitong Qu, Yinli Li, Cancan Li, Junling Wang, Lu Meng, Xiaojin Jing, Jiangping Fu, Yan Xu, Yuna Chai

“…DOCK2 knockdown was achieved by tail vein injection of adenovirus containing DOCK2-targeting shRNA. The effect of DOCK2 knockdown on cholestatic liver injury was evaluated at different time points after BDL. …”
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Engineered CAF-cancer cell hybrid membrane biomimetic dual-targeted integrated platform for multi-dimensional treatment of ovarian cancer by Yuwei Yao, Jiarui Zhang, Kexin Huang, Yingying Peng, Shuangshuang Cheng, Shuangge Liu, Ting Zhou, Jinhua Chen, Haojia Li, Yingchao Zhao, Hongbo Wang

“…Result We constructed PH20-overexpressing cancer-associated fibroblast (CAF)-cancer hybrid-cell membrane vesicles (PH20/CCM) for the dual-targeted delivery of carboplatin (CBP) and siRNA targeting p65 (sip65) loaded on the poly (dimethyl diallyl ammonium chloride) (PDDA)-modified MXene (PMXene), named PMXene@CBP-sip65 (PMCS). …”
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Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes by Maria Mesuraca, Olimpio Galasso, Leonardo Guido, Emanuela Chiarella, Stefania Scicchitano, Renaud Vatrinet, Giovanni Morrone, Heather M. Bond, Giorgio Gasparini

“…Interestingly, this decrease was mirrored by the similarly reduced expression of PPARγ, a known target of ZNF423 with anti-inflammatory and chondroprotective properties. The ZNF521 mRNA instead was abundant in all primary chondrocytes studied; the RNAi-mediated silencing of this gene significantly altered the COL2A/COL1 expression ratio, associated with the maintenance of the differentiated phenotype, in chondrocytes cultivated in alginate beads. …”
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Unveiling early-life microbial colonization profile through characterizing low-biomass maternal-infant microbiomes by 2bRAD-M by Shuwen Hou, Yuesong Jiang, Feng Zhang, Tianfan Cheng, Dan Zhao, Jilong Yao, Ping Wen, Ping Wen, Lijian Jin, Shi Huang

“…We collected breast milk samples, maternal feces, and infant meconium, comparing the results from 2bRAD-M with those from both commonly used 16S rRNA amplicon sequencing and the gold-standard whole metagenomics sequencing (WMS).ResultsThe accuracy and robustness of 2bRAD-M were demonstrated through its consistently high correlation of microbial individual abundance and low whole-community-level distance with the paired WMS samples. …”
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激活Nrf2-ARE通路改善尿毒症患者血清诱导的内皮细胞功能障碍及其机制 by 邓文婕, 张群子, 邓琼霞, 娄探奇

“…【结果】尿毒症患者血清培养的人主动脉内皮细胞有显著的功能障碍，细胞凋亡率、MDA水平增高（P < 0.05）；NO含量、P-eNOS Ser1177 /eNOS（丝氨酸1177磷酸化内皮型一氧化氮合酶，P-eNOS Ser1177 ）、SOD、 CAT及GSH的水平均降低（P < 0.05）；tBHQ预处理可降低尿毒症血清条件下人主动脉内皮细胞的凋亡率和MDA含量（P < 0.05），增加NO的产生、eNOS-mRNA表达、P-eNOS Ser1177 /eNOS、SOD、CAT和GSH的水平（P < 0.05）。…”
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Microbiome of <i>Hyalomma dromedarii</i> (Ixodida: Ixodidae) Ticks: Variation in Community Structure with Regard to Sex and Host Habitat by Nighat Perveen, Alejandro Cabezas-Cruz, Daniil Iliashevich, Lianet Abuin-Denis, Olivier Andre Sparagano, Arve Lee Willingham

“…To better understand the tick microbiome influenced by sex and host habitat, we analyzed the bacterial community of <i>H. dromedarii</i> male and female ticks collected from camel farms, livestock markets, and slaughterhouses, representing the range of major habitats in the UAE, by sequencing the 16S rRNA gene. Tick samples were collected during 2022 and 2023. …”
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Dietary Supplementation with Inulin Modulates the Gut Microbiota and Improves Insulin Sensitivity in Prediabetes by Xiaojing Wang, Tong Wang, Qian Zhang, Li Xu, Xinhua Xiao

“…Intestinal microbiota profile was evaluated using the Illumina MiSeq platform based on V3-V4 bacterial 16S rRNA gene. Results. The mean age of 49 subjects was 56.6 ± 6.9 years and BMI was 25.07 ± 3.02 kg/m2. …”
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Analysing DNA methylation and transcriptomic signatures to predict prostate cancer recurrence risk by Fahad M. Aldakheel, Hadeel Alnajran, Shatha A. Alduraywish, Ayesha Mateen, Mohammed S. Alqahtani, Rabbani Syed

“…This study employs a machine learning approach to identify DNA methylation and RNA expression biomarkers predictive of PCa recurrence using datasets from The Cancer Genome Atlas (TCGA). …”
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Topical neurokinin-1 receptor antagonism ameliorates ocular pain and prevents corneal nerve degeneration in an animal model of dry eye disease by Amirreza Naderi, Yukako Taketani, Shudan Wang, Francesca Kahale, Ann Yung, Pier Luigi Surico, Yihe Chen, Reza Dana

“…Trigeminal ganglions (TG) were collected to determine SP levels by ELISA and transient receptor potential cation channel subfamily V member 1 (TRPV1), transient receptor potential cation channel subfamily M (melastatin) member 8, c-Fos, and activating transcription factor 3 (ATF3) mRNA levels by real-time polymerase chain reaction. …”
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Development of a Cell-Based High-Throughput Screening Assay to Identify Porcine Host Defense Peptide-Inducing Compounds by Zhuo Deng, Jing Wang, Wentao Lyu, Xuwen Wieneke, Robert Matts, Xi Ma, Guolong Zhang

“…Eight compounds were subsequently observed to be comparable to sodium butyrate in inducing PBD3 mRNA expression in parental IPEC-J2 cells in the low micromolar range. …”
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Transcriptome analysis reveals the anticancer effects of fenbendazole on ovarian cancer: an in vitro and in vivo study by Xin Wang, Wenda Tian, Ning Wang, Xiangqun Yang, Zhenyan Liu, Lvzhou Li, Taoyu Zhao, Chuanlin Wang, Hongping Zhang, Hongying Yang, Yue Jia

“…Functional experiments, including Cell Counting Kit 8 (CCK-8), colony formation, and flow cytometry assays, were conducted to explore the effects of FBZ on the malignant biological behavior of A2780 and SKOV3 cells. RNA sequencing and western blotting were utilized to elucidate the underlying mechanisms by which FBZ affects cell apoptosis. …”
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