Multi-omics analysis reveals novel causal pathways in psoriasis pathogenesis by Hua Guo, Jinyang Gao, Liping Gong, Yanqing Wang

“…We discovered two pathways involving the long non-coding RNA RP11-977G19.11 and apolipoprotein F (APOF). …”
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Neo-BCV: A Novel Bacterial Liquid Complex Vaccine for Enhancing Dendritic Cell-Mediated Immune Responses Against Lung Cancer by Zilong Zhu, Zhuze Chu, Fei Fei, Chenxi Wu, Zhengyue Fei, Yuxia Sun, Yun Chen, Peihua Lu

“…Methods: We investigated the immune enhancing effect of Neo-BCV through in vivo and in vitro experiments, including flow cytometry, RNA-seq, and Western blot. Results: We have demonstrated that Neo-BCV can promote Dendritic cells (DCs) maturation and induce DCs differentiation into pro-inflammatory subgroups, significantly enhancing cytotoxic T lymphocyte (CTL)-mediated anti-tumor responses. …”
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Antibody-induced erythrophagocyte reprogramming of Kupffer cells prevents anti-CD40 cancer immunotherapy-associated liver toxicity by Florence Vallelian, Marc Pfefferlé, Irina L Dubach, Raphael M Buzzi, Elena Dürst, Nadja Schulthess-Lutz, Livio Baselgia, Kerstin Hansen, Larissa Imhof, Sandra Koernig, Didier Le Roy, Thierry Roger, Rok Humar, Dominik J Schaer

“…An effective prophylactic treatment for liver immune-related adverse events that does not suppress specific antitumor immunity remains to be found.Methods We used different mouse models and time-resolved single-cell RNA-sequencing to characterize the pathogenesis of anti-CD40 mAb induced liver toxicity. …”
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Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi007-A) from a patient with Kennedy disease by Bo Li, Yitong Yang, Yingxin Wang, Didi Shan, Jianing Li, Hongxu Wang, Xiaohan Sun, Yao Tang, Yichang Jiao, Xinbo Ji, Zexin Zhan, Bo Kong, Bo Gao, Yu Wang, Ping Sun, Fuchen Liu

“…The pluripotency of these iPSCs was verified by the expression of several pluripotency markers at both RNA and protein levels, as well as their capability to differentiate into all three germ layers.…”
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Immune gene features and prognosis in colorectal cancer: insights from ssGSEA typing by Anwen Huang, Jinxiu Wu, Jiakuan Wang, Chengwen Jiao, Yunfei Yang, Huaiwen Xiao, Li Yao

“…Methods We obtained and merged RNA-Seq data along with clinical details for colorectal cancer (CRC) from The Cancer Genome Atlas (TCGA) repository, and then performed immunocluster typing on all CRC specimens. …”
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Combination Antiretroviral Therapy for HIV in Rwandan Adults: Clinical Outcomes and Impact on Reproductive Health up to 24 Months by Brenda Asiimwe-Kateera, Nienke Veldhuijzen, Jean Paul Balinda, John Rusine, Sally Eagle, Joseph Vyankandondera, Julie Mugabekazi, Pascale Ondoa, Kimberly Boer, Anita Asiimwe, Joep Lange, Peter Reiss, Janneke van de Wijgert

“…In the cART groups, 21% of patients required a drug change due to side effects and 11% of patients had virological failure (defined as >1,000 HIV RNA copies/mL) after 12 months of cART. About a third of the pregnancies since HIV diagnosis were unintended. …”
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(-)-Epigallocatechin Gallate Reduces Platelet-Derived Growth Factor-BB-Stimulated Interleukin-6 Synthesis in Osteoblasts: Suppression of SAPK/JNK by Shinji Takai, Rie Matsushima-Nishiwaki, Seiji Adachi, Hideo Natsume, Chiho Minamitani, Jun Mizutani, Takanobu Otsuka, Haruhiko Tokuda, Osamu Kozawa

“…EGCG significantly reduced the IL-6 synthesis and IL-6 mRNA expression stimulated by PDGF-BB, EGCG reduced the PDGF-BB-stimulated IL-6 synthesis also in primary-cultured osteoblasts. …”
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Anti-SARS-Cov-2 S-RBD IgG Formed after BNT162b2 Vaccination Can Bind C1q and Activate Complement by Anas H. A. Abu-Humaidan, Fatima M. Ahmad, Dima Awajan, Raba’a F. Jarrar, Nader Alaridah

“…In this study, we investigated C1q binding and subsequent complement activation by anti-spike (S) protein receptor-binding domain (RBD) specific antibodies produced following vaccination with either the mRNA vaccine BNT162b2 or the inactivated vaccine BBIBP-CorV. …”
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Bacteria Assemble: Investigating Soil Bacterial Diversity and Interactions in a Sarawakian Oil Palm Plantation for Climate-Resilient Soil Management by Zahidah Ayob, Nor Azizah Kusai, Han Ming Gan, Wan Nurul‘Ain Wan Mohd Nor, Hasimah Mos, Vijaya Subramaniam, Meilina Ong Abdullah

“…We employed Illumina amplicon sequencing targeting the V3 region of the 16S rRNA gene, which revealed a diverse array of soil bacteria, primarily from the phyla Acidobacteriota, Actinobacteriota, and Proteobacteria. …”
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miR-155与<italic>CEBPB</italic>的靶向验证及繁殖性能相关基因的检测 by 李军义, 刘娣, 马红, 汪亮, 霍秀鹏, 郝丽

“…<italic>CEBPB</italic>具有调节黄体生成促进排卵的作用，对动物繁殖性能极为重要，试验旨在通过构建<italic>CEBPB</italic>的3'端非翻译区（3'UTR）双荧光素酶报告基因载体，验证MicroRNA-155（miR-155）调控<italic>CEBPB</italic>的分子机制。应用在线生物信息学软件预测miR-155与<italic>CEBPB</italic>基因3'UTR的靶向结合区，PCR扩增获取民猪组织基因组的<italic>CEBPB</italic>的3'UTR，将其插入psi-Check-2载体，构建野生型（wt-3'UTR）和突变型重组表达载体（mut-3'UTR），鉴定正确后，分别与miR-155模拟物（mimics）/抑制物（inhibitor）/阴性对照物（NC）共转染到PK15细胞，检测荧光素酶活性及<italic>CEBPB</italic>表达情况。…”
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The key role of the NUDT3 gene in lung adenocarcinoma progression and its association with the manganese ion metabolism family by Deyong Ge, Xinyu Xu, Liyi Fang, Huihui Tao

“…Quantitative PCR (qPCR) was conducted to evaluate the relative mRNA expression of NUDT3 in A549 lung adenocarcinoma cell lines compared to BEAS-2B normal bronchial epithelial cell lines. …”
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GPX3 as a Novel and Potential Therapeutic Target in the Shared Molecular Mechanisms of Traumatic Brain Injury and Parkinson&rsquo;s Disease by Wang Y, Fang J, Yuan Q, Yu J, Hu J

“…Further analyses, including GO and KEGG pathway enrichment, protein-protein interaction (PPI) network construction, hub gene identification, as well as miRNA and transcription factor prediction and drug candidate screening, were conducted. …”
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Yeokwisan: Standardised Herbal Formula Enhancing Gastric Mucosal Protection Against Gastric Ulcers in Mice, a Preclinical Study by Yun Mi Lee, Kyuhyung Jo, So Yeon Kim, Chang-Seob Seo, Eunjung Son, Aejin Kim, Dong-Seon Kim

“…YWS showed gastroprotective effects through histamine downregulation, while prostaglandin E2 (PGE2) and mucin were upregulated. The mRNA levels of H2R, M3R, CCK2R, and H⁺/K⁺ ATPase were significantly decreased following treatment with YWS. …”
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IL-33 Protects Mice against DSS-Induced Chronic Colitis by Increasing Both Regulatory B Cell and Regulatory T Cell Responses as Well as Decreasing Th17 Cell Response by Jun-feng Zhu, Ying Xu, Jian Zhao, Xue Li, Xinrui Meng, Tian-qi Wang, Ben-yao Zou, Peng-yan Zhao, Qi Liu, Chang-long Lu, Fang-liang Zheng, Hong-sheng Liu

“…The T and B cell subsets were measured by flow cytometry. The expression of mRNA of transcription factors was measured by quantitative real-time PCR. …”
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Effect of Kinases in Extracellular Vesicles from HIV-1-Infected Cells on Bystander Cells by Gifty A. Mensah, Anastasia Williams, Pooja Khatkar, Yuriy Kim, James Erickson, Alexandra Duverger, Heather Branscome, Kajal Patil, Hafsa Chaudhry, Yuntao Wu, Olaf Kutsch, Fatah Kashanchi

“…Recently, we have found that exosomes, which are small, membrane-enclosed particles released by virtually all cell types and known to mediate intercellular communication, caused an increase in RNA Polymerase II loading onto the HIV-1 promoter. …”
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Resistance to medically important antimicrobials in broiler and layer farms in Cameroon and its relation with biosecurity and antimicrobial use by Ronald Vougat Ngom, Ronald Vougat Ngom, Andrea Laconi, Roberta Tolosi, Adonis M. M. Akoussa, Stephane D. Ziebe, Vincent M. Kouyabe, Alessandra Piccirillo

“…Additionally, the study examined the relationship between these ARGs and biosecurity implementation, as well as farmers’ knowledge, attitudes, and practices toward antimicrobial use (AMU) and AMR, including their perception of AMR risk.Materials and methodsFecal, drinking water, and biofilm samples from drinking water pipelines were collected from 15 poultry farms and subsequently analyzed by real-time PCR and 16S rRNA NGS.ResultsAll samples tested positive for genes conferring resistance to (fluoro) quinolones, 97.8% to macrolides, 64.4% to polymyxins, and 11.1% to carbapenems. …”
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人工养殖西伯利亚狍肠道微生物组成的雌雄差异 by 刘金月 刘德凯 王璐瑶 李响 贾博文 刘艳华

“…本研究利用高通量测序技术在Illumina MiSeq平台上对14只狍肠道微生物进行16S rRNA基因分析。结果表明：个体样本中门类组成的差异，雌性个体JY3和雄性个体JY9除Firmicutes，Bacteroidetes，Proteobacteria这3类菌群外，其他门类菌群所占比例都很低。…”
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Fever, Myositis, and Paralysis: Is This Inflammatory Myopathy or Neuroinvasive Disease? by Aneeta R. Kiran, Richard A. Lau, Kim M. Wu, Andrew L. Wong, Philip J. Clements, Emil R. Heinze

“…West Nile virus (WNV) is a mosquito-borne RNA Flavivirus which emerged in North America in 1999. …”
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Integrative analysis of ferroptosis in the hypoxic microenvironment of gastric cancer unveils the immune landscape and personalized therapeutic strategies by Xiao Xu, Liangling Fa, Xiaoxiao Sun, Fangfang Yang, Yongrui Liu, Jifu Song, Yongli Zhao, Jigang Dong, Jigang Dong

“…The predictive role of ferroptosis in gastric cancer (GC) hypoxia TME, particularly in relation to TME immune cell infiltration, has not been fully explained.MethodsBy analyzing the mRNA expression data of ferroptosis and hypoxia-related genes, a prediction model was constructed to evaluate further the predictive value of immune cell infiltration, clinical characteristics, and immunotherapy efficacy of gastric cancer, and the essential genes were validated.ResultsTwo distinct molecular states of ferroptosis-hypoxia were identified in GC. …”
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FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids by Simone Isling Pærregaard, Marianne Agerholm, Annette Karen Serup, Tao Ma, Bente Kiens, Lise Madsen, Karsten Kristiansen, Benjamin Anderschou Holbech Jensen

“…Moreover, ω3-PUFA fed mice had increased circulating protein levels of the anti-inflammatory adipokine, adiponectin, decreased fasting insulin levels, and decreased mRNA expression of several proinflammatory molecules within visceral adipose tissue. …”
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