Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKKβ-AMPK-SIRT1 Signaling Pathway by Xin Liu, Ning Wang, Yuanfeng Zhu, Yongjun Yang, Xiaoli Chen, Shijun Fan, Qian Chen, Hong Zhou, Jiang Zheng

“…Chemical inhibitor or siRNA of SIRT1 enhanced IL-12 release while its agonist suppressed IL-12 production. …”
Get full text

Mgl2+ cDC2s coordinate fungal allergic airway type 2, but not type 17, inflammation in mice by Peter C. Cook, Sheila L. Brown, Emma L. Houlder, Julio Furlong-Silva, Daniel P. Conn, Stefano A. P. Colombo, Syed Baker, Freya R. Svedberg, Gareth Howell, Margherita Bertuzzi, Louis Boon, Joanne E. Konkel, Christopher R. Thornton, Judith E. Allen, Andrew S. MacDonald

“…Here we find that CD11c+ DCs and CD4+ T cells are essential for development of both type 2 and type 17 airway inflammation in mice repeatedly exposed to inhaled spores. Single cell RNA-sequencing with further multi-parameter cytometry shows that allergic inflammation dramatically alters the proportion of numerous DC clusters in the lung, but that only two of these (Mgl2+ cDC2s and CCR7+ DCs) migrate to the dLNs. …”
Get full text

Genome-wide analysis of Smad and Schnurri transcription factors in C. elegans demonstrates widespread interaction and a function in collagen secretion by Mehul Vora, Jonathan Dietz, Zachary Wing, Karen George, Jun Kelly Liu, Christopher Rongo, Cathy Savage-Dunn

“…Here, we identify functional binding sites for SMA-3/Smad and its transcriptional partner SMA-9/Schnurri based on ChIP-seq peaks (identified by modEncode) and expression differences of nearby genes identified from RNA-seq analysis of corresponding mutants. We found that SMA-3 and SMA-9 have both overlapping and unique target genes. …”
Get full text

Immunological Aspects of Fulminant Type 1 Diabetes in Chinese by Zhen Wang, Ying Zheng, Yiting Tu, Zhijie Dai, Jian Lin, Zhiguang Zhou

“…And peripheral T cell responses to GAD65, insulin B9–23 peptide, or C peptide were determined in 10 FT1D patients and 10 healthy controls. The mRNA levels of several related cytokines and molecules, such as IFN-γ, IL-4, RORC, and IL-17 in PBMCs from FT1D patients were analyzed by qRT-PCR. …”
Get full text

Genetically engineered biomimetic ATP-responsive nanozyme for the treatment of cardiac fibrosis by Xueli Zhao, Yuze Qin, Bowen Li, Yue Wang, Jiao Liu, Bo Wang, Jia Zhao, Jiaqi Yin, Lanlan Zhang, Jing Li, Junzhe Huang, Kun Chen, Liwen Liu, Yuanming Wu

“…By fusing the anti-FAP CAR genetically engineered cell membrane to zeolitic imidazole frameworks-90 (zif-90) cores loaded with antioxidant nanozymes CeO2 and siCTGF (siRNA targeting CTGF), these nanoparticles, called FM@zif-90/Ce/siR NPs, are demonstrated to effectively reduce the accumulation of myofibroblasts and the formation of fibrotic tissue, while restoring cardiac function. …”
Get full text

Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-κB-Dependent Mechanism in HaCaT Keratinocytes by Bing-rong Zhou, Jia-an Zhang, Qian Zhang, Felicia Permatasari, Yang Xu, Di Wu, Zhi-qiang Yin, Dan Luo

“…Secretion levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), NF-κB nuclear translocation, NF-κB activation, Stat3 phosphorylation, and peroxisome proliferator-activated receptor alpha (PPARα) mRNA and protein levels, as well as the cell proliferation ability were measured at the end of the treatment and after 24 hours of recovery. …”
Get full text

Preparation and In Vivo Expression of CS-PEI/pCGRP Complex for Promoting Fracture Healing by Chun-Liang Li, Feng Qin, Rong-rong Li, Jia Zhuan, Hai-Yong Zhu, Yu Wang, Kai Wang

“…Moreover, CS-PEI/pCGRP significantly enhanced the mRNA and protein levels of downstream RUNX2 and ALP. …”
Get full text

Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome by Juan Du, Yuan Wang, Shaohui Su, Wenwen Wang, Tao Guo, Yuchao Hu, Na Yin, Xiaoping An, Jingwei Qi, Xuan Xu

“…Our findings suggest that adding CPS to lambs’ diets has widespread positive impacts, including improved growth performance, reduced inflammation and mRNA relative expression of apoptosis-related genes, enhanced barrier function, and beneficial changes in the rumen microbiome and metabolite profiles.…”
Get full text

CTLA-4 and PD-1 Ligand Gene Expression in Epithelial Thyroid Cancers by Chiara Tuccilli, Enke Baldini, Salvatore Sorrenti, Antonio Catania, Alessandro Antonelli, Poupak Fallahi, Francesco Tartaglia, Susi Barollo, Caterina Mian, Andrea Palmieri, Giovanni Carbotta, Stefano Arcieri, Daniele Pironi, Massimo Vergine, Massimo Monti, Salvatore Ulisse

“…Here, the expression of PD-L1, PD-L2, CD80, and CD86 was evaluated at the mRNA level in 94 patients affected by papillary thyroid carcinoma (PTC) and 11 patients affected by anaplastic thyroid carcinoma (ATC). …”
Get full text

Occurrence and Multi-Locus Genotyping of <i>Giardia duodenalis</i> in Black Goats from Fujian Province, China by Shou-Xiao Huang, Kai Hu, Peng-Fei Fu, Si-Ang Li, Yang Liu, Zhipeng Niu, Dong-Hui Zhou

“…The presence of <i>G. duodenalis</i> was confirmed through nested PCR targeting the SSU rRNA gene, and genotyping was performed at the beta-giardin, glutamate dehydrogenase, and triosephosphate isomerase loci. …”
Get full text

Effects of Neural Stem Cells combine with Nerve Growth Factor Nanoparticles Transplantation in Alzheimer's Disease Transgenic Mice by Qing ZHU, Shuai SHAO, Nan HU, Yan CHEN

“…Real-time quantitative PCR(Q-PCR)was used to detect the mRNA expressions of ChAT and Lhx8 in the basal forebrain. …”
Get full text

Early and delayed STAT1-dependent responses drive local trained immunity of macrophages in the spleen by Aryeh Solomon, Noa Bossel Ben-Moshe, Dotan Hoffman, Sébastien Trzebanski, Dror Yehezkel, Leia Vainman, Mihai G Netea, Roi Avraham

“…Here, we study the effects of peritoneal Bacillus Calmette–Guérin (BCG) administration to find TI-mediated protection in the spleen against a subsequent heterologous infection by the Gram-negative pathogen Salmonella Typhimurium (S.Tm). Utilizing single cell RNA-sequencing and flow cytometry, we discerned STAT1-regulated genes in TI-associated resident and recruited splenic myeloid populations. …”
Get full text

ZBP1 senses Brucella abortus DNA triggering type I interferon signaling pathway and unfolded protein response activation by Marco Túlio R. Gomes, Erika S. Guimarães, Erika S. Guimarães, Sergio C. Oliveira, Sergio C. Oliveira, Sergio C. Oliveira

“…The knockdown of STING by siRNA decreased the residual IFN-β signal elicited by B. abortus infection, demonstrating the presence of a redundant cytosolic DNA-sensing mechanism driving type I IFN production. …”
Get full text

Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-Myc/RNMT Axis by Hanqing Chen, Yundi Guo, Jing Sun, Jun Dong, Qinghua Bao, Xueguang Zhang, Fengqing Fu

“…Moreover, we determined that three genes CBL (Casitas B-Lineage Lymphoma Proto-Oncogene), CCNT1 (Cyclin T1), and RNMT (RNA guanine-7 methyltransferase) were coexpressed with B7-H6 and c-myc in glioma tissue samples from the TCGA database and found, however that only RNMT expression was inhibited by the knockdown of B7-H6 expression in the GSLCs, suggesting the involvement of RNMT in the B7-H6/c-myc axis. …”
Get full text

Targeting OAS3 for reversing M2d infiltration and restoring anti-tumor immunity in pancreatic cancer by Shaopeng Zhang, Ximo Xu, Kundong Zhang, Changzheng Lei, Yitian Xu, Pengshan Zhang, Yuan Zhang, Haitao Gu, Chen Huang, Zhengjun Qiu

“…Increased OAS3 expression in TAMs correlates with m6A modification mediated by METTL3 on OAS3 mRNA and is associated with poorer prognosis in PC patients. …”
Get full text

Osteoarthritis-like changes in rat temporomandibular joint induced by unilateral anterior large overjet treatment by Xinru Xie, Rui Chao, Yi Mao, Tianhao Wan, Yexin Wang, Yan Zhu, Weifeng Xu, Xuzhuo Chen, Yong Wang, Zhigui Ma, Shanyong Zhang

“…By inducing a posteriorly positioned condyle, the UALO device effectively results in cartilage degradation, subchondral bone loss, condylar volume reduction, and mandibular retrusion. Furthermore, RNA sequencing of condylar cartilages revealed that the oxidative stress of chondrocytes was elevated under the UALO-triggered abnormal mechanical stress. …”
Get full text

Variations in rumen microbiota and host genome impacted feed efficiency in goat breeds by Alaa Emara Rabee, Ibrahim Abou-souliman, Ahmed I. Yousif, Mebarek Lamara, Mohamed A. El-Sherbieny, Eman Ali Elwakeel, Ahmed M. Sallam

“…The animals were genotyped using the Illumina 65KSNP chip arrays v2, and their rumen bacteria and archaea were investigated using PCR-amplicon sequencing of the 16S rRNA gene.ResultsThe results showed that the ZA goats exhibited higher FE than the SH goats (p &lt; 0.05) and a greater relative abundance (p &lt; 0.05) of rumen bacterial groups that are involved in the degradation of cellulose and hemicelluloses, such as Bacteroidota and Fibrobacterota, along with genera such as Prevotella, Lachnospiraceae AC2044 group, Lachnospiraceae NK3A20 group, and Succiniclasticum, which are linked to carbohydrate and nitrogen metabolism pathways. …”
Get full text

Low-Dose Mitomycin C Decreases the Postoperative Recurrence Rate of Pterygium by Perturbing NLRP3 Inflammatory Signalling Pathway and Suppressing the Expression of Inflammatory Fac... by Qie Guo, Xiao Li, Meng-Na Cui, Yu Liang, Xiang-Peng Li, Jun Zhao, Li-Na Wei, Xiao-Lei Zhang, Xiang Hua Quan

“…Mitomycin C (MMC) is an antineoplastic antibiotic that inhibits DNA, RNA, and protein synthesis. In recent years, although MMC has proven useful for the treatment of pterygium, its application has been controversial because of its clear toxicity and the possibility of ocular complications. …”
Get full text

Impaired fracture healing is associated with callus chondro-osseous junction abnormalities in periostin-null and osteopontin-null mice by Marc Teitelbaum, Marc Teitelbaum, Maya D. Culbertson, Charlene Wetterstrand, J. Patrick O’Connor, J. Patrick O’Connor

“…Apparent changes in specific protein levels identified by immunohistochemistry were confirmed by mRNA quantitation. Comparisons between the PostnKO and Spp1KO fracture calluses were confounded by interactions between the two genes; loss of Postn reduced Spp1 expression and loss of Spp1 reduced Postn expression. …”
Get full text

Single-cell sequencing provides clues about the developmental genetic basis of evolutionary adaptations in syngnathid fishes by Hope M Healey, Hayden B Penn, Clayton M Small, Susan Bassham, Vithika Goyal, Micah A Woods, William A Cresko

“…We created a single-cell RNA sequencing atlas of Gulf pipefish embryos to understand the developmental basis of four traits: derived head shape, toothlessness, dermal armor, and male pregnancy. …”
Get full text