Exercise-induced methylation of the Serhl2 promoter and implication for lipid metabolism in rat skeletal muscle by Mutsumi Katayama, Kazuhiro Nomura, Jonathan M. Mudry, Alexander V. Chibalin, Anna Krook, Juleen R. Zierath

“…Additional methods included promoter luciferase assays, fluorescence microscopy, and RNA/DNA analysis to investigate exercise-induced molecular changes. …”
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Construction and Characterization of Adenovirus Vectors Encoding Aspartate-β-Hydroxylase to Preliminary Application in Immunotherapy of Hepatocellular Carcinoma by Yujiao Zhou, Feifei Liu, Chengmin Li, Guo Shi, Xiaolei Xu, Xue Luo, Yuanling Zhang, Jingjie Fu, Aizhong Zeng, Limin Chen

“…Firstly, the total AAH mRNA was extracted from human HCC tissues; the cDNA was amplified by RT-PCR, verified, and sequenced after TA cloning. …”
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The Progression of Mycosis Fungoides During Treatment with Mogamulizumab: A BIO-MUSE Case Study of the Tumor and Immune Response in Peripheral Blood and Tissue by Angelica Johansson, Eirini Kalliara, Emma Belfrage, Teodor Alling, Paul Theodor Pyl, Anna Sandström Gerdtsson, Urban Gullberg, Anna Porwit, Kristina Drott, Sara Ek

“…<b>Methods:</b> We applied 10× genomics-based single-cell RNA sequencing to CD3+ peripheral T-cells, combined with T-cell receptor sequencing, to samples collected at multiple timepoints during the progression of the disease. …”
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Improving natural red pigment production by Streptomyces phaeolivaceus strain GH27 for functionalization of textiles with in silico ADME prediction by Gehad H. El Sayed, Mohamed Fadel, Rasha Fouad, Hend M. Ahmed, Ahmed A. Hamed

“…Abstract The red pigment was recovered from the S. phaeolivaceus GH27 isolate, which was molecularly identified using 16S rRNA gene sequencing and submitted to GenBank as OQ145635.1. …”
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Significant Contribution of Mouse Mast Cell Protease 4 in Early Phases of Experimental Autoimmune Encephalomyelitis by Louisane Desbiens, Catherine Lapointe, Marjan Gharagozloo, Shaimaa Mahmoud, Gunnar Pejler, Denis Gris, Pedro D’Orléans-Juste

“…Finally, whereas EAE triggered significant increases in brain levels of mMCP-4 mRNA and immunoreactive ET-1 in WT mice, the latter peptide was reduced to basal levels in mMCP-4 KO congeners. …”
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Methylsulfonylmethane (MSM) Supplementation in Adult Horses Supports Improved Skeletal Muscle Inflammatory Gene Expression Following Exercise by Madison R. Barshick, Kristine M. Ely, Keely C. Mogge, Lara M. Chance, Sally E. Johnson

“…Skeletal muscle biopsies were retrieved from the middle gluteus before and 1 h post-SET for total RNA isolation. All horses were rested for 120 days before the experiment was repeated in a cross-over design. …”
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LINC01271 promotes fracture healing via regulating miR-19a-3p/PIK3CA axis by Qinghua Chen, Lina Huang, Wenjun Ji, Miao Huang, Jincheng Sima, Jin Li, Hao Song, Wei Xiong, Bin Chen

“…Luciferase reporter assays, RIP experiments, and RNA pull-down assays were utilized to verify the target relationships between LINC01271 and miR-19a-3p, as well as between miR-19a-3p and PIK3CA. …”
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Lake Baikal amphipods and their genomes, great and small by P. B. Drozdova, E. V. Madyarova, A. N. Gurkov, A. E. Saranchina, E. V. Romanova, J. V. Petunina, T. E. Peretolchina, D. Y. Sherbakov, M. A. Timofeyev

“…Studies of mitochondrial genomes revealed some unusual features, such as variation in length and gene order, as well as duplications of tRNA genes, some of which also underwent remolding (change in anticodon specificity due to point mutations). …”
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Expression of genes of cytokines, transcription factors and differentiation antigens in human dendritic cells activated by double-stranded DNA by A. S. Proskurina, K. E. Orishchenko, E. A. Potter, E. V. Dolgova, A. V. Spaselnikova, G. S. Ritter, N. A. Varaksin, T. G. Ryabicheva, O. Y. Leplina, A. A. Ostanin, E. R. Chernykh, S. S. Bogachev

“…It was for the first time shown that genomic “self” double-stranded DNA as a mono agent activates mRNA synthesis of cytokines IFN-α, IFN-β, IFN-γ, IL­8, IL­10, and VEGF in DCs at 6 hours of induction.…”
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Administration of a Probiotic Mixture Ameliorates Cisplatin-Induced Mucositis and Pica by Regulating 5-HT in Rats by Yuanhang Wu, Jianlin Wu, Zhikun Lin, Qian Wang, Ying Li, Aman Wang, Xiu Shan, Jiwei Liu

“…The structure of the gut microbiota was analyzed by 16S rRNA gene sequencing and quantitative PCR. Additionally, 5-HT levels in enterochromaffin (EC) cells (RIN-14B cell line) treated with cisplatin and/or various probiotic bacteria were also determined. …”
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Metagenomic analysis of gut microbiota diversity in poultry before and after antibiotic administration by O. V. Prasolova, N. I. Malik, I. A. Timofeeva, N. A. Kirsanova, E. V. Krylova, E. V. Malik, I. A. Rusanov, N. A. Chupakhina

“…This study gives a characteristic of poultry gut microbiota diversity before and after antibiotic administration based on 16S rRNA gene sequencing analysis. Firmicutes and Bacteroidota species were predominantly detected in the chick microbiota during antibiotic administration and after its withdrawal. …”
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Correlation Analysis between GDM and Gut Microbial Composition in Late Pregnancy by Genxia Li, Pan Yin, Shuhui Chu, Wanli Gao, Shihong Cui, Shuhua Guo, Yajuan Xu, Enwu Yuan, Texuan Zhu, Jie You, Junya Zhang, Manman Yang

“…We sequenced the V3-V4 regions of the 16S ribosomal ribonucleic acid (rRNA) gene from stool samples of 52 singleton pregnant women at >28 weeks of gestation. …”
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Elevated Urinary Connective Tissue Growth Factor in Diabetic Nephropathy Is Caused by Local Production and Tubular Dysfunction by Karin G. F. Gerritsen, Jan Willem Leeuwis, Maarten P. Koeners, Stephan J. L. Bakker, Willem van Oeveren, Jan Aten, Lise Tarnow, Peter Rossing, Jack F. M. Wetzels, Jaap A. Joles, Robbert Jan Kok, Roel Goldschmeding, Tri Q. Nguyen

“…FE of recombinant CTGF correlated with excretion of endogenous CTGF. CTGF mRNA was mainly localized in glomeruli and medullary tubules. …”
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Investigating the Effect and Potential Mechanism of Rhamnetin 3-<i>O</i>-α-Rhamnoside on Acute Liver Injury In Vivo and In Vitro by Dandan Deng, Borong Zhao, Hong Yang, Songsong Wang, Ziying Geng, Jiangtao Zhou, Guane Yang, Liwen Han

“…The hepatoprotective activity of ARR was evaluated by assessing liver morphology, liver function indices, oxidative stress, and the mRNA expression levels of inflammation-related genes in the zebrafish model. …”
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Th17/Treg balance is regulated during the suppression of experimental autoimmune encephalomyelitis treated by Astragalus polysaccharides via the microbiota-gut-brain axis by Jinyun Ma, Xiaojun Liu, Yan Zhao, Qijin Lu, Guiqing Ding, Yuanhua Wang, Xiaodong Cheng

“…The experiment with an antibiotic cocktail interfering with the gut microbiota proved that APS alleviated EAE by regulating the gut microbiota. Through 16S rRNA sequencing, we showed that APS regulated gut microbiota diversity and composition in EAE mice. …”
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Clinical Activity of Behcet&rsquo;s Disease Associated with SARS-CoV-2 and Herpes Simplex Virus: Insights from Three Case Reports by Bakti FU, Sufiawati I

“…The first patient was confirmed to have COVID-19 by the SARS-CoV-2 RNA test. The second and third patients had a two-year history of recurrent oral ulcerations with reactive IgG anti-HSV-1 tests. …”
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Pan-Cancer Analysis of Disulfidptosis-Related Genes Affecting Prognosis and Tumor Microenvironment by Jingyang SUN, Rongxuan JIANG, Liren HOU, Huanhuan DONG, Yihan LIN, Niuniu DONG, Guangjian ZHANG, Yanpeng ZHANG

“…ObjectiveTo assess the potential role of disulfidptosis-related genes (DRGs) in pan-cancer on prognosis and immunity on the basis of bioinformatics approaches. MethodsPan-cancer RNA-seq data, mutation profiles, clinical information, TMB, MSI, stemness scores, and tumor and immune microenvironment data contained in TCGA and various open-source online databases, and multi-group R-language algorithms were used for comprehensive analysis. …”
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Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese by Ning Zhou, Yaoyao Zhang, Youluan Jiang, Wang Gu, Shuai Zhao, Wanwipa Vongsangnak, Yang Zhang, Qi Xu, Yu Zhang

“…Antioxidant activity (GSH, CAT, SOD, T-AOC, and the content of H₂O₂) and the mRNA expression levels of antioxidant genes (GPX, SOD-1, SOD-2, CAT, COX-2, and Hsp70) were significantly higher in pre-broody geese compared to laying geese, while the expression of apoptosis-related genes (p53, Caspase-3, and Caspase-9) increased and the anti-apoptotic gene Bcl-2 decreased. …”
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C9ORF72 poly-PR induces TDP-43 nuclear condensation via NEAT1 and is modulated by HSP70 activity by Diksha Agnihotri, Chi-Chang Lee, Po-Chao Lu, Ruei-Yu He, Yung-An Huang, Hung-Chih Kuo, Joseph Jen-Tse Huang

“…In this study, we find that the poly-PR dipeptide enhances the formation of TDP-43 NCs with decreased fluidity. While the non-coding RNA, nuclear-enriched abundant transcript 1 (NEAT1), is essential for the formation of TDP-43 NCs, heat shock protein 70 (HSP70) chaperone maintains their fluidity. …”
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A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle by Nugzar G. Bakradze, Marina K. Abuladze, Victor M. Sokhadze, Nina V. Asatiani, Nelly A. Sapojnikova, Tamara M. Kartvelishvili, Emma N. Namchevadze, Nelly Y. Tsibakhashvili, Leila V. Tabatadze, Lia V. Lejava, Hoi-Ying Holman

“…This may correspond to the orderly changes in subcellular structural elements: proteins, ribosomes and RNA, membranes, and various structural elements of the cell wall during different points of the growth cycle and growth conditions. …”
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