The effects of Bifidobacterium animalis QC08 on reducing uric acid level and providing renal protection in mice with hyperuricemia by Huijia Mao, Yang Fan, Fang Tan, Xingyao Long

“…Additionally, Bifidobacterium animalis QC08 was found to regulate the mRNA transcription of renal uric acid transporters, leading to significantly upregulation of the expression levels of UAT, ABCG2, and OAT1 genes (p < 0.05).ConclusionBifidobacterium animalis QC08 demonstrates certain uric acid-lowering, anti-inflammatory, and renal protective effects, which are associated with the inhibition of XOD activity and the modulation of the expression levels of uric acid transporter genes (UAT, ABCG2, and OAT1).…”
Get full text

Se-methylselenocysteine inhibits inflammatory response in an LPS-stimulated chicken HD11 macrophage-like cell model through the NFKB2 pathway by Min Yao, Binyu Wang, Zitong Li, Suqing Wu, Bingyu Zhao, Ning Sun, Huiping Xiao, Jianwu Wang, Guoping Liu, Tinghua Huang

“…In this study, we employed an LPS-stimulated chicken HD11 macrophage-like cell model to identify the principal transcription factors involved in transcriptome regulation responsible for SeMC treatment. RNA-seq identified 3,263 transcripts that exhibited a statistically significant differential expression between the SeMC-treated group and the control group and 1,344 transcripts that exhibited a statistically significant differential expression between the LPS + SeMC- and LPS-treated groups (FDR < 0.05, FDR > 1.5). …”
Get full text

NLS-binding deficient Kapβ2 reduces neurotoxicity via selective interaction with C9orf72-ALS/FTD dipeptide repeats by Kevin M. Kim, Amandeep Girdhar, Maria E. Cicardi, Vaishnavi Kankate, Miyuki Hayashi, Ruoyu Yang, Jenny L. Carey, Charlotte M. Fare, James Shorter, Gino Cingolani, Davide Trotti, Lin Guo

“…R-DPRs can cause toxicity by disrupting the natural phase behavior of RNA-binding proteins (RBPs). Mitigating this abnormal phase behavior is, therefore, crucial to reduce R-DPR-induced toxicity. …”
Get full text

Interaction of the Wolbachia surface protein with a novel pro-viral protein from Aedes aegypti by Mazhar Hussain, Zhi Qi, Sassan Asgari

“…Silencing of STK, but not SVM, reduced DENV replication in Aag2 cells and mosquitoes. Conversely, RNA activation of STK, by utilizing promoter induction via short activating oligos, resulted in higher DENV replication in Wolbachia-infected and uninfected cell lines. …”
Get full text

Characterization of the Cannabis sativa glandular trichome epigenome by Lee J. Conneely, Bhavna Hurgobin, Sophia Ng, Muluneh Tamiru-Oli, Mathew G. Lewsey

“…Corresponding transcriptomic (RNA-seq) datasets were integrated, and tissue-specific analyses conducted to relate chromatin states to glandular trichome specific gene expression. …”
Get full text

CFTR氯通道调控小鼠内毒素血症血小板活化及炎症因子生成 by 胡瑞, 周仪, 李梅, 王冠蕾

“…结果<italic>Cftr</italic>^+/+小鼠静脉注射LPS后，外周血血小板TLR4和P-selectin表达增加、血小板数目减少、血小板表达促炎炎症因子mRNA水平升高，而CFTR全基因敲除加重之（<italic>P</italic>&lt;0.05）。…”
Get full text

A fishy gut feeling – current knowledge on gut microbiota in teleosts by Ingvill Tolas, Ingvill Tolas, Zhigang Zhou, Zhen Zhang, Zhen Zhang, Tsegay Teame, Tsegay Teame, Rolf Erik Olsen, Einar Ringø, Ivar Rønnestad

“…Although less is known in fish than in mammals, advances in molecular techniques, such as 16S rRNA sequencing, have facilitated characterization of fish GM, comprising resident autochthonous and transient allochthonous bacteria. …”
Get full text

Prevalence and Residual Risk of HIV in Volunteer Blood Donors of Zhejiang Province, China, from 2018 to 2022 by Hong Zhu, Wei Ding, Wenjuan Han, Xiaofan Zheng, Yiqing Hu, Jie Dong, Yaling Wu, Danxiao Wu, Jinhui Liu, Faming Zhu

“…The HIV antibody or antigen and HIV RNA were detected using enzyme-linked immunosorbent assay and NAT, respectively. …”
Get full text

Effectiveness of Voytik-Harbin Protocol in Fabrication of Ram’s Testicular-Derived Hydrogel and Its Impact on Mouse In Vitro Spermatogenesis by Leila Rashki Ghaleno, Tohid Rezaei Topraggaleh, Leila Montazeri, Abdolhossein Shahverdi, Mojtaba Rezazadeh Valojerdi

“…Followingmouse testicular cell culture, TDH and collagen hydrogel did not differ significantly in terms of cell survival ratesand the mRNA expression of early spermatogenesis genes.Conclusion: Using the VH protocol for producing ram TDH resulted in a firm hydrogel with a high frequency ofrepeat, which may be suited for testicular cell growth.…”
Get full text

Exploiting Cancer Dormancy Signaling Mechanisms in Epithelial Ovarian Cancer Through Spheroid and Organoid Analysis by Emily J. Tomas, Yudith Ramos Valdes, Jennifer Davis, Bart Kolendowski, Adrian Buensuceso, Gabriel E. DiMattia, Trevor G. Shepherd

“…Our results indicated that AMP-activated protein kinase (AMPK) activity was significantly upregulated and protein kinase B (Akt) was downregulated in EOC spheroids compared to organoids, suggesting a clear differential phenotype. Through RNA sequencing analysis, we further supported these phenotypic differences and highlighted the significance of cell cycle regulation in organoids. …”
Get full text

The Association of Mitochondrial tRNACys G5783A Mutation with Major Depressive Disorder in Two Han Chinese Families by Jing P, Yu H, Sun W, Liang M, Xia T, Yang H, Chen P, Li J, Zhang X

“…Thus, the phenotypic tRNACys G5783A mutation expression associated with MDD may be impacted by nuclear modifier gene(s) or environmental factors.Keywords: major depressive disorder, mitochondrial DNA, tRNA, mutation, haplogroup, Chinese…”
Get full text

The blood pressure lowering effect of beetroot juice is impaired in periodontitis and recovered after periodontal treatment by Nydia Y. Sanchez-Orozco, Bob T. Rosier, Alondra Ruiz-Gutierrez, Fabiola Marquez-Sandoval, Alejandro Artacho, Lucrecia Carrera-Quintanar, Alex Mira

“…Additionally, subgingival and tongue samples were taken to analyse the bacterial composition with Illumina sequencing of the 16S rRNA gene. In healthy individuals, the systolic and diastolic blood pressure (SBP and DPB) decreased significantly (both P < 0.01) 90 min after BRJ intake, but not in periodontitis patients. …”
Get full text

Adiponectin regulates proliferation and differentiation of chicken skeletal muscle satellite cells via ERK1/2 and p38 signaling pathways by Liping Guo, Kaiming Jin, Qi Sun, Chenchao Zhang, Xiongyong Chen, Zhaoyu Geng

“…Our results demonstrated that adiponectin promoted SMSCs proliferation while inhibiting myogenic differentiation and inducing adipogenic differentiation. RNA-seq analysis revealed enrichment of the MAPK signaling pathway, suggesting its potential involvement in the regulation of adiponectin on SMSCs activity. …”
Get full text

内质网应激参与蛋氨酸-胆碱缺乏饮食诱导的小鼠非酒精性脂肪肝的形成 by 方婉军, 凌文华

“…【结果】MCD 组 NAS 评分和血清中 ALT、AST 以及肝组织中 TG、TC 水平均高于普通膳食组；MCD 组 p- PERK、p-eIF2α、CHOP蛋白表达亦明显高于正常膳食组，而GADD34 mRNA表达显著低于普通膳食组，且随时间推移，MCD 组内质网应激逐渐加重；相关分析表明在MCD组中内质网应激相关指标与NAS评分呈显著相关。…”
Get full text

Identification of compounds against atherosclerosis induced by ox-LDL based on cell extraction/UPLC–MS/MS from mulberry twigs and their mechanistic analysis by Zhihui Jin, Wei Xiang, Xiaowen Shi, Fenglin Jiang, Yanan Jia, Yuansong Zhang, Lingshu Zeng, Xianzhi Huang, Li Xu

“…Real-time qPCR and Western blotting were used for mRNA and protein level analysis. Results Two novel active compounds, Kuwanon H and Morusin, and the known Kuwanon G, were identified. …”
Get full text

Variation in human gut microbiota impacts tamoxifen pharmacokinetics by Yasmine Alam, Sheron Hakopian, Lizett Ortiz de Ora, Ian Tamburini, Julio Avelar-Barragan, Sunhee Jung, Zane Long, Alina Chao, Katrine Whiteson, Cholsoon Jang, Elizabeth Bess

“…Here, using gnotobiotic- and antibiotics-treated mice, we show that tamoxifen pharmacokinetics are heavily influenced by gut bacteria and prolonged exposure to tamoxifen. Interestingly, 16S rRNA gene sequencing shows tamoxifen does not affect overall microbiota composition and abundance. …”
Get full text

Successful Diagnosis of Sengers Syndrome Using a Comprehensive Genomic Analysis by Kohta Nakamura, Yukiko Yatsuka, Sachie Naito, Akira Hasegawa, Takeya Kasukawa, Atsushi Kondo, Yoshihito Kishita, Yohei Sugiyama, Takanori Onuki, Tomohiro Ebihara, Tomoko Tsuruoka, Takuya Fushimi, Akira Ohtake, Kei Murayama, Atsuko Imai‐Okazaki, Yasushi Okazaki

“…Methods We employed a comprehensive genomic analysis approach, including whole‐genome sequencing and RNA sequencing, combined with various bioinformatics tools. …”
Get full text

The Toxoplasma rhoptry protein ROP55 is a major virulence factor that prevents lytic host cell death by Margarida T. Grilo Ruivo, Ji-hun Shin, Todd Lenz, Stephanie Y. Matsuno, Katherine Olivia Yanes, Arnault Graindorge, Maguy Hamie, Laurence Berry-Sterkers, Mathieu Gissot, Hiba El Hajj, Karine G. Le Roch, Melissa B. Lodoen, Maryse Lebrun, Diana Marcela Penarete-Vargas

“…We identified ROP55, a rhoptry protein that promotes parasite survival by preventing lytic cell death in absence of IFN-γ stimulation. RNA-Seq analysis revealed that ROP55 acts as a repressor of host pro-inflammatory responses. …”
Get full text

The transcription factor HMGB2 indirectly regulates APRIL expression and Gd-IgA1 production in patients with IgA nephropathy by Huijuan Tian, Yaling Zhai, Shuaigang Sun, Wenhui Zhang, Zhanzheng Zhao

“…In addition, we used DNA pull-down, protein profiling, and transcription factor prediction software to identify proteins bound to the promoter region of the APRIL gene. RNA interference and coimmunoprecipitation (Co-IP) were used to verify the relationships among HMGB2, high mobility group AT-hook protein 1 (HMGA1), and APRIL.Results HMGB2 expression was greater in IgAN patients than in HCs and was positively associated with APRIL expression in B cells. …”
Get full text

Weighted gene co-expression network analysis reveals key stromal prognostic markers in pancreatic cancer by G. Mantini, A. Agostini, M. Tufo, S. Rossi, M. Kulesko, C. Carbone, L. Salvatore, G. Tortora, G. Scambia, L. Giacò

“…Although extensive research with network analyses such as Weighted Gene Co-expression Network Analysis (WGCNA) has been conducted on pancreatic cancer and its stromal components, WGCNA has not previously been applied to isolate and identify genes associated with the abundance of stroma and survival outcome from bulk RNA data. We investigated the gene expression profile and clinical information of 140 pancreatic ductal adenocarcinoma patients from TCGA. …”
Get full text