Detection of Dengue Virus-Specific IgM and IgG Antibodies through Peptide Sequences of Envelope and NS1 Proteins for Serological Identification by Pradeep Kumar Nagar, Deepali Savargaonkar, Anupkumar R. Anvikar

“…Dengue is an acute febrile illness caused by positive-sense single-stranded RNA virus, belonging to the family Flaviviridae and genus Flavivirus. …”
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Molecular Epidemiology of SARS-CoV-2 within Accra Metropolis Postlockdown by Frank T. Aboagye, Lawrence Annison, Henry Kwadwo Hackman, Maame E. Acquah, Yvonne Ashong, Isaac Owusu-Frimpong, Bill C. Egyam, Sharon Annison, George Osei-Adjei, Samuel Antwi-Baffour

“…Samples were subjected to nucleic acid extraction and followed by real-time polymerase chain reaction for the detection and quantification of SARS-CoV-2 RNA. SARS-CoV-2 positive samples were subsequently subjected to variant identification using rapid PCR. …”
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Air-liquid interface culture alters the characteristics and functions of monolayers generated from human iPS cell‑derived enterocyte‑like cell organoids by Tatsuya Inui, Yusei Uraya, Yukiko Ueyama-Toba, Hiroyuki Mizuguchi

“…However, their activities were not enhanced. RNA-seq analysis suggested that ALI culture increased the expression of genes related to metabolic processes but decreased glycolytic processes. …”
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Molecular Epidemiology and Genetic Evolution of Porcine Reproductive and Respiratory Syndrome Virus in Northern China During 2021–2023 by Na Yuan, Zuofeng Yang, Fengxia Lv, Lina Dou, Xiangqing Li, Baokai Zhao, Shishan Dong

“…Porcine reproductive and respiratory syndrome virus (PRRSV), an important pathogen affecting the pig industry, is an RNA virus with high genetic diversity. In this study, 12,299 clinical samples were collected from northern China during 2021–2023 to investigate the molecular epidemiological characteristics and genetic evolution of PRRSV. …”
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Myocardial Expression of PPARγ and Exercise Capacity in Patients after Coronary Artery Bypass Surgery by Izabela Wojtkowska, Tomasz A. Bonda, Jadwiga Wolszakiewicz, Jerzy Osak, Andrzej Tysarowski, Katarzyna Seliga, Janusz A. Siedlecki, Maria M. Winnicka, Ryszard Piotrowicz, Janina Stępińska

“…The mean distance 1 month after CABG in HF was 397±85 m versus 420±93 m in non-HF. PPARγ mRNA expression was similar in both HF and non-HF groups. 6MWT distance 1 month after CABG was inversely correlated with PPARγ level only in HF group. …”
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Bacterial Composition of Dairy Base during Fermentation by Mariya V. Gryaznova, Inna Yu. Burakova, Yuliya D. Smirnova, Ekaterina Yu. Nesterova, Natalia S. Rodionova, Evgeniy S. Popov, Mikhail Yu. Syromyatnikov, Vasily N. Popov

“…To determine the microbiome of the substrates, the authors used the next-generation high-throughput sequencing that targeted V3 of 16S rRNA gene. The fermentation resulted in a decrease in Bifidobacterium and an increase in Lactobacillus, which peaked (97.5%) after 15 h of fermentation. …”
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Lingguizhugan Decoction Protects against High-Fat-Diet-Induced Nonalcoholic Fatty Liver Disease by Alleviating Oxidative Stress and Activating Cholesterol Secretion by Lili Yang, Weili Lin, Colleen A. Nugent, Shijun Hao, Haiyan Song, Tao Liu, Peiyong Zheng

“…Four weeks for the treatment, liver tissues were harvested for RNA sequencing. Differentially expressed genes (DEGs) and enriched pathways were detected on hepatic global transcriptome profile. …”
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Egfr Amplification Specific Gene Expression in Phyllodes Tumours of the Breast by Konstantin Agelopoulos, Christian Kersting, Eberhard Korsching, Hartmut Schmidt, Arno Kuijper, Christian August, Pia Wülfing, Joke Tio, Werner Boecker, Paul J. van Diest, Burkhard Brandt, Horst Buerger

“…Caveolin-1 and eps15, involved in EGFR turnover and signalling, were regulated differentially on the RNA and protein level proportionally to egfr gene dosage. …”
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CD154 Induces Interleukin-6 Secretion by Kidney Tubular Epithelial Cells under Hypoxic Conditions: Inhibition by Chloroquine by Antoine Dewitte, Julien Villeneuve, Sébastien Lepreux, Marion Bouchecareilh, Xavier Gauthereau, Claire Rigothier, Christian Combe, Alexandre Ouattara, Jean Ripoche

“…Both intracellular IL-6 expression and secretion were stimulated by CD154 and associated with a strong upregulation of IL-6 mRNA and increased transcription. Searching for inhibitors of CD154-mediated IL-6 production by HK-2 cells in hypoxic conditions, we observed that chloroquine, a drug that has been repurposed as an anti-inflammatory agent, alleviated this induction. …”
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Skeletal abnormalities caused by a Connexin43R239Q mutation in a mouse model for autosomal recessive craniometaphyseal dysplasia by Yasuyuki Fujii, Iichiro Okabe, Ayano Hatori, Shyam Kishor Sah, Jitendra Kanaujiya, Melanie Fisher, Rachael Norris, Mark Terasaki, Ernst J. Reichenberger, I-Ping Chen

“…Cx43 KI/KI mice display reduced osteocyte dendrites. RNA from Cx43 KI/KI femoral cortical bones show reduced expression levels of Sost, Tnf-α, IL-1β, Esr1, Esr2, and a lower Rankl/Opg ratio. …”
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上调ｍｉＲ－１００降低宫颈癌细胞侵袭和迁移及顺铂耐药性

“…【结果】 ①两系宫颈癌细胞上调ｍｉＲ－１００后，细胞的侵袭、迁移性降低；②上调ｍｉＲ－１００后的宫颈癌细胞中上皮相关分子Ｅ－Ｃａｄｈｅｒｉｎ表达上调，间质表型分子Ｓｎａｉｌ、Ｖｉｍｉｎｔｉｎ表达下调，ＭＭＰ－２表达无差异，ｕＰＡ表达下降；③不同浓度顺铂作用（Ｐ ＜ ０．００１）及是否转染ｍｉｒ－１００ ｓｉＲＮＡ（Ｐ ＜ ０．００１）对细胞存活率的改变均具有显著统计学意义，且浓度因素与处理因素间相对独立；在Ｓｉｈａ细胞中得出相同结论（Ｐ ＜ ０．００１）。…”
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Culture-based and Sanger sequencing approaches to uncover the diversity of leaf-fungal endophytes in neotropical gymnosperms by Omayra Meléndez, Rita Bethancourt, Ariadna Bethancourt, Lilisbeth Rodríguez–Castro, Jorge Mendieta, Armando A. Durant Archibold, Marta Vargas, Brian Sedio, Kristin Saltonstall, Juan Carlos Villarreal A.

“…Sequencing of 69 cultures with the ITS rRNA locus identified several ascomycetes: Colletotrichum, Cercophora, Hypoxylon, Phyllosticta, Xylaria, and a basidiomycete: Tritirachium. …”
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Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines by Jovanović Krivokuća Milica, Legner Janko, Ćujić Danica, Vilotić Aleksandra, Grubiša Ivana, Vićovac Ljiljana, Bojić-Trbojević Žanka

“…Expression levels of these galectins at both mRNA and protein levels were evaluated using PCR, qPCR, and Western blotting, while their cellular localization and distribution were assessed through immunocytochemistry, subcellular fractionation, and Western blotting. …”
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Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells by Fraser I. Young, Vsevolod Telezhkin, Sarah J. Youde, Martin S. Langley, Maria Stack, Paul J. Kemp, Rachel J. Waddington, Alastair J. Sloan, Bing Song

“…Importantly, only DPSC clones with high nestin mRNA expression levels were found to successfully differentiate into Map2 and NF-positive neuronal-like cells. …”
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<i>Myrica rubra</i> Preharvest Treatment with Melatonin Improves Antioxidant and Phenylpropanoid Pathways During Postharvest Storage by Jun-Quan Chen, Yun-Shuang Ma, Hejiang Zhou, Rui-Xue Yu, Miao Xiong, Na Yang, Ji-Qiu Wang, Yang Tian, Ling-Yan Su

“…Meanwhile, melatonin increased the content of antioxidants such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as the total polyphenols and flavonoids content. Finally, RNA transcriptome sequencing revealed that melatonin enhanced the antioxidant capacity by increasing the expression of both antioxidant enzymes and changing phenylpropanoid pathway-related genes, therefore maintaining the fresh <i>Myrica rubra</i> quality. …”
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Zellweger syndrome; identification of mutations in PEX19 and PEX26 gene in Saudi families by Abdulfatah M. Alayoubi, Ambreen Ijaz, Abdul Wali, Jamil A. Hashmi, Azizah Alharbi, Sulman Basit

“…Moreover, in this study, the importance of a synonymous exonic variant of PEX26 close to the splice donor site was explored in relation to pre-mRNA splicing and resulting disease manifestations.…”
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Assessment of immune responses and intestinal flora in BALB/c mice model of wheat food allergy via different sensitization methods by Chenglong Liu, Chen Chen, Xingyu Yan, Shimin Gu, Xin Jia, Wenhui Fu, Xiao Meng, Wentong Xue

“…The level of serum antibodies in intraperitoneal injection group was significantly higher than control. Subsequently, 16S rRNA sequencing technology was used to analyze the intestinal flora of mice. …”
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Preliminary Evidence of a Horizontal Transfer of Paramutation Phenomenon at the <i>pl1</i> Gene in Maize (<i>Zea mays</i> L.) by Roberto Pilu, Martina Ghidoli, Alessia Follador, Alessandro Passera, Paola Casati, Ervane Laure Cheyep Dinzeu, Elena Cassani, Michela Landoni

“…Despite extensive research, the molecular mechanisms underlying paramutation are still not fully understood, although numerous studies suggest the involvement of RNA-mediated changes in DNA methylation and in the chromatin structure of silenced genes. …”
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Efficacy and Safety of Sofosbuvir-Based Direct-Acting Antiviral Agents Treatment for Patients with Genotype 3/6 Hepatitis C Virus Infection by Yong-yu Mei, You-ming Chen, Yuan-kai Wu, Xiao-hong Zhang, Wen-xiong Xu

“…Age was 42.5 ± 10.4 years. Baseline HCV RNA was 6.29 ± 0.89log10 IU/mL. The numbers of GT 3a, 3b, and 6a patients were 10, 12, and 32, respectively. …”
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Analysis of toxicity and mechanisms of busulfan in non-obstructive azoospermia: A genomic and toxicological approach integrating molecular docking, single-cell sequencing, and expe... by Yanggang Hong, Qichao Yuan, Yi Wang, Deqi Wang, Xiaoju Guan, Congde Chen

“…By integrating molecular docking, single-cell RNA sequencing, and in vivo experimentation, the study identified POLE and LRAT as critical proteins. …”
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