Low regulatory T-cells frequency is associated with graft rejection after small bowel transplantation: Clinical and experimental evidence. by Rodrigo Papa-Gobbi, Pablo Stringa, Maria Virginia Gentilini, Ivana Ivanoff, Mariana Machuca, Nidia Monserrat Arreola, Javier Serradilla, Karla Estefanía-Fernández, Paloma Talayero, María Velayos, Elena Sánchez-Zapardiel, Gabriel Gondolesi, Ane Andrés-Moreno, Martin Rumbo, Francisco Hernández-Oliveros

“…Notably, ACR correlated with a high CD8/CD4 ratio, loss of T-cell chimerism, mRNA upregulation of pro-inflammatory genes and diminished graft Treg frequency. …”
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Lipopolysaccharide-Binding Protein Downregulates Fractalkine through Activation of p38 MAPK and NF-κB by Xia Huang, Yi Zeng, Yujie Jiang, Yueqiu Qin, Weigui Luo, Shulin Xiang, Suren R. Sooranna, Liao Pinhu

“…A549 cells were transfected with LBP or LBP shRNA plasmid DNA or pretreated with SB203580 or SC-514 following LPS treatment. …”
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Prevalence of nasopharyngeal bacteria during naturally occurring bovine respiratory disease in commercial stocker cattle by Afroza Akter, Hannah Teddleton, Marc Caldwell, Gina Pighetti, Phillip R. Myer, Madison T. Henniger, Liesel Schneider, Elizabeth Shepherd

“…After sequencing, 16S rRNA V4 gene was amplified and utilized for NP bacterial determination. …”
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Elevated POSTN expression predicts poor prognosis and is associated with radioresistance in cervical cancer patients treated with radical radiotherapy by Cui-qin Huang, Wen-tao Xiao, Xiang-rong Yao, Zhi-min Li, Jun-yan He

“…We analyzed data from 92 CC patients in The Cancer Genome Atlas (TCGA) and 153 patients from our institution, assessing POSTN expression levels through mRNA analysis and immunohistochemistry (IHC). Our findings revealed that high POSTN expression was significantly associated with advanced tumor stages, poorer radiotherapy outcomes, and worse overall survival (OS). …”
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Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer by Eloisa Vendemiatti, Rafael Oliveira Moreira, Gabriel Lasmar dos Reis, Inty Omar Hernandez-De Lira, Eugenia Peña-Yewtukhiw, Franz Walter Rieger Hippler, Luis Omar Torres-Dorante, Kiran Pavuluri, Alex Valentine, Vitor L. Nascimento, Vagner Augusto Benedito

“…The transcriptional response to B was more widespread compared to Zn. RNA-Seq of leaves under different B levels revealed modulated genes with potential roles in nutritional homeostasis and stress response that may be key to controlling B status in the plant. …”
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Association of Myeloid Cells of Triggering Receptor-1 with Left Ventricular Systolic Dysfunction in BALB/c Mice with Sepsis by Gaosheng Zhou, Lijun Ye, Liang Zhang, Liangqing Zhang, Yuanli Zhang, Liehua Deng, Huaguo Yao

“…Myocardium TNF-α (at 6 h and 24 h) and sTREM-1 (at 6 h) were significantly higher in LPS/mAbTrem-1-treated mice than in time-matched LPS-treated mice; meanwhile myocardium TNF-α mRNA were markedly increased in comparison with LPS-treated or saline-treated mice at 24 h. …”
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Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight by Jiang Nan, Qiu Jiehua, Tian Dagang, Shi Huanbin, Liu Zhiquan, Wen Hui, Xie Shuwei, Chen Huizhe, Wu Meng, Kou Yanjun

“…Analysis of 16S and internal transcribed spacer rRNA gene sequencing data revealed that the mixture of the T40 and T208 strains exhibited the lowest average clustering coefficients, which were negatively correlated with the biomass of F. oxysporum-inoculated rice seedlings. …”
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Macrophage P2X7 Receptor Function Is Reduced during Schistosomiasis: Putative Role of TGF-β1 by Suellen D’arc Santos Oliveira, Hayandra Ferreira Nanini, Luiz Eduardo Baggio Savio, Mariana Caldas Waghabi, Claudia Lucia Martins Silva, Robson Coutinho-Silva

“…Western blot and qRT-PCR data showed no difference in P2X7 protein and mRNA between uninfected, infected, and TGF-β1-treated groups. …”
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Role of nasal microbiota in regulating host anti-influenza immunity in dogs by Jinzhu Geng, Yuhao Dong, Hao Huang, Xia Wen, Ting Xu, Yanbing Zhao, Yongjie Liu

“…Considering that influenza A virus is increasingly recognized as an important canine respiratory pathogen, this study investigated the cross-talk between nasal and lung tissues mediated by microbes and its association with influenza susceptibility in a beagle dog model. Results Using 16S rRNA gene sequencing, combined with comparative transcriptomic, anatomical, and histological examinations, we investigated viral presence, gene expression profiles, and microbiota in the nasal cavity and lung after influenza infection in the beagles with antibiotic-induced nasal dysbiosis. …”
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Comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pi... by Xiuqing Yang, Meijie Liu, Yanjiao Zhang, Lizhong Guo, Junqi Wang, Sizhu Li, Yan Zhang, Xiaobo Li, Lili Xu, Hao Yu

“…The highly expressed proteins related to transcription, RNA splicing, the production of various organelles, DNA conformational change, nucleosome organization, protein processing, maturation and transport, and cell detoxification regulated the pileus development and maturity. …”
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In ovo delivery of carvacrol triggers expression of chemotactic factors, antimicrobial peptides and pro-inflammatory pathways in the yolk sac of broiler chicken embryos by Mila M. Y. Meijer, Henry van den Brand, Shahram Niknafs, Eugeni Roura

“…Yolk sac tissue samples were collected at E19.5, and transcriptomic analyses using RNA sequencing were performed, following functional enrichment analyses comparing the control (saline) and carvacrol-injected groups. …”
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Protective effects of Notoginsenoside R2 on reducing lipid accumulation and mitochondrial dysfunction in diabetic nephropathy through regulation of c-Src by Xieyi Guo, Liu Yang, Xiaoning An, Maofang Hu, Yilan Shen, Niansong Wang, Youhua Xu, Dingkun Gui

“…Lipid accumulation, oxidative stress levels, mitochondrial function and cell apoptosis were also assessed. Small interfering RNA was used in in vitro experiments to examine the effect of c-Src. …”
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Elucidating the expression and role of cGAS in pan-cancer using integrated bioinformatics and experimental approaches by Zhen Lian, Xue Liu, Xue Li

“…In this study, The Cancer Genome Atlas (TCGA) and Cancer Cell Line Encyclopedia (CCLE) data were used to analyze the mRNA expression and genomic alterations of cGAS in pan-cancer. …”
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Effects of orally administered clioquinol on the fecal microbiome of horses by Mikaila Z. Smith, Mary York, Kile S. Townsend, Lynn M. Martin, Tamara Gull, Lyndon M. Coghill, Aaron C. Ericsson, Philip J. Johnson

“…Feces were obtained daily for 7 days before, during, and after conclusion of treatment, and again 3 months later. Libraries of 16S rRNA V4 region amplicons generated from fecal DNA were sequenced using the Illumina sequencing platform. …”
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雷公藤甲素对哮喘小鼠气道重塑及 转化生长因子-β1表达的影响

“…测定支气管管壁厚度(WAt/Pbm),支气管平滑肌厚度(WAm/Pbm)?取右肺组织行RT-PCR检测TGF-β1 mRNA表达。【结果】 B组小鼠支气管管壁厚度、杯状细胞百分数、气道平滑肌厚度、胶原纤维面积、TGF-β1的转录和表达水平均显著高于A组(P < 0.01)。…”
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Blockade of ICAM-1 Improves the Outcome of Polymicrobial Sepsis via Modulating Neutrophil Migration and Reversing Immunosuppression by Yan-jun Zhao, Wen-jing Yi, Xiao-jian Wan, Jun Wang, Tian-zhu Tao, Jin-bao Li, Jia-feng Wang, Xiao-ming Deng

“…The results demonstrated that ICAM-1 mRNA was upregulated in lung, thymus, and spleen of CLP mice. …”
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Advancements in the Treatment of Mucopolysaccharidoses: From Established Therapies to Gene Therapy by Joanna Wanat, Wojciech Homa, Aleksandra Warunek, Gabriela Gronowicz, Izabela Dzikowska, Agata Siejka, Daria Stefaniak, Weronika Zielińska, Michał Chról

“…Among the promising new treatments are: blood-brain barrier (BBB)-penetrable enzyme replacement therapy (ERT), substrate reduction therapy, nonsense suppression therapy, and mRNA engineering. However, the most advanced treatments, that are currently under investigation are in-vivo and ex-vivo gene therapy. …”
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Importance of appropriate genome information for the design of mating type primers in black and yellow morel populations by Melissa Cravero, Aaron J. Robinson, Patrick Hilpisch, Patrick S. Chain, Saskia Bindschedler, Pilar Junier

“…The yellow morel primer pairs (EMAT1-1 L/R and EMAT1-2 L/R) appear to have reporting errors, as the published primer sequences are dissimilar with reported amplicon sequences and the EMAT1-2 primers appear to amplify the RNA polymerase II subunit (RPB2) gene. The lack of the reference genome used in primer design and descriptive methodology made it challenging to fully assess the apparent issues with the primers for this clade. …”
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A Highly Efficient System for Separating Glandular and Non-glandular Trichome of Cucumber Fruit for Transcriptomic and Metabolomic Analysis by Lei Sun, Zhongxuan Feng, Fang Wang, Yu Qi, Menghang An, Lin Yang, Min Feng, Mingqi Wang, Huazhong Ren, Xingwang Liu

“…The process begins with the pre-cooling of sorbitol buffer or ethanol solution and the RNA-free treatment of laboratory supplies, followed by sterilization and pre-cooling. …”
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A Novel Liposome Formulation Carrying Both an Insulin Peptide and a Ligand for Invariant Natural Killer T Cells Induces Accumulation of Regulatory T Cells to Islets in Nonobese Dia... by Hidetoshi Akimoto, Emi Fukuda-Kawaguchi, Omar Duramad, Yasuyuki Ishii, Kazunari Tanabe

“…Cotransfer of diabetogenic T cells and splenocytes of NOD mice treated with RGI-3100-iB, but not liposomal α-GalCer encapsulating an unrelated peptide, to NOD-SCID mice resulted in the prevention of diabetes and elevation of Foxp3 mRNA expression in the islets. These data indicate that the migration of insulin B-peptide-specific Tregs to islet of NOD mice that are involved in the suppression of pathogenic T cells related to diabetes onset and progression could be enhanced by the administration of liposomes containing α-GalCer and insulin B peptide.…”
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