A Cross-Sectional Trait Versus State Biomarker Analysis of Inflammatory Cytokines and miRNAs in Patients with Major Depressive Disorder by Christopher Rockson, Chandrashekaran Girish, Harivenkatesh Natarajan, Vikas Menon

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IL-21 Modulates Release of Proinflammatory Cytokines in LPS-Stimulated Macrophages through Distinct Signaling Pathways by Su-nan Li, Wei Wang, Shou-peng Fu, Jian-fa Wang, Hong-mei Liu, Shan-shan Xie, Bing-run Liu, Yang Li, Qing-kang Lv, Zhi-qiang Li, Wen-jing Xue, Bing-xu Huang, Wei Chen, Ju-xiong Liu

“…The results showed that IL-21 significantly inhibited LPS-induced mRNA expression of IL-1β, TNF-α, and IL-6 in macrophages, but not of IFN-γ, IL-10, CCL5, or CXCL2. …”
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Valorization of rice stubble through biodegradation using hydrolytic enzyme-producing Olivibacter oleidegradans CMB10 and Agrobacterium pusense SFMB9 by Milind Gajbhiye, Sushmita Patil, Sagar Awate, Supriya Kokare, Siddharth Terdale, Manish Kumar Dubey

“…They were phenotypically characterized concerning Bergey's Manual of Determinative Bacteriology followed by molecular characterization by 16S rRNA gene sequencing and CMB10 and SFMB9 were identified as Olivibacter oleidegradans and Agrobacterium pusense, respectively. …”
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Sigma-1 Receptor Modulates CFA-Induced Inflammatory Pain via Sodium Channels in Small DRG Neurons by Yuanlong Song, Zifen Xu, Liangpin Zhang, Linlin Gao

“…Our results revealed several key findings: (1) The expression of Sig-1R was found to be upregulated during the progression of inflammatory pain, with a notable translocation from the cytoplasm to the membrane; (2) Inhibition of peripheral Sig-1R using S1RA resulted in a reduction of CFA-induced allodynia; (3) Activation of Sig-1R through PRE-084 led to a decrease in the fast sodium current in isolated DRG neurons from CFA-treated rats, which was associated with a diminished action potential (AP) peak and maximum depolarizing rate (MDR), as well as an increased rheobase; (4) Furthermore, PRE-084 was observed to enhance the slow component of the sodium current, resulting in hyperpolarization of the threshold potential and an increase in AP firing frequency, alongside an elevation in the mRNA expression of the slow sodium channel Nav1.9 in CFA-treated rats. …”
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Effects of Synthetic Astaxanthin on the Growth Performance, Pigmentation, Antioxidant Capacity, and Immune Response in Black Tiger Prawn (Penaeus monodon) by Qiang Chen, Shuting Huang, Jieyu Dai, Congcong Wang, Songming Chen, Yuanxin Qian, Yangyang Gong, Tao Han

“…Correspondingly, astaxanthin decreased GSH and MDA contents under transportation stress. Moreover, the mRNA expression of immune genes (traf6, relish, and myd88) were inhibited by dietary astaxanthin supplementation. …”
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Unveiling the link between NADPH oxidase 2 activation and mitochondrial superoxide formation in leukemic cell killing induced by arsenic trioxide by Andrea Spina, Andrea Guidarelli, Gloria Buffi, Mara Fiorani, Orazio Cantoni

“….-) formation induced by clinically relevant concentrations of arsenic trioxide (ATO; As2O3) in acute promyelocytic leukemia (APL) cells.Carefully controlled inhibitor studies and small interfering RNA mediated downregulation of p47phox (a component of the NOX 2 complex) expression demonstrated that, in an APL cell line, ATO promotes upstream NOX 2 activation critically connected with the formation of mitoO2.- and with the ensuing mitochondrial permeability transition (MPT)-dependent apoptosis.Instead, acute myeloid leukemia (AML) cell lines respond to ATO with low NOX 2 activation, resulting in a state that is non-permissive for mitoO2.- formation. …”
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Adamantane Resistance in Seasonal Human Influenza A Viruses from Calgary, Alberta (January 2007 to August 2008) by Kanti Pabbaraju, Sallene Wong, Dmitri K Kits, Julie D Fox

“…The amplification and sequencing of regions that confer adamantane resistance directly from RNA extracts of clinical samples (without previous culture) makes this approach a fast and efficient process for monitoring resistance. …”
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Mother-fetus immunogenetic dialogue as a factor of progeny immune system development by L. A. Gerlinskaya, A. V. Varlachev, G. I. Krotov, G. V. Kontsevaya, M. P. Moshkin

“…Since breast milk contains large amounts of various immunoactive components, including proteins, cytokines, hormones, immunoglobulins, exosomes containing micro-RNA, and viable immune cells, the immunomodulating effects of breast milk persist even after elimination of maternal immunoglobulins from the blood of the offspring, up to maturation. …”
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Astragaloside IV alleviates GDM via regulating gut microbiota and gut microbiota metabolomic by Fengge Wang, Yongning Zhu, Hua Shu, Xiaoyun Zhang, Liting Duan, Dongmei Man, Yanping Wang

“…The measurements of relevant indicators pertaining to GDM symptoms and reproductive outcomes, along with the 16S rRNA sequencing data and LC-MS-based metabolomic profiles, were assessed across all groups.ResultsAfter the 25-day intervention, the GDM model + AS IV group showed significantly decreased fasting blood glucose levels (p = 0.0003), mean insulin levels (p = 0.0001), and insulin resistance index (p = 0.0001). …”
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High-dose thiamine supplementation ameliorates obesity induced by a high-fat and high-fructose diet in mice by reshaping gut microbiota by Yu Xia, Yu Xia, Lulu Wang, Yanyan Qiu, Weihong Ge, Weihong Ge, Weihong Ge

“…Biochemical analysis and histological analysis were conducted to assess phenotypic changes. Fecal 16S rRNA gene sequencing was performed to analyze alterations in the gut microbiota.ResultsThe results showed that high-dose thiamine supplementation for eight weeks could significantly alleviate symptoms of HFFD-induced obesity and improve HFFD-induced intestinal epithelial barrier dysfunction by enhancing the tight junction function. …”
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Effect of eicosapentaenoic acid on innate immune responses in Atlantic salmon cells infected with infectious salmon anemia virus by Ingrid Holmlund, Samira Ahmadi, Bente Ruyter, Tone-Kari Østbye, Marta Bou, Tor Gjøen

“…The study includes seven experimental groups: ± ISAV and various levels of EPA up to 200 µM. Analysis of RNA sequencing data showed that more than 3000 genes were affected by ISAV alone (without additional EPA). …”
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A molecular basis for spine color morphs in the sea urchin Lytechinus variegatus by Maria Wise, Madison Silvia, Gerardo Reyes, Rushane Dunn, Thomas M. Onorato, Cosmo Pieplow, Aidan Furze, El Hebert, Nathalie Oulhen, Dan Ritschoff, David R. McClay, Gary Wessel

“…The abundance of mRNAs encoding flavin mono-oxygenase variancts and polyketide synthase was predictive of the color of the adult and antibodies identified their histological location in the spine cells. By RNA in situ hybridization, candidate genes important for spine biomineralization and pigmentation were mapped onto the spine epithelia, and MicroCT scans of spines from different color morphs concluded that color morphs are entirely due to pigmentation and not to structural variations of the endoskeleton. …”
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RNAseq of peripheral blood mononucleated cells exposed to platelet-rich fibrin and enamel matrix derivatives by Layla Panahipour, Zahra Kargarpour, Michael Mildner, Hannes Kühtreiber, Reinhard Gruber

“…To this aim, we have exposed human peripheral blood mononucleated cells (PBMCs) to PRF lysates prepared by a swing-out rotor and EMD, followed by bulk RNA sequencing. A total of 111 and 8 genes are up- and down-regulated by PRF under the premise of an at least log2 two-fold change and a minus log10 significance level of two, respectively. …”
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De-biasing microbiome sequencing data: bacterial morphology-based correction of extraction bias and correlates of chimera formation by Luise Rauer, Amedeo De Tomassi, Christian L. Müller, Claudia Hülpüsch, Claudia Traidl-Hoffmann, Matthias Reiger, Avidan U. Neumann

“…Extracted DNA was sequenced (V1–V3 16S rRNA gene) together with corresponding DNA mocks. …”
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Detecting Fish Diversity in Urban‐Impacted Ecosystems: A Comparative Approach of eDNA Metabarcoding and UVC by Arthur Chung, Y. C. Kam, Stanley K. H. Shea, Celia Schunter

“…We employ and compare two methods: 12S rRNA eDNA metabarcoding coupled with custom built reference database and simultaneous extensive underwater visual census (UVC) surveys. eDNA detected a higher species richness per site. …”
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Escherichia coli in septic arthritis: prevalence and antibiotics susceptibility patterns by Masroora Ali Khan, Muhammad Kamran Taj, Zahid Khan, Imran Taj, Muhammad Naeem, Saima Azam

“…The polymerase chain reaction (PCR) based identification of E. coli showed clear bands of 204bp of the 16S rRNA gene. Sequence analysis using the Basic Local Alignment Search Tool found high similarity with pathogenic E. coli from Egypt and China. …”
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焦孔素D介导的滑膜血管内皮细胞焦亡促进类风湿关节炎关节破坏 by 吴滔, 张学培, 卢烨, 邹耀威, 欧阳志明, 马剑达, 戴冽

“…体外RA滑液干预人脐静脉血管内皮细胞（HUVEC）模拟炎症环境，检测细胞凋亡水平及焦亡通路相关蛋白表达。siRNA转染敲减GSDMD，检测HUVEC细胞焦亡、分泌细胞因子、增殖、迁移和血管生成能力的变化。…”
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Human Chorionic Plate-Derived Mesenchymal Stem Cells Restore Hepatic Lipid Metabolism in a Rat Model of Bile Duct Ligation by Yun Bin Lee, Jong Ho Choi, Eun Nam Kim, Jin Seok, Hyun-Jung Lee, Jung-Hwan Yoon, Gi Jin Kim

“…CPT1A expression was changed via microRNA-33—a posttranscriptional regulator of CPT1A—in a peroxisome proliferator-activated receptor α-independent manner. …”
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Perspectives on Structural, Physiological, Cellular, and Molecular Responses to Desiccation in Resurrection Plants by Yathisha Neeragunda Shivaraj, Plancot Barbara, Bruno Gugi, Maïté Vicré-Gibouin, Azeddine Driouich, Sharatchandra Ramasandra Govind, Akash Devaraja, Yogendra Kambalagere

“…Similarly, recent data show a major role of ABA, LEA proteins, and small regulatory RNA in regulating DT responses. Current progress in “-omic” technologies has enabled quantitative monitoring of the plethora of biological molecules in a high throughput routine, making it possible to compare their levels between desiccation-sensitive and DT species. …”
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Gene Expression Pattern and Regulatory Network of α-Toxin Treatment in Bombyx mori by Tieshan Feng, Ping Lin, Jiao Gong, Dong Cheng, Xi Yang, Quan Zhang, Tingcai Cheng

“…In this study, we investigated the immune response of silkworm induced by α-toxin by using RNA-seq. We compared the changes in gene expression in the midgut, fatbody, and hemocytes of silkworm and in the B. mori embryonic cell line (BmE) after treatment with α-toxin and identified 952 differentially expressed genes and 353 differentially expressed long noncoding RNAs (lncRNAs). …”
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