The Expression and Clinical Significance of Different Forms of Mer Receptor Tyrosine Kinase in Systemic Lupus Erythematosus by Huaqun Zhu, Xiaolin Sun, Lei Zhu, Fanlei Hu, Lianjie Shi, Zhanguo Li, Yin Su

“…Real-time quantitative PCR was applied to evaluate the mRNA levels of MerTK and ADAM17. Results. Both mMer expression on CD14+ monocytes/macrophages and sMer levels in plasma significantly increased in SLE patients compared to healthy subjects. …”
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Intratumor heterogeneity of HPV integration in HPV-associated head and neck cancer by Noah Sasa, Toshihiro Kishikawa, Masashi Mori, Rie Ito, Yumie Mizoro, Masami Suzuki, Hirotaka Eguchi, Hidenori Tanaka, Takahito Fukusumi, Motoyuki Suzuki, Yukinori Takenaka, Keisuke Nimura, Yukinori Okada, Hidenori Inohara

“…Heterozygous loss of ataxia-telangiectasia mutated (ATM) was observed in 67% of tumors, with its downregulation confirmed by single-cell RNA sequencing and immunohistochemistry, suggesting ATM haploinsufficiency contributes to carcinogenesis. …”
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CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches. by Wiam El Kheir, Sahar Naasri, Bernard Marcos, Nick Virgilio, Benoit Paquette, Nathalie Faucheux, Marc-Antoine Lauzon

“…Semi-quantitative qPCR showed higher CXCR4 mRNA levels in F98 cells than in U87 cells. CXCL12 significantly increased intracellular calcium levels via CXCR4 activation, with a 2.3-fold rise in F98 cells compared to U87, consistent with observed cell behavior during perfusion. …”
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Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats by Ali Y. Naoom, Wenyi Kang, Nora F. Ghanem, Mohamed M. Abdel-Daim, Fatma M. El-Demerdash

“…Furthermore, histological, immunohistochemical alpha-smooth muscle actin immunoreactivity (α-SMA-ir) and histochemical (collagen, total protein, DNA, and RNA) alterations were observed in rat kidneys. Moreover, STZ produced upregulation of inflammatory associated genes (tumor necrosis factor-alpha; TNF-α and transforming growth factor β1; TGF-β1) and triggered apoptosis by upregulating apoptotic related gene [Bcl2-associated X protein (Bax)] and downregulating anti-apoptotic related gene B-cell lymphoma-2 (Bcl-2) based on real-time PCR data. …”
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Molecular Mechanism During Mycelium Subculture Degeneration of <i>Volvariella volvacea</i> by Lidan Feng, Lujuan Wang, Yuanxi Lei, Jie Li, Fengyun Zhao

“…Thus, five generations of subcultured strains (M1–M5) were obtained after 20 months of mycelial subculturing, their production traits were determined, and transcriptomic analysis was performed using RNA-seq; the differentially expressed genes were verified via RT-qPCR. …”
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Bacillus velezensis A-27 as a potential biocontrol agent against Meloidogyne incognita and effects on rhizosphere communities of celery in field by Yanan Yao, Liyi Wang, Haozhen Zhai, Hailong Dong, Jianming Wang, Zengqi Zhao, Yumei Xu

“…In this study, strain A-27 was identified as Bacillus velezensis based on its morphological, physiological, and molecular characteristics, including 16 S rRNA and gyrA sequencing. Strain A-27 exhibited high larvicidal and ovicidal efficacy in vitro, with estimated LC50 values of 4.0570 × 108 CFU/mL for larvicidal efficacy and 3.6464 × 108 CFU/mL for ovicidal efficacy. …”
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Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida by Eveline-Marie Lammens, Daniel C. Volke, Alison Kerremans, Yannick Aerts, Maarten Boon, Pablo I. Nikel, Rob Lavigne

“…Abstract The T7 phage RNA polymerase (RNAP) is a widely used expression platform, but its implementation in non-model microbial hosts poses significant challenges due to cytotoxicity. …”
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Comparative transcriptomic and phenotypic analysis of monoclonal and polyclonal Populus deltoides genotypes by Macy Gosselaar, Macy Gosselaar, Mark A. Arick, Chuan-Yu Hsu, Heidi Renninger, Courtney M. Siegert, Waqar Shafqat, Daniel G. Peterson, Austin Himes

“…Phenotypic results showed that polyclonal plots exhibited higher leaf area index (LAI; p &lt; 0.01, 2.96 ± 0.057 m2) and total biomass (p &lt; 0.01, 2.74 ± 0.06) compared to monoclonal plots, indicating superior productivity. RNA sequencing revealed upregulation of key genes such as exocyst subunit exo70 family protein H7 (EXO70H7), NDH-dependent cyclic electron flow 5 (NDF5), and expansin-like A3 (EXLA3). …”
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Bivalirudin exerts antiviral activity against respiratory syncytial virus-induced lung infections in neonatal mice by Zhuang Shihao, Tang Qiuyu, Chen Ping, Wang Chengyi, Liu Guanghua

“…Furthermore, RSV-infected mice demonstrated altered clotting parameters such as D-dimer, soluble thrombomodulin, and increased inflammatory cytokines IL-5, 6, IFN-γ, IL-13, and CXCL1. Additionally, the mRNA expression analysis displayed increased levels of IL-33, TLR3, and TLR7 genes in RSV-infected lung tissue. …”
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Liposomal Formulations: A Recent Update by Surendra S. Agrawal, Vrinda Baliga, Vaishali Y. Londhe

“…Advanced drug delivery technologies like magnetic targeting and ultrasound. Pro Drug, RNA Liposomes approach may improve liposomal medication administration. …”
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INFORMACINIS GENOMAS, INDIVIDO IR POPULIACIJOS GENOMAI by Jonas Rubikas

“…Information genome is the DNA or RNA molecule carrying information for the beginning of replication (plasmid), for the replication of enzymes and other proteins and structures connected with the replication (viruses). …”
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Antiviral Drugs and Vaccines for Omicron Variant: A Focused Review by Nidhi Garg, Ananya Sree Kunamneni, Pankaj Garg, Sandeep Sharma, Divakar Sharma, Adinarayana Kunamneni

“…Regarding vaccine efficacy (VE), the 3-dose VE with mRNA vaccines at 14–60 days was found to be 71.6%, and after 60 days, it is 47.4%. …”
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TFE3-rearranged perivascular epithelioid cell tumors of the head and neck with rare fusion partners: clues to the differential diagnosis between benign and malignant tumors by Yuka Takahashi, Akihiko Yoshida, Seiichi Yoshimoto, Shigenobu Suzuki, Satsuki Kishikawa, Ayaka Mitsui, Eijitsu Ryo, Yuki Kojima, Kan Yonemori, Yasushi Yatabe, Taisuke Mori

“…However, a subsequent renal tumor biopsy revealed angiomyolipoma. The RNA sequence revealed ZC3H4::TFE3 and PRCC::TFE3 fusions in the first and second cases, respectively. …”
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Analytical and diagnostic performance characteristics of reverse-transcriptase loop-mediated isothermal amplification assays for dengue virus serotypes 1-4: A scoping review to inf... by Paul Arkell, Dumrong Mairiang, Adisak Songjaeng, Kenny Malpartida-Cardenas, Kerri Hill-Cawthorne, Panisadee Avirutnan, Pantelis Georgiou, Alison Holmes, Jesus Rodriguez-Manzano

“…Assays' lower limit of detection (LLOD) and linear range of quantification were comparable to RT-qPCR (with lowest reported values 2.2x101 and 1.98x102 copies/ml, respectively, for studies which quantified target RNA copies) and analytical specificity was high. …”
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Dietary Cocoa Powder Improves Hyperlipidemia and Reduces Atherosclerosis in apoE Deficient Mice through the Inhibition of Hepatic Endoplasmic Reticulum Stress by Hua Guan, Yan Lin, Liang Bai, Yingfeng An, Jianan Shang, Zhao Wang, Sihai Zhao, Jianglin Fan, Enqi Liu

“…The groups fed dietary cocoa powder showed a significant reduction in both plasma cholesterol levels and aortic atherosclerosis compared to the control group. Analysis of mRNA profiling of aortic atherosclerotic lesions revealed that the expression of several genes related to apoptosis, lipid metabolism, and inflammation was significantly reduced, while the antiapoptotic gene Bcl2 was significantly increased in the cocoa powder group compared to the control. …”
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Distinct gut microbial signature and altered short chain fatty acid metabolism at disease onset in a rat preclinical model of superimposed preeclampsia by Moumen M. Alhasan, María S. Landa, Silvia I. García, Roman G. Gerlach, Hani Harb, Fabian B. Fahlbusch, Melanie L. Conrad, Gabriela Barrientos

“…On gestation day (GD)14, feces were collected and gut microbiome composition and short-chain fatty acid concentrations were determined by 16S rRNA sequencing and gas chromatography respectively. …”
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Circ-ADAM9 Promotes High Glucose-Induced Retinal Pigment Epithelial Cell Injury in DR via Regulating miR-338-3p/CARM1 Axis by Zeyu Liang, Chengzhe Lu, Tingting Feng, Xiang Gao, Yongfang Tu, Wenchao Yang, Yipeng Wang

“…The expression of circ-ADAM9, microRNA (miR)-338-3p, and coactivator-associated arginine methyltransferase 1 (CARM1) was measured using quantitative real-time PCR. …”
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脂代谢相关基因ABHD5在泛癌中的表达模式及其与预后和免疫浸润的相关性分析 by 陈宇涛, 杨洋, 夏淦, 许淼泓, 曹霁昭, 卢嘉茵, 邹焱, 杨霞

“…方法在本研究中，使用GTEx、TCGA、TIMER2.0、CPTAC数据库及免疫组织化学法、免疫印迹法探究ABHD5在泛癌中的表达模式及其与不同临床分期的相关性，使用Kaplan-Meier Plotter、Oncolnc、R2数据库探究ABHD5与预后的相关性，并使用TCGA、TIMER2.0数据库探究ABHD5与免疫细胞浸润之间的相关性，再用STRING、GEPIA数据库获取肿瘤中与ABHD5结合或共表达的基因，进行GO和KEGG富集分析。结果与正常组织相比，ABHD5在多种肿瘤中mRNA和蛋白表达水平下调，在少数肿瘤中升高。ABHD5高表达在8种肿瘤中与较好的预后相关，在2种肿瘤中与较差的预后相关，在9种肿瘤中与免疫细胞浸润水平呈正相关关系，并在泛癌中与中性粒细胞浸润及免疫检查点基因的表达具有显著正相关性。…”
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RNAi-based ALOX15B silencing augments keratinocyte inflammation in vitro via EGFR/STAT1/JAK1 signalling by Megan A. Palmer, Rebecca Kirchhoff, Claudia Buerger, Yvonne Benatzy, Nils Helge Schebb, Bernhard Brüne

“…Using a cytokine cocktail containing IL-17A, interferon-gamma and tumour necrosis factor-alpha to produce a psoriasis-like phenotype, a role for ALOX15B in human epidermal keratinocyte inflammation was investigated. siRNA-mediated silencing of ALOX15B increased CCL2 expression and secretion. …”
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INFORMACINIS GENOMAS, INDIVIDO IR POPULIACIJOS GENOMAI by Jonas Rubikas

“…Information genome is the DNA or RNA molecule carrying information for the beginning of replication (plasmid), for the replication of enzymes and other proteins and structures connected with the replication (viruses). …”
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