小檗碱对甲型流感病毒FM1株感染小鼠肺中RLH信号通路的影响

“…与病毒组相比，RIG-I、NF-κB和MAVS 的mRNA表达量分别降低42.28%、33.12%和48.12%，同时，它们的蛋白表达量分别降低17.37%、13.83%和28.91%。 …”
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Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity by Mervat G. Hassan, Gharieb S. El-Sayyad, Mohamed O. Abdel-Monem, Mohamed N. Malash, Mona A. Kishk, Mohamed E. El Awady, Mohamed I. El-khonezy

“…Streptomyces sp. strain 5 M was identified by examining the 16S rRNA gene in the promising strain G8. A pH of 7.5, an incubation temperature of 40 °C, and the use of glucose and peptone as the carbon and nitrogen sources, respectively, produced the highest quantities of L-glutaminase. …”
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Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model by Samah Fouad, Awad Rizk, Esam Mosbah, Mostafa M. Nabeeh, Walaa Awadin, Ayman S. Elmezayyen, Ekramy Elmorsy, Adel Zaghloul

“…After a 30-days follow-up, macroscopic examination, histological studies, and mRNA evaluation for TGFβ-1and IL-6, were conducted. …”
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Integrated bioinformatics and experimental analysis of CHAF1B as a novel biomarker and immunotherapy target in LUAD by Wei Du, Xiao-Wei Wu, Qing-Feng Li, Bing-Yu Zhang, Jing Wu, Ya-Ping Xu, Xue Yi

“…We also predicted lncRNAs-miRNA-CHAF1B axis to explore the molecular mechanism of CHAF1B in LUAD. …”
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Repression of CADM1 transcription by HPV type 18 is mediated by three-dimensional rearrangement of promoter-enhancer interactions. by Karen Campos-León, Jack Ferguson, Thomas Günther, C David Wood, Steven W Wingett, Selin Pekel, Christy S Varghese, Leanne S Jones, Joanne D Stockton, Csilla Várnai, Michelle J West, Andrew Beggs, Adam Grundhoff, Boris Noyvert, Sally Roberts, Joanna L Parish

“…The virus-induced changes to the host cell's transcriptome are thought to contribute to carcinogenesis. Here, we show by RNA-sequencing that oncogenic HPV18 episome replication in primary human foreskin keratinocytes (HFKs) drives host transcriptional changes that are consistent between multiple HFK donors. …”
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Salivary microbiota dysbiosis and elevated polyamine levels contribute to the severity of periodontal disease by Md Haroon Rashid, Sandhya Pavan Kumar, Resma Rajan, Anitha Mamillapalli

“…Microbiota analysis was carried out by 16S rRNA sequencing on the Nanopore PromethIONsystem platform of Oxford Nanopore technologies. …”
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Computational Study Protocol: Leveraging Synthetic Data to Validate a Benchmark Study for Differential Abundance Tests for 16S Microbiome Sequencing Data [version 2; peer review: 2... by Clemens Kreutz, Eva Kohnert

“…Building on Nearing et al.’s study (1), who assessed 14 differential abundance tests using 38 experimental 16S rRNA datasets in a case-control design, we are generating synthetic datasets that mimic the experimental data to verify their findings. …”
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NT5E (CD73) as a prognostic biomarker and therapeutic target associated with immune infiltration in lung adenocarcinoma by Leyan Chen, Tuoya Qi, Bishu Zhang, Xuelong Wang, Mingfeng Zheng

“…We found that NT5E was significantly overexpressed at both the mRNA and protein levels in LUAD tissues. Elevated NT5E expression was significantly linked to multiple clinicopathological factors, including metastasis and pathological stage, and served as a strong predictor of poor prognosis in LUAD patients. …”
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Pediatric pancreatic acinar cell carcinoma with a non-canonical BRAF-KMT2C fusion and a classic SND1-BRAF fusion: a case report and literature review by Yaqin Wang, Jiasi Zhang, Dimin Nie, Ai Zhang, Qun Hu, Aiguo Liu

“…Comprehensive genomic profiling by next-generation sequencing identified a non-canonical BRAF fusion with KMT2C at the DNA level and a classic SND1-BRAF fusion at the RNA level. The patient underwent surgical resection through a Whipple operation followed by six cycles of mFOLIRINOX chemotherapy and radiation therapy, achieving a favorable outcome up to now. …”
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Astrocytic Acid-Sensing Ion Channel 1a Contributes to the Development of Epileptic Cognitive Impairment by Wen Li, Huimin Zhou, Xiaona Li, Gengyao Hu, Dong Wei

“…Status epilepticus (SE) in C57/BL6 mice was induced using lithium–pilocarpine; the impact of ASIC1a on astrocytes was assessed using rAAV–ASIC1a–NC and rAAV–ASIC1a–shRNA, injected in the CA3 region of mice. Behavioral assessments were conducted using the Morris water maze (MWM). …”
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Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use by J. M. J. Van den Bergh, E. L. J. M. Smits, M. Versteven, H. De Reu, Z. N. Berneman, V. F. I. Van Tendeloo, E. Lion

“…In this study, we demonstrate that upon electroporation with both IL-15 and IL-15Rα-encoding messenger RNA, mature DC become highly positive for surface IL-15, without influencing the expression of prototypic mature DC markers and with preservation of their cytokine-producing capacity and their migratory profile. …”
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Crosstalk between non-coding RNAs and programmed cell death in colorectal cancer: implications for targeted therapy by Seyed Reza Taha, Mehdi Karimi, Bahar Mahdavi, Milad Yousefi Tehrani, Ali Bemani, Shahriar Kabirian, Javad Mohammadi, Sina Jabbari, Meysam Hushmand, Alireza Mokhtar, Mohammad Hossein Pourhanifeh

“…The targeted modulation of ncRNA-PCD interactions presents promising avenues for personalized cancer treatment, which may improve patient outcomes by enhancing therapeutic effectiveness and reducing resistance.…”
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Characteristics of gut microbiota profiles in Asian elephants (Elephas maximus) with gastrointestinal disorders by Sarisa Klinhom, Chanon Kunasol, Sirawit Sriwichaiin, Sasiwan Kerdphoo, Nipon Chattipakorn, Siriporn C. Chattipakorn, Chatchote Thitaram

“…The samples were subjected to DNA extraction and amplification targeting the V3-V4 region of 16S rRNA gene for next-generation sequencing analysis. …”
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Characterization of Vaccine-Enhanced Humoral Immune Responses Against Emergent SARS-CoV-2 Variants in a Convalescent Cohort by Jared Sheehan, Amber J. Trauth, Michael E. Hagensee, Alistair J. Ramsay

“…Our primary goal was to characterize hybrid antibody (Ab) responses in a patient cohort infected with ancestral Wuhan-Hu-1 virus and vaccinated between 6 and 10 months later with the Wuhan-Hu-1–based BNT162b2 mRNA vaccine. We were particularly interested in determining the efficacy of neutralizing Ab responses against subsequently emergent SARS-CoV-2 variants. …”
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Silica Nanoparticles Loaded With Selenium Quantum Dots Reduce Myocardial Ischemia-Reperfusion Injury by Alleviating Ferroptosis and Mitochondrial Dysfunction by Li T, Yang B, Liu X, Shi D, Wang Z, Chen Y, Shen C

“…Taixi Li,1,* Boshen Yang,1,* Xijian Liu,2 Dongmei Shi,1 Zhixiang Wang,1 Yizhi Chen,1 Chengxing Shen1 1Department of Cardiology, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, People’s Republic of China; 2School of Chemistry and Chemical Engineering, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Noncoding RNA, Shanghai Engineering Technology Research Center for Pharmaceutical Intelligent Equipment, Shanghai University of Engineering Science, Shanghai, 201620, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chengxing Shen, Department of Cardiology, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, People’s Republic of China, Email shencx@sjtu.edu.cnPurpose: Myocardial ischemia-reperfusion (IR) injury, a significant challenge in cardiovascular treatment, is primarily driven by ferroptosis and mitochondrial dysfunction. …”
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缺氧诱导因子-1在兔结膜伤口愈合模型的表达 by 龚若文, 吴克玲, 左成果, 黄丹平, 林明楷

“…【结果】Western Blot结果显示HIF-1α蛋白表达水平在术后第3天（P＜0.001）和第5天（P＜0.001）均高于空白对照组。在术后不 同时间点，HIF-1α的mRNA水平的变化趋势与HIF-1α的蛋白水平的变化趋势相一致。术后结膜血管化水平与 HIF-1α的蛋白水平呈正相关关系（r=0.626；P＜0.001）。…”
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A Novel Pathogenic Splicing Mutation of OFD1 is Responsible for a Boy with Joubert Syndrome Exhibiting Orofaciodigital Spectrum Anomalies, Polydactyly and Retinitis Pigmentosa by Chen L, Zhao MF, Deng HW, Liao M, Fan LL, Zhong QB, Wang J, Li K, Wu ZH, Yin JY

“…Whole exome sequencing and Sanger sequencing revealed a splicing mutation (NM_003611.2, c.2387+1G>A) in the OFD1 gene of the patient and his mother. mRNA sequencing further confirmed this mutation. …”
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Limosilactobacillus mucosae FZJTZ26M3 prevents NAFLD in mice through modulation of lipid metabolism and gut microbiota dysbiosis by Danting Dang, Bowen Li, Mengfan Ding, R. Paul Ross, Catherine Stanton, Jianxin Zhao, Bo Yang, Wei Chen

“…Furthermore, the results of 16S rRNA gene sequencing revealed a drop in the relative abundance of Ruminococcaceae, which is linked to inflammation, but the relative abundance of a potential probiotic Akkermansia significantly increased after L. mucosae FZJTZ26M3 intervention. …”
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OPTIMIZATION OF CONCENTRATION AND SOAKING TIME OF HARRIS HEMATOXYLIN IN DNA BAND EXAMINATION USING ELECTROPHORESIS by Siti Nuristiqomah Fajri, Asep Iin Nur Indra, Fusvita Merdekawati, Acep Tantan H.

“…The amplified DNA I6S rRNA gene from Escherichia coli (584 bp), which had undergone electrophoresis, was stained using harris hematoxylin dye at 0.01%; 0.02%; 0.03%; 0.04%; and 0.05% concentrations and immersion times soaking times of 10, 15, 20, and 30 minutes variations. …”
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Antimicrobial Resistance of Escherichia coli Isolated from Ground Beef in Huasca de Ocampo, Hidalgo, Mexico by A. P. Cordero-López, V. Vega-Sánchez, V. M. Martínez-Juárez, J. I. Olave-Leyva, F. R. Gómez-De Anda, N. E. Reyes-Rodríguez

“…The isolated strains were identified by traditional means and molecular by the 16S rRNA gene, the antibiotic sensitivity profile was identified by the Kirby-Bauer method and genotypic identification was performed for the type 1 integrase gene. …”
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