CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1 by Kamilla Wiull, Lisa K. Haugen, Vincent G. H. Eijsink, Geir Mathiesen

“…Importantly, the plasmid carrying the SgRNA, Cas9, and homology-directed repair template is designed for easy component exchange. …”
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Expansion of Circulating T Follicular Helper Cells in Children with Acute Henoch-Schönlein Purpura by Jue Xie, Yan Liu, Lei Wang, Guoxiang Ruan, Huiming Yuan, Hong Fang, Jianyong Wu, Dawei Cui

“…A positive correlation was observed between the frequencies of circulating ICOS+CXCR5+CD4+TFH cells and the serum IL-21 or IgA levels of acute HSP children, respectively. Additionally, the mRNA expression levels of interleukin- (IL-) 21, IL-6, and transcriptional factors (B-cell lymphoma-6, Bcl-6) were also significantly increased in peripheral blood from acute HSP children compared to HCs. …”
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Current molecular understanding of central nervous system schwannomas by Takahiro Tsuchiya, Satoru Miyawaki, Yu Teranishi, Kenta Ohara, Yudai Hirano, Shotaro Ogawa, Seiei Torazawa, Yu Sakai, Hiroki Hongo, Hideaki Ono, Nobuhito Saito

“…In addition to genetic alterations, research is also being conducted on gene expression and epigenetic regulation, with a focus on NF2 methylation and post-transcriptional silencing by micro RNA. Furthermore, the advent of advanced techniques like single-cell sequencing and multi-omics analysis has facilitated rapid discoveries related to the tumor microenvironment and tumor heterogeneity in schwannomas. …”
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Macrophage STING signaling promotes fibrosis in benign airway stenosis via an IL6-STAT3 pathway by YiLin Chen, ChengCheng Yang, YuShan Miao, DongChen Shi, Xiang Li, Sen Tian, YiFei Zhang, ChengFei Xu, YuChao Dong, ChaoFeng Han, Hui Shi, Chong Bai

“…Here we demonstrate using scRNA sequencing that cGAS‒STING signalling is involved in BAS, which is accompanied by increased dsDNA, expression and activation of STING. …”
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The study of organelle DNA variability in alloplasmic barley lines in the NGS era by M. G. Siniauskaya, A. M. Makarevich, I. M. Goloenko, V. S. Pankratov, A. D. Liaudanski, N. G. Danilenko, N. V. Lukhanina, A. M. Shimkevich, O. G. Davydenko

“…Some of the SNPs detected are very similar in codon position and in the type of amino acid substitution to the places where RNA editing can occur. Thus, these results outline new perspectives for the future study of nuclear-cytoplasmic interactions in alloplasmic lines.…”
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N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells by Yanli Liu, Fen Yang, Shengying Liang, Qing Liu, Sulei Fu, Zhenyu Wang, Ciqing Yang, Juntang Lin

“…Moreover, upregulation of N-cadherin (N-cad) mRNA and protein expression was observed after neurogenic differentiation. …”
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Association of Serum Myeloid Cells of Soluble Triggering Receptor-1 Level with Myocardial Dysfunction in Patients with Severe Sepsis by Fei Tao, Liangshan Peng, Juan Li, Yiming Shao, Liehua Deng, Huaguo Yao

“…There is no difference in TREM-1-mRNA expression between the two groups. Conclusions. …”
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Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer by V. G. Druzhinin, E. D. Baranova, P. S. Demenkov, L. V. Matskova, A. V. Larionov

“…In the present study, we determined the taxonomic composition of the sputum microbiome of 52 patients with squamous cell carcinoma of the lung, 52 patients with lung adenocarcinoma and 52 healthy control donors, using next-generation sequencing (NGS) on the V3-V4 region of the bacterial gene encoding 16S rRNA. The sputum microbiomes of patients with different histological types of lung cancer and controls did not show significant differences in terms of the species richness index (Shannon); however, the patients differed from the controls in terms of evenness index (Pielou). …”
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Impact of Scleroderma‐Associated Autoantibodies on Clinical Outcome Assessments: Post Hoc Analysis From a Randomised, Double‐blind, Placebo‐controlled, Phase 3 Trial of Tocilizumab... by Basmah Al Dulaijan, Suiyuan Huang, Celia J. F. Lin, Christopher P. Denton, Dinesh Khanna

“…We analyzed four groups with SSc‐Abs: anti–topoisomerase 1 antibody (ATA), anti–RNA polymerase 3 antibody (RNAP3), anti‐centromere antibody, and negative for all three (triple negative). …”
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Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota by Kai Kang, Yue Sun, Dan Pan, Bing Chang, Li-Xuan Sang

“…Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment. Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment. …”
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Allicin Alleviates Inflammation of Trinitrobenzenesulfonic Acid-Induced Rats and Suppresses P38 and JNK Pathways in Caco-2 Cells by Chen Li, Weijian Lun, Xinmei Zhao, Shan Lei, Yandong Guo, Jiayi Ma, Fachao Zhi

“…Allicin reduced the body weight loss of trinitrobenzenesulfonic acid-induced rats, histological score, serum TNF-α and IL-1β levels, and colon IL-1β mRNA level and induced serum IL-4 level, particularly in combination with mesalazine. …”
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Distinct roles of Constitutive Photomorphogenesis Protein 1 homolog (COP1) in human hepatocyte models by Sébastien Soubeyrand, Paulina Lau, Ruth McPherson, Ruth McPherson

“…HNF4A function could be restored by introducing a siRNA-resistant COP1 transgene, whereas HNF4A restoration partially rescued COP1 silencing in HepG2 cells. …”
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UHRF1 promotes epithelial-mesenchymal transition mediating renal fibrosis by activating the TGF-β/SMAD signaling pathway by Lijie Yang, Penghui Si, Tuoheti Kuerban, Linfa Guo, Shanzhi Zhan, Yisha Zuhaer, Yingtong Zuo, Peixiang Lu, Xiaojie Bai, Tongzu Liu

“…Hinokitiol has been reported to reduce the expression of UHRF1 mRNA and protein. We observed that inhibition of UHRF1 with Hinokitiol ameliorated induced EMT and renal fibrosis by reducing SMAD2/3 phosphorylation in vivo and in vitro. …”
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Combination Therapy with Platelet-Rich Plasma and Epidermal Neural Crest Stem Cells Increases Treatment Efficacy in Vascular Dementia by Somayeh Akbari, Masoud Haghani, Mojtaba Ghobadi, Etrat Hooshmandi, Afshin Borhani Haghighi, Mohammad Saied Salehi, Sareh Pandamooz, Negar Azarpira, Afsoon Afshari, Shahrbanoo Zabihi, Marzieh Nemati, Mahnaz Bayat

“…The basal-synaptic transmission (BST) and long-term potentiation (LTP) were assessed through field-potential recordings of the CA1. The mRNA expression levels of IGF-1, TGF-β1, PSD-95, and GSk-3β were measured in the rat hippocampus by quantitative reverse transcription polymerase chain reaction. …”
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Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats by ZHANG Naiqun, YUAN Piaopiao, CAO Linrong, YING Na, YANG Taotao

“…Objective This study aims to monitor the mRNA ratio of podocin to nephrin (PNR) in glomerular podocytes of early diabetic kidney disease (DKD) rat models. …”
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Elucidating the role of pyrimidine metabolism in prostate cancer and its therapeutic implications by Liang Huang, Yu Xie, Shusuan Jiang, Kan Liu, Zhihao Ming, Hong Shan

“…Through transcriptomic and single-cell RNA sequencing analyses, we explored metabolic pathway enrichment, immune infiltration patterns, and differential gene expression in prostate cancer samples. …”
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Negative Effects of High Glucose Exposure in Human Gonadotropin-Releasing Hormone Neurons by Annamaria Morelli, Paolo Comeglio, Erica Sarchielli, Ilaria Cellai, Linda Vignozzi, Gabriella B. Vannelli, Mario Maggi

“…Consistent with previous studies, leptin treatment significantly induced GnRH mRNA expression at 5 mM glucose, but not in the presence of high glucose concentrations. …”
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Insulin Increases Sestrin 2 Content by Reducing Its Degradation through the PI3K/mTOR Signaling Pathway by Dandan Chai, Guoyu Wang, Ziyu Zhou, Hanyan Yang, Zhiwen Yu

“…We then explored the potential feedback role of SESN2 in insulin signaling by SESN2 siRNA knockdown in HepG2 cells. Following SESN2 knockdown insulin-stimulated PKB phosphorylation was enhanced and accompanied by reduced PTEN content. …”
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Identification of new reference genes with stable expression patterns for cell cycle experiments in human leukemia cell lines by Otília Tóth, Gergely Attila Rácz, Eszter Oláh, Máté Tóth, Edit Szabó, György Várady, Beáta G. Vértessy, Nikolett Nagy

“…However, for reliable evaluation of qPCR data reference genes with stable mRNA expression are required. Although several studies investigating cell cycle-dependent gene expression employ frequently used reference genes, the suitability of these reference genes has not been thoroughly investigated so far. …”
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Simultaneously delivery of functional gallium ions and hydrogen sulfide to endow potentiated treatment efficacy in chemo- and PARPi-resistant ovarian cancer by Sangsang Tang, Yangyang Li, Yifeng Fang, Mengyan Tu, Shenglong Wu, Yixuan Cen, Junfen Xu

“…Furthermore, Ga2S3-BSA NMs increased cellular lipid peroxidation and triggered ferroptosis. RNA-seq analysis further clarified the comprehensive antitumor mechanisms of Ga2S3-BSA NMs. …”
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