Comparison of mucosal microbiota populations across the gastrointestinal tract of healthy dogs by Ching-Yen Lin, Tzu-Wen L. Cross, Kelly S. Swanson

“…DNA was extracted and the hypervariable region 4 (V4) of the 16S rRNA gene was amplified to assess microbiota using Illumina MiSeq sequencing. …”
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Comprehensive guide for epigenetics and transcriptomics data quality control by Arianna Comendul, Frederique Ruf-Zamojski, Colby T. Ford, Pankaj Agarwal, Elena Zaslavsky, German Nudelman, Manoj Hariharan, Aliza Rubenstein, Hanna Pincas, Venugopalan D. Nair, Adam M. Michaleas, Philip D. Fremont-Smith, Darrell O. Ricke, Stuart C. Sealfon, Christopher W. Woods, Kajal T. Claypool, Rafael Jaimes, III

“…Improved signature discovery, including the identification of the agent and timing of exposure, has been enabled by advancements in assaying techniques to detect RNA expression, DNA base modifications, histone modifications, and chromatin accessibility. …”
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Construction and characterization of an infectious cDNA clone of turtle grass virus X from a naturally infected Thalassia testudinum plant by Luis Alvarado-Marchena, Bradley T. Furman, Mya Breitbart

“…The complete genome of this positive-sense single-stranded RNA virus was amplified from field samples of Thalassia testudinum and assembled into a pLX-based mini binary vector using a multi-fragment directional cloning strategy, resulting in the infectious clone pLX-TGVX. …”
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Mir-615-5p inhibits cervical cancer progression by targeting TMIGD2 by Yan Zhao, Haitao Chen, Wenhui Zhang, Wei Shang, Jinwei Cao, Huijuan Zhao, Zhixiang Zou

“…Materials and methods The levels of miR-615-5p and TMIGD2 mRNA in tissue samples and cells were quantified through quantitative reverse transcription real-time PCR, followed by statistical analyses to investigate the correlation between miR-615-5p and clinical data. …”
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Gender-specific antibiotic-resistance patterns and characterization of bacterial flora of undergraduate students’ mobile phones and associated factors at a Nigerian university by Morufat Oluwatosin Olaitan, Akeem Ganiyu Rabiu, Zara Babagana Umara

“…Bacterial isolates (n = 6/21) that resisted antibiotics belonging to ≥ 3 different classes regarded as multidrug resistant (MDR) were subjected to 16 S rRNA sequencing and identified using the Basic Local Alignment Search Tool (BLAST) algorithm. …”
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Causal Mediation Analyses for the Natural Course of Hepatitis C: A Prospective Cohort Study by Yi-Ting Huang, Yao-Chun Hsu, Hwai-I Yang, Mei-Hsuan Lee, Tai-Hsuan Lai, Chien-Jen Chen, Yen-Tsung Huang

“…HCV infection was profiled by antibodies against HCV and HCV RNA in participants’ serum samples. The cohort data were linked to Taiwan’s National Health Insurance Research Database to determine the incidences of potential mediating diseases and mortality. …”
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Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model by Yang Liu, Chen Chen, Jinbo Liu, Zhenqiang Sun, Bo Shao, Yanxin Xu, Pengyuan Dang, Shengyun Hu, Haifeng Sun, Chaoguan Wang, Junhong Hu

“…The mechanism underlying the effect of tumor-derived exosomes on M2 macrophage polarization remains elusive.Results The novel circular RNA circPOLQ exhibited significantly higher expression in CRC tissues than in paired normal tissues. …”
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Bacteria-Inspired Synthesis of Silver-Doped Zinc Oxide Nanocomposites: A Novel Synergistic Approach in Controlling Biofilm and Quorum-Sensing-Regulated Virulence Factors in <i>Pseu... by Abirami Karthikeyan, Manoj Kumar Thirugnanasambantham, Fazlurrahman Khan, Arun Kumar Mani

“…The potent isolated <i>Bacillus cereus</i> SASAK, identified via 16S rRNA sequencing and deposited in GenBank under accession number MH885570, facilitated the microbial-mediated synthesis of ZnO nanoparticles and silver-doped ZnO NCs. …”
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Sodium Lactate Accelerates M2 Macrophage Polarization and Improves Cardiac Function after Myocardial Infarction in Mice by Jialiang Zhang, Fangyang Huang, Li Chen, Guoyong Li, Wenhua Lei, Jiahao Zhao, Yanbiao Liao, Yijian Li, Changming Li, Mao Chen

“…Flow-cytometric analysis results reported that sodium lactate repressed the number of the IL-6+, IL-12+, and TNF-α+ macrophages among LPS-stimulated bone marrow-derived macrophages (BMDMs) and increased the mRNA levels of Arg-1, YM1, TGF-β, and IL-10. Mechanistic studies revealed that sodium lactate enhanced the expression of P-STAT3. …”
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Metagenomic characterization of oral microbiome signatures to predict upper gastrointestinal and pancreaticobiliary cancers: a case–control study by Sujin Oh, Jaihwan Kim, Cheol Min Shin, Hyo-Jung Lee, Hye Seung Lee, Kyoung Un Park

“…Methods Saliva samples from cancer patients and age- and sex-matched healthy controls were analyzed using 16S rRNA-targeted sequencing, followed by comprehensive bioinformatics analysis. …”
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Profile of intestinal fungal microbiota in acute pancreatitis patients and healthy individuals by Meng-Qi Zhao, Miao-Yan Fan, Meng-Yan Cui, Su-Min Chen, Jing-Jing Wang, Ying-Ying Lu, Qiao-Li Jiang

“…Methods In this study, we sequenced ITS rRNA gene amplicons and examined the intestinal fungal microbiota in feces from 11 AP patients (the test group) and 15 healthy people (the control group). …”
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Prosaposin – A plausible biomarker in head and neck squamous cell carcinoma by Avradeep Datta, Rajandeep Kaur, Anshika Chauhan, Geeta Singh Boora, Himanshu Garg, Debajyoti Chatterjee, Jaimanti Bakshi, Sushmita Ghoshal, Arnab Pal

“…Effects of downregulation on the tumour hallmarks were analysed by down-regulating PSAP by shRNA in HNSCC cell lines. Results: Serum PSAP levels of cases at baseline (median: 2.736 ng/mL) were found to be significantly elevated when compared to healthy controls (median: 2.129 ng/mL) (p = 0.0297*). …”
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Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi by A. A. Kryukov, A. O. Gorbunova, E. M. Machs, Y. V. Mikhaylova, A. V. Rodionov, P. M. Zhurbenko, A. P. Yurkov

“…In our work, we have successfully used universal primers ITS3 and ITS4 for the sequencing in Illumina MiSeq of the 5.8S rDNA – ITS2 region of the 35S rRNA genes, which contain both a conservative and variable regions. …”
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Antipsychotic Drugs Reverse MK801-Inhibited Cell Migration and F-actin Condensation by Modulating the Rho Signaling Pathway in B35 Cells by Yi-Chyan Chen, Fu-Ming Tsai, Mao-Liang Chen

“…MK801-induced psychotic symptoms and also the Ras homolog family member A (RhoA) expression and cell division control protein 42 (cdc42) mRNA modulation in the rat brain have been investigated. …”
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Faecalibacterium prausnitzii regulates carbohydrate metabolic functions of the gut microbiome in C57BL/6 mice by Peiling Geng, Ni Zhao, Yufan Zhou, Reuben S. Harris, Yong Ge

“…Multiomic approaches were used to profile the impact of UT1 on the gut microbiome and associated metabolic activity of C57BL/6 mice at homeostasis. Both 16S rRNA and metagenomic sequencing demonstrated that oral administration of UT1 resulted in profound microbial compositional changes including a significant enrichment of Lactobacillus, Bifidobacterium, and Turicibacter. …”
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Peptidoglycan-Like Components in Z-100, Extracted from Mycobacterium tuberculosis Strain Aoyama B, Increase IL-12p40 via NOD2 by Takayuki Horii, Yuki Orikawa, Yuta Ohira, Runa Eta, Uiko Tominaga, Takanori Sato, Takao Tanaka

“…We found that Z-100 contained peptidoglycan-like components. In addition, an siRNA study showed that Z-100 increased the production of IL-12p40 via nucleotide-binding oligomerization domain 2 (NOD2). …”
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miR-145 Alleviates Smooth Muscle Cell Phenotype Transition via ADAM17-Mediated ACE2 Shedding by Juan Wen, Baiyi Tang, Lan Guo, Wei Chen, Xiaohong Tang

“…Cotreatment with ADAM17 siRNA partially reversed the inducible effect of miR-145 inhibitor on the EREG and MMP2, induced Ang-(1–7) excretion, and upregulated ACE2 and Mas receptor expression. …”
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Vibrio alginolyticus is the pathogen of “Baotou” disease causing serious damage to Gracilariopsis lemaneiformis cultivation in China by Tong Pang, Feng Wang, Qunqun Guo, Mengjie Zhang, Yuanyuan Sun, Jianguo Liu

“…Interestingly, Vibrio alginolyticus, which once reported to be a probiotic or pathogen for multiple marine organisms, was strongly proved to be the pathogen causing “Baotou” disease in this study. Analysis of 16S rRNA gene profiling revealed that V. alginolyticus was the most abundant and dominant bacterium on the algal thalli suffering from “Baotou” disease, whereas its presence was scarcely detected on healthy thalli. …”
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内质网应激通过抑制PI3K/AKT/mTOR信号通路诱导多形性胶质母细胞瘤干细胞凋亡的机制

“…A2B5的表达;caspase3/7细胞凋亡检测试剂盒检测衣霉素(Tun)和毒胡萝卜素(Thap)对GSC的凋亡作用及MTS法检测细胞增殖活性;同时Western blot分析凋亡蛋白caspase3,ER stress标志蛋白C/EBP 同源性蛋白(CHOP)及PI3K/AKT/mTOR信号通路标志蛋白AKT?mTOR表达水平;使用siRNA沉默CHOP基因后,检测上述蛋白表达水平变化?【结果】 1周后肿瘤干细胞球形成,并可以表达CD133?…”
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Shionone Inhibits Glomerular Fibirosis by Suppressing NLRP3 Related Inflammasome though SESN2-NRF2/HO-1 Pathway by Tian Xiao, Hanzhen Zhao, Yucong Wang, Mengyin Chen, Cong Wang, Chen Qiao

“…We also preformed experimentation on SESN2 deficiency models (SESN2 knockout mice and SESN2 siRNA in cells) to further prove our hypothesis. Results The results demonstrated that SH effectively suppressed glomerular fibrosis, induced adenosine monophosphate-activated protein kinase (AMPK) phosphorylation, and inhibited NLR family pyrin domain containing 3 (NLRP3) activation. …”
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