Overexpression of StBBX14 Enhances Cold Tolerance in Potato by Heng Zhang, Mingjun Chen, Xiaobo Luo, Li Song, Fei Li

“…RT-qPCR analysis showed that the expression patterns of eight DEGs were consistent between the qPCR and RNA-seq. These findings illustrate that the <i>StBBX14</i> played an important role in cold stress in potato and provided a data basis for the genetic improvement of cold resistance traits of potato.…”
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Probiotic Potential of Lactobacillus Strains with Antifungal Activity Isolated from Animal Manure by Soundharrajan Ilavenil, Hyung Soo Park, Mayakrishnan Vijayakumar, Mariadhas Valan Arasu, Da Hye Kim, Sivanesan Ravikumar, Ki Choon Choi

“…Biochemical and physiology properties of strains confirmed that the strains are related to the Lactobacillus sp.; further, the 16S rRNA sequencing confirmed 99.99% sequence similarity towards Lactobacillus plantarum. …”
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Time-Restricted Feeding Reinforces Gut Rhythmicity by Restoring Rhythms in Intestinal Metabolism in a Jetlag Mouse ModelSummary by Hui Leng, Theo Thijs, Louis Desmet, Guillaume Vanotti, Mona Farhadipour, Inge Depoortere

“…An around-the-clock bulk-RNA sequencing study was performed on the jejunal mucosa. …”
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Molecular and morphology identification of Squat lobster (Family Galatheidae) in Seribu Islands, Jakarta by Amanda Siregar Raissha, Putri Anggraini Nurlita, Restiana Endang Gelis Ester, Suryo Kusumo Bagus, Subhan Beginer, P Zamani Neviaty, Madduppa Hawis, Najwa Khalilah

“…The mitochondrial 16S rRNA gene target was used for molecular identification. …”
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Iron Reduces M1 Macrophage Polarization in RAW264.7 Macrophages Associated with Inhibition of STAT1 by Zhen-Shun Gan, Qian-Qian Wang, Jia-Hui Li, Xu-Liang Wang, Yi-Zhen Wang, Hua-Hua Du

“…Iron significantly reduced mRNA levels of IL-6, IL-1β, TNF-α, and iNOS produced by IFN-γ-polarized M1 macrophages. …”
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Effects of Pristane Alone or Combined with Chloroquine on Macrophage Activation, Oxidative Stress, and Th1/Th2 Skewness by Qiufang Ouyang, Ziyang Huang, Zhenhua Wang, Xiaoqing Chen, Jingqin Ni, Ling Lin

“…And the expression of T-bet/GATA-3 and IL-12/IL-10 mRNA in spleen were analyzed by real-time PCR. We found that pristane treatment for a period of 12 or 24 weeks triggered macrophage activation syndrome, characterized by hemophagocytosis in spleen and peripheral blood, enhanced lipid phagocytosis by peritoneal macrophages in vitro, erythropenia and leucopenia, increased anti-Smith, lactic dehydrogenase, triglyceride, and ferritin, as well as hypercytokinemia of IFN-γ, TNF-α, IL-4, and IL-6. …”
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Disruption of Gut Microbiota and Associated Fecal Metabolites in Collagen-Induced Arthritis Mice During the Early Stage by Wu D, Yin M, Cao D, Zhang X, Zhu Y, Wei Y, Li Y, Wen C, Zhou J

“…Footpad thickness, serum autoantibodies, and joint histopathology were used to assess the progression of RA. A combination of 16S rRNA sequencing, untargeted metabolomics and targeted short-chain fatty acids (SCFAs) analysis were employed to comprehensively investigate the alterations of gut microbiota and fecal metabolites in CIA during the pre-RA stage.Results: 20 days after the initial collagen immunization, CIA mice showed immune responses without joint symptoms, alongside gut microbiota disruption. …”
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Protocol to study the impact of breast cancer on colonization resistance of mouse microbiota using network node manipulation by Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Lourdes Mateos-Hernandez, Lianet Abuin-Denis, Apolline Maitre, Janet Avellanet, Arlem García, Dasha Fuentes, Alejandro Cabezas-Cruz

“…Here, we describe the procedures for tumor cell production and inoculation and 16S rRNA data processing. We then detail steps for constructing co-occurrence networks based on correlations between microbial abundances.For complete details on the use and execution of this protocol, please refer to Wu-Chuang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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The multi-target mechanism of action of Selaginella doederleinii Hieron in the treatment of nasopharyngeal carcinoma: a network pharmacology and multi-omics analysis by Huaguo Liang, Caifu Fang, Meng Qiu

“…Kaplan–Meier analysis and immune infiltration analysis further showed that these key genes are closely related to the prognosis and immune microenvironment of NPC patients. Single-cell RNA sequencing analysis revealed the expression distribution of key genes across different immune cell types and explored their roles in the differentiation process of malignant cells through pseudotime trajectory analysis. …”
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Activating CD137 Signaling Promotes Sprouting Angiogenesis via Increased VEGFA Secretion and the VEGFR2/Akt/eNOS Pathway by Bo Li, Yue Zhang, Runting Yin, Wei Zhong, Rui Chen, Jinchuan Yan

“…Inhibition of VEGFR2 by siRNA or XL184 (cabozantinib) and inhibition of downstream signaling by LY294002 (inhibits AKT activation) and L-NAME (eNOS inhibitor) remarkably abolished proangiogenic effects of CD137 signaling both in vitro and ex vivo. …”
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Traffic-related-air-pollutant PM2.5 Caused Toxicity on Caenorhabditis elegans with Cotreatment of High-dose Glucose and Tempeh by Jian-He Lu, Ming-Hsien Tsai, Sen-Ting Huang, Jia-De Lee, Ta-Chih Hsiao, Wan Nurdiyana Wan Mansor, How-Ran Chao

“…In addition, tempeh treatment also ameliorated the altered mRNA expression of the antioxidant gene in C. elegans treated with or without TRAP PM2.5 and with or without high-dose glucose diets. …”
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Connexin 37 Regulates the Kv1.3 Pathway and Promotes the Development of Atherosclerosis by Minqi Liao, Lihua Chen, Jiongbin Lu, Guangzhu Liang, Yongzhao Yao, Shumin Ouyang, Yanhua Yang, Zhengwei Jian, Suxia Guo

“…The indexes of body weight, blood glucose, and blood lipid of mice were collected. The protein and mRNA expression levels of Cx37 and Kv1.3 were detected by reverse transcription-PCR (RT-PCR), Western blot, and immunofluorescence technique. …”
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Investigation of Scutellaria Barbata’s immunological mechanism against thyroid cancer using network pharmacology and experimental validation by Gen Ouyang, Yuangui Zhu, Zhehao Ouyang

“…Protein results indicated that quercetin downregulated SPP1 mRNA and protein expression. This study combines database predictions with experimental validation to reveal the potential mechanisms of SBH against TC, providing effective strategies for the immunotherapy of TC.…”
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Antiaging and Antioxidant Bioactivities of Asteraceae Plant Fractions on the Cellular Functions of the Yeast Schizosaccharomyces pombe by Rika Indri Astuti, Muhammad Eka Prastya, Irmanida Batubara, Eka Budiarti, Aulia Ilmiyawati

“…Interestingly, S. nodiflora water fractions significantly upregulated the mRNA transcripts of the Pap1-mediated core environmental stress response, namely, ctt1 gene in S. pombe. …”
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Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice by Yangwenjie Wang, Yang Xiang, Ruiqi Wang, Xiangning Li, Jianxiong Wang, Siwang Yu, Ying Zhang

“…Importantly, SFN treatment improved antioxidative capacity of skeletal muscle in response to the acute exhaustive exercise by increasing mRNA and nucleoprotein expression of Nrf2 and these genes involved in antioxidant generation and decreasing blood creatine kinase (CK) and 4-hydroxy-2-nonenal (4-HNE)-modified protein levels in the HIIT mice.…”
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The Role of Yersinia enterocolitica O:3 Lipopolysaccharide in Collagen-Induced Arthritis by Katarzyna Kasperkiewicz, Anna S. Świerzko, Marta Przybyła, Janusz Szemraj, Jarosław Barski, Mikael Skurnik, Andrzej Kałużyński, Maciej Cedzyński

“…On the other hand, it seemed to be more hepatotoxic when compared with the other tested endotoxins, while the enlargement of inguinal lymph nodes and drop in hepatic MBL-C expression (at the mRNA level) were independent of LPS chemotype. Our data did not suggest no greater impact Y. enterocolitica O:3 on the development or severity of arthropathy related to anticollagen antibody-induced arthritis in mice, although its interaction with MBL-C and subsequent complement activation may contribute to some adverse effects.…”
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Molecular characterization and potential therapeutic roles of miR125a in HER-2 positive gastric cancer by Afraa Mamoori, Zena Hasan Sahib, Haider Alkafaji

“…In addition, the level of expression of this miRNA is correlated to different pathological and clinical parameters. …”
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Influenza B virus infection alters the regenerative potential of murine alveolar type 2 pneumocytes by Satoko Nakano, Cait E. Hamele, Aleksandra Tata, Purushothama Rao Tata, Nicholas S. Heaton

“…Here, the authors report that during an influenza B virus infection, some of the actively infected AT2 pneumocytes can ultimately eliminate all traces of the viral RNA and persist in the host long term. As a consequence of having been infected, however, the cells display an altered transcriptional profile and decreased proliferative capacity. …”
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miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells by Zhong-Yan Chen, Fei Chen, Nan Cao, Zhi-Wen Zhou, Huang-Tian Yang

“…Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs). With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. …”
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Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α4 by Jong Ho Choi, Yun Bin Lee, Jieun Jung, Seong Gyu Hwang, IL-Hoan Oh, Gi Jin Kim

“…Knockdown of integrin α4 by integrin α4 siRNA (siITGA4) treatment increased BM-MSC migration by upregulation of ROCK1, Rac1/2/3, and matrix metalloproteinase-2 regardless of oxygen tension. …”
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