Muribaculum intestinale-derived 3-hydroxybutyric acid from Heterophyllin B attenuated pulmonary fibrosis through IDO1-mediated ferroptosis by Ce Chen, Jialin Wang, Mengqin Cheng, Haifeng Xie, Wei Li, Chaofeng Zhang

“…Subsequently, the microbiota-dependent antifibrotic efficacy of HB was verified using various delivery routes, antibiotic treatments, and faecal microbiota transplantation. Functionally, 16S rRNA sequencing, untargeted metabolomics, and co-incubation experiments revealed that the antifibrotic efficacy of HB was primarily contingent on the enrichment of Muribaculum intestinale and its metabolite, 3-hydroxybutyric acid. …”
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The Critical Role of YAP/BMP/ID1 Axis on Simulated Microgravity‐Induced Neural Tube Defects in Human Brain Organoids by Di Guo, Bin Yao, Wen‐Wei Shao, Jia‐Chen Zuo, Zhe‐Han Chang, Jian‐Xin Shi, Nan Hu, Shuang‐Qing Bao, Meng‐Meng Chen, Xiu Fan, Xiao‐Hong Li

“…Furthermore, sing‐cell RNA sequencing data show that SMG results in perturbations in the number and function of neural stem and progenitor cell subpopulations. …”
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Differences in gut microbial diversity and composition between growth phenotypes of farmed juvenile sandfish, Holothuria scabra by Joselito A. Tabardillo, Marie Antonette Juinio-Meñez, Adam M. Reitzel, Rachel Ravago-Gotanco

“…To address this gap, this study used 16 S rRNA amplicon sequencing to characterize differences in gut and fecal microbiota among large and small juveniles reared in floating ocean nurseries. …”
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Identification and Validation of the Pyroptosis-Related Hub Gene Signature and the Associated Regulation Axis in Diabetic Keratopathy by Yi Cui, Li Wang, Wentao Liang, Li Huang, Shuting Zhuang, Hong Shi, Nuo Xu, Jianzhang Hu

“…A competing endogenous RNA network comprising 16 DEPRGs, 22 lncRNAs, 13 miRNAs, and 3 circRNAs was constructed. …”
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Transcript Changes of Placental Tissue in Gestational Diabetes Mellitus Based on Transcriptome Sequencing by Sun Y, Wang Y, Liu C, Yang J, Li Q, Zhao F

“…KEGG pathway enrichment analysis indicated the involvement of DEGs in signalling pathways related to PI3K-Akt signaling pathway and ECM-receptor interaction. qRT-PCR validation of five randomly selected DEGs confirmed consistent expression trends with RNA-Seq quantification.Conclusion: YWHAB, LEP, CCL21, PAPPA2, and SFN may be potential biological markers for the diagnosis of GDM, involved in the occurrence and development of GDM, and have certain value for disease prediction and diagnosis.Keywords: gestational diabetes, transcriptome sequencing, qRT-PCR validation, placental tissue, pathogenic genes…”
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Estimating the Effectiveness of Pfizer and Sinopharm Vaccines and their Relationship to Occupation, Residence, Smoking, and Body Mass Index During Pregnancy Smoking by Shahad Th. Jameel, Asmaa Haseeb Hwaid, Abdulrazak SH. Hasan

“…The study concluded that the overall protective rate of SARS-CoV-2 vaccines among participants in Diyala Governorate after twelve to seventeen months of vaccination was good, and that the Pfizer mRNA vaccine had a better outcome compared to the inactivated Sinopharm vaccine. …”
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Ribosome pausing in amylase producing Bacillus subtilis during long fermentation by Yaozu Han, Biwen Wang, Alberto Agnolin, Gaurav Dugar, Frans van der Kloet, Christopher Sauer, Paul Igor Costea, Max Fabian Felle, Mathis Appelbaum, Leendert W. Hamoen

“…Since these sites can neither be predicted based on codon specificity nor on secondary protein structures, we speculate that secondary mRNA structures are responsible for these translation pausing sites. …”
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Blastocystis and Giardia duodenalis infection in a male prison in Spain by Carla Muñoz-Antoli, Jacklyn Comas, María José Irisarri-Gutiérrez, Lucrecia Acosta, José Guillermo Esteban, Rafael Toledo

“…Positive samples were processed by PCR amplification of a 600-bp fragment of the Blastocystis SSU rRNA gene and partial sequences of the Giardia duodenalis bg genes. …”
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Caffeic Acid Phenethyl Ester Regulates PPAR’s Levels in Stem Cells-Derived Adipocytes by Luca Vanella, Daniele Tibullo, Justyna Godos, Francesca Romana Pluchinotta, Claudia Di Giacomo, Valeria Sorrenti, Rosaria Acquaviva, Alessandra Russo, Giovanni Li Volti, Ignazio Barbagallo

“…Additionally, exposure of ASC to high glucose levels decreased adiponectin and increased proinflammatory cytokines mRNA levels, which were reversed by Cape-mediated increase of insulin sensitivity. …”
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The Development of Methods of BLOTCHIP^®-MS for Peptidome: Small Samples in Tuberous Sclerosis by Kunio Yui, George Imataka, Kotaro Yuge, Hitomi Sasaki, Tadashi Shiohama, Kyoichi Asada, Hidehisa Tachiki

“…Therefore, ASD may develop due to TSC2 deficiency. TSC2 regulates microRNA biogenesis and Microprocessor activity via GSK3β. …”
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Partially hydrolyzed guar gum attenuates symptoms and modulates the gut microbiota in a model of SARS-CoV-2 infection by Jiayue Yang, Isaiah Song, Misa Saito, Tenagy Hartanto, Takeshi Ichinohe, Shinji Fukuda

“…Another non-infected set of hamsters was used for the analysis of the gut microbiome composition with 16S rRNA amplicon sequencing, serum, and faecal metabolites with GC–MS and LC–MS. …”
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Ginsenoside Rg1 Improves Differentiation by Inhibiting Senescence of Human Bone Marrow Mesenchymal Stem Cell via GSK-3β and β-Catenin by Ziling Wang, Rong Jiang, Lu Wang, Xiongbin Chen, Yue Xiang, Linbo Chen, Minghe Xiao, Li Ling, Yaping Wang

“…RT-qPCR is used to detect mRNA expression levels. Results. Rg1 regulates the differentiation of hBM-MSCs. …”
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Transcriptomic Analysis Reveals an Altered Hcy Metabolism in the Stria Vascularis of the Pendred Syndrome Mouse Model by Wenyue Xue, Yuxin Tian, Yuanping Xiong, Feng Liu, Yanmei Feng, Zhengnong Chen, Dongzhen Yu, Shankai Yin

“…Materials and Methods. RNA sequencing was applied to analyze the differential gene expression of the stria vascularis (SV) from wildtype and Slc26a4-/- mice. …”
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15-Deoxy-Δ12,14-prostaglandin J2 Induces Apoptosis and Upregulates SOCS3 in Human Thyroid Cancer Cells by Carlos Antônio Trindade-da-Silva, Carolina Fernandes Reis, Lara Vecchi, Marcelo Henrique Napimoga, Marcelo Sperandio, Bruna França Matias Colombo, Patrícia Terra Alves, Laura Sterian Ward, Carlos Ueira-Vieira, Luiz Ricardo Goulart

“…RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. …”
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Tomato rot by Rhizopus microsporus alters native fungal community composition and secondary metabolite production by Mmanoko Napo, Alicia Kock, Kazeem A. Alayande, Michael Sulyok, Michael Sulyok, Chibundu N. Ezekiel, Jessie Uehling, Teresa E. Pawlowska, Rasheed A. Adeleke

“…We assessed the impact of the symbiotic R. microsporus harboring its endosymbiont as well as the fungus cured of it on: (1) the magnitude of spoilage in tomato fruits, as evaluated by Koch's postulate for pathogenicity, (2) the shifts in native communities of endophytic fungi inhabiting these fruits, as examined by ITS rRNA gene metabarcoding and (3) secondary metabolites generated by these communities, as analyzed using multi-analyte LC-MS/MS. …”
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Bacterial diversity impacts as a result of combined sewer overflow in a polluted waterway by O. Calderon, H. Porter-Morgan, J. Jacob, W. Elkins

“…At the current study, three sites were sampled one week apart during a dry period and a wet period, where indication of a combined sewage overflow event could be detected. 16s rRNA high-throughput sequencing from these three sites collectively yielded over 1000 species of bacteria belonging to twenty-two classes. …”
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蛋白酪氨酸磷酸酯酶4A2基因的克隆、表达及活性鉴定 by 程超, 郭爱林, 巫国勇, 吴伟康, 罗红鹤, 钟佛添

“…【方法】用RT-PCR技术,从肝癌细胞系HepG2的总RNA中,获得编码PTP4A2基因功能片段的DNA。测序后,通过酶切亚克隆至表达载体pGEX-4T-2,构建重组表达载体,并导入大肠杆菌E.coliBL21中,IPTG诱导表达重组的GST融合蛋白。…”
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شناسایی روابط فیلوژنتیکی گندم های زراعی و اجداد آن بر اساس توالی یابی ژن (HKT) by آرش فاضلی

“…روش های نوین ژنتیکی همچون توالی‌یابی امکان ارزیابی دقیق و سریع مواد ژنتیکی در سطح DNA و RNA را فراهم نموده است. لذا به منظور شناسایی تنوع آللی ژن HKT از گونه‌های زراعی و وحشی گندم استفاده شد. …”
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Effect of miR-1285-3p as a diagnostic biomarker for chronic heart failure on vascular endothelial cells by Chongyang Zhang, Jiazhen He, Dan Xiong, Yi Mei, Yao Zhu, Pan Deng, Yang Duan

“…At the same time, we investigated the effect of miR-1285-3p on vascular endothelial cells. Methods Total RNA was extracted from plasma samples of 106 CHF patients and 106 healthy individuals. …”
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NLRP3 is a BMI-independent mediator of stable COPD by Yonca Gungor, Selin Ercan, Saliha Selin Özuygur Ermiş, Yiğit Kozalı, Gizem Kursunluoglu, Ceyda Sahan, Aylin Ozgen Alpaydin, Hulya Ayar Kayali

“…Peripheral blood mononuclear cells were isolated, and total RNA was extracted for real-time quantitative polymerase chain reaction (qPCR) analysis to measure the expression levels of inflammasome molecules. …”
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