Exploring serum miR-33b as a novel diagnostic marker for hypercholesterolemia and obesity: insights from a pilot case-control study by Yaser Masoumi-Ardakani, Mostafa Eghbalian, Hossein Fallah, Alireza Jafari, Beydolah Shahouzehi

“…MicroRNAs (miRNAs) are small, conserved, non-coding RNA sequences consisting of approximately 22 nucleotides, playing crucial roles in biological and pathological functions. …”
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Sox9-Increased miR-322-5p Facilitates BMP2-Induced Chondrogenic Differentiation by Targeting Smad7 in Mesenchymal Stem Cells by Yongsheng Zeng, Chengcheng Du, Pengcheng Xiao, Yiting Lei, Piao Zhao, Zhenglin Zhu, Shengqiang Gao, Bowen Chen, Shengwen Cheng, Wei Huang, Chen Zhao

“…The purpose of this study was to determine which microRNA is increased by Sox9 and targets Smad7, thus assisting BMP2 in maintaining stable chondrogenesis. …”
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妊娠期高血压患者外周血中的脂代谢基因差异表达

“…【目的】 研究妊娠期高血压疾病患者在孕16~20周外周血白细胞的基因表达谱,寻找妊娠期高血压疾病患者中孕早期外周血白细胞的差异表达基因,并选取异常脂代谢基因进行认证 【方法】 我院2008年8月至2008年12月间在我院产检且经B超确定孕期为16 ~ 20周的初产妇共800例,随访至妊娠结束,将最终发展为子痫前期的6例患者为研究组,40例正常妊娠妇女为对照组,应用人类全基因组cDNA单荧光芯片检测两组孕妇在妊娠16 ~ 20周时外周血白细胞差异基因表达谱;在差异表达基因中选取氧化修饰低密度脂蛋白(oxLDL) 使用酶联免疫吸附试验法(ELISA)检测血浆浓度;血凝素样氧化低密度脂蛋白受体-1 (LOX-1)及载脂蛋白 A5(APOA5)进行RT-PCR测定【结果】 研究组和对照组存在差异表达基因,筛选得到983个差异表达基因,其中719个基因表达上调,264个基因表达下调;研究组oxLDL水平LOX-1水平较正常组明显升高(P < 0.05);研究组APOA5水平较正常组明显降低(P < 0.05);oxLDL水平与LOX-1 mRNA表达水平呈正相关(r为0.91,P < 0.05)【结论】 妊娠期高血压疾病患者外周血白细胞的脂代谢基因oxLDLLOX-1及APOA5基因表达在妊娠16 ~ 20 周时与正常妊娠存在明显差异…”
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Anti-lung Cancer Mechanism of Quercetin Based on Network Pharmacology and Experimental Validation by Yi WANG, Xiaofang PU

“…Cellular experiments indicated that quercetin intervention in low, medium, and high doses significantly inhibited the proliferative activity of A549 cells after 72 hours (P<0.01) and downregulated the mRNA expression levels of PI3K, Akt, and GSK-3β (P<0.05), as well as protein expression trends. …”
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Association of HLA-B27 Gene and Rheumatoid Arthritis: Analysis of Potential Role as a Predictive Biomarker by Karzan Ghafur Khidhir, Baban Ahmad, Dana Khdr Sabir

“…Samples of human blood from RA patients and healthy individuals were collected, and RNA extraction, cDNA synthesis and qPCR were carried out to detect relative expression of HLA-B27. …”
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Measles Outbreak among Previously Immunized Adult Healthcare Workers, China, 2015 by Zhengyi Zhang, Yuan Zhao, Lili Yang, Changhong Lu, Ying Meng, Xiaoli Guan, Hongjin An, Meizhong Zhang, Wenqin Guo, Bo Shang, Jing Yu

“…Fifty-one patients (85%) tested positive for IgM antibodies against the measles virus and 50 patients (83.3%) tested positive for measles virus RNA. Surprisingly, 73.3% of the infected individuals had been previously immunized against measles. …”
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Helicobacter pylori Mutations Conferring Resistance to Fluoroquinolones and Clarithromycin among Dyspeptic Patients Attending a Tertiary Hospital, Tanzania by Hyasinta Jaka, Nele Rüttgerodt, Wolfgang Bohne, Andreas Mueller, Uwe Gross, Christa Kasang, Stephen E. Mshana

“…A total of 208 nonrepetitive biopsies were examined of which 188 (90.4%) tested positive for H. pylori specific 23S rRNA PCR. Clarithromycin resistance mutations were detected in 54/188 (28.7%) of patients tested. …”
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MAC30 Knockdown Inhibits Proliferation and Enhance Apoptosis of Gastric Cancer by Suppressing Wnt/β-Cateninsignaling Pathway by Xiaohong Wu, Yong Zhang, Junping Guo, Xun Yan, Li Shen, Jianping Zhou, Jun Zhao, Min Zhuang, Zhihong Cao

“…Lentiviral vector pGCSIL-shMAC30-GFP of the RNA interference (RNAi) of the MAC30 gene was transfected into gastric cancer BGC-823 cell line and the expression of lentivirus label protein GFP was observed via fluorescence microscope, while cell proliferation and apoptosis were determined with flow cytometry and MTT assay, respectively. …”
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Microbiological effect of topically applied Weissella cibaria on equine pastern dermatitis by Eva Styková, Igor Valocký, Jana Kačírová, Lívia Kolesár Fecskeová

“…A comparison of the initial microbial community based on 16S rRNA amplicon sequencing revealed that there was a statistically significant difference between healthy vs. exudative (R = 0.52, p = 0.003) and exudative vs. proliferative communities (R = 0.78, p = 0.043). …”
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Characterization of the Merkel Cell Carcinoma miRNome by Matthew S. Ning, Annette S. Kim, Nripesh Prasad, Shawn E. Levy, Huiqiu Zhang, Thomas Andl

“…To identify microRNAs specific to MCC (MCC-miRs), next-generation sequencing (NGS) of small RNA libraries was performed on different tissue samples including MCCs, other cutaneous tumors, and normal skin. …”
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Ｅｘｅｎａｔｉｄｅ对多囊卵巢综合征大鼠胰腺组织ＳＩＲＴ１表达的影响

“…【结果】①免疫组化 和Ｑ－ＰＣＲ在蛋白水平和ｍＲＮＡ水平表明ＳＩＲＴ１表达于正常的胰腺内分泌组织中。ＰＣＯＳ组与ＮＣ组相比ＳＩＲＴ１表达明显降低（Ｐ ＜ ０．０５），经过药物干预后的ＥＸ和ＭＦ组与ＰＣＯＳ组相比表达均有不同程度升高（Ｐ ＜ ０．０５），而ＥＸ和ＭＦ之间相比没有统计学差异。…”
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Exosomes as Mediators of Cell-to-Cell Communication in Thyroid Disease by Yi Wang, Feng Xu, Jia-Yu Zhong, Xiao Lin, Su-Kang Shan, Bei Guo, Ming-Hui Zheng, Ling-Qing Yuan

“…Exosomes are a type of extracellular vehicle, formed by budding cell membranes, containing proteins, DNA, and RNA. Concentrated cargoes from parent cells are enveloped in exosomes, which are cell specific and may have functions in the recipient cell, reflecting a novel physiological and pathological mechanism in disease development. …”
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Enterococcus faecium NCIMB 10415 Modulates Epithelial Integrity, Heat Shock Protein, and Proinflammatory Cytokine Response in Intestinal Cells by Shanti Klingspor, Angelika Bondzio, Holger Martens, Jörg R. Aschenbach, Katharina Bratz, Karsten Tedin, Ralf Einspanier, Ulrike Lodemann

“…The ETEC strain decreased transepithelial resistance (TER) and increased IL-8 mRNA and protein expression in both cell lines compared with control cells, an effect that could be prevented by pre- and coincubation with E. faecium. …”
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Impact of psychostimulants on microbiota and short-chain fatty acids alterations in children with attention-deficit/hyperactivity disorder by Nonglak Boonchooduang, Orawan Louthrenoo, Narueporn Likhitweerawong, Chanon Kunasol, Chanisa Thonusin, Sirawit Sriwichaiin, Wichwara Nawara, Nipon Chattipakorn, Siriporn C. Chattipakorn

“…Fecal samples from 30 children aged 6–12 years (10 unmedicated ADHD, 10 medicated ADHD, and 10 healthy controls) were analyzed using 16 S rRNA sequencing and targeted metabolomics. Unmedicated ADHD children show distinct gut microbiota profiles, with lower level of Tyzzerella, Prevotellaceae, and Coriobacteriaceae, compared to controls. …”
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6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy by Qiao You, Jing Wu, Ruining Lyu, Yurong Cai, Na Jiang, Ye Liu, Fang Zhang, Yating He, Deyan Chen, Zhiwei Wu

“…Results 6-TG significantly suppressed EV71 mRNA level, VP1 protein expression, and viral progeny production in HT-29 cells. …”
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The left amygdala is genetically sexually-dimorphic: multi-omics analysis of structural MRI volumes by Yuanyuan Gui, Geyu Zhou, Shuya Cui, Hongyu Li, Hui Lu, Hongyu Zhao

“…We compared transcriptome differences between males and females using data from GTEx and characterized cell-type compositions using GTEx bulk amygdala RNA-seq data and LIBD amygdala single-cell reference profiles. …”
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Exploring the Hepatoprotective Effect of Agaricus bisporus Based on Network Pharmacology by WANG Jie, WANG Jinmei, LIU Hui, LIU Xuguang, GUO Wenjing, HE Hongping

“…The antioxidant capacity in mice was significantly enhanced, as evidenced by a significant increase in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities (P < 0.001) and a significant decrease in malondialdehyde (MDA) content (P < 0.01). Detection of the mRNA and protein expression levels of relevant genes was performed using real-time quantitative polymerase chain reaction (PCR) and Western blot techniques, showing that compared with the model group, the expression of the Bcl-2 in the high-dose group significantly increased (P < 0.001), while the expression of MYC, NF-κB1, RELA and MMP9 significantly decreased (P < 0.01). …”
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Advancing the Fight Against Cervical Cancer: The Promise of Therapeutic HPV Vaccines by Qian Zheng, Misi He, Zejia Mao, Yue Huang, Xiuying Li, Ling Long, Mingfang Guo, Dongling Zou

“…Currently, there are no approved therapeutic HPV vaccines worldwide; however, active progress is being made in clinical research and development using multiple platforms such as peptides, proteins, DNA, RNA, bacterial vectors, viral vectors, and cell-based, each offering relative advantages and limitations for delivering HPV antigens and generating targeted immune responses. …”
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LIM-kinase 1 effects on memory abilities and male courtship song in Drosophila depend on the neuronal type by A. V. Zhuravlev, E. S. Zalomaeva, E. S. Egozova, A. D. Emelin, V. V. Sokurova, E. A. Nikitina, E. V. Savvateeva-Popova

“…We applied GAL4 × UAS binary system to induce limk1 RNA interference in different types of neurons. …”
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Puerarin pretreatment provides protection against myocardial ischemia/reperfusion injury via inhibiting excessive autophagy and apoptosis by modulation of HES1 by Yong Yuan, Songqing Lai, Tie Hu, Fajia Hu, Chenchao Zou, Xiuqi Wang, Ming Fang, Jichun Liu, Huang Huang

“…Nevertheless, the cardioprotective efficacy of Pue pretreatment was negated via the knockdown of HES1 protein expression via pAD/HES1-shRNA transfection. In conclusion, Pue effectively ameliorated HES1-mediated MIRI-induced autophagy, apoptosis, and mitochondrial dysfunction.…”
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