Identification of the etiological agent of equine piroplasmosis in Western and Eastern Siberia by V. A. Rar, V. A. Marchenko, E. A. Efremova, O. V. Suntsova, O. V. Lisak, A. Y. Tikunov, I. V. Meltsov, N. V. Tikunova

“…The analysis of the determined 18S rRNA gene sequences demonstrated that T. equi samples belonged to two genetic groups, which differed significantly by the sequences of the variable (V4) region of the gene. …”
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Hyperlipidemia May Synergize with Hypomethylation in Establishing Trained Immunity and Promoting Inflammation in NASH and NAFLD by Charles I. V. Drummer, Fatma Saaoud, Yu Sun, Diana Atar, Keman Xu, Yifan Lu, Ying Shao, Candice Johnson, Lu Liu, Huimin Shen, Nirag C. Jhala, Xiaohua Jiang, Hong Wang, Xiaofeng Yang

“…We performed a panoramic analysis on both human nonalcoholic steatohepatitis (NASH) microarray data and microarray/RNA-seq data from various mouse models of nonalcoholic fatty liver disease NASH/NAFLD with total 4249 genes examined and made the following findings: (i) human NASH and NAFLD mouse models upregulate both cytokines and chemokines; (ii) pathway analysis indicated that human NASH can be classified into metabolic and immune NASH; methionine- and choline-deficient (MCD)+high-fat diet (HFD), glycine N-methyltransferase deficient (GNMT-KO), methionine adenosyltransferase 1A deficient (MAT1A-KO), and HFCD (high-fat-cholesterol diet) can be classified into inflammatory, SAM accumulation, cholesterol/mevalonate, and LXR/RXR-fatty acid β-oxidation NAFLD, respectively; (iii) canonical and noncanonical inflammasomes play differential roles in the pathogenesis of NASH/NAFLD; (iv) trained immunity (TI) enzymes are significantly upregulated in NASH/NAFLD; HFCD upregulates TI enzymes more than cytokines, chemokines, and inflammasome regulators; (v) the MCD+HFD is a model with the upregulation of proinflammatory cytokines and canonical and noncanonical inflammasomes; however, the HFCD is a model with upregulation of TI enzymes and lipid peroxidation enzymes; and (vi) caspase-11 and caspase-1 act as upstream master regulators, which partially upregulate the expressions of cytokines, chemokines, canonical and noncanonical inflammasome pathway regulators, TI enzymes, and lipid peroxidation enzymes. …”
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Carbon monoxide-oxidising Pseudomonadota on volcanic deposits by Robin A. Dawson, Nicola Fantom, Tamara Martin-Pozas, Patricia Aguila, Gary M. King, Marcela Hernández

“…CV2 (isolated from the 1917 soil). 16S rRNA gene sequencing showed that within the family Burkholderiacea, the genus Paraburkholderia dominated the 2015 volcanic deposit with an average relative abundance of 73.81%, whereas in the 1917 volcanic deposit, Cupriavidus accounted for 33.64% (average relative abundance). …”
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IL-10 and ARG-1 Concentrations in Bone Marrow and Peripheral Blood of Metastatic Neuroblastoma Patients Do Not Associate with Clinical Outcome by Fabio Morandi, Michela Croce, Giuliana Cangemi, Sebastiano Barco, Valentina Rigo, Barbara Carlini, Loredana Amoroso, Vito Pistoia, Silvano Ferrini, Maria Valeria Corrias

“…In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. …”
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Research progress of gut flora in improving human wellness by Chenggang Zhang, Wenjing Gong, Zhihui Li, Dawen Gao, Yan Gao

“…Therefore, “gut flora priority principle” is developed to guarantee human wellness. The 16S rRNA sequencing and mass spectrometric techniques can be used to identify the gut flora, which may guide us to a new era of human wellness based on gut flora wellness. …”
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High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD by Alberto J. León, Emma Gómez, Jose A. Garrote, David Bernardo, Asterio Barrera, Jose L. Marcos, Luis Fernández-Salazar, Benito Velayos, Alfredo Blanco-Quirós, Eduardo Arranz

“…In general, a correlation was observed between mRNA levels of these cytokines and both iNOS and Granzyme B. …”
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Astragalin and rutin restore gut microbiota dysbiosis, alleviate obesity and insulin resistance in high-fat diet-fed C57BL/6J mice by Muni Swamy Ganjayi, Karunakaran Reddy Sankaran, Balaji Meriga, Ruchika Bhatia, Shikha Sharma, Kanthi Kiran Kondepudi

“…In addition, CAR-treated mice showed significantly increased total short-chain fatty acid production, reduced body weight gain, organs’ weights, serum lipid profile (except HDL) and insulin resistance. The mRNA expressions of CCAAT/enhancer binding protein-α (C/EBP-α), sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPAR-γ), acetyl-CoA carboxylase (ACC), adipocyte protein 2 (aP2), and fatty acid synthase (FAS) were downregulated (P < 0.05) and the protein expression of PPAR-γ was downregulated while adenosine 5’ monophosphate-activated protein kinase (AMPK) was phosphorylated in CAR-treated HFD-fed mice compared to the HFD control group. …”
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Betaine enhances SCAPs chondrogenic differentiation and promotes cartilage repair in TMJOA through WDR81 by Meiyue Wang, Zejie Wu, Xiaoyu Zheng, Yishu Huang, Yizhou Jin, Jiaxin Song, Wanzhen Lei, Hua Liu, Riyue Yu, Haoqing Yang, Runtao Gao

“…In vivo cartilage regeneration effects were evaluated by the rat TMJOA model. RNA-sequencing and biological analyses were performed to select target genes and biological processes involved. …”
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Intestinal microbiota is altered in patients with colon cancer and modified by probiotic intervention by Ashley A Hibberd, Anna Lyra, Arthur C Ouwehand, Peter Rolny, Helena Lindegren, Lennart Cedgård, Yvonne Wettergren

“…The colonic and faecal microbiota was assessed by 16S rRNA gene amplicon sequencing.Results The tumour microbiota was characterised by increased microbial diversity and enrichment of several taxa including Fusobacterium, Selenomonas and Peptostreptococcus compared with the control microbiota. …”
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Characterization and Enzymatic Assay of Cellulase-Producing Probiotic Bacteria Isolated from Traditional Fermented Bamboo of Bangladesh by Mauching Marma, Koushik Chakroborty, Jong Min Lee, Zinia Rahman, S. M. Rafiquzzaman

“…Three isolated bacteria, namely FB-1, FB-2, and FB-3, screened based on the carboxymethyl cellulose medium, were tested for biochemical and probiotic properties, and the best isolate was genetically identified by molecular characterization through 16S rRNA gene sequencing and evolutionary analysis. All three gram-positive isolates were observed to be positive for starch hydrolysis and fermentation of sucrose, suggesting their ability to breakdown carbohydrates while testing negative for gelatinase, indole, and H2S test. …”
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Finding potential inhibitors from phytochemicals against nucleoprotein of crimean congo fever virus using in silico approach by Muhammad Ramzan, Sajid Mahmood, Adnan Amjad, Mohsin Javed, Ammar Zidan, Ali Bahadur, Shahid Iqbal, Muhammad Saad, Namrah Zaka, Shazia Khurshid, Nasser S. Awwad, Hala A. Ibrahium, Toheed Akhter

“…Abstract The Crimean Congo virus has been reported to be a part of the spherical RNA-enveloped viruses from the Bunyaviridae family. …”
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不同级别胚胎对小鼠子宫内膜的诱导作用研究

“…LIF的表达情况;移植后第7天取出子宫,观察胚胎种植情况?【结果】 各组间HBEGF 和LIF的mRNA及蛋白的表达水平均具有显著统计学差异(P均 < 0.001)?组间比较发现:8c+4c与8c+2c两组间HBEGF?…”
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Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction by Sayed Sajid Hussain, Fan Zhang, Yuanyuan Zhang, Kiran Thakur, Mahrukh Naudhani, Carlos L. Cespedes-Acuña, Zhaojun Wei

“…For cell cycle arrest, the mRNA expression levels of p21, p27 and p53 were up-regulated, while the expression levels of Cyclin A, Cyclin D1, Cyclin E and CDK1/2 were down-regulated. …”
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Polyphenols synergistic drugs to ameliorate non-alcoholic fatty liver disease via signal pathway and gut microbiota: A review by Hongcai Li, Jingjing Liang, Mengzhen Han, Zhenpeng Gao

“…Furthermore, some emerging mechanisms such as microRNA potential biomarker therapies may provide therapeutic avenues for NAFLD. …”
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Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications by Yurong Chen, Haibo Zhu, Fucheng Guo, Luyao Wang, Wenli Zhang, Ruizhi Liu, Xiaoling Zhang, Xiangpeng Dai

“…The related gene expression and transcriptome profiles were investigated by RT-qPCR and RNA-seq, respectively. Blastocyst and implantation frequencies were not significantly affected by vitrification. …”
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DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells by Siti Razila Abdul Razak, Yukihiro Baba, Hiromitsu Nakauchi, Makoto Otsu, Sumiko Watanabe

“…We have been analyzing comprehensive expression patterns of microRNA in human and mouse embryonic stem and induced pluripotent stem cells. …”
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Spermidine alleviates thymopoiesis defects and aging of the peripheral T-cell population in mice after radiation exposure by Kengo Yoshida, Zhenqiu Liu, Yoshiko Kubo, Masahiko Miura, Mika Yamaoka, Hiroko Nagamura, Munechika Misumi, Yoichiro Kusunoki

“…The numbers of total, double-positive, and single-positive thymocytes were decreased by irradiation, whereas none were decreased in the irradiated mice treated with SPD. RNA sequencing of thymus cells revealed 803 upregulated genes in irradiated mice compared with those in non-irradiated control mice, with these genes enriched in leukocyte activation and inflammatory cytokine production. …”
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Ceftriaxone-associated dysbiosis decreases voriconazole bioavailability by upregulating intestinal P-glycoprotein expression through activation of the Nrf2-mediated signalling path... by Xiaokang Wang, Xiaokang Wang, Chunxiao Ye, Xixiao Yang, Maoxun Yang, Maoxun Yang, Maoxun Yang

“…The composition of the intestinal flora was examined using 16S rRNA sequencing analysis. Voriconazole concentrations were determined by high-performance liquid chromatography-tandem mass spectrometry. …”
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Impact of Age and Sex on Viral Load in Hepatitis C Virus Infection by Andrea Magri, Giulia Francesca Manfredi, Carlo Smirne, Silvia Pigni, Michela Emma Burlone, Mattia Bellan, Nicole Vercellino, Rosalba Minisini, Mario Pirisi

“…Females were older (median age 68 vs. 57, <i>p</i> < 0.001) and had a higher prevalence of genotype 2 (33% vs. 20%, <i>p</i> < 0.001) than males; there was no difference between sexes regarding the METAVIR stage. The median HCV RNA concentration was 1.017 × 10⁶ IU/mL (interquartile range, 0.286–2.400). …”
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Kruppel-family zinc finger proteins as emerging epigenetic biomarkers in head and neck squamous cell carcinoma by Patrick Pearson, Kendra Smith, Nilita Sood, Elizabeth Chia, Alicia Follett, Michael B. Prystowsky, Simon Kirby, Thomas J. Belbin

“…Results In 20 patients from the TCGA cohort of HNSCC patients where ZNF154 and ZNF132 DNA methylation and RNA expression could be compared in tumor and adjacent normal tissue, there was increased DNA methylation and decreased expression of both ZNF154 and ZNF132 in primary tumours. …”
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