Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. by Sabiha S. Salih, Shno J. Mohammed, Imad M Noori, Lana MA Mohammed, Taib A. Hama Soor

“…Molecular identification of Proteus bacteria was confirmed by PCR amplification of part of 16S rRNA using Proteus specific set of primers.  70 meat samples (cattle, sheep, chicken, turkey, goat, and fish) were collected in local meat shops in the center of Sulaimani city. 29 (41.4%) samples were positive to Proteus species and 22 (75.87%) isolates were P. mirabilis and seven (24.13%) were P. vulgaris based on conventional biochemical tests. …”
Get full text

Rumen Bacterial Community Responses to Three DHA Supplements: A Comparative In Vitro Study by Jianmin Zou, Genna Ba, Dian Wang, Mengmeng Li, Shaohong Jin, Chong Chen, Wei Tan, Jian He, Hengsheng Du, Pengjie Wang, Yinhua Zhu

“…The concentration of supplements has a significant impact on the DHA loss rate and algal oil exhibited the highest rate of loss, but bioaccessibility was not significantly different from the other supplements. 16S rRNA sequencing showed that three DHA supplements altered the bacterial composition of in vitro batch cultures inoculated with rumen microorganisms from cows, and caused changes in the relative abundance of important bacterial phyla, families, and genera. …”
Get full text

Development of an m6A-Related lncRNAs Signature Predicts Tumor Stemness and Prognosis for Low-Grade Glioma Patients by Dahua Xu, Peihu Li, Chunrui Zhang, Yutong Shen, Jiale Cai, Qingchen Wei, Meng Cao, Zhizhou Xu, Deng Wu, Hong Wang, Xiaoman Bi, Bo Wang, Kongning Li

“…A multicenter transcriptome analysis of lncRNA expression in 1,247 LGG samples was performed in this study. …”
Get full text

Antimicrobial and antiproliferative activity of biosynthesized manganese nanocomposite with amide derivative originated by endophytic Aspergillus terreus by Nashwa El-Gazzar, Reem Farouk, Nervana S. Diab, Gamal Rabie, Basel Sitohy

“…The most potent isolate was identified utilizing 18S rRNA and was applied to purify and separate their natural antimicrobial products by Flash column chromatography. …”
Get full text

Opposing Roles of Leptin and Ghrelin in the Equine Corpus Luteum Regulation: An In Vitro Study by António Galvão, Angela Tramontano, Maria Rosa Rebordão, Ana Amaral, Pedro Pinto Bravo, Anna Szóstek, Dariusz Skarzynski, Antonio Mollo, Graça Ferreira-Dias

“…Thus, the aim of this study was: (i) to characterize the immunolocalization, mRNA and protein levels of leptin (LEP), Ghrelin (GHR) and respective receptors LEPR and Ghr-R1A, throughout luteal phase; and (ii) to evaluate the role of LEP and GHR on progesterone (P4), prostaglandin (PG) E2 and PGF2α, nitric oxide (nitrite), tumor necrosis factor-α (TNF); macrophage migration inhibitory factor (MIF) secretion, and on angiogenic activity (BAEC proliferation), in equine corpus luteum (CL) from early and mid-luteal stages. …”
Get full text

The role of SASP in ischemic stroke: a deep dive into cellular mechanisms by Dong Xie, Yang Liu, Fang-Biao Xu, Jin-Sheng Zhang

“…RT-qPCR demonstrated a marked elevation in Lcp1 mRNA and a pronounced reduction in Ccl3 in the MCAO group relative to the Sham group.ConclusionThe Ccl3 gene in basophils and its immune cell interaction is a linchpin in the IS immune microenvironment. …”
Get full text

Different functions of PHF10 isoforms – subunits of the PBAF chromatin remodeling complex by A. A. Sheynov, V. V. Tatarskiy, A. M. Azieva, S. G. Georgieva, N. V. Soshnikova

“…These isoforms contain PHD (plant homeodomain) fingers for nucleosome binding and recruit RNA polymerase II on the promoters of cell cycle genes. …”
Get full text

Isolation and optimization of Bacillus thuringiensis BRB-3 isolated from germinated black upland rice seeds for GABA production by Rungnapha Wannasutta, Sophon Boonlue, Nuntavun Riddech, Wiyada Mongkolthanaruk

“…Further identification of the BRB-3 strain was performed using 16S rRNA gene sequence, which placed it in Bacillus cereus group. …”
Get full text

Multiomic quantification of the KRAS mutation dosage improves the preoperative prediction of survival and recurrence in patients with pancreatic ductal adenocarcinoma by Won-Gun Yun, Daeun Kim, Youngmin Han, Wooil Kwon, Seong-Geun Lee, Jin-Young Jang, Daechan Park

“…In this retrospective cohort study, we analyzed 193 surgically treated patients with PDAC between 2009 and 2016. RNA, whole-exome, and KRAS-targeted sequencing data were used to estimate the dosage of the KRAS G12 mutant. …”
Get full text

miR-7-5p Promotes Hepatic Stellate Cell Activation by Targeting Fibroblast Growth Factor Receptor 4 by Shuxia Tian, Min Chen, Bing Wang, Yonglong Han, Haonan Shang, Junming Chen

“…Fibroblast growth factor receptor 4 (FGFR4) is a key mediator that protects the liver from chronic injury. MicroRNA-7 (miR-7) is a tumor suppressor and associated with lipid homeostasis in the liver. …”
Get full text

可溶性TAT PTD-Ngb融合蛋白的原核表达、鉴定与纯化及其对皮质神经元的跨膜转导 by 周国钰, 胡学强, 胡骏, 陆正齐, 楼之茵, 朱灿胜, 熊洁萍

“…【目的】构建含蛋白转导域（protein transduction domain,PTD）与脑红蛋白（neuroglobin,Ngb）融合基因的表达质粒,原核表达可溶性TATPTD-Ngb,并鉴定、纯化,检测TATPTD-Ngb对皮质神经元的跨膜转导功能。【方法】提取SD大鼠脑组织总RNA,通过RT-PCR法构建编码TATPTD-Ngb的融合基因,克隆入pET28b原核表达载体,转化入大肠杆菌BL21（DE3）pLysS,诱导表达,表达产物进行SDS-PAGE、Western-blot分析及可溶性鉴定,利用所表达的目的蛋白上的6个组蛋白用Ni-NTA琼脂进行亲和层析纯化,将不同浓度纯化的融合蛋白与原代培养皮质神经元共孵育2h,用Western-blot分析融合蛋白的跨膜转导。…”
Get full text

Mechanisms of multidrug resistance caused by an Ipi1 mutation in the fungal pathogen Candida glabrata by Taiga Miyazaki, Shintaro Shimamura, Yohsuke Nagayoshi, Hironobu Nakayama, Akihiro Morita, Yutaka Tanaka, Yasuhiko Matsumoto, Tatsuo Inamine, Hiroshi Nishikawa, Nana Nakada, Makoto Sumiyoshi, Tatsuro Hirayama, Shigeru Kohno, Hiroshi Mukae

“…The drug-resistant phenotype is due to a non-synonymous mutation (R70H) in gene IPI1, which is involved in pre-rRNA processing. Azole resistance in the ipi1 R70H mutant depends on the Pdr1 transcription factor, which regulates the expression of multidrug transporters. …”
Get full text

Tunable control of Cas12 activity promotes universal and fast one-pot nucleic acid detection by Zhou-Hua Cheng, Xi-Yan Luo, Sheng-Song Yu, Di Min, Shu-Xia Zhang, Xiao-Fan Li, Jie-Jie Chen, Dong-Feng Liu, Han-Qing Yu

“…This method uses heparin sodium to precisely tunes the cis-cleavage capability of Cas12 via interference with the Cas12a-crRNA binding process, thereby generating significant fluorescence due to the accumulation of isothermal amplification products. …”
Get full text

Differentially expressed transcript isoforms associate with resistance to tuberculin skin test and interferon gamma release assay conversion. by Jason D Simmons, R Max Segnitz, Kimberly A Dill-McFarland, Catherine M Stein, Glenna J Peterson, Harriet Mayanja-Kizza, W Henry Boom, Thomas R Hawn

“…<h4>Materials and methods</h4>Monocytes from 49 RSTR versus 52 subjects with latent Mtb infection (LTBI) were infected with M. tuberculosis (H37Rv) or left unstimulated (media) prior to RNA isolation and sequencing. RSTR-associated gene expression was then identified using differential transcript isoform analysis.…”
Get full text

Panzerina lanata accelerates methicillin-resistant Staphylococcus aureus eradication by promoting migration and activation of neutrophils by Shuai Dong, Xingyuan Bai, Bin Chen, Minzhe Fan, Qi Liu, Yubo Zhao, Linsen Li, Dan Zhu

“…Using an air pouch MRSA infection mouse model, the number of neutrophils, reactive oxygen species (ROS) level in neutrophils, and neutrophil extracellular trap (NET) formation were dynamically detected by flow cytometry and immunofluorescence. RNA-seq, RT-qPCR, flow cytometry, ELISA, and CXC chemokine receptor 2 (CXCR2) and P-selectin glycoprotein ligand-1 (PSGL-1) inhibitors were used to explore the mechanism of Lanata in regulating neutrophils.ResultsIn vitro assays showed that Lanata had no direct antibacterial activity. …”
Get full text

Testing the Purity of <i>Limnospira fusiformis</i> Cultures After Axenicity Treatments by Michael Schagerl, Alexander Kaptejna, Fabian Polz, Sameh S. Ali, Shuhao Huo, Joana Seneca, Petra Pjevac, Vera Hechtl

“…Contamination was also verified by cultivation-independent methods like flow cytometry and 16S rRNA genome amplicon sequencing. We detected taxa of the phyla Proteobacteria, Bacteriodota, Firmicutes and to a lesser extent Verrucomicrobiota. …”
Get full text

Odontogenic differentiation of dental pulp stem cells by glycogen synthase kinase-3β inhibitory peptides by Vidhyashree Rajasekar, Mohamed Mahmoud Abdalla, Mohammed S. Basbrain, Prasanna Neelakantan, Cynthia KY Yiu

“…The differentiation of hDPSCs following peptide treatment was determined using the alkaline phosphatase assay (ALP), calcium mineralization (alizarin red staining), and quantification of mRNA expression of differentiation markers via quantitative real-time polymerase chain reaction. …”
Get full text

矢车菊素－３－葡萄糖苷通过降低ＳＴＡＴ３活化抑制ＴＮＦ－α诱导的小鼠血管平滑肌细胞增殖

“…【方法】 Ｃ５７ＢＬ／６Ｊ小鼠主动脉平滑肌细胞（ＶＳＭＣ） 购于ＡＴＣＣ，体外培养后以Ｃ３Ｇ对细胞进行预处理后观察ＴＮＦ－α的促细胞增殖作用；Ｄｉｈｙｄｒｏｅｔｈｉｄｉｕｍ （ＤＨＥ）荧光染色检测ＶＳＭＣ在ＴＮＦ－α作用下的活性氧（ＲＯＳ）生成情况；实时定量ＰＣＲ检测细胞内ＮＡＤＰＨ氧化酶活化蛋白１（ＮｏｘＡ１）的ｍＲＮＡ水平；蛋白质印迹法检测细胞内ＮｏｘＡ１、信号转导与转录激活因子３ （ＳＴＡＴ３）、磷酸化ＳＴＡＴ３及β－ａｃｔｉｎ的表达水平。…”
Get full text

The Role of Autophagy and Related MicroRNAs in Inflammatory Bowel Disease by Shiyuan Wang, Yan Huang, Cili Zhou, Huangan Wu, Jimeng Zhao, Luyi Wu, Min Zhao, Fang Zhang, Huirong Liu

“…Accumulating evidence demonstrates that microRNA- (miR-) mediated posttranscriptional regulation plays an important role in autophagy in inflammatory bowel disease (IBD), a disease that is difficult to manage clinically because of the associated chronic recurrent nonspecific inflammation. …”
Get full text

SIRT1/PGC-1α-mediated mitophagy participates the improvement roles of BMAL1 in podocytes injury in diabetic nephropathy: evidences from in vitro experiments by Yanxia Rui, Yinfeng Guo, Linying He, Min-er Wang, Henglan Wu

“…Results In HG-induced podocyte injury model, we found that BMAL1 and SIRT1 mRNA level was significantly decreased, and positively correlated with mitophagy dysfunction. …”
Get full text