Pirfenidone Inhibits Hypoxic Pulmonary Hypertension through the NADPH/ROS/p38 Pathway in Adventitial Fibroblasts in the Pulmonary Artery by Song Zhang, ZongXiu Yin, WeiDong Qin, XiaoLi Ma, Yao Zhang, EnXiu Liu, YanBiao Chu

“…NOX4 and Rac1 were inhibited or overexpressed by shRNA or pcDNA3.1, respectively. Proliferation of HPAAFs was quantified by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays to assess cellular metabolic activity, cell counts, and ethynyldeoxyuridine (EdU) assays to detect DNA synthesis. …”
Get full text

Molecular Characterization and Phylogenetic Analysis of Listeria monocytogenes Isolated from Milk and Milk Products in Kaduna, Nigeria by U. B. Usman, J. K. P. Kwaga, J. Kabir, O. S. Olonitola, S. Radu, F. Bande

“…Sequence and phylogenetic analysis based on the 16S rRNA revealed that Nigerian L. monocytogenes isolates (NGA 34A, NGA 35A, NGA 41A, and NGA 38A), when compared with reference L. monocytogenes, were grouped into two distinct clusters, A and B, with sequence (NGA 34A, NGA 35A, and NGA 41A) phylogenetically closer to J1776; N1-011A; R2-502; J1816; and J2-031, whereas L. monocytogenes isolate (NGA 38A) clustered with EDG; J1-220; J1926; J1817; and J2-1091. …”
Get full text

Distinctive Gut Microbiota Alteration Is Associated with Poststroke Functional Recovery: Results from a Prospective Cohort Study by Yini Dang, Xintong Zhang, Yu Zheng, Binbin Yu, Dijia Pan, Xiaomin Jiang, Chengjie Yan, Qiuyu Yu, Xiao Lu

“…Their fecal DNAs were extracted, and the V3-V4 regions of the conserved bacterial 16S RNA were amplified and sequenced on the Illumina MiSeq platform. …”
Get full text

Anthracophyllum sontraense (Omphalotaceae, Agaricales), A New Species from Danang, Vietnam by Trung Thanh Nguyen, Hanh Hong Do, Toan Nguyen-Sy, Uyen Thi Diem Huynh, Nguyen Thi Lam Hai, Thi Thanh Tam Phan, Thi Thu Hoai Nguyen, Do Tan Khang, Phu Thi Tran

“…The phylogenetic analyses based on the combined internal transcribed spacer (ITS) and Large Subunit (LSU) molecular markers of ribosomal RNA genes revealed that the two specimens clustered in a single clade at species level with all tested Anthracophyllum species with strong bootstrap support of 99%. …”
Get full text

Gene Expression Differences Predict Treatment Outcome of Merkel Cell Carcinoma Patients by Loren Masterson, Bryan J. Thibodeau, Laura E. Fortier, Timothy J. Geddes, Barbara L. Pruetz, Rajwant Malhotra, Richard Keidan, George D. Wilson

“…Laser capture microdissection was used to isolate tumor cells for subsequent RNA isolation and gene expression analysis with Affymetrix GeneChip Human Exon 1.0 ST arrays. …”
Get full text

Advancements in Managing Anthracycline-Induced Cardiotoxicity: Insights from Interventional Clinical Trials by Zhao M, Zhang X, Zhou D, Song J

“…Advanced monitoring techniques, including global longitudinal strain (GLS)-guided echocardiography, cardiac magnetic resonance (CMR) and novel biomarkers (cfDNA, microRNA), showed promise in early AIC detection. Exercise interventions demonstrated significant cardiovascular benefits.Conclusion: Cardioprotective agents, early detection methods, and exercise interventions are key to managing AIC. …”
Get full text

SMYD3 drives cell cycle and epithelial-mesenchymal transition pathways through dual gene transcriptional repression and activation in HPV-negative head and neck cancer by Madhavi Murali, Abbas Saeed, Sohyoung Kim, Kyunghee Burkitt, Hui Cheng, Arfa Moshiri, Jawad Akhtar, Daniel Tsai, Marie Luff, Baktiar Karim, Vassiliki Saloura

“…In this study, we show that SMYD3 depletion using siRNA interference or CRISPR decreases cellular proliferation and clonal capacity, induces cell cycle arrest and decreases the invasive potential of HPV-negative HNSCC cell lines. …”
Get full text

Gut microbial alteration in chronic spontaneous urticaria unresponsive to second generation antihistamines and its correlation with disease characteristics‐ a cross‐sectional case‐... by Indrashis Podder, David Pesqué, Nerea Carrón, Pedro Iñaki González Torres, Ramon M. Pujol, Ana M. Giménez‐Arnau

“…Clinico‐demographic profile, laboratory investigations and stool analysis were conducted in all study participants. 16S RNA gene sequencing and DNA isolation was performed for all stool samples, followed by bioinformatic analysis. …”
Get full text

Five New Indole Alkaloid Derivatives from Deep-Sea Fungus <i>Aspergillus fumigatus</i> AF1 by Lai-Hui Dai, Gao-Rong Zhang, Yang-Hui Ou, Xiao-Jing Liu, Hong-Liang Yao, Wen-Hao Hu, Hou-Jin Li, Wen-Jian Lan

“…Compounds <b>8</b> and <b>13</b> displayed significant downregulation of the mRNA expression levels of all three molecular markers (COL1A1, α-SMA and FN1), with compound <b>13</b> exhibiting the best performance among all the tested compounds.…”
Get full text

Effects of Chenpi (Citrus reticulata cv. Chachiensis) on serum antioxidant enzymes, inflammatory factors, and intestinal health in Beagle dogs by Wencan Wang, Ling Xu, Yan Zhang, Yong Cao, Yixue Yang, Guo Liu, Guo Liu, Xin Mao

“…Analysis of the microbial composition via 16S rRNA sequencing showed that CPP increased Bacteroidota and decreased Firmicutes in the gut flora at the phylum level. …”
Get full text

Effect of extracellular vesicle ZNF280B derived from lung cancer stem cells on lung cancer progression by Qixia Guo, Jiayan Lu, Hui Zhao, Ding Zhou, Hua Liu

“…RT-qPCR was utilized to assess the mRNA expression levels of these genes, while Western blotting was used to evaluate the protein expression levels of ZNF280B and P53. …”
Get full text

Keratinocyte differentiation factor 1 enhances cervical cancer cell viability and migration by activating the PI3K/AKT pathway by Chao Chen, Junhua Liao, Xingxing Sun

“…Reverse transcription polymerase chain reaction was used to detect the mRNA expression of KDF1 in cervical cancer tissues and cells. …”
Get full text

Discovery of novel PRMT1 inhibitors: a combined approach using AI classification model and traditional virtual screening by Jungan Zhang, Yixin Ren, Yixin Ren, Yun Teng, Han Wu, Jingsu Xue, Lulu Chen, Xiaoyue Song, Yan Li, Ying Zhou, Zongran Pang, Zongran Pang, Hao Wang, Hao Wang, Hao Wang

“…Protein arginine methyltransferases (PRMTs) play crucial roles in gene regulation, signal transduction, mRNA splicing, DNA repair, cell differentiation, and embryonic development. …”
Get full text

Age and diet affect self-resilience of intestinal microbiome in mice by Chenyi Shao, Shenmin Chen, Huan Yang, Mufan Li, Yinhui Liu, Shu Wen, Jing Xiao, Li Tang

“…Microscopy was used to observe morphological changes in the mucosa. In addition, 16S rRNA sequencing and polymerase chain reaction-denaturing gradient gel electrophoresis were performed to identify the bacterial taxa and microbiome structure, respectively.ResultsThe diversity of the gut microbiota in infant mice was recovered by the sixth week, while relative abundance of Ruminococcaceae_UCG_014 was low and did not return to normal levels. …”
Get full text

Genes underlying cold acclimation in the tea plant (<i>Camellia sinensis</i> (L.) Kuntze) by L. S. Samarina, L. S. Malyukova, M. V. Gvasaliya, A. M. Efremov, V. I. Malyarovskaya, S. V. Loshkareva, M. T. Tuov

“…The most recent studies show an important role of miRNA in the mechanisms of response to chilling and freezing in tea. …”
Get full text

Blood from septic patients with necrotising soft tissue infection treated with hyperbaric oxygen reveal different gene expression patterns compared to standard treatment by Julie Vinkel, Alfonso Buil, Ole Hyldegaard

“…Methods An observational cohort study examining 83 NSTI patients treated with HBO2 in the acute phase of NSTI, fourteen of whom had received two sessions of HBO2 (HBOx2 group), and another ten patients (non-HBO group) who had not been exposed to HBO2. Whole blood RNA sequencing and clinical data were collected at baseline and after the intervention, and at equivalent time points in the non-HBO group. …”
Get full text

Transcriptomic signatures of severe acute mountain sickness during rapid ascent to 4,300 m by Ruoting Yang, Aarti Gautam, Rasha Hammamieh, Robert C. Roach, Beth A. Beidleman

“…Blood samples were collected from participants at SL and HA. RNA sequencing was employed to profile gene expression. …”
Get full text

Cyanidin-3-O-Glucoside Modulates the In Vitro Inflammatory Crosstalk between Intestinal Epithelial and Endothelial Cells by Daniela Ferrari, Francesco Cimino, Deborah Fratantonio, Maria Sofia Molonia, Romina Bashllari, Rossana Busà, Antonella Saija, Antonio Speciale

“…Furthermore, TNF-α-stimulated Caco-2 cells induced endothelial cells activation with increased E-selectin and VCAM-1 mRNA, leukocyte adhesion, and NF-κB levels in HUVECs, which were inhibited by C3G. …”
Get full text

Thrombin Induces COX-2 and PGE2 Expression via PAR1/PKCalpha/MAPK-Dependent NF-kappaB Activation in Human Tracheal Smooth Muscle Cells by Chien-Chung Yang, Li-Der Hsiao, Ya-Fang Shih, Chih-Kai Hsu, Chia-Yu Hu, Chuen-Mao Yang

“…Our results verified that thrombin markedly triggered PGE2 secretion via COX-2 upregulation which were diminished by the inhibitor of thrombin (PPACK), PAR1 (SCH79797), Gi/o protein (GPA2), Gq protein (GPA2A), PKCα (Gö6976), p38 MAPK (SB202190), JNK1/2 (SP600125), MEK1/2 (U0126), or NF-κB (helenalin) and transfection with siRNA of PAR1, Gqα, Giα, PKCα, JNK2, p38, p42, or p65. …”
Get full text

人工养殖林麝脓肿病病原菌的分离与鉴定 by 吴世洲 1 #杨林富 1 #钱丽敏 1 杨明华 2 李琦华 2 陈培富 1

“…结果表明：皮下和肝脏脓肿血琼脂培养均获得乳白色菌落，16S rRNA基因序列分析显示，其病原菌分别为黏质沙雷菌（Serratia marcescens，3株）和大肠杆菌（Escherichia coli，1株）。…”
Get full text