Evaluation of Inflammation-Related Proteins in Multiple Sclerosis Disease with Relapses and Remissions by Nisa Hocaoğlu, Nur Damla Korkmaz, Birsen Elibol, Zehra Cemre Karakayalı, Şule Terzioğlu-Uşak, Azize Esra Gürsoy

“…Lymphocytes were isolated from peripheral blood. After RNA isolation and cDNA conversion, NLRP3, ASC, NLRX1, IL-1β, and IL-18 gene expressions were measured in real time-polymerase chain reaction. …”
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The role of Ca2+ signalling and InsP3R in the pathogenesis of intrahepatic cholestasis of pregnancy by Dan Pan, Mengting Jiang, Guoxian Tao, Jinmei Shi, Zhiwei Song, Ren Chen, Dongguo Wang

“…The expression of InsP3R protein in the liver, and serum levels of Ca2+, glycocholic acid and bile acid were detected.Results InsP3R mRNA and protein were significantly lower in the ICP model group compared to the normal group, as determined by qPCR and immunohistochemistry, respectively. …”
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Annexin A5 knockdown inhibits cardiomyocyte apoptosis and alleviates cardiac hypertrophy via activating the PI3K/AKT/Bcl-2 signaling pathway by Lina Zhao, Hongjuan Cao, Yao Yuan, Chunyan Liao, Dan Huang, Xiaoyi Li, Yueyao Zhao, Quanfeng Huang, Sha Li, Bei Zhang

“…The expression of ANXA5 protein and mRNA was overexpressed, and knockdown of ANXA5 can effectively attenuate cardiomyocyte apoptosis and inflammatory response, ameliorate myocardial hypertrophy and cardiac dysfunction in ALD-induced hypertrophic cardiomyocytes and in SHR hypertrophic hearts. …”
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Antimicrobial peptide AP2 ameliorates Salmonella Typhimurium infection by modulating gut microbiota by Lianglan Li, Qiufen Mo, Yi Wan, Yuanhao Zhou, Weiqin Li, Weifen Li

“…These effects were evidenced by reduced ST-induced body weight loss and lower levels of serum inflammatory cytokines. A 16 S rRNA-based analysis of the cecal microbiota revealed that AP2 significantly modulated the gut microbiota, increasing the relative abundance of Bifidobacterium while decreasing that of Akkermansia at the genus level. …”
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大鼠BDNF基因复制缺陷型重组腺病毒的构建与鉴定 by 王鹏, 吴燕峰, 黄文林, 唐勇, 黄霖, 杨睿, 沈慧勇

“…【目的】构建携带大鼠脑源性神经营养因子（brain derived neurotrophic factor，BDNF）目的基因的复制缺陷型重组腺病毒，并初步检测其体外表达目的基因的能力。【方法】提取大鼠脑组织总RNA，使用RT—PCR方法扩增BDNF目的基因并将其克隆入pMD18-T Simple载体测序，将测序完全正确的BDNF目的基因克隆入pShuttle2中，然后利用体外连接法将BDNF目的基因克隆入Adeno—X^TM腺病毒骨架中。…”
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In vitro and In silico investigation deciphering novel antifungal activity of endophyte Bacillus velezensis CBMB205 against Fusarium oxysporum by Vibha R, Daniela Loaiza Granada, Sinosh Skariyachan, Ujwal P, Sandesh k

“…In the present study, the endophytic bacterium was isolated from Globba racemosa and their antagonistic activities against Foc were studied, and the probable molecular mechanism of antagonism was predicted by molecular docking studies. The 16SrRNA sequencing confirmed the endophytic isolate to be Bacillus velezensis CBMB205 (EG2). …”
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ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway by Xi Zhu, Xiaogang Zhang, Xinpeng Cong, Luoning Zhu, Zhongping Ning

“…The fibrosis was evaluated with Masson’s trichrome staining in the atrial myocardium. mRNA levels of IL-1β, IL-6, collagen I, and collagen III were measured using real-time qRT-PCR. …”
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Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment by Ashley E Kramer, Kathryn M Ellwood, Nicole Guarino, Cong-Jun Li, Aditya Dutta

“…This study investigates the genetic drivers mediating the transition of pre-recruitment follicles to the pre-ovulatory phase, a crucial stage before ovulation. Using RNA sequencing and bioinformatics approaches such as a differential gene expression analysis, we compared pre-recruitment follicles with the recently recruited F5 pre-ovulatory follicle to identify key genes and upstream regulators involved in this transition. …”
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Effect of Electroacupuncture at Shenting and Baihui Acupoints on Ability of Learning and Memory and Expression of Autophagy Related Genes and Proteins in Cerebral Ischemia-reperfus... by Lingli GAO, Xiaodong FENG

“…Morris water maze experiment was used to observe learning and memory ability of rats, TTC staining was used to observe the change of brain infarction area of rats, RT-PCR and Western Blot were used to detect the expression of autophagy related genes and proteins in hippocampus.Results:④Morris water maze outcomes showed that compared with the model group, the escape latency time of rats in the EA group was obviously shortened, the number of across-platform was increased and the distance was shortened (<italic>P</italic>&lt;0.05).④Compared with the model group, the nerve defect scale of brain ischemia-reperfusion injury rats in the EA group was decreased (<italic>P</italic>&lt;0.05).④TTC staining results showed that the area of brain infarction in the EA group and model group was respectively higher than that in the sham operation group, and the volume of brain infarction in the EA group was reduced compared with the model group (<italic>P</italic>&lt;0.05).④Compared with the model group, the levels of Beclin 1, LC3Ⅰ/Ⅱand PI3K mRNA and protein in the left side of hippocampus in the EA group were increased (<italic>P</italic>&lt;0.05).Conclusion:EA at the DU24 and DU20 acupoints can reduce the cerebral infarction, and improve animal behaviors, and protect the neuronal injury. …”
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改变组织醛固酮及其受体表达对SHR大鼠肾脏纤维化的影响 by 成彩联, 娄探奇, 汤颖, 石成钢, 陈珠江

“…【方法】8周龄的雄性自发性高血压大鼠（SHR）24只及同源正常京都大鼠（WKY）8只，SHR分为贝那普利干预组[30ing／（kg·d）]、大剂量安体舒通干预组[100mg／（kg·d）]和高血压模型对照组，同时设同源的正常对照组，干预时间为8周，检测收缩压、尿蛋白、血白蛋白、尿素氮、肌酐、肾组织醛固酮受体、TGF-β1的mRNA和蛋白表达。【结果】贝那普利能下调组织醛固酮【（15．8±2．6）vs（22．2±0．6）pg／mg]及其受体水平【（15±4）vs（13±5）PU]并减轻肾脏纤维化[（17．0±1．8）vs（20．0±2．3）PU]，均为P〈0．05；大剂量安体舒通能上调组织醛固酮[（24．3±4．6）vs（22．2±0．6）pg／mg]及其受体水平[（16±6）vs（13±5）PU]并加重肾脏纤维化[（22．6±3．0）vs（20．0±2．3）PU]，均为P〈0．05。…”
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MRP8/ABCC11 Expression Is Regulated by Dexamethasone in Breast Cancer Cells and Is Associated to Progesterone Receptor Status in Breast Tumors by Mylène Honorat, Aurélia Mesnier, Julie Vendrell, Attilio Di Pietro, Valérie Lin, Charles Dumontet, Pascale Cohen, Léa Payen

“…The ATP-binding cassette multidrug resistance protein 8 (MRP8/ABCC11) mediates the excretion of anticancer drugs. ABCC11 mRNA and protein levels were enhanced by DEX (dexamethasone) and by PROG (progesterone) in MCF7 (progesterone receptor-(PR-) positive) but not in MDA-MB-231 (PR-negative) breast cancer cells. …”
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Cryo-thermal therapy reshaped the tumor immune microenvironment to enhance the efficacy of adoptive T cell therapy by Shicheng Wang, Peng Peng, Junjun Wang, Zelu Zhang, Ping Liu, Lisa X. Xu

“…The right large tumor was subjected to CTT, and the left small tumor was collected for flow cytometry, RNA-seq, immunohistochemistry and TCR Vβ sequencing. …”
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Mapping dynamic regulation of gene expression using single-cell transcriptomics and application to complex disease genetics by Hanna Abe, Phillip Lin, Dan Zhou, Douglas M. Ruderfer, Eric R. Gamazon

“…However, the identification of gene-level associations in distinct cell types faces several challenges, including the limited reference resources from population-scale studies, data sparsity in single-cell RNA sequencing, and the complex cell state pattern of expression within individual cell types. …”
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CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects by Vijay Sheri, Harikrishnan Mohan, Phanikanth Jogam, Anshu Alok, Gulab Khan Rohela, Baohong Zhang

“…To ensure specificity and avoiding off-target effects, we need to optimize various parameters such as target site, guide RNA design, and choosing right Cas variants. We outline the future prospects of CRISPR/Cas in cotton breeding, suggesting areas for further research and innovation. …”
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TIM‐4 increases the proportion of CD4+CD25+FOXP3+ regulatory T cells in the pancreatic ductal adenocarcinoma microenvironment by inhibiting IL‐6 secretion by Ziyao Wang, Zerong Xie, Yu Mou, Ruiman Geng, Chen Chen, Nengwen Ke

“…The influence of TIM‐4 overexpression on cell function was analyzed using RNA‐seq. Flow cytometry and ELISA were used for verification. …”
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蛋白酶体β5亚单位过表达对氧化环境下人晶状体上皮细胞的影响 by 张铁英, 柳夏林, 吴明星, 刘奕志

“…【结果】转染后用G418筛选3周后，获得PSMB5转染及空载体转染的G418抗性的细胞克隆。转染PSMB5基因的细胞PSMB5 mRNA表达强度明显增高．而空载体转染细胞与未转染细胞无显著差异：转染PSMB5基因的细胞的PSMB5蛋白表达也显著高于空载体转染细胞。…”
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RAB3GAP2 dysregulation in adult T-cell leukemia/lymphoma (ATLL) compared to acute lymphoblastic leukemia (ALL): a molecular perspective by Samira Pourrezaei, Arash Letafati, Ghazale Molaverdi, Mehdi Norouzi, Sayed-Hamidreza Mozhgani

“…Real-time quantitative PCR was then performed to quantify mRNA expression levels of SMC6, FANCM, EIF4H, WDR7, RAB3GAP2, and IFN α/β. …”
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Piperine regulates the circadian rhythms of hepatic clock gene expressions and gut microbiota in high-fat diet-induced obese rats by Weiyun Zhang, Chi-Tang Ho, Wenlian Wei, Jie Xiao, Muwen Lu

“…The gut microbial communities studied through 16S rRNA sequencing showed that PIP ameliorated the imbalanced microbiota and recovered the circadian rhythm of Lactobacillaceae, Desulfovibrionaceae, Paraprevotellaceae, and Lachnospiraceae. …”
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The role of HSPA14 in breast cancer: implications for tumorigenesis, immune response modulation, and personalized therapies by Ruipeng Zhao, Xiaocun Zhu, Wan Wei, Linlin Zhen

“…The data on HSPA14 levels and drug sensitivity were extracted from the CellMiner dataset. The mRNA expression of HSPA14 was validated through cell experiments.Results HSPA14 expression is elevated in breast cancer, which is associated with poor overall survival. …”
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Uptake of iron from ferrous fumarate can be mediated by clathrin-dependent endocytosis in Hutu-80 cells by Agata Tarczykowska, Per Malmberg, Nathalie Scheers

“…Here we propose that iron from the simple salt ferrous fumarate, a common iron supplement, can be absorbed by clathrin-mediated endocytosis. We used siRNA to silence DMT1 transporter expression, pharmacological inhibition of endocytosis, and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) to show that iron uptake from ferrous fumarate can be mediated by both transport via DMT1 and by clathrin-dependent endocytosis in Hutu-80 cells. …”
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