p104 Binds to Rac1 and Reduces Its Activity during Myotube Differentiation of C2C12 Cell by Ki Young Choi, Min Sup Lee, Young Jun Cho, Myong Ho Jeong, Seung Jin Han, Seung Hwan Hong

“…The overexpression of p104 enhanced myotube differentiation, whereas siRNA of p104 reversed this process. In this process, more Rac1 and CrkII were bound to increased p104. …”
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Reflections on COVID-19: A Literature Review of SARS-CoV-2 Testing by Chin Shern Lau, Helen M. L. Oh, Tar Choon Aw

“…Spike antibodies tracked the rise in antibodies after vaccination with mRNA vaccines and became invaluable in the assessment of vaccine response. …”
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Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants by Kishan Raj, Anup Singh, Tapas Chowdhury, S Gupta, Ravindra Soni

“…The isolated AMB-CD-1 was identified as Pseudomonas aeruginosa through the Sanger sequencing technique of the 16S rRNA gene. Results also revealed that the highest germination percentage, shoot weight and shoot length were found to be significantly superior in T4 (50% Sand + 50% Compost) whereas the highest chlorophyll content was found in T7 (25% Sand + 25% Soil + 50% Compost), and T1 (100% Sand) was effective in terms of root length.Conclusion Overall, the study concluded that Pseudomonas aeruginosa AMB-CD-1 might be a fast decomposer of paddy straw.…”
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Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues by Helen C. Fernandes, Ana F. Costa, Michelle A. R. Freitas, Almir S. Martins, Jorge L. Pesquero, Élida M. Rabelo, Maria A. Gomes

“…One Brazilian strain of E. histolytica in two conditions, able or not to cause lesions in experimental animals, was used. RNA from this strain, was used to study the differential expression of genes. 29 specific gene fragments differentially expressed in the virulent strain were selected. …”
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Transmission of potato spindle tuber viroid between <i>Phytophthora infestans</i> and host plants by O. S. Afanasenko, A. V. Khiutti, N. V. Mironenko, N. M. Lashina

“…Potato spindle tuber viroid (PSTVd) is a naked, circular, single-stranded RNA (356–363 nucleotides in length) which lacks any protein-coding sequences. …”
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Goat milk-based infant formula regulates intestinal barrier function and promotes the production of short-chain fatty acids by Qingxue Chen, Ting Cao, Hongwei Tang, Linyi Zhou, Yuxuan Zheng, Jinju Cheng, Bailiang Li, Song Wang

“…The results showed that goat milk-based IF is associated with a functional immune advantage, due to the rise in the levels of immune-related cytokines interleukin (IL)-2 and IL-10, decreased levels of intestinal permeability markers D-lactic acid and endotoxin, and increased mRNA levels of intestinal tight junction proteins occludin and claudin. …”
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Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer by Havina Nurul Aizah, Amel Yanis, Hirowati Ali, Tofrizal Tofrizal, Cimi Ilmiawati, Dessy Arisanty

“…Methods: This was an experimental study with a post-test-only control group design, including three groups with a total sample of 18 samples of Alzheimer RNA rat models. Group-1without AlCl3 and MSC-WJ; Group-2 with AlCl3; Group-3 AlCl3 and MSC-WJ. …”
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ANKRD22 participates in the proinflammatory activities of macrophages in the colon cancer tumor microenvironment by Xiaoying Wang, Keqing Yang, Bin Yang, Rui Wang, Yongliang Zhu, Tianhui Pan

“…A significant decrease in ANKRD22 expression in macrophages cocultured with colon cancer cell culture supernatant as well as in macrophages directly derived from colorectal cancer tissues was observed. Single-cell RNA sequencing, spatial transcriptomic studies, and subcutaneous xenograft experiments in mice revealed that Ankrd22 silencing altered the subtype distribution of macrophages, attenuated their proinflammatory activity, and enhanced their protumor activity. …”
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Positive feedback between arginine methylation of YAP and methionine transporter SLC43A2 drives anticancer drug resistance by Xia-Lu Hong, Chen-Kai Huang, Hui Qian, Chen-Hong Ding, Fang Liu, Huan-Yu Hong, Shu-Qing Liu, Si-Han Wu, Xin Zhang, Wei-Fen Xie

“…Methionine participates in cellular processes by converting to methyl donor for the methylation of DNA, RNA and protein. However, it remains unclear whether methionine affects drug resistance by influencing YAP activity. …”
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Ang-1/Tie2在大鼠体-肺分流肺动脉高压形成中的作用

“…【目的】 探讨Ang-1及其受体Tie2在体-肺分流肺动脉高压形成过程中的作用65377;【方法】 分流组20只大鼠行左颈总动脉-左颈外静脉分流手术,对照组16只大鼠行假手术65377;分别于术后4周和8周测量右心室收缩压,并对大鼠肺组织进行组织学分析,测量肺微细动脉壁厚度指数65377;通过实时荧光定量RT-PCR和Western Blot的方法,检测大鼠肺组织中Ang-1和Tie2的表达65377;同时,通过Western Blot检测caspase-3及激活型caspase-3的表达65377; 【结果】 与对照组相比,分流组大鼠术后第4周和第8周右心室收缩压明显升高65377;组织学分析发现,分流组大鼠肺微细动脉内膜内皮细胞增生肥大,血管壁厚度指数增加65377;在分流引起肺动脉高压形成的过程中,Ang-1和Tie2在mRNA及蛋白水平上均明显增加65377;与此同时,标志细胞调亡的激活型caspase-3的表达却明显下降65377; 【结论】 Ang-1和Tie2的表达与肺动脉高压紧密相关65377;Ang-1/Tie2系统可能通过抑制内皮细胞凋亡,促进体-肺分流肺动脉高压的形成65377;…”
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Astragaloside IV inhibits pathological functions of gastric cancer-associated fibroblasts through regulation of the HOXA6/ZBTB12 axis by Liu Haibo, Luo Shicheng, Sha Xiaofeng, Chen Zhiping, Yang Dongdong

“…HOXA6 silencing also induced the downregulation of ZBTB12 mRNA and protein in CAFs. Astragaloside IV was demonstrated to regulate the expression of ZBTB12 by mediating the transcriptional activity of HOXA6. …”
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The role of IFN-γ/CXCL10 axis in Mycoplasma pneumonia infection by Yanxia Zou, Feng Huang, Jiying Sun, Yidan Zheng, Ge Dai, Ting Wang, Canhong Zhu, Yongdong Yan, Renzheng Wang, Zhengrong Chen

“…In vitro, coculture with IFN-γ or activated CD4+ Th1 cells significantly promoted CXCL10 expressions in THP-1 derived macrophages, which was largely reversed by siRNA-mediated down regulation of STAT1. In addition, IFN-γ-stimulated macrophages greatly promoted the trans-migration of Th1 cells. our data show that Th1 cells-derived IFN-γ augments CXCL10 production in macrophages via the JAK-STAT1 pathway, which subsequently recruits more immune cells like Th1 cells into the infection sites, thereby constituting a positive feedback loop and aggravating the type I inflammatory responses in MPP patients.…”
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Mettl3-m6A-NPY axis governing neuron–microglia interaction regulates sleep amount of mice by Qihang Sun, Jinpiao Zhu, Xingsen Zhao, Xiaoli Huang, Wenzheng Qu, Xia Tang, Daqing Ma, Qiang Shu, Xuekun Li

“…In the present study, we showed that the deficiency of RNA m6A methyltransferase Mettl3 in excitatory neurons specifically induces microglia activation, neuroinflammation and neuronal loss in thalamus of mice. …”
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Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli by Gang Liu, Shuai Chen, Guiping Guan, Jun Tan, Naif A. Al-Dhabi, Hongbing Wang, Veeramuthu Duraipandiyan, Jun Fang

“…At day 7 after infection, the bacterial load in the jejunum and faeces was significantly lower in the COS group than that in the control group. Moreover, the mRNA and protein levels of IL-1β, IL-6, IL-17, IL-18, and TNF-α were significantly lower in the group of mice receiving the COS diet; also the jejunal production of toll-like receptor-4 (TLR-4) was suppressed in the COS group. …”
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Altered Hepatic Lipid Metabolism Contributes to Nonalcoholic Fatty Liver Disease in Leptin-Deficient Ob/Ob Mice by James W. Perfield, Laura C. Ortinau, R. Taylor Pickering, Meghan L. Ruebel, Grace M. Meers, R. Scott Rector

“…Ob/Ob mice also had greater liver weights, hepatic triglyceride (TG) content, and markers of de novo lipogenesis, including increased hepatic gene expression and protein content of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and stearoyl-CoA desaturase-1 (SCD-1), as well as elevated gene expression of PPARγ and SREBP-1c compared with WT mice. While hepatic mRNA levels for PGC-1α, PPARα, and TFAM were elevated in Ob/Ob mice, measures of mitochondrial function (β-HAD activity and complete (to CO2) and total mitochondrial palmitate oxidation) and mitochondrial OXPHOS protein subunits I, III, and V content were significantly reduced compared with WT animals. …”
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Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway by Song Yang, Lei Yao, Xiaolong Wang, Hao Sun, Chaogang Du, Chengpeng Song, Jingyu Fu, Yongjun Wu, Hongwu Huang, Chuansi Wang, Yongsen Wang, Yixiang Xie

“…The underlying mechanisms were detected by Western blotting assays and BMP-2 si-RNA silencing assay in vitro and in vivo. Results. …”
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Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc by Bin Chen, Jing Yu, Qianqian Wang, Yuanyuan Zhao, Li Sun, Changgen Xu, Xiangdong Zhao, Bo Shen, Mei Wang, Wenrong Xu, Wei Zhu

“…Moreover, we found that the c-Myc inhibitor JQ1 and c-Myc siRNA decreased the expression of c-Myc in hBM-MSC-CM-treated tumor cells in vitro. …”
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The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model by In-Su Park, Ji Hye Kim, Jun-Sang Bae, Dong-Kyu Kim, Ji-Hun Mo

“…Additionally, we found that the 10 mg T-MSCs-CM-treated group showed a significantly decreased IL-4 mRNA expression, compared to the (+) Con group. In the analysis of T cell receptor signal, the phosphorylation of MAP kinases, translocation of p65, and activation of NFAT1 were inhibited after T-MSCs-CM. …”
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Two Paediatric Patients with Encephalopathy and Concurrent COVID-19 Infection: Two Sides of the Same Coin? by Katerina Vraka, Dipak Ram, Siobhan West, Wei Yen Evelyn Chia, Praveen Kurup, Gayathri Subramanian, Hui Jeen Tan

“…Both patients had ADEM-like changes in their neuroimaging, negative SARS-CoV-2 RNA PCR in CSF, and paucity of PIMS-TS laboratory findings. …”
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Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways by Xiyao Pang, Ying Zhuang, Zehan Li, Shuanglin Jing, Qin Cai, Fengge Zhang, Changao Xue, Jinhua Yu

“…The expression of odonto/osteogenic markers was significantly upregulated in mRNA levels and protein levels. Moreover, intermittent treatment of PTH also increased phosphorylation of JNK and P38, and the differentiation was suppressed following the inhibition of JNK and P38 MAPK pathways. …”
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