Identification of deep intronic variants in junctional epidermolysis bullosa using genome sequencing and splicing assays by Fuying Chen, Ruoqu Wei, Yumeng Wang, Qiaoyu Cao, Jianbo Wang, Chenfei Wang, Dingjin Yao, Zhirong Yao, Cheng Ni, Ming Li

“…Our results emphasize the importance of selecting the right cell line for mRNA analysis.…”
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Terminalia catappa Extract Palliates Redox Imbalance and Inflammation in Diabetic Rats by Upregulating Nrf-2 Gene by Franklyn Nonso Iheagwam, Gaber El-Saber Batiha, Olubanke Olujoke Ogunlana, Shalom Nwodo Chinedu

“…TCA treatment significantly (p<0.05) modulated systemic diabetic-induced oxidative stress and inflammation, mRNA expression dysregulation, and dysregulated macromolecule metabolism. …”
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Endometrial polyps with bizarre stromal cells: a Benign or a low-grade lesion? by Musen Wang, Fei Gao, Hongkai Zhang, Wenxin Zheng

“…The histopathology mirrored the initial findings, showing no myometrial invasion, prompting a re-evaluation of the presumed benign nature of the polyp given its rapid growth. Remarkably, RNA sequencing analysis of the polyp detected a JAK2::NFIB gene fusion, a novel finding for endometrial polyps with atypical stromal cells, the clinical implications of which remain to be elucidated. …”
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P-Selectin Targeted Dexamethasone-Loaded Lipid Nanoemulsions: A Novel Therapy to Reduce Vascular Inflammation by Viorel Simion, Cristina Ana Constantinescu, Daniela Stan, Mariana Deleanu, Monica Madalina Tucureanu, Elena Butoi, Ileana Manduteanu, Maya Simionescu, Manuela Calin

“…Given intravenously in mice with acute inflammation, PLN-Dex accumulated at a significant high level in the lungs (compared to nontargeted nanoemulsions) and significantly reduced mRNA expression level of key proinflammatory cytokines such as IL-1β, IL-6, and MCP-1. …”
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Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment by Selvaraj Jeraldin Nisha, Ganapathi Uma, Ramamoorthy Sathishkumar, Vincent Samuel Gnana Prakash, Rimal Isaac, Thavasimuthu Citarasu

“…The selected strain was identified as Streptomyces sp CMSTAAHL-4 by 16S rRNA sequencing. The media optimization for secondary metabolites production was performed by One-Variable at a Time and Response Surface Methodology-Central Composite Design. …”
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Impact of Endurance Exercise Training on Biomarkers of Aortic Endothelial Damage in Diabetic Rats by Mahtab Fouladi, Maryam Mahmoudabady, Zahra Gholamnezhad, Sadegh Shabab, Saeed Niazmand, Hossein Salmani

“…After 6 weeks, biochemical parameters in serum and oxidative stress markers and mRNA expression of endothelial nitric oxide synthase (eNOS), lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), and intercellular adhesion molecule-1 (ICAM-1) in aorta tissue were assessed. …”
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Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress by Qiao Wang, Lijun Meng, Zechao Qu, Huan Su, Jiemeng Tao, Peng Lu, Chenfeng Hua, Jinbang Wang, Jianfeng Zhang, Peijian Cao, Jingjing Jin

“…Through expression pattern analysis of public RNA-seq datasets and qRT-PCR experiments, it was observed that many NtDnaJAs displayed tissue-specific expression and might play significant roles in different biotic and abiotic stresses. …”
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Limosilactobacillus reuteri fermented brown rice alleviates anxiety improves cognition and modulates gut microbiota in stressed mice by Akanksha Tyagi, Yu-Yeong Choi, Lingyue Shan, Annadurai Vinothkanna, Eun-Seok Lee, Ramachandran Chelliah, Kaliyan Barathikannan, Sivakumar Thasma Raman, Se Jin Park, Ai-Qun Jia, Geun Pyo Choi, Deog Hwan Oh

“…A four-week FBR regimen reduced corticosterone, restored neurotransmitters like gamma-aminobutyric acid (GABA) and serotonin, and improved anxiety-related behaviors. Metagenomic (16S rRNA) and metabolomic analyses revealed enhanced amino acid metabolism, energy metabolism, and short-chain fatty acid (SCFA) production in FBR-treated mice. …”
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Regulation of Hypoxia-Induced Cell Death in Human Tenocytes by Min Liang, Hannah R. Cornell, Nasim Zargar Baboldashti, Mark S. Thompson, Andrew J. Carr, Philippa A. Hulley

“…Hypoxia markedly upregulated VEGF-A mRNA, followed by increased VEGF protein secretion. …”
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Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging by Yaqun Jiang MD, Yongkang Gai PhD, Yu Long MD, Qingyao Liu PhD, Chunbao Liu MD, PhD, Yongxue Zhang MD, Xiaoli Lan MD, PhD

“…It has been reported that dysregulation of microRNA-155 expression and function is associated with tumorigenesis, growth, tumor subtypes, invasion, and poor survival rates. …”
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Ya That Somdun improves hepatic steatosis in hyperlipidemic rats by Suchittra Samuhasaneeto, Gorawit Yusakul, Chuchard Punsawad, Kingkan Bunluepuech

“…YTS treatment (100 mg/kg BW) downregulated sterol regulatory element binding protein 1c (SREBP-1c) mRNA expression and alleviated hepatic steatosis. …”
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Codon optimized influenza H1 HA sequence but not CTLA-4 targeting of HA antigen to enhance the efficacy of DNA vaccines in an animal model by Dito Anurogo, Chia-Yuan Chen, Chu-Chi Lin, Jeanne Adiwinata Pawitan, Daniel W. Qiu, J. Timothy Qiu

“…The study findings revealed that the codon-optimized HA gene (pcHA) led to increased protein synthesis, as evidenced by elevated mRNA levels. Codon optimization also significantly bolstered both cellular and humoral immune responses. …”
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CRYβB2 alters cell adhesion to promote invasion in a triple-negative breast cancer cell line by Amr A. Waly, London Harper, Jodie M. Fleming, Lindsey M. Costantini

“…A quantitative 3D invasion assay demonstrated that CRYβB2 overexpression significantly enhanced invasion (median invasion %; SUM159 = 0.14 and SUM159 + CRYβB2 = 0.33). RNA sequencing analysis indicated that CRYβB2 overexpression modulated cell-cell adhesion and extracellular matrix organization pathways, which are critical to invasion of cancer cells. …”
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Characterization of Microbiota in Endometrial Fluid and Vaginal Secretions in Infertile Women with Repeated Implantation Failure by Kotaro Kitaya, Yoko Nagai, Wataru Arai, Yoshiyuki Sakuraba, Tomomoto Ishikawa

“…Extracted genomic DNA was pyrosequenced for the V4 region of 16S ribosomal RNA using a next-generation sequencer. The EF microbiota had higher α-diversity and broader bacterial species than the VS microbiota both in the RIF and control groups. …”
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Potential biomarkers for predicting the risk of thyroid cancer in immunosenescence: a population-based and externally validated multi omics study by Qian Li, Yuanyuan Zhao, Jiawei Yan, Chao He

“…Finally, complementary results were obtained from an independent cohort analyzed at Chongqing Medical University Yongchuan Hospital and Bulk-RNA seq from GEO database.ResultsCausal analysis suggests that DNA methylation age acceleration as measured by the Hannum method increases the risk of thyroid cancer (OR: 1.126, 95% CI: 1.002-1.265, P=0.046). …”
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Emodin Ameliorates Intestinal Dysfunction by Maintaining Intestinal Barrier Integrity and Modulating the Microbiota in Septic Mice by Mina Zhang, Bo Lian, Rui Zhang, Yuhong Guo, Jingxia Zhao, Shasha He, Yunjing Bai, Ning Wang, Yan Lin, Xuerui Wang, Qingquan Liu, Xiaolong Xu

“…Bioinformatic analysis based on 16s rRNA gene sequencing illustrated that emodin treatment significantly increased the alpha- and beta-diversity of gut microbiota in septic mice. …”
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NEIL3 Mediates Lung Cancer Progression and Modulates PI3K/AKT/mTOR Signaling: A Potential Therapeutic Target by Hongbo Huang, Qingwang Hua

“…Quantitative real-time PCR (qRT-PCR) and western blot analyses were utilized to detect RNA and protein levels. Biological differences between groups were determined by gene set enrichment analysis (GSEA). …”
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FeLV-induced feline leukemia as a natural model for leukemia pathophysiology study (review) by T. V. Tabakaeva, A. V. Tsybulsky, K. A. Baranchugova, I. V. Galkina, M. Yu. Shchelkanov

“…Feline leukemia etiological agent is a feline leukemia virus (FeLV), having single-stranded RNA genomesurrounded with icosahedral capsidformedbyp27 capsid protein monomers. …”
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Liraglutide Promotes Osteoblastic Differentiation in MC3T3-E1 Cells by ERK5 Pathway by Yue Sun, Yuzhen Liang, Zhengming Li, Ning Xia

“…Moreover, the activity levels of alkaline phosphatase and the number of mineralized nodules in MC3T3-E1 cells were significantly increased following treatment with 100 nM liraglutide. The mRNA and protein levels of Col-1, OPG, and OCN in MC3T3-E1 cells were also markedly increased following 100 nM liraglutide treatment compared with those of the control group. …”
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Identifying Key Biomarkers Related to Immune Response in the Progression of Diabetic Kidney Disease: Mendelian Randomization Combined With Comprehensive Transcriptomics and Single-... by Hu M, Deng Y, Bai Y, Zhang J, Shen X, Shen L, Zhou L

“…Finally, we validated the mRNA expression of five key genes in the DKD mice model using reverse transcription quantitative PCR (RT-qPCR).Results: Five characteristic genes, known as protein kinase B beta (AKT2), interleukin-2 receptor beta (IL2RB), neurexin 3(NRXN3), slit homolog 3(SLIT3), and TATA box binding protein like protein 1 (TBPL1), demonstrated causal relationships with DKD. …”
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