Gender Variations in the Oral Microbiomes of Elderly Patients with Initial Periodontitis by Jie Zhao, Ying-Hui Zhou, Ya-Qiong Zhao, Yao Feng, Fei Yan, Zheng-Rong Gao, Qin Ye, Yun Chen, Qiong Liu, Li Tan, Shao-Hui Zhang, Jing Hu, Marie Aimee Dusenge, Yun-Zhi Feng, Yue Guo

“…Twelve males and twenty females (range 50–68 years old) with initial periodontitis provided subgingival plaque samples. 16S rRNA gene sequencing, QIIME-based data processing, and statistical analyses were carried out using several different analytical approaches to detect differences in the oral microbiome between the two groups. …”
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Sinistroporomonorchis bolini n. sp. (Trematoda: Monorchiidae) from the Ocellated killifish, Floridichthys polyommus (Cyprinodontidae) in the Yucatán Peninsula, Mexico based on an i... by Leopoldo Andrade-Gómez, Juan F. Espínola-Novelo, Brenda Solórzano-García, Gerardo Pérez-Ponce de León

“…In addition, sequences of the 28S of large subunit of nuclear ribosomal RNA and cox1 of the mitochondrial DNA were obtained. …”
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内蒙古赤峰地区东北马鹿（Cervus elaphus xanthopygus）冬季肠道微生物多样性研究 by 郭金昊, 袁子奥, 张玮琪, 张明海

“…基于16S rRNA基因测序技术对24份东北马鹿冬季粪便中细菌的结构和多样性进行分析，试图揭示东北马鹿冬季肠道微生态，为该物种保护提供新的见解。…”
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Comprehensive analysis pinpoints CCNA2 as a prognostic and immunological biomarker in non-small cell lung cancer by Liming Zhang, Shaoqiang Wang, Lina Wang

“…Clinicopathological correlations were assessed via the Human Protein Atlas (HPA) and UALCAN databases. qRT-PCR and immunohistochemistry (IHC) were performed to validate CCNA2 mRNA and protein levels. Loss-of-function assays in lung cancer cell lines evaluated the biological role of CCNA2. …”
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Analysis of whole-genome binding patterns of GAGA and CNC transcription factors during Drosophila melanogaster development by I. I. Brusentsov, D. A. Karagodin, E. M. Baricheva, T. I. Merkulova

“…In order to select a set of genes potentially co-regulated by GAGA and CNC, the study of their co-binding in annotated regulatory regions (promoter areas and segments corresponding to the 5’-UTR and 3’-UTR of mRNA) was performed. The results obtained clearly demonstrated that the sets of genes characterized by co-binding of both factors vary greatly at different stages. …”
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SSR-based evaluation of genetic diversity in populations of Agriophyllum squarrosum L. and Agriophyllum minus Fisch. & Mey. collected in South-East Kazakhstan by Y. Genievskaya, D. Karelova, S. Abugalieva, P. Zhao, G. Chen, Y. Turuspekov

“…& Mey. However, a recent RNA-sequencing project in A. squarrosum has proposed a large set of hypothetical SSR (simple sequence repeat) markers. …”
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The interaction of Adipose Derived Stem Cells and Breast Cancer by Natasha Cunningham, Ava O'Meara, Cathy L. Brougham

“…Findings: Upregulation of miRNA expression all miR-21 co-culture samples, miR-222 T47D co-culture, and both miR-146, and miR-221’s SKBR3 BC co-culture cell line. …”
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Chitosan nanoencapsulation of Turbinaria triquetra metabolites in the management of podocyturia in nephrotoxic rats by Abdullah Abdel-Aal, Abdel-Aziz A. F., Zakaria El-khayat, Nadia Mohamed, Merit Rostom, Emad Tolba, Nihal Galal El-Din Shams El-Din, Walaa S. A. Mettwally, Abdelhamid Aly Hamdy

“…Serum urea, creatinine, creatinine clearance, renal malondialdehyde, nitric oxide, paraoxonase 1, renal nephrin, and podocin, and their renal mRNA gene expressions, as well as urinary nephrin and podocin were determined. …”
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Deciphering the nature and statistical optimization of antimicrobial metabolites of two endophytic bacilli by Raghda S. Isleem, Ahmed M. Eid, Saad El-Din Hassan, Khaled M. Aboshanab, Ghadir S. El-Housseiny

“…Two bacterial isolates, C6 and C11, were found to have a broad-spectrum antagonistic effect against four standard microbial strains and were molecularly identified using 16 S ribosomal RNA sequence analysis and deposited in a local culture collection as B. velezensis CCASU-C6, and B. subtilis CCASU-C11, respectively. …”
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CD36-Mediated Lipid Accumulation and Activation of NLRP3 Inflammasome Lead to Podocyte Injury in Obesity-Related Glomerulopathy by Jing Zhao, Hong-liang Rui, Min Yang, Li-jun Sun, Hong-rui Dong, Hong Cheng

“…In addition, after blocking CD36 with inhibitor sulfo-N-succinimidyl oleate (SSO) or CD36 siRNA, activation of NLRP3 inflammasome and release of IL-1β are downregulated, and podocyte injury was alleviated. …”
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Beneficial Effects of Probiotic Treatment on Gut Microbiota in Very Low Birth Weight Infants by Yue-feng Li, Chuan-rui Zhu, Xue-lei Gong, Hui-ling Li, Li-kuan Xiong, Ke-jian Wang, Guo-Sheng Liu

“…Finally, 32 fecal specimens extracted from 16 preterm VLBW infants were qualified and analyzed using 16S rRNA gene sequencing. The primary outcome was to evaluate the change of gut microbiota in VLBW infants after combined probiotic supplement. …”
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Evaluation of bone development and organs in rat fetuses exposed to tartrazine by Osman Öztürk, Sümeyye Uçar, Züleyha Doğanyiğit, Aslı Okan Oflamaz, Evrim Suna Arıkan, Şükrü Ateş, Seher Yılmaz

“…It was observed that the mRNA levels of IL-6, IL-1β, TNF-α and TRPM2 genes in the livers of the fetuses changed compared to the control group (p < 0.05). …”
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SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection by Alexsia Richards, Andrew S. Khalil, Max Friesen, Troy W. Whitfield, Xinlei Gao, Tenzin Lungjangwa, Roger D. Kamm, Zhengpeng Wan, Lee Gehrke, David Mooney, Rudolf Jaenisch

“…Our results demonstrate that perivascular cells, particularly SMCs, are a susceptible vascular target for SARS-CoV-2 infection. Utilizing RNA sequencing, we characterize the transcriptomic changes accompanying SARS-CoV-2 infection of SMCs, PCs, and ECs. …”
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Integrative analysis of Ewing’s sarcoma reveals that the MIF-CD74 axis is a target for immunotherapy by Fangzhou He, Jiuhui Xu, Fanwei Zeng, Boyang Wang, Yi Yang, Jie Xu, Xin Sun, Tingting Ren, Xiaodong Tang

“…Methods Since novel immunotherapies may address this need, we performed an integrative analysis involving single-cell RNA sequencing, cell function experiments, and humanized models to dissect the immunoregulatory interactions in EwS and identify strategies for optimizing immunotherapeutic efficacy. …”
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Laxative Effect of Lacticaseibacillus rhamnosus Glory LG12: from the Perspective of the Stem Cell Factor/C-kit Signaling Pathway by XIANG Fangqin, LI Yuxing, LI Bailiang

“…In addition, both treatments elevated the serum levels of motilin (MTL), gastrin (Gas), substance P (SP), and interleukin 10 (IL-10), reduced the levels of endothelin-1 (ET-1), somatostatin (SS), vasoactive intestinal peptide (VIP), and IL-6, up-regulated the relative mRNA expression of SCF and C-kit in colonic tissue was (P < 0.01, P < 0.001), and restored damaged colonic barrier. …”
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Antimicrobial activity of Lactobacillus spp. isolated from fermented foods and their inhibitory effect against foodborne pathogens by Athraa Oudah Hussein, Khalida Khalil, Nurul Aqilah Mohd Zaini, Ahmed Khassaf Al Atya, Wan Syaidatul Aqma

“…They were then identified by morphological, physiological and biochemical characteristics and confirmed by 16S rRNA gene sequencing. Culture supernatants from seven lactobacilli isolates showed varying inhibitory activities. …”
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PCR-based detection of Botryosphaeria canker pathogens in fig trees by Mahdiyeh Ghaedi, Zeinab Bolboli, Hamed Negahban, Reza Mostowfizadeh-Ghalamfarsa

“…In our study, we developed a PCR assay using three carefully selected primer pairs based on sequence differences in two protein-coding genes, β-tubulin and RNA polymerase II. The species-specific primers TUB-Bd1 (specific for Botryosphaeria dothidea), TUB-Np1 (specific for Neofusicoccum parvum), and RPB-Nd2 (specific for Neoscytalidium dimidiatum) effectively amplified target gene sequences in pure cultures and infected tissue samples using PCR and nested PCR conditions. …”
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A Nonsynonymous Single-nucleotide Polymorphism in SLC24A5 Regulates Feather Pigment Deposition in Chinese Yellow Quail by Xiaohui Zhang, Fanghu Wu, Yanxia Qi, Yuanyuan Shang, Lingyun Fan, Yifei Wang

“…Mutant individuals had significantly lower total melanin content in the feathers and tyrosinase activity in dorsal skin, in spite of no significant difference in SLC24A5 mRNA expression in the same tissues. This study indicates that the g.8884145A/G mutation reduced tyrosinase activity by affecting the function of the SLC24A5 protein, which in turn decreased melanin content of down feathers in Chinese yellow quail.…”
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CD226 Deficiency Alleviates Murine Allergic Rhinitis by Suppressing Group 2 Innate Lymphoid Cell Responses by Yang Xie, Yuan Zhang, Tianxiao Zhu, Jingchang Ma, Chujun Duan, Lu Yang, Tingting Wang, Ran Zhuang, Ka Bian, Lianjun Lu

“…Hematoxylin and eosin and periodic acid–Schiff staining and quantitative real-time PCR methods were used to determine eosinophils, goblet cells, and ILC2-associated mRNA levels in the nasal tissues of mice. CD226 levels on ILC2s were detected using flow cytometry, and an immunofluorescence double staining assay was employed to determine the number of ILC2s in the nasal mucosa. …”
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Tumor-derived colorectal cancer organoids induce a unique Treg cell population by directing CD4+ T cell differentiation by Sonia Aristin Revilla, Cynthia L. Frederiks, Stefan Prekovic, Enric Mocholi, Onno Kranenburg, Paul J. Coffer

“…Co-culture resulted in a significant increase in Treg cell numbers. RNA-sequencing identified a distinct transcriptional profile of CRC organoid-induced Treg cells, with upregulation of genes associated with CRC Treg cells in vivo. …”
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