YTHDF1-mediated m6A modification of GBP4 promotes M1 macrophage polarization in acute lung injury by Fengan Cao, Shilei Wang, Qiuyue Tan, Junna Hou, Yunlu Li, Wentao Ma, Shilong Zhao, Jing Gao

“…YTHDF1 was found to enhance GBP4 expression by recognizing m6A sites on its mRNA, which was linked to reduced inflammation in MLE-12 cells upon YTHDF1 knockdown. …”
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Cardiovascular Risk in Systemic Autoimmune Diseases: Epigenetic Mechanisms of Immune Regulatory Functions by Chary López-Pedrera, Carlos Pérez-Sánchez, Manuel Ramos-Casals, Monica Santos-Gonzalez, Antonio Rodriguez-Ariza, Ma José Cuadrado

“…Epigenetic regulatory mechanisms comprise DNA methylation, histone modifications, and microRNA activity, all of which act upon gene and protein expression levels. …”
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Molecular reassessment of diaporthalean fungi associated with strawberry, including the leaf blight fungus, Paraphomopsis obscurans gen. et comb. nov. (Melanconiellaceae) by Dhanushka Udayanga, Shaneya D. Miriyagalla, Dimuthu S. Manamgoda, Kim S. Lewers, Alain Gardiennet, Lisa A. Castlebury

“…Combined analyses of four nuclear loci, 28S ribosomal DNA/large subunit rDNA (LSU), ribosomal internal transcribed spacers 1 and 2 with 5.8S ribosomal DNA (ITS), partial sequences of second largest subunit of RNA polymerase II (RPB2) and translation elongation factor 1-α (TEF1), were used to reconstruct a phylogeny for these pathogens. …”
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Phillyrin ameliorates DSS-induced colitis in mice via modulating the gut microbiota and inhibiting the NF-κB/MLCK pathway by Tong Li, Guiqiu Hu, Shoupeng Fu, Di Qin, Zheyu Song

“…This mechanism results in the preservation of the integrity of the intestinal barrier. The outcomes of 16S rRNA sequencing suggest that PHY (50 mg/kg) augmented the relative abundance of certain probiotic strains, including Lactobacillaceae and Lachnospiraceae. …”
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Object-Place Recognition Learning Triggers Rapid Induction of Plasticity-Related Immediate Early Genes and Synaptic Proteins in the Rat Dentate Gyrus by Jonathan Soulé, Zsuzsa Penke, Tambudzai Kanhema, Maria Nordheim Alme, Serge Laroche, Clive R. Bramham

“…RT-PCR analysis from dentate gyrus tissue collected shortly after training did not reveal learning-specific changes in Arc mRNA expression. In situ hybridization and immunohistochemistry were therefore used to assess possible sparse effects on gene expression. …”
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GPR55 in the tumor microenvironment of pancreatic cancer controls tumorigenesis by Dušica Ristić, Thomas Bärnthaler, Eva Gruden, Melanie Kienzl, Laura Danner, Karolina Herceg, Arailym Sarsembayeva, Julia Kargl, Rudolf Schicho

“…ResultsDeficiency of GPR55 in the TME led to reduced tumor weight and volume, and altered the immune cell composition of tumors, favoring an anti-tumorigenic environment by increasing the number of CD3+ T cells, particularly CD8+ T cells, and the expression of PDL1 on macrophages. RNA-seq pathway analysis revealed higher T cell activity in KPCY55 tumors of GPR55 KO vs. …”
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TEC-mediated tRF-31R9J regulates histone lactylation and acetylation by HDAC1 to suppress hepatocyte ferroptosis and improve non-alcoholic steatohepatitis by Juanjuan Zhu, Xian Wu, Mao Mu, Quan Zhang, Xueke Zhao

“…Abstract Background Tectorigenin (TEC) is a monomer of anthocyanin, which we found exhibits hepatoprotective effects. tRNA-derived fragments (tRFs) and ferroptosis play important roles in the pathogenesis of non-alcoholic steatohepatitis (NASH). …”
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Acid sphingomyelinase modulates anxiety-like behavior likely through toll-like receptor signaling pathway by Huiqi Yuan, Yanan Xu, Hailun Jiang, Meizhu Jiang, Luofei Zhang, Shifeng Wei, Cao Li, Zhigang Zhao

“…In the present study, we employed Asm-knockout (Asm KO) mice to investigate the association between Asm and anxiety using behavioral tests, RNA sequencing, q-PCR, immunohistochemical staining, and other methods. …”
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Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway by Min Liu, Rui Chen, Yunxuan Xu, Jiawen Zheng, Min Wang, Ping Wang

“…Further analyses revealed that microRNA-141-3p (miR-141-3p) was expressed at relatively high levels in PDLSC-Exos and was instrumental in PDLSC-Exo-mediated restoration by downregulating Kelch-like ECH-associated protein 1 (KEAP1), which is a negative regulator of NRF2 expression. …”
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Fungal planktonic community related to salinity and temperature in an oligotrophic sea by Ashwag A. Asseri, Alexandra Coello-Camba, Susana Agustí

“…We collected water samples from the photic layer at six sites along the Red Sea basin and analyzed the fungal community by targeting the 28S rRNA gene. Our results showed that Chytridiomycota dominated these communities, accounting for 85% of reads, followed by members of the divisions Basidiomycota (4.7%) and Cryptomycota (4.13%). …”
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Role of silent mutations in KRAS-mutant tumors by Jun Lu, Chao Zhou, Feng Pan, Hongyu Liu, Haohua Jiang, Hua Zhong, Baohui Han, Xiangxiang Pan

“…Silent mutations, which do not alter protein sequences but can affect RNA stability and translational efficiency, pose intriguing questions regarding their contribution to tumor biology. …”
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Resveratrol, a food-derived polyphenol, promotes Melanosomal degradation in skin fibroblasts through coordinated activation of autophagy, lysosomal, and antioxidant pathways by Saki Okamoto, Saya Kakimaru, Mayuko Koreishi, Mika Sakamoto, Yoshimasa Nakamura, Hideya Ando, Yoshio Tsujino, Ayano Satoh

“…At concentrations of 25–50 μM, resveratrol increased autophagy as measured by microtubule-associated protein 1A/1B-light chain 3 (LC3)-II/LC3-I ratio and enhanced lysosomal activity as assessed by a lysosomal activity reporter system. RNA sequencing revealed upregulation of lysosomal and autophagy-related genes, including cathepsins. …”
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Two Imported Malaria Cases with Delayed Response to Treatment in Hatay by Tuğba Kaya, Mehmet Çabalak, Gülnaz Çulha, Ceren Ünal

“…In the SD-Pf/Pan test, bands were obtained for both P.f and Pan; while in the SD-Pf/Pv test, a band was obtained for P.f. The P. falciparum18S rRNA gene was detected using real-time polymerase chain reaction. …”
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A KIF20A-based thermosensitive hydrogel vaccine effectively potentiates immune checkpoint blockade therapy for hepatocellular carcinoma by Xingyang Zhao, Feichao Xuan, Zirong Li, Xiangyi Yin, Xiaojun Zeng, Jiali Chen, Chihua Fang

“…This study confirmed KIF20A as a promising immunogenic antigen through transcriptomic mRNA sequencing analysis in the context of HCC. Therefore, we developed a thermosensitive hydrogel vaccine formulation (K/RLip@Gel) to optimize antigen delivery while enabling sustained in vivo release. …”
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Characterization and molecular biological identification of tick species in Nanjing city by Luyao SUN, Xuemin CHEN, Bingdong SONG, Yan ZHANG, Yanqun SUN, Yue MIN, Shougang ZHANG

“…The ticks were identified by morphological classification and molecular biological identification based on 16S rRNA gene. ResultsA total of 1 523 ticks were collected, including 993 Haemaphysalis longicornis (65.20% of all ticks collected), 529 Haemaphysalis flava (34.73%), and one Haemaphysalis phasiana (0.07%). …”
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Endogenous peptide CBDP1 inhibits clear cell renal cell carcinoma progression by targeting USP5/YTHDF2/TRPM5 axis by Yang Zhang, Wei Zhu, Ruijie Tao, Weijian Li, Chunming Jiang, Xiang Yan

“…The upregulated YTHDF2 then binds to TRPM3 mRNA and promotes its degradation, ultimately reducing TRPM3 expression levels. …”
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Dengue Infection and Its Relationship with Evans Syndrome: A Pediatric Case by Ivan Jose Ardila Gomez, Pilar Pérez López, Mónika Rocío Hernández Carreño, Juan Camilo Barrios Torres

“…Dengue is a single-stranded RNA virus belonging to the Flaviviridae family. …”
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Antibacterial Potential of Crude Extracts from <i>Cylindrospermum alatosporum</i> NR125682 and <i>Loriellopsis cavernicola</i> NR117881 by Albert Olufemi Ikhane, Foluso Oluwagbemiga Osunsanmi, Rebamang Anthony Mosa, Andrew Rowland Opoku

“…The cyanobacteria were identified through 16S rRNA sequencing. Crude extracts were sequentially prepared using hexane, dichloromethane, and ethanol consistently. …”
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云南省发现越南趾沟蛙 by 杨 云 刘小龙 饶静秋 贺艳红 韩克国 王 剑 袁智勇

“…根据形态特征比较发现,该蛙皮肤光滑,背部褐色,肩上方有“人”形疣粒,体背后部有小疣粒,体腹呈浅黄色,胸、腹部有深色云斑,指端略膨大且无沟,后肢细长,胫长超过体长之半,跗褶部位有小疣,趾端呈小吸盘状且腹侧具沟,趾间全蹼,四肢背面有深褐色横纹,与采集自越南林同省郎边高原(Langbian Plateau,Lam Dong,Vietnam,模式产地)的越南趾沟蛙(<i>Rana johnsi</i>)形态鉴定特征基本一致,仅体型大小稍有差异。基于16S rRNA 基因片段的系统发育分析表明,该标本与越南趾沟蛙的样本构成单支系,且遗传距离较小(<i>p</i>-distance为0.8%),确定该标本为云南省两栖纲(Amphibia)分布新纪录物种。…”
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Effects of exogenous insulin supplementation on lipid metabolism in peripartum obese dairy cows by Yuanyin Guo, Yihan Zhao, Ziang Wei, Jie Cao

“…Blood samples were collected for biochemical indicators and subcutaneous adipose tissue was collected for paraffin-embedding and sectioning and RNA sequencing analysis. The results showed that insulin supplementation postpartum reduced concentrations of NEFA and BHBA as well as BCS loss, but did not affect glucose. …”
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