Effect of age and sex differences on the abundances of neuronal, glial, and endothelial cells in non-diseased brain tissue. by Arttu Autio-Kimura, Tapio Nevalainen, Mikko Hurme

“…The relative abundance of excitatory and inhibitory neurons, astrocytes, oligodendrocytes, microglia, and endothelial cells was estimated from the RNA sequencing data using a deconvolution-based analysis. …”
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Lnc-DC promotes estrogen independent growth and tamoxifen resistance in breast cancer by Wan-Xin Peng, Pratirodh Koirala, Huaixiang Zhou, Jiahong Jiang, Ziqiang Zhang, Liu Yang, Yin-Yuan Mo

“…Using a CRISPR/Cas9-based SAM (synergistic activation mediator) library against a focus group of lncRNAs, we identify Lnc-DC as a candidate lncRNA. Further analysis suggests that Lnc-DC is able to reduce tamoxifen-induced apoptosis by upregulation of anti-apoptotic genes such as Bcl2 and Bcl-xL. …”
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Decoding the aroma of Rosa canina L.: Chemical composition and gene expression. by Parisa Jariani, Ali-Akbar Shahnejat-Bushehri, Roohangiz Naderi, Meisam Zargar, Mohammad Reza Naghavi

“…<h4>Methods</h4>Essential oils (EOs) were extracted from petals at five developmental stages (S1-S5) and analyzed using Gas Chromatography-Mass Spectrometry (GC-MS), identifying 20 distinct compounds. RNA isolation and quantitative real-time PCR (qRT-PCR) analysis were performed to assess gene expression.…”
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Inhibition of GSK3β is synthetic lethal with FHIT loss in lung cancer by blocking homologous recombination repair by Shishi Tao, Yue Pu, Eun Ju Yang, Guowen Ren, Changxiang Shi, Li-Jie Chen, Liang Chen, Joong Sup Shim

“…Pharmacological inhibition or siRNA depletion of GSK3β selectively suppressed the growth of FHIT-deficient lung cancer tumors in vitro and in animal models. …”
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SLC35A2 is a novel prognostic biomarker and promotes cell proliferation and metastasis via Wnt/β-catenin/EMT signaling pathway in breast cancer by Rushu Yan, Tianwen Chen

“…We collected 11 pairs of BC tissues and normal specimens, obtaining clinical information from 1,118 BC patients through RNA sequencing analysis. Different BC cell lines were used in experiments, and the roles of SLC35A2 in cell proliferation, invasion, and migration was assessed through gene silencing and functional assays. …”
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Identifikasi Ekspresi Gen Myc pada Subkultur MCF-7 Breast Cancer Cell Line dengan Sel Punca by Yuastika Puspita Sari, Hirowati Ali, Aswiyanti Asri

“…Kemudian, dilakukan isolasi RNA, sintesis cDNA, dan ekspresi Myc diperiksa menggunakan PCR dan elektroforesis. …”
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A Reduction in Selenoprotein S Amplifies the Inflammatory Profile of Fast-Twitch Skeletal Muscle in the mdx Dystrophic Mouse by Craig Robert Wright, Giselle Larissa Allsopp, Alex Bernard Addinsall, Natasha Lee McRae, Sofianos Andrikopoulos, Nicole Stupka

“…In the extensor digitorum longus (EDL) muscles, mRNA expression of monocyte chemoattractant protein 1 (Mcp-1) (P=0.034), macrophage marker F4/80 (P=0.030), and transforming growth factor-β1 (Tgf-β1) (P=0.056) were increased in mdx:Seps1−/+ mice. …”
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Targeting AURKA with multifunctional nanoparticles in CRPC therapy by Bin Deng, Binghu Ke, Qixing Tian, Yukui Gao, Qiliang Zhai, Wenqiang Zhang

“…Bioinformatics analysis of the Cancer Genome Atlas-prostate adenocarcinoma (TCGA-PRAD) dataset revealed overexpression of AURKA in PCa, correlating with poor clinical outcomes. Single-cell RNA sequencing data from the GEO database showed a significant reduction in immune cells in CRPC. …”
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The Venus score for the assessment of the quality and trustworthiness of biomedical datasets by Davide Chicco, Alessandro Fabris, Giuseppe Jurman

“…We apply the Venus score to twelve datasets from multiple subdomains, including electronic health records, medical imaging, microarray and bulk RNA-seq gene expression, cheminformatics, physiologic electrogram signals, and medical text. …”
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An annotated high-content fluorescence microscopy dataset with EGFP-Galectin-3-stained cells and manually labelled outlineszenodo by Salma Kazemi Rashed, Malou Arvidsson, Rafsan Ahmed, Sonja Aits

“…Images were acquired on a Thermo Fischer CX7 high-content imaging system at 20x magnification created as part of an RNA interference screen with a modified U2OS osteosarcoma cell line. …”
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LongReadSum: A fast and flexible quality control and signal summarization tool for long-read sequencing data by Jonathan Elliot Perdomo, Mian Umair Ahsan, Qian Liu, Li Fang, Kai Wang

“…We also demonstrate a few examples using LongReadSum to analyze cDNA sequencing, direct RNA sequencing, ONT reduced representation methylation sequencing (RRMS), and whole genome sequencing (WGS) data.…”
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SESN2 ablation weakens exercise benefits on resilience of gut microbiota following high-fat diet consumption in mice by Chunxia Yu, Peng Zhang, Sujuan Liu, Yanmei Niu, Li Fu

“…Fecal microbiota composition and function were assessed by 16S rRNA sequencing. The sequencing results showed that SESN2−/− mice displayed differed microbiome profile from WT mice. …”
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Plant Growth Promotion and Biocontrol Potentiality of Endophytes Isolated from Root Nodules of Sulla flexuosa L. Plants by Samia Hamane, Anass El Yemlahi, Mounir Hassani Zerrouk, Ouiam El Galiou, Amin Laglaoui, Mohammed Bakkali, Abdelhay Arakrak

“…In vitro experiments revealed the strains’ potential to withstand salt and drought stresses by being able to grow in high concentrations of NaCl and PEG. Based on 16S rRNA gene sequencing, the strains were identified as Enterobacter and Serratia. …”
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Macrophage-derived extracellular vesicles as new players in chronic non-communicable diseases by Fengjuan Lin, Huiyu Luo, Jiexian Wang, Qing Li, Longying Zha

“…Extracellular vesicles (EVs) are released by cells and transport their contents (micro RNAs, mRNA, proteins, and long noncoding RNAs) to nearby or distant cells for cell-to-cell communication. …”
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Knockdown of zebrafish tmem242 enhances the production of ROS that signals to increase f9a expression resulting in DIC-like condition by Afnan Deebani, Jabila Mary, Sanchi Dhinoja, Ayah Al Qaryoute, Weam Fallatah, Pudur Jagadeeswaran

“…While thrombocyte production and aggregation were unaffected, coagulation was impaired, with delayed fibrin and thrombus formation. Notably, mRNA levels of several clotting factor genes, including coagulation factor 5 (f5), coagulation factor 7 (f7), and coagulation factor 9a (f9a), were elevated, except for coagulation factor 8 (f8). …”
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The Alteration and Clinical Significance of Th22/Th17/Th1 Cells in Patients with Chronic Myeloid Leukemia by Ping Chen, Min Wang, Daqi Li, Yan Jia, Na He, Wei Li, Daoxin Ma, Chunyan Ji

“…Plasma IL-22, IL-17, and IFN-r concentrations were measured by ELISA. AHR and RORC mRNA expressions were examined by RT-PCR. The frequencies of Th22, Th17, and Th1 cells, along with the expression of specific transcription factors RORC and AHR, were significantly decreased in ND patients compared with healthy controls, while all these abnormality recovered in CP patients. …”
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Blocking the Hepatic Branch of the Vagus Aggravates Hepatic Ischemia-Reperfusion Injury via Inhibiting the Expression of IL-22 in the Liver by Heng Zhou, Juling Xu, Sanxiong Huang, Ying He, Xiaowei He, Lu Guo, Shi Yin, Sheng Lu

“…Further experiments confirmed that Hv significantly downregulated the expression of IL-22 protein and mRNA in the liver, blocking the activation of the STAT3 pathway. …”
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Association of H-Type Hypertension with miR-21, miR-29, and miR-199 in Kazahks of Xinjiang, China by Xin He, RuLin Ma, Yu Li, Haixia Wang, YiZhong Yan, YiDan Mao, ShengYu Liao, XueYing Sun, ShuXia Guo, Heng Guo

“…Then, we analyzed the effect of MTHFR 677C > T polymorphism on the association between the above miRNA and H-type hypertension. Method. In this study, the expression of miR-21, miR-29, and miR-199 was quantitatively measured in 120 serum samples and then stratified according to the C677T polymorphism to analyze the relationship between target miRNAs and HHcy. …”
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Recent updates of centromere proteins in hepatocellular carcinoma: a review by Zhongyuan Yang, Wenjiao Chen, Yunhui Liu, Yuxin Niu

“…For example, small ribonucleic acid (RNA) can silence CENP expression and prevent the occurrence and development of liver cancer. …”
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ＵＲＳＡ患者蜕膜调节性Ｔ细胞Ｔｏｌｌ受体表达变化的临床意义

“…【结果】ＵＲＳＡ患者蜕膜ＣＤ４＋ＣＤ２５ｈｉｇｈ Ｔ细胞中ＴＬＲ４、ＴＬＲ９的表达在ｍＲＮＡ、蛋白水平均下调，差异有统计学意义（Ｐ ＜ ０．０１），而ＴＬＲ２表达两组间差异无统计学意义（Ｐ＞ ０．０５）。…”
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