Characterization of Streptomyces Isolates Associated with Estuarine Fish Chanos chanos and Profiling of Their Antibacterial Metabolites-Crude-Extract by Muhammad A. Kurnianto, Harsi D. Kusumaningrum, Hanifah N. Lioe

“…Based on the morphological, physiological, and biochemical characterization, as well as 16S rRNA partial sequencing, all of the four isolates belonged to Streptomyces. …”
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Identification and validation of hub gene TNFAIP6 of fibroblast-like synoviocytes in rheumatoid arthritis by LEI Lei, LYU Yuxin, ZHANG Jing

“…RT-qPCR and Western blot assay demonstrated that TNFAIP6 was significantly highly expressed at mRNA and protein levels in MH7A cells stimulated with 10 ng/mL TNF-α when compared to the cells treated with PBS (P<0.05). …”
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Notch Signaling Pathway Promotes Th17 Cell Differentiation and Participates in Thyroid Autoimmune Injury in Experimental Autoimmune Thyroiditis Mice by Hao Liu, Yiwen Li, Yujiao Zhu, Lei Ma, Haibo Xue

“…Correlation analyses revealed that the Th17 cell percentages were positively correlated with the serum TgAb titers, Notch pathway-related mRNA expression levels, and IL-17A concentrations in EAT mice (all P<0.05). …”
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<i>Macrostomum lignano</i> as a model to study the genetics and genomics of parasitic flatworms by K. V. Ustyantsev, V. Yu. Vavilova, A. G. Blinov, E. V. Berezikov

“…M. lignano has a number of convenient biological and experimental properties, such as fast reproduction, easy and non-expensive laboratory culturing, optical body transparency, obligatory sexual reproduction, annotated genome and transcriptome assemblies, and the availability of modern molecular methods, including transgenesis, gene knockdown by RNA interference, and in situ hybridization. All this makes M. lignano amenable to the most modern approaches of forward and reverse genetics, such as transposon insertional mutagenesis and methods of targeted genome editing by the CRISPR/Cas9 system. …”
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Human reference microbiome profiles of different body habitats in healthy individuals by Sujin Oh, Kyoung Un Park, Kyoung Un Park

“…Blood, saliva, and stool samples were subjected to 16S rRNA sequencing. Reference intervals of alpha diversity indices were calculated. …”
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慢性丙型肝炎患者并糖尿病的危险因素分析

“…空腹胰岛素?血甘油三酯及血清丙型肝炎病毒(HCV)RNA等相关指标进行检测?分析,比较两组之间各指标的差异,并将CHC+DM组分为携带者?轻度?中度?…”
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Adaptation of Archaeal Communities to Summer Hypoxia in the Sediment of Bohai Sea by Xiaoxiao Guo, Yanying Li, Guisheng Song, Liang Zhao, Jing Wang

“…In this study, the diversity, composition, and distribution of archaeal community in sediment along an inshore–offshore transect across the oxygen‐depleted area in the Bohai Sea were investigated in June, July, and August of 2021 by employing high‐throughput sequencing of 16S rRNA gene. Results indicated that the archaeal communities were dominated by Thermoproteota (80.61%), Asgardarchaeota (8.70%), and Thermoplasmatota (5.27%). …”
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Knockdown of Adra2a Increases Secretion of Growth Factors and Wound Healing Ability in Diabetic Adipose-Derived Stem Cells by Xiangyuan Zhao, Yong Zhang, Xinzhen Zuo, Shubai Wang, Xiao Dong

“…Therefore, the purpose of this study was to investigate whether the elevated Adra2a is involved in the diminished wound-healing capabilities of T2D ASCs. RNA-seq was used to compare the transcriptomic profiles of T2D and normal ASCs. …”
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血管紧张素Ⅱ诱导心肌细胞肥大后缝隙连接蛋白Cx43表达的变化 by 杨军, 伍卫, 梁蔚文, 王景峰, 潘秋辉, 方昶, 黄至斌

“…[结果]血管紧张素Ⅱ处理后的心肌细胞表现细胞肥大且细胞活力增强,S期、G2-M期细胞百分比增加,细胞内G2-M（二倍体）DNA含量降低,Cx43蛋白表达低于对照组,Cx43mRNA表达水平也较对照组明显下调,Cx43蛋白表达下调与细胞周期分布的改变相关。[结论]血管紧张素Ⅱ诱导心肌细胞肥大后Cx43基因表达明显下调,导致Cx43蛋白表达亦出现下调,这一改变可能与心肌肥大过程中的细胞周期变化有关,提示血管紧张素Ⅱ可能通过调控Cx43基因的表达而参与缝隙连接重构过程,而Cx43表达的变化可能与心肌肥大的机制有关。…”
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Plexin C1 Marks Liver Cancer Cells with Epithelial Phenotype and Is Overexpressed in Hepatocellular Carcinoma by Gorkem Odabas, Metin Cetin, Serdar Turhal, Huseyin Baloglu, A. Emre Sayan, Tamer Yagci

“…Our observations in cancer databases that PLXNC1 is upregulated in HCC led us to investigate the expression profile of Plexin C1 mRNA and protein in HCC cell lines and tissues. Methods. …”
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Associations of miR-499 and miR-34b/c Polymorphisms with Susceptibility to Hepatocellular Carcinoma: An Evidence-Based Evaluation by Zhongxia Wang, Junhua Wu, Guang Zhang, Yin Cao, Chunping Jiang, Yitao Ding

“…Single nucleotide polymorphisms (SNPs) in microRNA genes may be associated with susceptibility to HCC. …”
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Activating Transcription Factor 5 Promotes Tumorigenic Capability in Cervical Cancer Through the Wnt/&beta;-Catenin Signaling Pathway by Shi F, Wei Y, Huang Y, Yao D

“…Knockdown ATF5 expression in cervical cancer cell lines was constructed using lentivirus-mediated shRNA to explore the role of ATF5 in cervical cancer through cell viability, transwell, and wound healing experiments. …”
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PPAR-γ抑制剂T0070907对人鼻咽癌细胞的生长抑制作用

“…【目的】 探讨PPAR-γ选择性抑制剂T0070907影响鼻咽癌(NPC)细胞体外生长的作用机制65377;【方法】 通过RT-PCR和Western blotting检测PPAR-γ受体在5株NPC细胞株(CNE165380;CNE265380;HONE165380;SUNE1和5-8F)中mRNA和蛋白水平的表达;MTT法检测T0070907对NPC细胞生长情况的影响;流式细胞术和Western blotting检测T0070907对NPC细胞的细胞周期和细胞周期相关蛋白表达的影响65377;【结果】 PPAR-γ在5株NPC细胞中均有表达;T0070907对NPC细胞株有明显生长抑制作用,并呈浓度65380;时间依赖性;T0070907作用于NPC细胞CNE1和CNE2后,G2/M期细胞比例则逐渐增加,且随着处理浓度的增加而增加,各组间差异有统计学意义(P < 0.05)65377;此外,随着T0070907处理浓度的增加,NPC细胞的细胞周期相关蛋白Cyclin A65380;Cyclin B165380;Cdc265380;Cdk2蛋白的表达逐渐下降,无活性的pCdc2蛋白表达则逐渐增强65377;【结论】 PPAR-γ抑制剂T0070907能够抑制NPC细胞PPAR-γ的蛋白表达,并通过对细胞周期相关蛋白Cyclin A65380;Cyclin B165380;Cdc265380;Cdk265380;pCdc2等蛋白的调控导致细胞周期G2/M期阻滞,从而抑制NPC细胞的生长65377;…”
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Discovery of two novel Flavobacterium species with potential for complex polysaccharide degradation by Xu-Dong Lian, Yong Guan, Yue Jiang, Dong-Heui Kwak, Mi-Kyung Lee, Zhun Li

“…Through comprehensive analyses, including 16S rRNA gene sequencing, whole-genome sequencing, and detailed morphological, physiological, and chemotaxonomic characterizations, these strains have been identified as new species within the genus Flavobacterium. …”
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Intraspinal Grafting of Serotonergic Neurons Modifies Expression of Genes Important for Functional Recovery in Paraplegic Rats by Krzysztof Miazga, Hanna Fabczak, Ewa Joachimiak, Małgorzata Zawadzka, Łucja Krzemień-Ojak, Marek Bekisz, Anna Bejrowska, Larry M. Jordan, Urszula Sławińska

“…Here, we analyzed the mRNA of 5-HT2A and 5-HT7 receptors (encoded by Htr2a and Htr7 genes, resp.) in motoneurons innervating tibialis anterior (TA) and gastrocnemius lateralis (GM) hindlimb muscles and the tail extensor caudae medialis (ECM) muscle in intact as well as spinal rats. …”
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Gestational stress disrupts dopamine and oxytocin signaling in the postpartum reward system of rats: implications for mood, motivation and mothering by Achikam Haim, Christopher Albin-Brooks, Holly Brothers, Michaela Breach, Benedetta Leuner

“…A reduction in accumbal DA content as determined by liquid chromatography-mass spectrometry was also observed in gestationally-stressed dams. Lastly, we assessed mRNA expression of OT and OT receptors (OTR) and found that gestational stress increased OT expression in the hypothalamus but reduced OTR expression in the postpartum ventral tegmental area (VTA), a target of hypothalamic OT neurons. …”
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SPOCK2 controls the proliferation and function of immature pancreatic β-cells through MMP2 by Katarzyna Blaszczyk, Anna P. Jedrzejak, Natalia Ziojla, Ekaterina Shcheglova, Karolina Szarafin, Artur Jankowski, Christine A. Beamish, Jolanta Chmielowiec, Omaima M. Sabek, Ashok Balasubramanyam, Sanjeet Patel, Malgorzata Borowiak

“…Through bidirectional expression modulation and single-cell RNA-seq, we identified SPOCK2, an ECM protein, as an inhibitor of immature β-cell proliferation. …”
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Identified VCAM1 as prognostic gene in gastric cancer by co-expression network analysis by Wenjuan Li, Hong Gao, Jianjun Liu

“…This study aims to identify genetic variances and potential diagnostic and therapeutic approaches for diverse types of gastric cancer utilizing an extensive dataset. Data from RNA sequencing and clinical pathological details were acquired from The Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) dataset. …”
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BuDDI: Bulk Deconvolution with Domain Invariance to predict cell-type-specific perturbations from bulk. by Natalie R Davidson, Fan Zhang, Casey S Greene

“…BuDDI utilizes domain adaptation techniques to effectively integrate available corpora of case-control bulk and reference scRNA-seq observations to infer cell-type-specific perturbation effects. …”
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Hemorrhagic Shock Induces a Rapid Transcriptomic Shift of the Immune Balance in Leukocytes after Experimental Multiple Injury by Lisa Debler, Annette Palmer, Sonja Braumüller, Bettina Klohs, Tom Eirik Mollnes, Karlheinz Holzmann, Markus Huber-Lang, Rebecca Halbgebauer

“…Four hours after trauma, corresponding to three hours after induction of hemorrhage, RNA was isolated from all peripheral blood leukocytes, and a microarray analysis was performed. …”
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