Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7 by Changjiao Luan, Yue Gao, Jun Zhao, Xiaohui Zhang, Chaofan Wang, Wentao Sun, Yucheng Li, Xinxing Yang, Jiaxiao Chen, Weili Liu, Weijuan Gong, Xingjie Ma

“…In this study, we conducted RNA sequencing on bleomycin-induced senescent lung tissues from mice and identified Clic3 as the most significantly upregulated CLIC member. …”
Get full text

Pre-therapeutic efficacy of the CDK inhibitor dinaciclib in medulloblastoma cells by Marta Buzzetti, Sonia Morlando, Dimitrios Solomos, Ammara Mehmood, Alexander W. I. Cox, Mattia Chiesa, Yuri D’Alessandra, Michela Garofalo, Caroline H. Topham, Gianpiero Di Leva

“…We found that dinaciclib-triggered apoptosis is triggered by CDK9 inhibition and the resultant reduction in RNA pol II phosphorylation, which leads to the downregulation of the oncogenic marker MYC, and the anti-apoptotic protein MCL-1. …”
Get full text

Molecular Detection of Ehrlichia ruminantium in Cattle From Different Agro‐Ecological Zones of Cameroon: Implication for the Understanding of the Heartwater Epidemiology by Esthelline Yangea Tchounkeu, Barberine Assongo Silatsa, Rolin Mitterran Ndefo Kamga, Pythagore Soubgwi Fogue, Gustave Simo

“…DNA was extracted from blood and semi‐nested PCR targeting the 16S rRNA gene of E. ruminantium was used to search for this bacterium. …”
Get full text

Combined PARP14 inhibition and PD-1 blockade promotes cytotoxic T cell quiescence and modulates macrophage polarization in relapsed melanoma by Chun Wai Wong, Rotem Leshem, Kieran Neil Sefton, I-Hsuan Lin, Dervla Tamara Isaac, Mario Niepel, Adam Hurlstone

“…Using preclinical models and single-cell RNA sequencing, we investigated immune and tumor cell adaptations to PARP14 inhibition combined with PD-1 blockade.Results Combining PARP14 inhibition and PD-1 blockade suppressed tumor-associated macrophages while increasing proinflammatory memory macrophages. …”
Get full text

Exploring genetic structures and shared sites between alcohol, cheese intake, and inflammatory bowel disease by Zhifang Huang, Weichao Yuan

“…Furthermore, both conjFDR and MTAG analyses identified multiple shared genetic loci, with nine genes (Y_RNA, DENND1B, GCKR, KPNA7, CLN3, SLC39A8, FUT2, ERAP2, and SMAD3) being.ConclusionThe present study provides genetic evidence supporting the comorbidity of alcohol and cheese intake with IBD, offering novel insights into potential strategies for the prevention and treatment of IBD through the modulation of alcohol and cheese consumption.…”
Get full text

Characterization of infectious salmon anaemia virus survival and infectivity in seawater by Simon Chioma Weli, Marit Måsøy Amundsen, Ole Bendik Dale, Sonal Jayesh Patel

“…In addition, the intensity of positive IFAT staining of cells infected with the eluted spiked seawater was proportional to the viral load obtained by RT-qPCR. However, while viral RNA could be detected at high amounts in samples from day five and day eight respectively for ISAV-G and ISAV-H incubated at 15 ℃, we could not demonstrate infective particles from these samples. …”
Get full text

Microbiome analysis in individuals with human papillomavirus oral infection by David Israel Escobar Marcillo, Grete Francesca Privitera, Francesca Rollo, Alessandra Latini, Eugenia Giuliani, Maria Benevolo, Massimo Giuliani, Barbara Pichi, Raul Pellini, Maria Gabriella Donà

“…DNA purified from oral rinse-and-gargles of HIV-infected (HIV+) and HIV-uninfected (HIV−) individuals were used for 16S rRNA gene V3–V4 region amplification and sequencing. …”
Get full text

Impact of sex on the adaptation of adult mice to long consumption of sweet-fat diet by N. M. Bazhan, T. V. Iakovleva, A. D. Dubinina, E. N. Makarova

“…Weights of body, liver and fat depots, blood concentrations of hormones and metabolites, liver fat, and mRNA levels of genes involved in regulation of energy metabolism in the liver, perigonadal and subcutaneous white adipose tissue (pgWAT, scWAT) and brown adipose tissue (BAT) were measured. …”
Get full text

Polydatin ameliorates early brain injury after subarachnoid hemorrhage through up-regulating SIRT1 to suppress endoplasmic reticulum stress by Yuwei Han, Guangzhi Hao, Song Han, Tingzhun Zhu, Yushu Dong, Ligang Chen, Xinyu Yang, Xiaoming Li, Hai Jin, Guobiao Liang

“…Western blot and quantitative real-time polymerase chain reaction (PCR) analyses were performed to examine protein and mRNA expressions related to ER stress. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was used to detect cell apoptosis, and transmission electron microscopy was used to observe the ultrastructure of the endoplasmic reticulum.ResultsThe results indicated that PD significantly reduced brain edema and Evans blue extravasation after SAH, improving neurological function. …”
Get full text

Renal glomerular and tubular injury in the offspring of the preeclampsia-like syndrome by Yong Wang, Hao Wang, Huiqing Lu, Ji Ma, Wei Wu, Yinan Wang, Bo Ma, Hao Zhu, Rong Hu

“…At 1 week of age, the pre-eclampsia group exhibited elevated mRNA and protein expression levels of AT1R, GRK4, AQP2, ENaC, and NCC in renal tissues compared to controls. …”
Get full text

Comparative transcriptome analysis of albino northern snakehead (Channa argus) reveals its various collagen-related DEGs in caudal fin cells. by Shixi Chen, Ning Li, Fardous Mohammad Safiul Azam, Li Ao, Na Li, Jianlan Wang, Yuanchao Zou, Rui Li, Zakaria Hossain Prodhan

“…Additionally, there are gray- and golden-finned individuals, which exhibit distinct coloration in their caudal fins. We performed RNA-seq to profile the transcriptome of caudal fin tissues in albino gray-finned and golden-finned C. argus, contrasting these with normal morphs to elucidate the differences between the two groups. …”
Get full text

Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway by Hong Wei, Rui Bu, Qinghui Yang, Jing Jia, Tao Li, Qiuping Wang, Yanjun Chen

“…Exendin-4 treatment decreased the protein level of TXNIP. Moreover, RNA silencing of TXNIP mimicked the antipyroptotic actions of Exendin-4. …”
Get full text

不同营养组成食物对花鼠肠道菌群的影响 by 朱紫薇, 魏爽, Iram Maqsood, 司雨蕙, 钟涵, 戎可

“…为探究食物主要营养成分对花鼠（<italic>Tamias sibiricus</italic>）肠道菌群结构的影响，以人工饲料饲喂花鼠，以野外自由取食为对照，通过粪便采样，以16S rRNA高通量测序分析花鼠肠道菌群组成。依据食物来源和饲料中脂肪、淀粉含量的不同，将被饲喂的花鼠分为野外组、脂肪组、淀粉组和饲料组。…”
Get full text

Clinical characteristics of dengue virus infections in Karachi from 2019 to 2023: a cross-sectional study by Khalid Mahmood, Muhammad Rashid, Sabeeta Kanwal Ansari, Fatima Kanani, Thomas Iftner

“…This study aimed to bridge the gap by screening 1500 patients using immunochromatographic detection and clinically following up on 486 of them. RNA extraction and cDNA synthesis of positive patients were performed, followed by PCR and sequencing. …”
Get full text

The importance of fungi and of mycology for a global development of the bioeconomy by L. Lange, L. Bech, P.K. Busk, M.N. Grell, Y. Huang, M. Lange, T. Linde, B. Pilgaard, D. Roth, X. Tong

“…This knowledge can be obtained by studying the fungal proteome and metabolome; the biology of fungal RNA and epigenetics; protein expression, homologous as well as heterologous; fungal host/substrate relations; physiology, especially of extremophiles; and, not the least, the extent of global fungal biodiversity. …”
Get full text

Aqp 9 and Brain Tumour Stem Cells by Guri Fossdal, Einar O. Vik-Mo, Cecilie Sandberg, Mercy Varghese, Mari Kaarbø, Emily Telmo, Iver A. Langmoen, Wayne Murrell

“…However, immunostaining did not reveal increased protein expression of aqp9 in the tumourspheres containing progenitor cells; in contrast, its expression (both mRNA and protein) was high in differentiated cultures. …”
Get full text

Morphological, craniodental, and molecular characterization of Platyrrhinus guianensis (Chiroptera, Phyllostomidae) and second record for Brazil by Amanda C. da S. Lima, Samira B. Mendes, Ana P. M. Olímpio, Fábio H. S. Cardoso, Cleison L. da S. Costa, Elmary da C. Fraga, Maria C. Barros, Iracilda Sampaio

“…Molecular results provide the first public sequence of P. guianensis for the 16S rRNA gene and a new sequence for the COI gene, which will be useful in future studies for the species. …”
Get full text

Spatiotemporal Expression and Molecular Characterization of miR-344b and miR-344c in the Developing Mouse Brain by Jia-Wen Leong, Syahril Abdullah, King-Hwa Ling, Pike-See Cheah

“…MicroRNAs (miRNAs) are small noncoding RNA known to regulate brain development. The expression of two novel miRNAs, namely, miR-344b and miR-344c, was characterized during mouse brain developmental stages in this study. …”
Get full text

海南鳽贵州雷公山小种群遗传多样性分析 by 李斌强 詹昊峰 何小涛 吕小艳 杨宗才 王野影

“…为了解贵州省内海南鳽(<i>Gorsachius magnificus</i>)雷公山小种群的遗传多样性,通过分析海南鳽、黑冠鳽(<i>G.melanolophus</i>)和栗头鳽(<i>G.goisagi</i>)3种鸟类线粒体全基因组序列变异位点差异,筛选出<i>NADH</i>6<i>-tRNA^Glu</i>-D-loop序列作为海南鳽和夜鳽属物种鉴定的最优分子标记,对贵州省野生动物救护中心获得不明原因死亡的5只海南鳽进行扩增,并对所获序列进行碱基组成、遗传多样性、中性检验、错配分布和单倍型网络分析。…”
Get full text

Rhus Verniciflua Stokes Inhibits PD-1 Expression and Induces Anticancer Effects by Enhancing T Cell Function by Seoyoung Kim PhD, Young-Kwan Lee PhD, Wang-Jun Lee MD, PhD, Hyoun Jong Moon MD, PhD, Sanghun Lee KMD, PhD

“…The effect of RVS extracts on T cell phenotype was assessed using markers such as CTLA-4 and PD-1, as well as mRNA expression levels of key genes (IFN-γ, TNF-α, and IL-2). …”
Get full text