Sex and outcomes of patients with microsatellite instability-high and BRAF V600E mutated metastatic colorectal cancer receiving immune checkpoint inhibitors by Michael Hoffmeister, Hermann Brenner, Chiara Cremolini, Sara Lonardi, Filippo Pietrantonio, Julien Taieb, David Tougeron, Robyn L Ward, Marco Vitellaro, Thierry André, Pierre Laurent-Puig, Jitendra Jonnagaddala, Francesca Bergamo, Raghav Sundar, Claire Gallois, Michael J Overman, Jakob Nikolas Kather, Lisa Salvatore, Romain Cohen, Rossana Intini, Priya Jayachandran, Miriam Koopman, Javier Ros, Marwan Fakih, Vincenzo Nasca, Giacomo Mazzoli, Jeanine M L Roodhart, Filippo Ghelardi, Elena Elez, Durgesh Wankhede, Marta Ligero, Joseph Zhao, Koen Zwart, Jeroen Derksen, Nicholas Hawkins, Javier Carmona

“…Finally, we explored two public microarray and RNA-seq datasets from patients with non-metastatic or metastatic MSI-H CRC to gain translational insights on the association between sex, BRAF status and immune contextures/ICI efficacy.Results Although no differences were observed between females and males either overall or in the BRAF wild-type cohort, male sex was associated with inferior progression-free survival (PFS) and overall survival (OS) in the BRAF mutated cohort (in multivariable models, HR for PFS: 1.79, 95% CI: 1.13 to 2.83, p=0.014, and for OS: 2.33, 95% CI: 1.36 to 3.98, p=0.002). …”
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Integrated microbiome, transcriptome and metabolome insight into the alleviating mechanisms of sheep milk in a DSS-induced colitis mouse model by Wenna Yao, Ruilin Du, Shuo Yan, Teligun Bao, Huimin Zhang, Fang Yang, Yue Xue, Yulong Zhao, Siqin Bao, Xihe Li, Yongli Song

“…ELISA and qPCR analysis showed that relative mRNA expression levels of TNF-α, IL-1β, IL-6, and IL-17 were decreased in SM treatment compared to DSS-induced colitis mice. …”
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Stromal architecture and fibroblast subpopulations with opposing effects on outcomes in hepatocellular carcinoma by Yifei Cheng, Xiaofang Chen, Li Feng, Zhicheng Yang, Liyun Xiao, Bin Xiang, Xiaodong Wang, Dongbin Liu, Penghui Lin, Jieyi Shi, Guohe Song, Wulei Qian, Boan Zhang, Yanan Xu, Zheng Gao, Lv Chen, Yingcheng Wu, Jiaqiang Ma, Youpei Lin, Haichao Zhao, Lihua Peng, Xuebin Mao, Yang Liu, Hao Hou, Mingyu Yang, Yuan Ji, Xiaoying Wang, Jian Zhou, Xun Xu, Xiyang Liu, Wu Wei, Xiaoming Zhang, Qiang Gao, Hu Zhou, Yidi Sun, Kui Wu, Jia Fan

“…Using paired single-cell RNA and epigenomic sequencing with Stereo-seq, we revealed two fibroblast subsets CAF-FAP and CAF-C7, whose spatial enrichment strongly correlated with the two stromal archetypes and opposing patient prognosis. …”
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Effects of rumen fluid transplantation on the fecal flora of yaks by Man Zhang, Man Zhang, Man Zhang, Yan Li, Yumin Li, Yumin Li, Yumin Li, Chenxu Cheng, Chenxu Cheng, Chenxu Cheng, Shatuo Chai, Shatuo Chai, Shatuo Chai, Yingkui Yang, Yingkui Yang, Yingkui Yang, Xun Wang, Xun Wang, Xun Wang, Shuxiang Wang, Shuxiang Wang, Shuxiang Wang

“…After this, yak feed intake was recorded daily; yaks were weighed on days 1, 30, and 60 of the formal trial; and yak feces were collected directly from the ground on days 1, 4, 7, 14, 30, and 60 to compare the microbial composition of the feces using 16S rRNA sequencing data.ResultsThe results showed that rumen fluid transplantation significantly increased the alpha diversity of fecal microflora (P < 0.05), and on day 30 of the experiment, both the operational taxonomic unit (OTU) and Shannon index were significantly higher in the RT group than the CON group (P < 0.05). …”
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Dicoumarol sensitizes hepatocellular carcinoma cells to ferroptosis induced by imidazole ketone erastin by Ziwei Yang, Ziwei Yang, Tixin Han, Ruibin Yang, Ruibin Yang, Yinuo Zhang, Yinuo Zhang, Yifei Qin, Yifei Qin, Yifei Qin, Jialu Hou, Jialu Hou, Fei Huo, Fei Huo, Zhuan Feng, Zhuan Feng, Yaxin Ding, Yaxin Ding, Jiali Yang, Jiali Yang, Gang Zhou, Gang Zhou, Shijie Wang, Shijie Wang, Xiaohang Xie, Xiaohang Xie, Peng Lin, Peng Lin, Zhi-Nan Chen, Zhi-Nan Chen, Jiao Wu, Jiao Wu

“…The intrinsic factors determining sensitivity to IKE-induced ferroptosis remain to be explored to improve its efficacy.MethodsBulk RNA-sequencing data from hepatocellular carcinoma (HCC) and normal liver tissues were collected from The Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression (GTEx) databases. …”
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Reprogramming of iPSCs to NPCEC-like cells by biomimetic scaffolds for zonular fiber reconstruction by Tianhui Chen, Zhongxing Chen, Juan Du, Min Zhang, Zexu Chen, Qingyi Gao, Aodong Chen, Qinghao Meng, Yang Sun, Yan liu, Linghao Song, Xinyue Wang, Pranav Prakash Edavi, Chen Xu, Hongmei Zhang, Jinhai Huang, Yongxiang Jiang

“…Ocular tissue and aqueous humour samples from patients with EL were subjected to RNA sequencing and Olink high-throughput proteomic analysis, revealing the downregulation of pathogenic genes (FBN1, MFAP2) and upregulation of secretory proteins (IL-12, MMP-1). …”
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Impaired inflammatory resolution with severe SARS-CoV-2 infection in leptin knock out obese hamster by Ren-Di Jiang, Yun-Zhe Luo, Hao-Feng Lin, Xiao-Shuang Zheng, Wen-Tao Zeng, Mei-Qin Liu, Hao-Hao Deng, Qi Wang, Ya-Na Lai, Ying Chen, Zi-Shuo Guo, Ya Zeng, Qian-Chun Gong, Chen Qiu, Mei Dong, Xi Wang, Zi-Yi Wang, Li-Na Ji, Pan-Pan Hou, Qian Li, Xu-Rui Shen, Bei Li, Yun Gao, Ai-Hua Zhang, Ting-Ting Jiang, Ai-Min Shi, Peng Zhou, Xin-Hua Lin, Zi-Qing Deng, Jian-Min Li, Zheng-Li Shi

“…Notably, reduced Trem2-dependent efferocytosis pathways in Leptin−/− hamsters indicated weakened inflammation resolution, consistent with the scRNA-seq data from patients with obesity. In summary, our study highlights the obesity-associated mechanisms underlying severe SARS-CoV-2 infections and establishes a reliable preclinical animal model for developing obesity-specific therapeutics for critical COVID-19.…”
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AdipoRon ameliorates the progression of heart failure with preserved ejection fraction via mitigating lipid accumulation and fibrosis by Wuping Tan, Yijun Wang, Siyi Cheng, Zhihao Liu, Mengjie Xie, Lingpeng Song, Qinfang Qiu, Xiaofei Wang, Zeyan Li, Tianyuan Liu, Fuding Guo, Jun Wang, Xiaoya Zhou

“…Cardiac function was evaluated using echocardiography, and Postmortem analysis included RNA-sequencing, untargeted metabolomics, transmission electron microscopy and molecular biology methods. …”
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Delivery of dendritic cells targeting 3M2e-HA2 nanoparticles with a CpG adjuvant via lysosomal escape of Salmonella enhances protection against H9N2 avian influenza virus by Futing Jia, Wenfeng Wang, Yawen Tian, Ainul Zahra, Yingkai He, Chongbo Ge, Tongyu Zhang, Mingyue Wang, Jingshuo Gong, Gerui Zhang, Guilian Yang, Wentao Yang, Chunwei Shi, Jianzhong Wang, Haibin Huang, Xin Cao, Yang Zeng, Nan Wang, Zhannan Wang, Chunfeng Wang, Yanlong Jiang

“…All the constructed plasmids were then transformed into the sifA gene-deficient Salmonella vector χYL56 for oral immunization.The results showed that sifA-deficient Salmonella could efficiently increase antigen-specific mucosal sIgA antibody titers, especially in alveolar lavage samples, whereas the presence of the phage-54 nanobody could dramatically increase intracellular IFN-γ mRNA levels, indicating its ability to enhance the Th1-type immune response. …”
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Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti... by Arndt Borkhardt, Nan Qin, Stephen T Keir, Darell D Bigner, Allison Cole, Matthias Wölfl, Viktoria Marquardt, Johanna Theruvath, David Pauck, Daniel Picard, Lena Blümel, Mara Maue, Jasmin Bartl, Ulvi Ahmadov, Maike Langini, Frauke-Dorothee Meyer, Joselyn Cruz-Cruz, Claus M Graef, Till Milde, Olaf Witt, Anat Erdreich-Epstein, Gabriel Leprivier, Ulf Kahlert, Anja Stefanski, Kai Stühler, Julia Hauer, Thomas Beez, Christiane B Knobbe-Thomsen, Ute Fischer, Jörg Felsberg, Finn K Hansen, Rajeev Vibhakar, Sujatha Venkatraman, Samuel H Cheshier, Guido Reifenberger, Thomas Kurz, Marc Remke, Siddhartha Mitra

“…We tested the combinatorial effect of targeting class I HDACs and the CD47-SIRPa phagocytosis checkpoint pathway using in vitro phagocytosis assays and in vivo orthotopic xenograft models.Results CI-994 displayed antitumoral effects at the primary site and the metastatic compartment in two orthotopic mouse models of MYC-driven MB. Furthermore, RNA sequencing revealed nuclear factor-kB (NF-κB) pathway induction as a response to CI-994 treatment, followed by transglutaminase 2 (TGM2) expression, which enhanced inflammatory cytokine secretion. …”
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A 5-transcript signature for discriminating viral and bacterial etiology in pediatric pneumonia by Sandra Viz-Lasheras, Alberto Gómez-Carballa, Jacobo Pardo-Seco, Xabier Bello, Irene Rivero-Calle, Ana Isabel Dacosta, Myrsini Kaforou, Dominic Habgood-Coote, Aubrey J. Cunnington, Marieke Emonts, Jethro A. Herberg, Victoria J. Wright, Enitan D. Carrol, Stephane C. Paulus, Werner Zenz, Daniela S. Kohlfürst, Michiel Van der Flier, Ronald de Groot, Luregn J. Schlapbach, Philipp Agyeman, Andrew J. Pollard, Colin Fink, Taco T. Kuijpers, Suzanne Anderson, Cristina Calvo, María del Carmen Martínez-Padilla, Ana Pérez-Aragón, Esteban Gómez-Sánchez, Juan Valencia-Ramos, Francisco Giménez-Sánchez, Paula Alonso-Quintela, Laura Moreno-Galarraga, Ulrich von Both, Marko Pokorn, Dace Zavadska, María Tsolia, Clementien L. Vermont, Henriëtte A. Moll, Michael Levin, Federico Martinón-Torres, Antonio Salas

“…Our research seeks to address this gap by identifying host transcriptomic biomarkers in the blood of children with definitive viral and bacterial pneumonia. We performed RNA sequencing on 192 prospectively collected whole blood samples, including 38 controls and 154 pneumonia cases, uncovering a 5-transcript signature (genes FAM20A, BAG3, TDRD9, MXRA7, and KLF14) that effectively distinguishes bacterial from viral pneumonia (area under the curve (AUC): 0.95 [0.88–1.00]). …”
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Advanced delivery systems for gene editing: A comprehensive review from the GenE-HumDi COST Action Working Group by Alessia Cavazza, Francisco J. Molina-Estévez, Álvaro Plaza Reyes, Victor Ronco, Asma Naseem, Špela Malenšek, Peter Pečan, Annalisa Santini, Paula Heredia, Araceli Aguilar-González, Houria Boulaiz, Qianqian Ni, Marina Cortijo-Gutierrez, Kristina Pavlovic, Inmaculada Herrera, Berta de la Cerda, Emilio M. Garcia-Tenorio, Eva Richard, Sergio Granados-Principal, Arístides López-Márquez, Mariana Köber, Marijana Stojanovic, Melita Vidaković, Irene Santos-Garcia, Lorea Blázquez, Emily Haughton, Dongnan Yan, Rosario María Sánchez-Martín, Loubna Mazini, Gloria Gonzalez Aseguinolaza, Annarita Miccio, Paula Rio, Lourdes R. Desviat, Manuel A.F.V. Gonçalves, Ling Peng, Cecilia Jiménez-Mallebrera, Francisco Martin Molina, Dhanu Gupta, Duško Lainšček, Yonglun Luo, Karim Benabdellah

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Identification and subcellular localization of proteins that interact with Duck plague virus pUL14 in infected host cells by Jieyu Wan, Mingshu Wang, Anchun Cheng, Wei Zhang, Qiao Yang, Bin Tian, Xumin Ou, Di Sun, Yu He, Xinxin Zhao, Ying Wu, Shaqiu Zhang, Juan Huang, Zhen Wu, YanLing Yu, Ling Zhang, Dekang Zhu, Renyong Jia, Mafeng Liu, Shun Chen

“…These proteins are associated mainly with the regulation of biological processes, RNA biosynthetic processes, and nuclear export. …”
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