Vaginal microbiome distinction in women with HPV+, cervical intraepithelial neoplasia, and cervical cancer, a retrospective study by Yuanyue Li, Yuanyue Li, Xiaomei Wu, Xiaomei Wu

“…This study aimed to investigate the correlation between the vaginal microbiota, HPV infection, cervical intraepithelial neoplasias (CINs), and cervical cancers in 69 women.MethodsDNA was extracted from vaginal samples, followed by HPV genotyping through PCR and sequenced of the16S rRNA gene.ResultsOur results revealed that Lactobacillus was the predominant bacterium across all groups, with prevalence rates of 60.2% in women with HPV+, 63.9% in CINI, 97.7% in CINII, 52.0% in CINIII, 36.9% in cervical cancer, and 70.9% in NILM (normal cytology). …”
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Gene Expression Analysis of Deduced Protein Sequence from the FLOWERING LOCUS C (FLC) Homolog during Vegetative and Reproductive Phases in Lepidium sativum L. by Parissa Jonoubi, Farkhondeh Rezanejad, Fatemeh Mahyapoor

“…Results showed that LsFLC expression levels were very high in the root and relatively high in leaves and stems, whereas no expression was allowed in flowers. Indeed, the mRNA levels were much higher during the vegetative phase compared to the transition to flowering, therefore agreeing with the known role of FLC as a promoter of vegetative growth and an inhibitor of flowering. …”
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Blockade of Vascular Endothelial Growth Factor Receptor 1 Prevents Inflammation and Vascular Leakage in Diabetic Retinopathy by Jianbo He, Hong Wang, Ying Liu, Wen Li, Dorothy Kim, Hu Huang

“…Protein expression and localization were examined by western blots (WB) and immunofluorescence (IF). mRNA expression was quantified by PCR array and real-time PCR. …”
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Possible Roles of Proinflammatory and Chemoattractive Cytokines Produced by Human Fetal Membrane Cells in the Pathology of Adverse Pregnancy Outcomes Associated with Influenza Viru... by Noboru Uchide, Kunio Ohyama, Toshio Bessho, Makoto Takeichi, Hiroo Toyoda

“…Influenza virus infection induced the mRNA expression of not only the proinflammatory cytokines but also chemoattractive cytokines, such as monocyte chemoattractant protein (MCP)-1, regulated on activation, normal T-cell expressed and secreted (RANTES), macrophage inflammatory protein (MIP)-1β, IL-8, growth-regulated oncogene (GRO)-α, GRO-β, epithelial cell-derived neutrophil-activating protein (ENA)-78, and interferon inducible protein (IP)-10 in cultured chorion cells. …”
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Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain by Nourhan A. Zaghloul, Mona K. Gouda, Yasser Elbahloul, Nancy M. El Halfawy

“…Among twenty Pseudomonas aeruginosa isolates, we identified one isolate that demonstrated potent and remarkable azurin synthesis using the VITEK 2 system and 16S rRNA sequencing. The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active. …”
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Identification of KIFC1 as an independent prognostic marker in renal clear cell carcinoma correlates with tumor proliferation and immune infiltration by Bin Du, Jia Wang, Jinping Zheng, Jing Huo, Pu Wang

“…We analyzed the prognostic value, associated signaling pathways, and regulatory mechanisms of KIFC1 in ccRCC through bioinformatics and proteomics. Concretely, both mRNA and protein expression levels of KIFC1 were dramatically upregulated. …”
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Diversity of microbial communities in cigar filler leaves with different initial water contents analyzed based on high-throughput sequencing technology by Yumeng Gao, Yumeng Gao, Yihui Wang, Bingqing Hou, Guo Zhang, Chun Jiang, Song Fang, Qian Wang, Yang Ning

“…IntroductionTo study the composition and succession of bacterial and fungal communities during the fermentation of cigar filler leaves with varying initial water contents, high-throughput sequencing technology was used to sequence the bacterial 16SrRNA genes and fungal ITS1 genes from cigar tobacco leaf samples. …”
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Evaluation of One Step Nucleic Acid Amplification for detection of lymph node metastases compared to histopathological ultrastaging in women with endometrial cancer: a protocol for... by Jan Kostun, Alessandra Mescalchin, Martin Pešta, Robert Slunečko, Khaled M. Ismail, Vendula Smoligová, Jiri Presl

“…Participating units will be trained on the correct usage of the OSNA RD-210 analyser and nucleic acid amplification reagent kit LYNOAMP CK19 E for rapid detection of metastatic nodal involvement, based on the cytokeratin 19 (CK19) mRNA detection. Endometrial cancer patients ≥ 18 years listed for surgical treatment with sentinel lymph node mapping, with no history of other types of cancer and who provide a valid written consent will be considered potentially eligible for the study. …”
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Sevuparin strongly reduces hepcidin expression in cells, mice, and healthy human volunteers by Michela Asperti, Andrea Denardo, Magdalena Gryzik, Kristina E. M. Persson, Göran Westerberg, John Öhd, Maura Poli

“…These effects were evident in C57BL/6J mice after intravenous injection of a single dose of sevuparin (20 mg/kg) with a 70% reduction of hepcidin mRNA. Remarkably, similar effects were observed in healthy volunteers following single subcutaneous doses at 3, 6, and 9 mg/kg with 40%–50% suppression at 3 and 6 mg/kg and 72% at 9 mg/kg. …”
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MiR-134 在MA 诱导神经元损伤中的表达及其对神经诱发动作电位的影响 by 李涛, 王洪杰, 刘桂阳, 张博, 赵文博, 刘天蔚, 朱凯文, 孙晋浩

“…摘要：【目的】探讨微小非编码RNA分子miR-134在甲基苯丙胺（MA）诱导PC12神经细胞损伤中的表达变化及其对神经兴奋性的影响，为理解MA导致神经损伤的机制提供实验基础。…”
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Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences by Jianfeng Sun, Martin Philpott, Danson Loi, Gabriela Hoffman, Jonathan Robson, Neelam Mehta, Eleanor Calcutt, Vicki Gamble, Tom Brown, Tom Brown, Udo Oppermann, Adam P. Cribbs

“…Abstract Single-cell transcriptomics, which utilises barcodes and unique molecular identifiers (UMIs) for polyA+ mRNA capture, is compromised by oligonucleotide synthesis errors. …”
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PSTK exerts protective role in cisplatin‐tubular cell injury via BAX/BCL2/Caspase3 pathway by Yifan Wu, Yuanyuan Xv, Limei Zhao, Ziqi Zhou, Miaomiao Wang, Jima Xi, Ying Liming, Jianling Gao, Bingqing Deng, Dong Zheng

“…Abstract Cisplatin is a widely used anticancer drug, but its accumulation in renal tubular epithelial cells (TECs) can cause acute kidney injury. Phosphoseryl‐tRNA kinase (PSTK) is an intermediate product produced under oxidative stress conditions. …”
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Sex differences in the expression of lipid oxidation and glucose uptake genes in muscles of fasted mice by N. A. Feofanova, T. V. Yakovleva, E. N. Makarova, N. M. Bazhan

“…None of the genes studied (Ucp3, Cpt1 and Slc2a4) showed sex-related changes at mRNA levels in control groups, but females exposed to fasting demonstrated a significant increase in the expression of all genes as compared to control. …”
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Melanophilin-induced primary cilia promote pancreatic cancer metastasis by Yu-Ying Chao, Ruei-Ci Lin, Ping-Jui Su, Chu-An Wang, Ting-Yuan Tu, Ya-Chin Hou, Yi-Tzui Tsai, I-Chen Peng, Shaw-Jenq Tsai, Yan-Shen Shan, Chia-Yih Wang

“…Here, we demonstrated that melanophilin (MLPH) promotes PDAC metastasis by inducing the regrowth of primary cilia. Using RNA sequencing, we found that MLPH was upregulated in Gln-deficient conditions. …”
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The proline-rich antimicrobial peptide Api137 disrupts large ribosomal subunit assembly and induces misfolding by Simon Malte Lauer, Jakob Gasse, Andor Krizsan, Maren Reepmeyer, Thiemo Sprink, Rainer Nikolay, Christian M. T. Spahn, Ralf Hoffmann

“…High-resolution structures obtained by cryogenic electron microscopy demonstrate that a large proportion of pre-50S states are missing up to five proteins (uL22, bL32, uL29, bL23, and uL16) and have misfolded helices in 23S rRNA domain IV. These data suggest a second mechanism for Api137, wherein it disrupts 50S subunit assembly by inducing the formation of misfolded precursor particles potentially incapable of evolving into active ribosomes, suggesting a bactericidal mechanism.…”
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The Combination of Host-Associated Bacillus megaterium R32 and Stachyose Promotes the Intestinal Health of Turbot (Scophthalmus maximus. L) by Yaoyao Kong, Sifan Zhao, Weihao Ou, Kangsen Mai, Yanjiao Zhang

“…The results showed that diets RS and RC significantly inhibited the expression of cell development and apoptosis-related genes (β-catenin, CyclinD1, BAX, Bid); diets RS and RC significantly increased the expression of intestinal tight junction protein claudin-4, while RS group significantly decreased the expression of myosin light chain kinase; diets RS and RC significantly decreased the expression of proinflammatory factors (IL-13, IL-15, IFN- γ), diet RS also significantly decreased the expression of TNF-α and AP-1, and increased the expression of TGF-β. 16s rRNA gene sequencing results showed that diets RS and RC significantly decreased the abundance of conditional pathogenic bacteria (Corynebacterium, Desulfovibrio, Escherichia-Shigella). …”
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Arabidopsis apoplast TET8 positively correlates to leaf senescence, and tet3tet8 double mutants are delayed in leaf senescence by Jayde A. Zimmerman, Benjamin Verboonen, Andrew P. Harrison Hanson, Luis R. Arballo, Judy A. Brusslan

“…The increase in apoplast TET8 in the pen1 mutant did not correspond to increased TET8 mRNA levels. In addition, apoplast TET8 was more abundant in the early leaf senescence myb59 mutant, meaning the increase in apoplast TET8 protein during leaf senescence is not dependent on pen1 or pen3. …”
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Dysregulation of Podocyte BK Channels and Nephrosis: Effects of Circulating Factors and Auxiliary β4 Subunits by Eun Young Kim, Patrycja Rachubik, Stuart E. Dryer

“…The effect of TNFα on the surface expression of KCa1.1 was eliminated following siRNA knockdown of the β4 subunits, suggesting a role for this subunit in KCa1.1 trafficking to the cell surface. …”
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Rutin attenuates zearalenone-induced ferroptosis of endometrial stromal cells in piglets through the p53 signaling pathway by Chenlong Wang, Chuangjiang Chen, MengYa Wang, Sajid Ur Rahman, Bin Wei, Hongyan Ding, Wanyue Huang, Xichun Wang

“…When porcine ESCs were co-cultured with the addition of Rutin and ZEA following p53 gene silencing via siRNA transfection, Rutin significantly mitigated ZEA-induced mitochondrial damage, oxidative stress, and Fe2 + content through the p53 pathway. …”
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Canagliflozin attenuates kidney injury, gut-derived toxins, and gut microbiota imbalance in high-salt diet-fed Dahl salt-sensitive rats by Lili He, Qingjuan Zuo, Sai Ma, Guorui Zhang, Zhongli Wang, Tingting Zhang, Jianlong Zhai, Yifang Guo

“…This study employed 16S rRNA sequencing technology and liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based metabolic profiling combined with advanced differential and association analyses to investigate the correlation between the microbiome and the metabolome in male DSS rats.Results A high-salt diet disrupted the balance of the intestinal flora and increased toxic metabolites (methyhistidines, creatinine, homocitrulline, and indoxyl sulfate), resulting in severe kidney damage. …”
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