Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms by Mie Suzuki Okutani, Shinya Okamura, Tang Gis, Hitomi Sasaki, Suni Lee, Akiho Kashiwabara, Simon Goto, Mai Matsumoto, Mayuko Yamawaki, Toshiaki Miyazaki, Tatsuya Nakagawa, Masahito Ikawa, Wataru Kamitani, Shiro Takekawa, Koichi Yamanishi, Hirotaka Ebina

“…mRNA vaccines against SARS-CoV-2 were rapidly developed and were effective during the pandemic. …”
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Association of Corticosteroid Inhaler Type with Saliva Microbiome in Moderate-to-Severe Pediatric Asthma by Amir Hossein Alizadeh Bahmani, Mahmoud I. Abdel-Aziz, Simone Hashimoto, Corinna Bang, Susanne Brandstetter, Paula Corcuera-Elosegui, Andre Franke, Mario Gorenjak, Susanne Harner, Parastoo Kheiroddin, Leyre López-Fernández, Anne H. Neerincx, Maria Pino-Yanes, Uroš Potočnik, Olaia Sardón-Prado, Antoaneta A. Toncheva, Christine Wolff, Michael Kabesch, Aletta D. Kraneveld, Susanne J. H. Vijverberg, Anke H. Maitland-van der Zee, on behalf of the SysPharmPediA consortium

“…<b>Methods</b>: Saliva samples collected from 143 children (6–17 yrs) with moderate-to-severe asthma across four European countries (The Netherlands, Germany, Spain, and Slovenia) as part of the SysPharmPediA cohort were subjected to 16S rRNA sequencing. The microbiome was compared using global diversity (α and β) between two groups of participants based on inhaler devices (MDI (n = 77) and DPI (n = 65)), and differential abundance was compared using the Analysis of Compositions of Microbiomes with the Bias Correction (ANCOM-BC) method. …”
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Clinical and molecular outcomes from the 5-Year natural history study of SSADH Deficiency, a model metabolic neurodevelopmental disorder by Itay Tokatly Latzer, Jean-Baptiste Roullet, Wardiya Afshar-Saber, Henry H. C. Lee, Mariarita Bertoldi, Gabrielle E. McGinty, Melissa L. DiBacco, Erland Arning, Melissa Tsuboyama, Alexander Rotenberg, Thomas Opladen, Kathrin Jeltsch, Àngels García-Cazorla, Natalia Juliá-Palacios, K. Michael Gibson, Mustafa Sahin, Phillip L. Pearl

“…GABA-metabolism in induced GABAergic neurons could be reversed using CRISPR correction of the pathogenic variants or mRNA transfection and SSADHD iPSCs were associated with excessive glutamatergic activity and related synaptic excitation. …”
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Clinicopathological and molecular characterization of astrocytoma by Xiaoyan Wu, Wenfeng Peng, Xu Zhang, Tao Tang, Ling Deng, Yuxia Xu, Xiaoyun Liu, Fang Wang, Wujian Peng, Jianrong Huang, Xiaoni Zhong

“…Fluorescence in situ hybridization (FISH) using break-apart probes showed EWRS1 breaks in three patients and MN1 breaks in one. Further DNA or RNA-targeted biallelic sequencing identified an EWSR1(Exon1-7)-BEND2(Exon2-14) fusion in case 1, and an EWSR1(Exon1-7)-BEND2(Intergenic) fusion in case 2. …”
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Exploring the role of ESR1 mutations in metastatic hormone receptor-positive breast cancer T cell immune surveillance disruption by Morgane Lopez, Laurie Spehner, Fabrice André, Julien Viot, Evan Seffar, Amélie Marguier, Elsa Curtit, Guillaume Meynard, Erion Dobi, Sylvain Ladoire, Romain Boidot, Romain Loyon, Valentin Derangere, François-Clément Bidard, Christophe Borg, Laura Mansi, Marie Kroemer

“…A vaccination assay on a humanized mouse model (HLA-A2/DR1) was used to validate the immunogenicity and the presentation of these peptides. Finally, we used Bulk RNA-Seq sequencing along with MCPcounter, a cellular deconvolution method, to investigate the immune contexture of ESR1-mutated BC. …”
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GDF15 induces immunosuppression via CD48 on regulatory T cells in hepatocellular carcinoma by Meng Li, Yuan Gao, Wei Zhang, Zhijun Tan, Kuo Zhang, Zhaowei Wang, Lei He, Weina Li, Chuanyang Xu, Jieyu Zhang, Desheng Wang, Kefeng Dou, Ran Zhuang, Boquan Jin, Qiang Hao, Wangqian Zhang, Shuning Wang, Jintao Gu, Lei Shang, Haichuan Su, Yingqi Zhang, Cun Zhang

“…We used flow cytometry, coimmunoprecipitation, RNA sequencing, mass spectrum, chromatin immunoprecipitation and Gdf15–/–, OT-I and GFP transgenic mice to demonstrate the effects of GDF15 on Treg cells and related molecular mechanism. …”
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آنالیز گسترده ژنوم، تفسیر و بررسی بیان ژن‌های VDAC در گل رز چینی by گل محمد اشرفی, مصطفی خوشحال سرمست, جین لیو

“…پیش‌بینی ساختار سوم پروتئین با استفاده از نرم افزار Pymol و آنالیز RNA seq با استفاده از Mev انجام شد. یافته‌هانتایج جداسازی و توالی‌یابی ناحیه کد کننده دو ژن VDAC1 و VDAC2 نشان از شباهت 100 درصدی ژن‌های جدا شده و تطابق آن با پایگاه داده گل رز چینی دارد. …”
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Enhancing reproductive capacity in hybrid Red Tilapia (Oreochromis niloticus ×‎ O. mossambicus) via dietary administration of date palm pollen (Phoenix dactylifera L.)‎ by Nashi K. Alqahtani, Hesham S. Ghazzawy, Roshmon Thomas Mathew, Yousef‎ Ahmed Alkhamis, Rashid Saleh Alngada, Moaheda E.H. Eissa, Sameh A. Abdelnour, Sara F. Ghanem, Layla A. Almutairi, El-Sayed Hemdan Eissa

“…The GSI (%) and HIS (%), mean number of fry/fish, egg diameter (mm), and their weights were improved in all DPP fish groups compared to the DPP0 group. The mRNA expression levels of reproductive-related genes, including FSHR, CYP1A, ESR1, FOXL2A, and ESR2A were significantly upregulated in the gonads of fish fed DPP diets compared to the control group (P < 0.05). …”
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RECQL4 affects MHC class II‐mediated signalling and favours an immune‐evasive signature that limits response to immune checkpoint inhibitor therapy in patients with malignant melan... by Sara Egea‐Rodriguez, Renáta Váraljai, Thierry M. Nordmann, Restuan Lubis, Manuel Philip, Florian Rambow, Alexander Roesch, Michael Flaig, Susanne Horn, Raphael Stoll, Fang Zhao, Annette Paschen, Bert Klebl, Ian D. Hickson, Dirk Schadendorf, Matthias Mann, Iris Helfrich

“…Methods We analysed whole exome and bulk RNA sequencing data from a pan‐cancer cohort of 25 775 patients and cutaneous melanoma cohorts (untreated: n = 471, anti‐progressive disease [PD]‐1 treated: n = 212). …”
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Bone marrow mesenchymal stem cells derived cytokines associated with AKT/IAPs signaling ameliorate Alzheimer’s disease development by Yalan Lu, Yanfeng Xu, Li Zhou, Siyuan Wang, Yunlin Han, Kewei Wang, Chuan Qin

“…The behavioral tests, including open-field test, novel object recognition test, and Morris water maze were performed, followed by β-amyloidosis plaque and neuron apoptosis analyses. Then the tissue RNA sequencing and mBMMSC cytokine analysis were performed. …”
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An anoikis-related signature predicts prognosis and immunotherapy response in gastrointestinal cancers by Ruyi Liu, Ruyi Liu, Ruyi Liu, Ruyi Liu, Ruyi Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Yuchen Liu, Weicheng Huang, Weicheng Huang, Weicheng Huang, Weicheng Huang, Weicheng Huang, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Pengxiang Chen, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng, Yufeng Cheng

“…We aimed to explore the roles of anoikis-related genes (ARGs) in the GI cancer prognosis.MethodsWe extracted RNA sequencing and clinical data from The Cancer Genome Atlas and Gene Expression Omnibu databases for patients with esophageal cancer, gastric cancer, colon cancer and rectal cancer and identified ARGs from GeneCards and Harmonizome. …”
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Transient lymphocyte count decrease correlates with oncolytic adenovirus efficacy in humans: mechanistic and biomarker findings from TUNIMO phase I trial by Tuomo Alanko, Akseli Hemminki, Víctor Cervera-Carrascón, Riikka Havunen, Kristian Taipale, Dafne C A Quixabeira, Jorma Sormunen, Katriina Peltola, Suvi Sorsa, Santeri A Pakola, Elise Jirovec, Lyna Haybout, Claudia Kistler, Tatiana V Kudling, Victor Arias, James H A Clubb, Mirte van der Heijden, Teijo Pellinen, Joao M Santos

“…More substantial lymphocyte count change was linked to stronger immune activation in plasma proteome after intravenous TILT-123 and the presence of TILT-123 mRNA in tumors. Regarding other assays. tumor biopsies profiled showed increased amounts of CD8+ T cells, CD4+ T cells and NK cells after intravenous TILT-123, but not after intratumoral TILT-123. …”
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Steatohepatitis-induced vascular niche alterations promote melanoma metastasis by Johannes Hoffmann, Julia Schüler, Bianca Dietsch, Sina Wietje Kürschner-Zacharias, Carsten Sticht, Felix A. Trogisch, Maren Schreitmüller, Tinja Baljkas, Kai Schledzewski, Manuel Reinhart, Sebastian A. Wohlfeil, Manuel Winkler, Christian David Schmid, Joerg Heineke, Cyrill Géraud, Sergij Goerdt, Philipp-Sebastian Reiners-Koch, Victor Olsavszky

“…LSEC-specific GATA4 knockout mice (Gata4 LSEC−KO/BL) developing MASH-like liver fibrosis without steatosis via a pathogenic angiocrine switch were included to compare the impact of liver fibrosis versus hepatic steatosis on hepatic melanoma metastasis. Bulk RNA-Seq of isolated LSECs from CDAA-fed and control mice was performed. …”
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ROR1 CAR-T cells and ferroptosis inducers orchestrate tumor ferroptosis via PC-PUFA2 by Dan Li, Wenjie Zhang, Ruiheng Wang, Shufeng Xie, Yixin Wang, Wanxin Guo, Zixuan Huang, Chaoqun Lu, Liang Shan, Han Liu, Lifang Ma, Xumin Hou, Zhenshu Xu, Jiayi Wang

“…This study investigated the potential of combining receptor tyrosine kinase-like orphan receptor 1 (ROR1)-targeting CAR-T cells with ferroptosis inducers to promote ferroptosis of tumor cells and enhance anti-tumor efficacy. Methods RNA-seq data and immunofluorescence analysis of relapsed NSCLC patient samples were used to explore ROR1 expression. …”
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Isolation and Characterization of Indigenous <i>Acetobacter</i> Strains from Cashew Apple and Their Potential Use in Vinegar Production by Yaya Anianhou Ouattara, Doudjo Soro, Kouakou Romain Fossou, Ahou Cinthia Ines Yebouet, Ismael Abraham Cissé, Dogbo Marius Akissi, Adolphe Zézé, Emmanuel Assidjo, Kouassi Benjamin Yao

“…Physiological and biochemical tests followed by 16S rRNA gene analysis and phylogeny were used for isolate characterization. …”
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Bone Marrow Mesenchymal Stem Cells-Derived Exosomes Inhibit Apoptosis of Pulmonary Microvascular Endothelial Cells in COPD Mice Through miR-30b/Wnt5a Pathway by Song Q, Zhou A, Cheng W, Zhao Y, Liu C, Zeng Y, Lin L, Zhou Z, Peng Y, Chen P

“…Qing Song,1– 4 Aiyuan Zhou,5 Wei Cheng,1– 4 Yiyang Zhao,6 Cong Liu,1– 4 Yuqin Zeng,1– 4 Ling Lin,1– 4 Zijing Zhou,1– 4 Yating Peng,1– 4 Ping Chen1– 4 1Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, People’s Republic of China; 2Clinical Medical Research Center for Pulmonary and Critical Care Medicine in Hunan Province, Changsha, 410011, People’s Republic of China; 3Diagnosis and Treatment Center of Respiratory Disease, Central South University, Changsha, Hunan, 410011, People’s Republic of China; 4Research Unit of Respiratory Disease, Central South University, Changsha, Hunan, 410011, People’s Republic of China; 5Department of Respiratory and Critical Care Medicine, the Xiangya Hospital of Central South University, Changsha, Hunan, 410011, People’s Republic of China; 6Ultrasound Imaging Department, Xiangya Hospital of Central South University, Changsha, Hunan, 410083, People’s Republic of ChinaCorrespondence: Ping Chen, Department of Respiratory and Critical Care Medicine, the Second Xiangya Hospital, Central South University, Renmin Middle Road, Changsha, Hunan, 410011, People’s Republic of China, Tel 13873115563, Email pingchen0731@csu.edu.cnBackground: Bone marrow mesenchymal stem cells (BMSCs)-derived exosomes are rich in a variety of active substances, including microRNA (miR) and have shown powerful therapeutic effects to ameliorate cell injury and diseases. …”
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Biologically targeted discovery-replication scan identifies G×G interaction in relation to risk of Barrett’s esophagus and esophageal adenocarcinoma by Li Yan, Qianchuan He, Shiv P. Verma, Xu Zhang, Ann-Sophie Giel, Carlo Maj, Kathryn Graz, Elnaz Naderi, Jianhong Chen, Mourad Wagdy Ali, Puya Gharahkhani, Xiang Shu, Kenneth Offit, Pari M. Shah, Hans Gerdes, Daniela Molena, Amitabh Srivastava, Stuart MacGregor, Claire Palles, René Thieme, Michael Vieth, Ines Gockel, Thomas L. Vaughan, Johannes Schumacher, Matthew F. Buas

“…Rs3217992 maps to the CDKN2B 3′ UTR and reportedly disrupts microRNA-mediated repression. Rs17744726 maps to an intronic enhancer region in BLK. …”
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Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks by Cheng-long Jin, Sheng-lin Wang, Shuang Wang, Ya-nan Zhang, Wei-guang Xia, Chang Zhang, Xue-bing Huang, Kai-chao Li, Chun-tian Zheng, Wei Chen

“…We found the poor productive performance of aged ducks was correlated with follicle decreased numbers and atrophied microstructure, and restricted antioxidant ability of granulosa cells (GCs). Then, according to RNA-Seq analysis, we detected those aged ducks displayed lower Ca concentration in the ovary, while Ca channel related gene expression was increased in GCs to maintain homeostasis. …”
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Genome-Wide Analysis, Characterization, and Expression Profile of the Basic Leucine Zipper Transcription Factor Family in Pineapple by Yanhui Liu, Mengnan Chai, Man Zhang, Qing He, Zhenxia Su, S. V. G. N. Priyadarshani, Liping Liu, Guanxi Dong, Yuan Qin

“…The study also analyzed the pineapple gene expression of bZIP genes in different tissues, organs, and developmental stages, as well as in abiotic stress responses. The RNA-sequencing data showed that AcobZIP57 was upregulated in all tissues, including vegetative and reproductive tissues. …”
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Characteristics and clinical significance of immune cells in omental milky spots of patients with gastric cancer by Yasunobu Mano, Yasunobu Mano, Yuka Igarashi, Yuka Igarashi, Keisuke Komori, Keisuke Komori, Itaru Hashimoto, Itaru Hashimoto, Hayato Watanabe, Kosuke Takahashi, Kazuki Kano, Hirohito Fujikawa, Takanobu Yamada, Hidetomo Himuro, Hidetomo Himuro, Taku Kouro, Taku Kouro, Feifei Wei, Feifei Wei, Kayoko Tsuji, Kayoko Tsuji, Shun Horaguchi, Shun Horaguchi, Shun Horaguchi, Mitsuru Komahashi, Mitsuru Komahashi, Mitsuru Komahashi, Takashi Oshima, Tetsuro Sasada, Tetsuro Sasada

“…In this study, we investigated gene expression profiles in cells from omental milky spots of patients with gastric cancer (n = 37) by RNA sequencing analysis and classified the patients into four groups (G1-4). …”
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