Serum and tear autoantibodies from NOD and NOR mice as potential diagnostic indicators of local and systemic inflammation in Sjögren’s disease by Shruti Singh Kakan, Sara Abdelhamid, Sara Abdelhamid, Yaping Ju, Yaping Ju, J. Andrew MacKay, J. Andrew MacKay, J. Andrew MacKay, Maria C. Edman, Indu Raman, Chengsong Zhu, Prithvi Raj, Sarah F. Hamm-Alvarez, Sarah F. Hamm-Alvarez

“…Sources of Ig in tears were investigated using scRNA-Seq of the LG (GSE132420). Data were analyzed by R package Limma and Seurat.ResultsAnalysis of immunofluorescence in LG sections from both SjD models showed TLS. …”
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Time to HIV viral rebound and frequency of post-treatment control after analytical interruption of antiretroviral therapy: an individual data-based meta-analysis of 24 prospective... by Jesper D. Gunst, Jesal Gohil, Johanthan Z. Li, Ronald J. Bosch, Andrea White, Catherine Seamon, Tae-Wook Chun, Beatriz Mothe, Kathleen Gittens, Lauren Praiss, Marie-Angélique De Scheerder, Linos Vandekerckhove, Kevin Escandón, Ann Thorkelson, Timothy Schacker, Devi SenGupta, Christian Brander, Emmanouil Papasavvas, Luis J. Montaner, Javier Martinez-Picado, Ruxandra Calin, Antonella Castagna, Camilla Muccini, Wesley de Jong, Lorna Leal, Felipe Garcia, Rob A. Gruters, Timothy Tipoe, John Frater, Ole S. Søgaard, Sarah Fidler

“…In total, 24 eligible prospective studies with 382 individuals with ≥5 plasma HIV RNA viral loads (pVLs) within the first 84 days post-ATI were included. …”
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Treating drug-resistant tuberculosis in an era of shorter regimens: Insights from rural South Africa by J-D K Lotz, J Porter, H Conradie, T Boyles, B Gaunt, S Dimanda, D Cort

“…Injectables (adjusted odds ratio (aOR) 3.00, 95% confidence interval (CI) 1.48 - 6.09), bedaquiline (aOR 3.16, 95% CI 1.36 - 7.35), increasing age (aOR 0.97, 95% CI 0.95 - 0.99) and HIV viraemia defined as final HIV-RNA viral load >1 000 copies/mL (aOR 0.16, 95% CI 0.07 - 0.37) were all significantly and independently associated with treatment success. …”
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Microbial community variations in adult Hyalomma dromedarii ticks from single locations in Saudi Arabia and Tunisia by Myriam Kratou, Apolline Maitre, Apolline Maitre, Apolline Maitre, Lianet Abuin-Denis, Lianet Abuin-Denis, Rachid Selmi, Hanène Belkahia, Abdullah D. Alanazi, Hattan Gattan, Hattan Gattan, Bassam M. Al-Ahmadi, Abdullah F. Shater, Lourdes Mateos-Hernández, Dasiel Obregón, Lilia Messadi, Alejandro Cabezas-Cruz, Mourad Ben Said, Mourad Ben Said

“…Despite prior studies on its microbiota, the effects of geographic origin and sex on microbial community structure and functional stability remain poorly understood.MethodsTo address this, we characterized the bacterial microbiota of H. dromedarii ticks collected from camels in Tunisia (TUN) and Saudi Arabia (SA) using 16S rRNA gene sequencing, microbial network analysis, and metabolic pathway prediction.ResultsOur findings indicate a dominant presence of Francisella endosymbionts in Tunisian ticks, suggesting adaptive roles of H. dromedarii ticks in arid ecosystems. …”
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Assessment of prognosis and responsiveness to immunotherapy in colorectal cancer patients based on the level of immune cell infiltration by Kaili Liao, Minqi Zhu, Lei Guo, Zijun Gao, Jinting Cheng, Bing Sun, Yihui Qian, Bingying Lin, Jingyan Zhang, Tingyi Qian, Yixin Jiang, Yanmei Xu, Qionghui Zhong, Xiaozhong Wang

“…ObjectiveTo build a new prognostic risk assessment model based on immune cell co-expression networks for predicting overall survival and evaluating the efficacy of immunotherapy for colon cancer patients.MethodsThe Cancer Genome Atlas (TCGA) database was used to obtain mRNA expression profiling data, clinical information, and somatic mutation data from colorectal cancer patients. …”
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3-methyl-4-nitrophenol disturbs the maternal-to-zygotic transition of early embryos by damaging mitochondrial function and histone modification by Fan Chen, An-Feng Luo, Kai-Xin Pan, Hao Gu, Chang-Fan Zhou, Wei Zeng, Song Liu, Adrian Molenaar, Hong-Yan Ren, Li-Jun Huo, Yan-Zhen Bi

“…SMART-seq2 transcriptome sequencing further confirmed that PNMC treatment disrupted global gene expression in 2-cell embryos, with differentially expressed genes enriched in multiple signaling pathways, including those related to autophagy, apoptosis, fertilization, embryonic development, transcription, and mRNA processing. Integration of transcriptome data with open databases revealed that both zygotic genome activation genes and maternal factors experienced significant transcript-level disruptions. …”
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miR-129-5p Regulates the Immunomodulatory Functions of Adipose-Derived Stem Cells via Targeting Stat1 Signaling by He-Yang Zhang, Yu-Han Wang, Yan Wang, Yan-Nv Qu, Xiao-Hui Huang, Hui-Xin Yang, Chang-Ming Zhao, Youdi He, Si-Wei Li, Jin Zhou, Changyong Wang, Xiao-Xia Jiang

“…When fludarabine, an inhibitor of Stat1 activation, was added to ASCs with miR-129-5p knockdown, iNOS mRNA and protein levels were significantly reduced. …”
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Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study by Qiuling Wang, Qiuling Wang, Qiuling Wang, Lixin Zhang, Lixin Zhang, Lixin Zhang, Chenyuan Zhu, Chenyuan Zhu, Chenyuan Zhu, Ke Lu, Ke Lu, Ke Lu, Jiaqi Wu, Jiaqi Wu, Jiaqi Wu, Xu-Fang Liang, Xu-Fang Liang, Xu-Fang Liang

“…The average time to complete the trial in fosab−/− zebrafish was significantly higher than in WT zebrafish. The relative mRNA expression level of c-jun in fosab−/− zebrafish was significantly higher than that in WT zebrafish, while the fosaa−/− zebrafish has no discernible trend. …”
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Single‐Nucleus and Spatial Transcriptome Profiling Delineates the Multicellular Ecosystem in Hepatocellular Carcinoma After Hepatic Arterial Infusion Chemotherapy by YeXing Huang, ZeFeng Du, ZhiCheng Lai, DongSheng Wen, LiChang Huang, MinKe He, ZiChao Wu, HuiFang Li, HanYue OuYang, WenChao Wu, Anna Kan, Ming Shi

“…Here, we collected tumor samples from treatment‐naïve primary and post‐HAIC HCC, and integrated single‐nucleus RNA sequencing with spatial transcriptomics to characterize the tumor ecosystem in the post‐HAIC HCC. …”
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DNGR-1 limits Flt3L-mediated antitumor immunity by restraining tumor-infiltrating type I conventional dendritic cells by Ignacio Melero, Ruth Conde-Garrosa, David Sancho, Santos Mañes, Alfonso R Sanchez-Paulete, Francisco J Cueto, Carlos del Fresno, Paola Brandi, Alexis J. Combes, Elena Hernández-García, Michel Enamorado, Christian P Bromley, Manuel J Gomez, Santiago Zelenay, Salvador Iborra, Matthew F. Krummel

“…To characterize the immune response, we performed flow cytometry and RNA-Seq of tumor-infiltrating cDC1s.Results Here, we found that cross-presentation of tumor antigens in the steady state was DNGR-1-independent. …”
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Ibuprofen reduces inflammation, necroptosis and protects photoreceptors from light-induced retinal degeneration by Ping-Wu Zhang, Zi-He Wan, Weifeng Li, Abhishek Vats, Kunal Mehta, Laura Fan, Lingli Zhou, Sean Li, Gloria Li, Casey J. Keuthan, Cynthia Berlinicke, Cheng Qian, Noriko Esumi, Elia J Duh, Donald J. Zack

“…Twenty-four hour after LD, retinal mRNA expression for the inflammatory-factors tumor necrosis factor (Tnf), interleukin-1 beta (Il1B), and C-C motif chemokine ligand 2 (Ccl2) increased by 10-, 17-, and 533-fold, respectively; in IBU-treated animals, the expression levels of these inflammatory factors were not significantly different from no-LD controls. …”
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Influence of injection technique, drug formulation and tumor microenvironment on intratumoral immunotherapy delivery and efficacy by Patrick Hwu, Ravi Murthy, Aung Naing, Sapna P. Patel, Simon Young, Alda Tam, Nina M. Muñoz, Malea Williams, Katherine Dixon, Crystal Dupuis, Amanda McWatters, Rony Avritscher, Soraya Zorro Manrique, Kevin McHugh, David Leach, Jeffrey D. Hartgerink, Punit Prakash, Rahul A. Sheth

“…The influence of varying needle design and hydrogel-based drug delivery on the immune response to a stimulator of interferon genes (STING) agonist was evaluated using flow cytometry and single cell RNA sequencing. We also evaluated the influence of tumor stiffness on drug injection distribution.Results Variations in needle design, specifically with the use of a multiside hole needle, led to approximately threefold improvements in intratumoral drug deposition relative to conventional end-hole needles. …”
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Stratification system with dual human endogenous retroviruses for predicting immunotherapy efficacy in metastatic clear-cell renal cell carcinoma by Li Xu, Stephane Oudard, Catherine Sautes-Fridman, Xiaoping Su, Gabriel G. Malouf, Virginie Verkarre, Salma Kotti, Fangrong Yan, Yann-Alexandre Vano, Wolf Herve Fridman, Cheng-Ming Sun, Xiaofan Lu, Wenxuan Cheng

“…ERV expression was quantified using the ERVmap algorithm from RNA sequencing data. Our primary objective was to correlate ERV expression with progression-free survival, with overall survival and time-to-second-treatment survival as secondary endpoints. …”
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Understanding the Molecular Mechanisms of Incomptine A in Treating Non-Hodgkin Lymphoma Associated with U-937 Cells: Bioinformatics Approaches, Part I by Fernando Calzada, Normand García-Hernández, Elihú Bautista, José Manuel Sánchez-López, Miguel Valdes, Claudia Velázquez, Elizabeth Barbosa

“…Gene ontology, Reactome, and KEGG pathway analysis showed that proteins with altered levels may be implicated in several processes, including gene silencing by RNA, oxidative phosphorylation, glycolysis/gluconeogenesis, cytoskeleton organization, and ATP metabolic and energetic processes. …”
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Field-based evidence for the prevalence of soil antibiotic resistomes under long-term antibiotic-free fertilization by Yuhao Fu, Fang Hu, Fang Wang, Min Xu, Zhongjun Jia, Wulf Amelung, Zhi Mei, Xiaozeng Han, Marko Virta, Xin Jiang, James M. Tiedje

“…Long-term fertilization slightly impacts the diversity of antibiotic resistomes, with 85.5 % of total ARGs and mobile genetic elements (MGEs) being common across all treatment types, while significantly increasing their abundances from 0.68 to a maximum of 0.90 copies/16S rRNA. The rise in ARG abundances was less pronounced when using antibiotic-free manure compared to chemical fertilizer, particularly for Rank Ⅱ ARGs. …”
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Folic acid alleviates the negative effects of dexamethasone induced stress on production performance in Hyline Brown laying hens by Xi Sun, Chaohui Wang, Sijing Li, Xiaoying Liu, Yun Li, Yumeng Wang, Yuxin Niu, Zhouzheng Ren, Xin Yang, Xiaojun Yang, Yanli Liu

“…Kyoto Encyclopedia of Genes and Genomes enrichment from the transcriptome and metabolomics showed that the reduced production performance may be due to the disturbance of oocyte production, calcium metabolism, and oxidative stress. Analysis of 16S rRNA gene amplicon sequences revealed the differential microbial composition and potential functional changes among the different groups. …”
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Identification and validation of a prognostic signature of drug resistance and mitochondrial energy metabolism-related differentially expressed genes for breast cancer by Tiankai Xu, Chu Chu, Shuyu Xue, Tongchao Jiang, Ying Wang, Wen Xia, Huanxin Lin

“…Furthermore, we examined immune infiltration and tumor mutation burden of the genes within our model and scrutinized divergences in the immune microenvironment between high- and low-risk groups. Small hairpin RNA and lentiviral plasmids were designed for stable transfection of breast cancer cell lines MDA-MB-231 and HCC1806. …”
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L-glutaminase synthesis by Klebsiella pneumoniae (AS KP 23) isolated from clinical strain, and its efficacy against human hepatocellular and breast cancer cell lines by Lina J. Abdel-Hafez, Eman Y. T. Elariny, Asmaa E. Ibrahim, Mahmoud E. F. Abdel-Haliem

“…Results In this study, a bacterium extracellular from human stool samples was extracted and identified using morphological, biochemical, and molecular methods. The 16 S rRNA gene was 100% identical to the sequence from Klebsiella pneumoniae and was submitted to GenBank under accession number OQ703039. …”
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Whole blood transcriptome profiles of trypanotolerant and trypanosusceptible cattle highlight a differential modulation of metabolism and immune response during infection by Trypan... by Peylhard, Moana, Berthier, David, Dayo, Guiguigbaza-Kossigan, Chantal, Isabelle, Sylla, Souleymane, Nidelet, Sabine, Dubois, Emeric, Martin, Guillaume, Sempéré, Guilhem, Flori, Laurence, Thévenon, Sophie

“…To this end, whole blood genome-wide transcriptome of three trypanotolerant taurine breeds (N’Dama, Lagune and Baoulé), one susceptible zebu (Zebu Fulani) and one African taurine x zebu admixed breed (Borgou) were profiled by RNA sequencing at four time points, one before and three during infection. …”
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The mechanism of tea tree oil regulating the damage of hydrogen sulfide to spleen and intestine of chicken by Yachao Wang, Yilei Liang, Yan Huang, Wei wang, Xiaoyan Long, Li Jiang, Tingting Cheng, Jinfeng Du, Xuegang Luo

“…Intestinal findings indicated that TTO treatment enhanced villus height, reduced crypt depth, and upregulated the expression of Claudin 1, Occludin, and ZO-1 mRNA in the jejunum (P < 0.05). After TTO treatment, H2S-induced oxidative stress injury and apoptosis protein expression in spleen were improved (P < 0.05). …”
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