Development and validation of a novel artificial intelligence algorithm for precise prediction the postoperative prognosis of esophageal squamous cell carcinoma by Zichen Wang, Zhihan Xiao, Tongyu Zhang, Meiyou Lu, Hai Li, Jing Cao, Jianan Zheng, Yichan Zhou, Juncheng Dai, Cheng Wang, Liang Chen, Jing Xu

“…Then we systematically screen piRNAs associated with esophageal carcinoma based on GWAS, eQTL-piRNA, and i2OM databases, and successfully discovered 8 piRNAs potentially regulated by HENMT1. …”
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Alleviating heat stress on broiler breeder hens: Effect of dietary antioxidant supplementation on reproductive performance, egg quality, offspring growth, and antioxidant capacity by Phocharapon Pasri, Chayanan Pukkung, Pascal Mermillod, Nadine Gérard, Orapin Jantasaeng, Merisa Sirisopapong, Supattra Okrathok, Thanatat Thiabching, Sitthipong Rakngam, Kanokwan Kamkajon, Yongcai Zhu, Sutisa Khempaka

“…Either antioxidant source can improve free radical scavenging, reduce lipid peroxidation, upregulate the relative expression of SOD, CAT, and GSH-Px mRNA, and downregulate HSP90 in offspring liver (P < 0.05). …”
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Sofosbuvir-Based Therapies for Patients with Hepatitis C Virus Infection: Real-World Experience in China by Chengguang Hu, Guosheng Yuan, Junwei Liu, Huaping Huang, Yanyu Ren, Yinping Li, Xuefu Chen, Wei Li, Tao Wu, Hong Deng, Yanzhong Peng, Yong-Yuan Zhang, Yuanping Zhou

“…No significant differences in efficacy were detected among different regimens (93% versus 97% versus 100%, respectively, P=0.153), gender (95% for male versus 96% for female, P=0.655), or baseline HCV RNA lever (96% versus 95%, respectively, P=0.614). …”
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N1-methylnicotinamide promotes age-related cochlear damage via the overexpression of SIRT1 by Toru Miwa, Toru Miwa, Akihito Tarui, Akihito Tarui, Teppei Kouga, Yasunori Asai, Yasunori Asai, Hideaki Ogita, Hideaki Ogita, Taro Fujikawa, Nobuhiro Hakuba, Nobuhiro Hakuba

“…MNAM increased SIRT1 protein levels in the cochlea without altering Sirt1 mRNA, suggesting post-transcriptional regulation. …”
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Cardio-renal effect of dapagliflozin and dapagliflozin- saxagliptin combination on CD34 + ve hematopoietic stem cells (HSCs) and podocyte specific markers in type 2 diabetes (T2DM)... by Seshagiri Rao Nandula, Arad Jain, Sabyasachi Sen

“…CD34 + HSC number, migration, mRNA expression along with biochemistry and urine exosomes were measured. …”
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Malaria prevalence, transmission potential and efficacy of artemisinin-based combination therapy in the Kenyan Central highlands: a zone previously characterized as malaria-free by Francis T. Kimani, Kelvin K. Thiongó, Maureen A. Otinga, Lewis K. Mbabu, Mary N. Ombati, Stanley K. Kitur, Sarah A. Ochieng’, Lucy N. Wachira, Damaris K. Matoke-Muhia, Luna Kamau

“…Microscopy was used at the facility while nested 18 s rRNA subunit gene PCR amplification and MSP-1 and MSP-2 family alleles genotyping was done in the laboratory. …”
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Equine piroplasmosis in different geographical areas in France: Prevalence heterogeneity of asymptomatic carriers and low genetic diversity of Theileria equi and Babesia caballi by Maggy Jouglin, Claire Bonsergent, Nathalie de la Cotte, Mickaël Mège, Céline Bizon, Anne Couroucé, Élodie-Anne Lallemand, Agnès Leblond, Louise C Lemonnier, Aurélia Leroux, Ilaria Marano, Alexandre Muzard, Émilie Quéré, Marion Toussaint, Albert Agoulon, Laurence Malandrin

“…We developed a species-specific nested PCR protocol targeting the 18S small sub-unit (SSU) rRNA gene and used it on blood samples collected from 566 asymptomatic horses across four National Veterinary Schools.The carrier frequency varied from 18.7 % around Paris (central-north) to 56.1 % around Lyon (southeast), with an overall prevalence of 38.3 %. …”
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Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening by Wei Jin, Yexuan Deng, John E. La Marca, Emily J. Lelliott, Sarah T. Diepstraten, Christina König, Lin Tai, Valentina Snetkova, Kristel M. Dorighi, Luke Hoberecht, Millicent G. Hedditch, Lauren Whelan, Geraldine Healey, Dan Fayle, Kieran Lau, Margaret A. Potts, Moore Z. Chen, Angus P. R. Johnston, Yang Liao, Wei Shi, Andrew J. Kueh, Benjamin Haley, Jean-Philippe Fortin, Marco J. Herold

“…Our enAsCas12a mouse and accompanying crRNA libraries enhance genome engineering capabilities and complement current CRISPR technologies.…”
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Targeting early tau pathology: probiotic diet enhances cognitive function and reduces inflammation in a preclinical Alzheimer’s model by Cassandra M. Flynn, Tamunotonye Omoluabi, Alyssa M. Janes, Emma J. Rodgers, Sarah E. Torraville, Brenda L. Negandhi, Timothy E. Nobel, Shyamchand Mayengbam, Qi Yuan

“…Probiotic or control diets were administered at 9–12 months, with blood and fecal samples collected for ELISA and 16S rRNA gene sequencing. Behavioral assessments were conducted at 13–14 months, followed by analysis of brain inflammation, blood-brain barrier integrity, and GSK-3β activation. …”
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The expression landscape and clinical significance of methyltransferase-like 17 in human cancer and hepatocellular carcinoma: a pan-cancer analysis using multiple databases by Yezhou Ding, Mingyang Feng, Wanqing Chi, Xiaoyin Wang, Baoyan An, Kehui Liu, Shike Lou, Xiaolin Wang, Hui Wang

“…First, we comprehensively analyzed RNA-seq data from the HPA database of 25 human tissues. …”
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FNDC5/irisin mitigates the cardiotoxic impacts of cancer chemotherapeutics by modulating ROS-dependent and -independent mechanisms by Manish Kumar, Abhishek Singh Sengar, Anushree Lye, Pranesh Kumar, Sukhes Mukherjee, Dinesh Kumar, Priyadip Das, Suvro Chatterjee, Adele Stewart, Biswanath Maity

“…Whereas FNDC5 overexpression in murine heart was cardioprotective, introduction of FNDC5-targeted shRNA into the myocardium was sufficient to trigger Bax up-regulation, ATR/Chk1 activation, oxidative stress, cardiac fibrosis, loss of ventricular function, and compromised animal survival. …”
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Integrating rapamycin with novel PI3K/Akt/mTOR inhibitor microRNAs on NOTCH1-driven T-cell acute lymphoblastic leukemia (T-ALL) by Fateme Arjmand, Samaneh Shojaei, Mitra Khalili, Hossein Dinmohammadi, Behzad Poopak, Samira Mohammadi-Yeganeh, Yousef Mortazavi

“…The Jurkat cell line was chosen as a representative model for T-ALL. Gene and miRNA expression levels were assessed using quantitative real-time PCR (qRT-PCR). …”
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Daily feeding frequency impacts muscle characteristics and fat deposition in finishing pigs associated with alterations in microbiota composition and bile acid profile by Luga Hu, Huayu Tang, Zhaoxi Xie, Hongyu Yi, Lunjie Feng, Pan Zhou, Yong Zhang, Jingbo Liu, Xiang Ao, Jianchuan Zhou, Honglin Yan

“…The muscle fiber characteristics, lipid metabolism in adipose tissue, and cecal microbiota and bile acid composition were determined.ResultsThe present study revealed that pigs fed four meals exhibited a lower feed-to-gain ratio, abdominal fat weight, and average backfat thickness (p &lt; 0.05), as well as a higher loin eye area (p = 0.09) and myofiber diameter in the longissimus muscle than their counterparts. The mRNA expression of slow-twitch fiber and myogenesis-associated genes in the longissimus muscle was upregulated, while lipid metabolism-related genes in the backfat were downregulated in the M4 group compared to the M2 group (p &lt; 0.05). …”
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Anti-inflammatory coupled anti-angiogenic airway stent effectively suppresses tracheal in-stents restenosis by Yanan Zhao, Yiming Liu, Jiheng Shan, Xiaohong Xu, Chengzhi Zhang, Zaoqu Liu, Xiaomeng Li, Zhaoqian Zhong, Yanxia Gao, Kewei Ren, Dechao Jiao, Jianzhuang Ren, Ping Wu, Yong Jiang, Xinwei Han

“…PAGL was implanted into the tracheae of New Zealand rabbits to evaluate its efficacy in inhibiting bacterial infection, suppressing the inflammatory response, reducing angiogenesis, and attenuating excessive fibroblast activation. RNA sequencing analysis revealed a significant downregulation of genes associated with fibrosis, intimal hyperplasia, and cell migration following PAGL treatment. …”
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MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells by Daigaku Hasegawa, Kana Hasegawa, Hiroshi Kaneko, Shinichiro Yoshida, Hiromi Mitarai, Mai Arima, Atsushi Tomokiyo, Sayuri Hamano, Hideki Sugii, Naohisa Wada, Tamotsu Kiyoshima, Hidefumi Maeda

“…Knockdown of MEST by siRNA in 2-23 cells inhibited the expression of stem cell markers, such as CD105, CD146, p75NTR, N-cadherin, and NANOG; the proliferative potential; and multidifferentiation capacity for osteoblasts, adipocytes, and chondrocytes. …”
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Expression and Significances of Contactin-1 in Human Gastric Cancer by Ji-Wei Yu, Sheng-Hua Wu, Rui-qi Lu, Ju-gang Wu, Xiao-Chun Ni, Gou-cai Zhou, Hai-guang Jiang, Lin-Hai Zheng, Xiao-Qiang Li, Guang-Ye Du, Bo-Jian Jiang

“…The expression level of CNTN-1 mRNA and CNTN-1 protein of 33 cases was determined using RT-PCR and Western Blot. …”
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Personalized prediction of glycemic responses to food in women with diet-treated gestational diabetes: the role of the gut microbiota by Polina V. Popova, Artem O. Isakov, Anastasiia N. Rusanova, Stanislav I. Sitkin, Anna D. Anopova, Elena A. Vasukova, Alexandra S. Tkachuk, Irina S. Nemikina, Elizaveta A. Stepanova, Angelina I. Eriskovskaya, Ekaterina A. Stepanova, Evgenii A. Pustozerov, Maria A. Kokina, Elena Y. Vasilieva, Lyudmila B. Vasilyeva, Soha Zgairy, Elad Rubin, Carmel Even, Sondra Turjeman, Tatiana M. Pervunina, Elena N. Grineva, Omry Koren, Evgeny V. Shlyakhto

“…Machine learning models were created using CGM data, meal content, lifestyle factors, biochemical parameters, and microbiota data (16S rRNA gene sequence analysis). Adding microbiome data increased the explained variance in peak glycemic levels (GLUmax) from 34 to 42% and in incremental area under the glycemic curve (iAUC120) from 50 to 52%. …”
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The effects of Lepidium meyenii on MSG-induced subfertility in male Wistar rats: Targeting oxidative stress, pro-inflammatory cytokines, steroidogenic enzymes, and reproductive hor... by Temidayo S. Omolaoye, Surendra Singh Rawat, Naglaa Gamil Shehab, Eslam El Nebrisi, Stefan S. Du Plessis

“…Results: MSG administration significantly reduced total and progressive sperm motility (p < 0.05), decreased total antioxidant capacity (TAC) (p < 0.001), increased mRNA expression of TNF-α, IL-6, IL-1β in the testis and prostate (p < 0.05), protein expression of GP130 in the testis, reduced serum testosterone, and altered expression of steroidogenic markers. …”
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Polyethylene microplastic exposure adversely affects oocyte quality in human and mouse by Qiaoling Wang, Fengli Chi, Yingdong Liu, Qiurong Chang, Siyu Chen, Pengcheng Kong, Wanli Yang, Wenqiang Liu, Xiaoming Teng, Yan Zhao, Yi Guo

“…In mice, PE exposure significantly reduced the number of retrieved oocytes (31.5 vs. 36.3, P < 0.05) and fertilization rate (70.8 % vs. 85.2 %, P < 0.001), while increasing the proportion of poor-quality oocytes (28.2 % vs. 16.5 %, P < 0.001) and reactive oxygen species (ROS) production compared to controls. RNA sequencing indicated significant upregulation of inflammation-related genes (Il10ra, Il1a, Il33, Tnfaip8l2, and Tnfrsf1b) in the PE-exposed group. …”
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Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines by Anton Wellstein, Thomas Seufferlein, Alexander Kleger, Tim Eiseler, Anton Lahusen, Nora Minhöfer, Kim-André Lohse, Christine Blechner, Jessica Lindenmayer, Sabine Windhorst, Yuan-Na Lin

“…We investigated their contribution by shRNA-mediated knockdown and pharmacological inhibition in murine in vitro and in vivo studies, as well as in patient-derived organoids (PDOs). …”
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