New bitongling regulates gut microbiota to predict angiogenesis in rheumatoid arthritis via the gut-joint axis: a deep neural network approach by Yin Guan, Xiaoqian Zhao, Yun Lu, Yue Zhang, Yan Lu, Yue Wang

“…The findings were further validated through analysis with deep neural network models and in vivo/in vitro experiments, including western blot, immunofluorescence, and miRNA analysis.ResultsNBTL treatment markedly diminished inflammation in RA rats, evidenced by the reduced expression of TNF-α, IL-17, IL-6, and ASC in synovial tissues. …”
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Combined therapy of human amnion-derived mesenchymal stem cells and scalp acupuncture alleviates brain damage in a rat model of cerebral palsy by Yu Zhou, Xu-Huan Li, Lu-Na He, Li-Na Wang, Jing Zang, Dong-Ming Wang, Jing Gao, Xue-feng Yu

“…The combination therapy further decreased apoptosis levels, normalized mRNA expression of Bax, Caspase 9, and Caspase 3, and alleviated histological damage. …”
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Yunvjian decoction attenuates lipopolysaccharide-induced acute lung injury by inhibiting NF-κB/NLRP3 pathway and pyroptosis by Fanxuan Zhang, Fang Wang, Lisha Zhao, Leqian Wang, Wenjing Li, Feihua Huang, Feihua Huang, Nani Wang, Nani Wang

“…The mechanism of action of YNJ on these model systems was studied using RNA sequencing, immunohistochemical analysis, immunoblotting, immunofluorescence, ELISA, and polymerase chain reaction assays. …”
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Inhibition of glutathione peroxidase 4 suppresses gastric cancer peritoneal metastasis via regulation of RCC2 homeostasis by Can Hu, Jingli Xu, Yanqiang Zhang, Ruolan Zhang, Siwei Pan, Jiahui Chen, Yan Wang, Qianyu Zhao, Yuqi Wang, Weiwei Zhu, Mengxuan Cao, Shengjie Zhang, Dan Zu, Zhiyuan Xu, Ji Jing, Xiangdong Cheng

“…In this study, we found that GPx4 was overexpressed and negatively associated with poor prognosis and distant metastasis, as confirmed by single-cell RNA sequencing (scRNA-seq) and validation with retrospective clinical samples. …”
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Variability of plankton communities in relation to the lunar cycle in oceanic waters by Santiago Hernández-León, M. Loreto Torreblanca, M. Loreto Torreblanca, Inma Herrera, Inma Herrera, Laia Armengol, Gara Franchy, Alejandro Ariza, Juan Carlos Garijo, María Couret

“…Zooplankton abundance and biomass, gut fluorescence (GF), electron transfer system (ETS), and aminoacyl-tRNA synthetase (AARS) activities were measured before, during, and after the winter vertical mixing in these waters in a wide range of size classes. …”
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NUDT21 regulates lysyl oxidase-like 2(LOXL2) to influence ECM protein cross-linking in silica-induced pulmonary fibrosis by Lan Peng, Wenqing Sun, Demin Cheng, Xinying Jia, Wenxiu Lian, Ziwei Li, Haojie Xiong, Ting Wang, Yi Liu, Chunhui Ni

“…Furthermore, we discovered that NUDT21, the LOXL2 upstream regulatory mechanism of LOXL2, alters LOXL2's 3′UTR usage by substituting alternative polyadenylation (APA), thereby modulating LOXL2 expression. By injecting LOXL2 siRNA-loaded liposomes into the tail vein of mice in the silica dust-treated mouse pulmonary fibrosis model, the severity of lung fibrosis was significantly reduced. …”
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The Positive Regulatory Effect of DBT on Lipid Metabolism in Postpartum Dairy Cows by Zheng Zhou, Kang Yong, Zhengzhong Luo, Zhenlong Du, Tao Zhou, Xiaoping Li, Xueping Yao, Liuhong Shen, Shumin Yu, Yixin Huang, Suizhong Cao

“…Cows in the H-DBT group and control group were selected for metabolome and 16S rRNA amplicon sequencing. Results: Our research shows that, in dairy cows 7 days postpartum, DBT significantly reduced serum 3-hydroxybutyric acid (BHB) concentrations and the number of cows with BHB concentrations ≥ 1 mmol/L. …”
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Cellular and immune landscape of chronic liver diseases: insights from immunophenotyping by Shaghayegh Soleimani, Shaghayegh Soleimani, Ozgur Albayrak, Kayra Somay, Hong Yang, Buket Yigit, Burge Ulukan, Dila Atak, Murat Akyildiz, Metehan Gursoy, Elif Demirtas, Adil Mardinoglu, Adil Mardinoglu, Atay Vural, Atay Vural, Murat Dayangac, Mujdat Zeybel

“…This study aimed to explore the immune cell characteristics of the clinical conditions.MethodsExplant liver samples were collected from 25 patients for bulk RNA sequencing and flow cytometry analysis. Immune cell populations were characterized by flow cytometry from isolated hepatic and peripheral mononuclear cells.ResultsSignificant differences in immune cell characteristics were observed among patients with three clinical conditions. …”
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Assessment of upper respiratory and gut bacterial microbiomes during COVID-19 infection in adults: potential aerodigestive transmission by Hafez Al-Momani, Andrew Nelson, Hadeel Al Balawi, Dua’a Al Balawi, Iman Aolymat, Ashraf I. Khasawneh, Hala Tabl, Ayman Alsheikh, AbdelRahman M. Zueter, Jeffrey Pearson, Christopher Ward

“…DNA sequencing of the V4 region of the 16 S rRNA gene was performed for microbiome analysis. …”
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Discovery of anti-Ebola virus multi-target inhibitors from traditional Chinese medicine database using molecular screening, biophysical investigation, and binding free energy calcu... by Abbas Khan, Abrar Mohammad Sayaf, Anwar Mohammad, Fahad M. Alshabrmi, Tarek Benameur, Dong-Qing Wei, Kar Kheng Yeoh, Abdelali Agouni

“…Introduction: Ebola virus (EBOV) is a highly lethal RNA virus that causes severe hemorrhagic fever in humans and non-human primates. …”
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Hyperosmotic Stress Induces a Specific Pattern for Stress Granule Formation in Human-Induced Pluripotent Stem Cells by Salam Salloum-Asfar, Rudolf Engelke, Hanaa Mousa, Neha Goswami, I. Richard Thompson, Freshteh Palangi, Kamal Kamal, Muna N. Al-Noubi, Frank Schmidt, Sara A. Abdulla, Mohamed M. Emara

“…Stress granules (SGs) are assemblies of selective messenger RNAs (mRNAs), translation factors, and RNA-binding proteins in small untranslated messenger ribonucleoprotein (mRNP) complexes in the cytoplasm. …”
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Roles of the rpoEc-chrR-chrA operon in superoxide tolerance and β-lactam susceptibility of Stenotrophomonas maltophilia by Ren-Hsuan Ku, Hsu-Feng Lu, Li-Hua Li, Li-Hua Li, Ting-Yu Yeh, Yi-Tsung Lin, Yi-Tsung Lin, Tsuey-Ching Yang

“…The contribution of rpoEc-chrR-chrA operon to oxidative stress alleviation and antibiotic susceptibility was evaluated using mutant constructs and stress-tolerance assays. RNA-seq transcriptome assay of wild-type KJ, KJΔChrR (chrR mutant), and KJΔChrRΔRpoEc (chrR/rpoEc double mutant) was performed to reveal the σEc regulon.ResultsThe rpoEc-chrR pair and downstream chrA formed an operon. …”
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Free fatty acids may regulate the expression of 11β-hydroxysteroid dehydrogenase type 1 in the liver of high-fat diet golden hamsters through the ERS-CHOP-C/EBPα signaling pathway... by Dandan Liu, Peipei Tian, Yilin Hou, Tingxue Zhang, Xiaoyu Hou, Lifang Liu, Xiaolong Li, Kunjie Zheng, Chao Wang, Guangyao Song

“…Western blot and RT-PCR results indicated higher GRP78, CHOP, C/EBPα, and 11β-HSD1 protein and mRNA expression in the HF group compared to the Con group (Ps < 0.05). …”
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Development of the novel gene chip and restriction fragment length polymorphism (RFLP) methods for rapid detection of Mycobacterium tuberculosis complex in broth culture by Wen-Hung Wang, Chun-Yu Lin, Shu-Huei Jain, Po-Liang Lu, Yen-Hsu Chen

“…Methods: We assessed the effectiveness of a novel gene chip and RFLP methods targeting the 16S rRNA gene of Mycobacterium tuberculosis in 2000 MGIT culture-positive specimens. …”
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Identification of Potential Diagnostic Biomarkers of Carotid Atherosclerosis in Obese Populations by Wu X, Pan J, Pan X, Kang J, Ren J, Huang Y, Gong L, Li Y

“…In vivo experiments showed that MMP9, PLA2G7, CD52, SPP1, FYB, and PLCB2 mRNA levels were up-regulated in adipose, aortic tissues and serum of OB and AS model mice, CLEC5A was up-regulated in aorta and serum, and PRKCB was up-regulated in adipose and serum.Conclusion: MMP9, PLA2G7, CD52, CLEC5A, SPP1, and FYB may serve as potential diagnostic biomarkers for CAS in obese populations. …”
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Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China by Jingge Ma, Jingge Ma, Zhiwei Wei, Liang Li, Wei Wang, Ziyan Liu, Ziyan Liu, Ning Liu, Feng Wei, Xiangyu Zheng, Zedong Wang

“…However, the genetic diversity, geographical distribution, and host range of the virus remains poorly understood.MethodsIn this study, serum samples from cattle and sheep were obtained in Hulunbuir and pooled to establish RNA libraries, which were then analyzed using transcriptome sequencing. …”
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A new signature associated with anoikis predicts the outcome and immune infiltration in nasopharyngeal carcinoma by Yonglin Luo, Wenyang Wei, Yaxuan Huang, Jun Li, Weiling Qin, Quanxiang Hao, Jiemei Ye, Zhe Zhang, Yushan Liang, Xue Xiao, Yonglin Cai

“…Moreover, the expression of RBL2, E2F1, and CDC25C were significantly correlated with the expression of caspase family genes. Finally, the lower mRNA and protein expression of RBL2 were validated in NPC cell lines and tissues. …”
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Targeting sphingosine 1-phosphate receptor 3 inhibits T-cell exhaustion and regulates recruitment of proinflammatory macrophages to improve antitumor efficacy of CAR-T cells agains... by Weiting Liao, Ge Gao, Xia He, Pei Shu, Qizhi Ma, Benxia Zhang, Diyuan Qin, Yongsheng Wang

“…However, the functional significance of S1PR3 in T-cell-based immunotherapies and the molecular mechanisms have not been fully understood.Methods Here, we studied the combination of EpCAM-specific CAR T-cell therapy with pharmacological blockade of S1PR3 against solid tumor. We have applied RNA sequencing, flow cytometry, ELISA, cellular/molecular immunological technology, and mouse models of solid cancers.Results Our study provided evidence that S1PR3 high expression is positively associated with resistance to programmed cell death protein-1 (PD-1)-based immunotherapy and increased T-cell exhaustion. …”
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Efficacy of yeast beta-glucan 1,3/1,6 supplementation on respiratory infection, fatigue, immune markers and gut health among moderate stress adults in Klang Valley of Malaysia: pro... by Suzana Shahar, Kalavathy Ramasamy, Norhayati Ibrahim, Nur Nadia Mohamad Habibullah, Munirah Ismail, Mohd Zul Amin Kamaruddin, Shirley Gee Hoon Tang, Mohd Faisal Abdul Hamid

“…Secondary outcome includes gut microbiota analysis using stool samples via 16S rRNA sequencing.Ethics and dissemination The research protocol of the study was reviewed and approved by the Research Ethics Committee of Universiti Kebangsaan Malaysia (UKM/PPI/111/8/JEP-2023–211). …”
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hUC-MSC preserves erectile function by restoring mitochondrial mass of penile smooth muscle cells in a rat model of cavernous nerve injury via SIRT1/PGC-1a/TFAM signaling by Mengbo Yang, Xinda Chen, Ming Zhang, Xiaolin Zhang, Dongdong Xiao, Huiming Xu, Mujun Lu

“…To explore the role of the SIRT1/PGC-1α/TFAM pathway in regulating mitochondrial biogenesis of CCSMCs, we knocked down SIRT1 by siRNA. Results The administration of hUC-MSC significantly improved erectile function of CNI-ED rats and reduced the ratio of collagen to smooth muscle. …”
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