Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

“…The most potent inhibitor was evaluated in vivo in two animal models and the mechanisms of action were investigated by RNA sequencing, Western blotting and immunofluorescence staining. …”
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Expression of Sclerostin in Osteoporotic Fracture Patients Is Associated with DNA Methylation in the CpG Island of the SOST Gene by Yanming Cao, Bin Wang, Ding Wang, Dongxiang Zhan, Caiyuan Mai, Peng Wang, Qiushi Wei, Yamei Liu, Haibin Wang, Wei He, Liangliang Xu

“…After extracting total RNA and protein, we checked the relative expression levels of SOST by quantitative real-time PCR and western blot. …”
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Association of Adult Atopic Dermatitis with Impaired Oral Health and Oral Dysbiosis: A Case-Control Study by Aaya Shahin, Yael Anne Leshem, Yossi Taieb, Sharon Baum, Aviv Barzilai, Danielle Jeddah, Efrat Sharon, Omry Koren, Rinat Tzach-Nahman, Shunit Coppenhagen-Glazer, Ronen Hazan, Yael Houri-Haddad, Shoshana Greenberger

“…A dentist assessed oral health status, and oral flora samples were collected and subjected to 16S rRNA sequencing for microbiome analysis. Results: Forty-five AD participants and 41 non-AD controls were recruited. …”
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Class III β-Tubulin Overexpression Induces Chemoresistance to Eribulin in a Leiomyosarcoma Cell Line by Kenichiro Yahiro, Yoshihiro Matsumoto, Jun-ichi Fukushi, Ken-ichi Kawaguchi, Makoto Endo, Nokitaka Setsu, Keiichiro IIda, Suguru Fukushima, Makoto Nakagawa, Atsushi Kimura, Yoshinao Oda, Yasuharu Nakashima

“…We found that class III β-tubulin (TUBB3) was overexpressed in the resistant cell line, and siRNA knockdown of TUBB3 partially recovered sensitivity to eribulin. …”
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Effects of Dietary Nano-Composite of Copper and Carbon on Antioxidant Capacity, Immunity, and Cecal Microbiota of Weaned Ira White Rabbits by Ying-Huan Zhou, Xiao-Ping Liu, Xiao-Ming Gu, Hai-Xuan Lv, Yun Yang, Zai-Xing Cai, Bin Di, Chang-Kang Wang, Yu-Yun Gao, Ling Jin

“…In addition, the metagenomic analysis of 16 S rRNA showed significant differences in the cecal microbial structure of weaned Ira white rabbits in the NCCC III group compared with the CON, NCCC I, and NCCC II groups (<i>p</i> < 0.05). …”
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Comprehensive pan-cancer analysis reveals NTN1 as an immune infiltrate risk factor and its potential prognostic value in SKCM by Fuxiang Luan, Yuying Cui, Ruizhe Huang, Zhuojie Yang, Shishi Qiao

“…Analysis of the TCGA, GTEx, and UALCAN databases revealed significant differences in NTN1 expression at both the mRNA and protein levels. Prognostic value was evaluated through univariate Cox regression and Kaplan–Meier methods. …”
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Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells by Peihua Zhang, Jin Li, Yawei Qi, Xudong Tang, Jianfeng Duan, Li Liu, Zeyong Wu, Jie Liang, Jiangfeng Li, Xian Wang, Guofang Zeng, Hongwei Liu

“…Therefore, TIMP-1 expression in the ADSCs was firstly detected by western blotting, and TIMP-1 gene was knocked down by lentivirus-mediated shRNA. Cell proliferation was then evaluated by MTT assay and Ki67 staining, respectively. …”
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Gal-3 activates Tyro3 to ameliorate ferroptosis of hippocampal neurons after traumatic brain injury by Xiao Zhang, Manrui Li, Yang Xu, Jingting Wu, Ruixuan Yuan, Yihan Sun, Xiaogang Chen, Meili Lv, Bo Jin, Xiameng Chen, Weibo Liang

“…Our bioinformatics analysis of single-cell RNA sequencing data from hippocampal tissue following TBI revealed persistent neuronal loss and activation of ferroptosis-related pathways. …”
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TRPV Subfamily (TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) Gene and Protein Expression in Patients with Ulcerative Colitis by Joel J. Toledo Mauriño, Gabriela Fonseca-Camarillo, Janette Furuzawa-Carballeda, Rafael Barreto-Zuñiga, Braulio Martínez Benítez, Julio Granados, Jesus K. Yamamoto-Furusho

“…TRPV5 had significantly higher mRNA levels in a control group compared with active UC patients (P=0.02). …”
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Lignans from Patrinia scabiosaefolia improve insulin resistance by activating PI-3K/AKT pathway and promoting GLUT4 expression by Zhenhua Liu, Mengke Wang, Lijun Meng, Yixiao Chen, Qiuyi Wang, Yan Zhang, Xuefeng Xi, Wenyi Kang

“…Western blotting experiments showed that compounds 2 and 4 up-regulated the protein expressions of p-IRS, PI-3K, p-AKT and glucose transporter 4 (GLUT4), and promoted the transcription of GLUT4 mRNA. Therefore, the mechanisms of compounds 2 and 4 were presumed to improve IR by activating PI-3K/AKT signaling pathway.…”
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Fecal microbiome change in patients with ulcerative colitis after fecal microbiota transplantation by A. Y. Tikunov, V. V. Morozov, A. N. Shvalov, A. V. Bardasheva, E. V. Shrayner, O. A. Maksimova, I. O. Voloshina, V. V. Morozova, V. V. Vlasov, N. V. Tikunova

“…The main method is a comparative analysis of 16S ribosomal RNA sequence libraries constructed using fecal samples obtained from patients with ulcerative colitis before and after FT and sequenced on the Illumina MiSeq platform. …”
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Spatial and temporal variation of Antarctic microbial interactions: a study around the west Antarctic Peninsula by Swan L. S. Sow, Willem H. van de Poll, Rachel Eveleth, Jeremy J. Rich, Hugh W. Ducklow, Patrick D. Rozema, Catherine M. Luria, Henk Bolhuis, Michael P. Meredith, Linda A. Amaral-Zettler, Julia C. Engelmann

“…Results We report on the seasonal diversity, taxonomic overview, as well as predicted inter-and intra-domain causal effects (interactions) of the bacterial and microbial eukaryotic communities close to the Palmer station and at the Rothera time-series site between July 2013 and April 2014. Our 16S- and 18S-rRNA gene amplicon sequencing data showed that across all seasons, both bacteria and microbial eukaryotic communities were considerably different between the two sites which could be attributed to seawater temperature, and sea ice coverage in combination with sea ice type differences. …”
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Assembly and comparative analysis of the complete mitochondrial genome of red raspberry (Rubus idaeus L.) revealing repeat-mediated recombination and gene transfer by Huajie Zhang, Minghui Yan, Lijuan Li, Zhuo Jiang, Ye Xiong, Yusheng Wang, Caleb Onoja Akogwu, Olutayo Mary Tolulope, Hao Zhou, Yanxia Sun, Hengchang Wang

“…Furthermore, 52 homologous sequences between the mitogenome and plastome were identified, including six complete protein-coding genes and 12 tRNA genes. We also detected 828 homologous fragments between the nuclear genome and mitogenome, including one trnM-CAU gene. …”
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High interstitial fluid pressure enhances USP1-dependent KIF11 protein stability to promote hepatocellular carcinoma progression by Zhengyi Wu, Chao Li, Shouhua Zhang, Liang Sun, Junwen Hu, Bingbing Qiu, Shuiqiu Liu, Yiran Hong, Tianxiang Chen, Kai Wang, Xiangbao Yin, Jinlong Yan

“…Omics analysis and subsequent molecular validation revealed that KIF11 protein levels were markedly upregulated under HIFP, despite no significant change in mRNA levels. Further investigation showed that this upregulation was linked to a reduction in KIF11's ubiquitin-mediated degradation, suggesting that the HIFP environment stabilizes KIF11 expression by inhibiting its degradation pathway. …”
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A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans by Fruzsina Kovács, Ágnes Jakab, Noémi Balla, Zoltán Tóth, Dávid Balázsi, Lajos Forgács, Andrea Harmath, Aliz Bozó, Ágota Ragyák, László Majoros, Renátó Kovács

“…Moreover, HSL reduced intracellular iron and zinc levels (p < 0.05-0.001); furthermore, it modulated C. auris metabolism toward beta-oxidation, which may be associated with the observed reduction in in vivo virulence at lower HSL concentrations compared with C. albicans. RNA-sequencing transcriptome analysis of C. auris revealed 67 and 306 upregulated genes, as well as 111 and 168 downregulated genes, in response to 100 and 200-μM HSL, respectively. …”
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RETRACTED ARTICLE: A prospective diagnostic model for breast cancer utilizing machine learning to examine the molecular immune infiltrate in HSPB6 by Lizhe Wang, Yu Wang, Yueyang Li, Li Zhou, Sihan Liu, Yongyi Cao, Yuzhi Li, Shenting Liu, Jiahui Du, Jin Wang, Ting Zhu

“…A temporal analysis of breast cancer cells using single-cell RNA sequencing provided insights into cellular developmental trajectories and highlighted changes in expression patterns across key genes during disease progression. …”
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Taohong Siwu Decoction Exerts a Beneficial Effect on Cardiac Function by Possibly Improving the Microenvironment and Decreasing Mitochondrial Fission after Myocardial Infarction by Zhi-rong Luo, Han Li, Zhong-xin Xiao, Shui-jin Shao, Tian-tian Zhao, Yue Zhao, Fang-fang Mou, Bo Yu, Hai-dong Guo

“…The infarct size and collagen deposition in the infarct area were measured using Masson’s trichrome staining, and the number of CD31- and α-SMA-positive blood vessels in the peri-infarct and infarct area was evaluated by immunofluorescent staining. The mRNA expression of bFGF, IGF-1, and HGF was detected using RT-PCR assay. …”
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Half-Elemental Diet Shifts the Human Intestinal Bacterial Compositions and Metabolites: A Pilot Study with Healthy Individuals by Jun Miyoshi, Daisuke Saito, Mio Nakamura, Miki Miura, Tatsuya Mitsui, Toru Kudo, Shinnosuke Murakami, Minoru Matsuura, Tadakazu Hisamatsu

“…Fecal DNA and metabolites were extracted from the samples. We performed 16S rRNA gene amplicon sequencing and metabolomic analysis to examine the bacterial compositions and intestinal metabolites. …”
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Protective effect of adiponectin on oxidative stress-induced ovarian granulosa cell senescence in geese by Yan Zheng, Yunqiao Qiu, Ming Gao, Qianhui Wang, Lei Yu, Zhongzan Cao, Xinhong Luan

“…The results showed that ADPN could alleviate oxidative stress and cell cycle arrest in GCs, reduce the expression of the senescence-associated secretory phenotype (SASP)-related genes IL-6 and IL-8, regulate the metabolic capacity of GCs, reduce the accumulation of SA-β-gal, maintain telomere length, and alleviate the senescence of GCs induced by D-gal. The RNA-seq results provided further evidence for the regulatory effect of ADPN on GC senescence. …”
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Effects of lipid levels on growth performance and glucose and lipid metabolism of adult Nile tilapia (Oreochromis niloticus) fed with high carbohydrate diets by Ningning Xie, Jianmin Zhang, Ming Jiang, Xiaolin Meng, Lixue Dong, Xing Lu, Hua Wen, Juan Tian

“…Tilapia also showed an adaption to D1 and D2 diets with increased digestive enzyme activities and enhanced mRNA expression levels of glucose carriers. Furthermore, D1 and D2 diets influenced the glucose and lipid metabolism in muscle. …”
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