Integration of metabolomic and transcriptomic analyses reveals regulatory functions of the ChREBP transcription factor in energy metabolism by Jie An, Inna Astapova, Guofang Zhang, Andrew L. Cangelosi, Olga Ilkayeva, Hannah Marchuk, Michael J. Muehlbauer, Tabitha George, Joseph Brozinick, Mark A. Herman, Christopher B. Newgard

“…Integrated transcriptomic and metabolomic analyses in rats with GalNac-siRNA-mediated suppression of ChREBP expression in liver reveal other ChREBP functions. …”
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Genetic profiling of multidrug-resistant Acinetobacter baumannii from a tertiary care center in Malaysia by Aisyah Syakirah Shahari, Navindra Kumari Palanisamy, Fadzilah Mohd Nor

“…The isolates were identified by Vitek 2 and PCR amplification of the 16S rRNA gene followed by sequencing. The isolates were tested against imipenem, ceftazidime, amikacin, gentamicin, ampicillin, and ciprofloxacin using disk diffusion, Epsilometer test, and broth microdilution. …”
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Characterization of the complete genome sequence of the recombinant norovirus GII.P16/GII.4_Sydney_2012 revealed in Russia by E. V. Zhirakovskaia, A. Y. Tikunov, S. N. Sokolov, B. I. Kravchuk, E. I. Krasnova, N. V. Tikunova

“…These small non-envelope viruses with a single-stranded (+)RNA genome are characterized by high genetic variability. …”
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In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging by Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn

“…MSCs were transduced with retroviruses to generate CXCR4-overexpressing MSCs (MSC-CXCR4/Fluc2) and MSCs (MSC/Fluc2). CXCR4 mRNA and protein expression was confirmed by RT-PCR and Western blotting, respectively, and it was higher in MSC-CXCR4/Fluc2 than in naive MSCs. eGFP expression was confirmed by confocal microscopy. …”
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Clinical significance and pro-oncogenic function of DBF4 in clear cell renal cell carcinoma by Liuyan Chen, Lvying Wu, Minying Tang, Yuanhang Cheng, Kuanyin Wang, Jianan Zhang, Wenyi Deng, Lingfeng Zhu, Jin Chen

“…Various assays, including assessments of cell proliferation, apoptosis, the cell cycle, cell migration and invasion, and colony formation, and xenograft tumor models were subsequently performed following to the knockdown of DBF4 expression via shRNA. Results Bioinformatics analyses revealed that DBF4 is significantly overexpressed in ccRCC tissues compared with adjacent normal tissues. …”
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The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus by Aslıhan Şeyda DOĞAN, Mukaddes PALA, Şenay GÖRÜCÜ YILMAZ, Ayfer BECEREN, Aydın KARABULUT, Yalçın POLAT, Hatice Kübra ELÇİOĞLU

“…Results: As a result of the validation, it was determined that three miRNAs (rno-miR-15b-5p, rno-miR-196b-3p, and rno-miR-208a-5p) were downregulated, one miRNA (rno-miR-200c-3p) was upregulated (p<0.05). …”
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Mechanism of Mongolian Medicine Batri-7 on Salmonella Enteritis by Yang D, Chen S, Borijihan H, Aoqier A, Sarula S, Siqin S, Manda M, Temuqile T, Baigude H

“…Simultaneously, the Minimum Inhibitory Concentration of BT-7 was evaluated against bacterial by microbroth dilution method, BT-7 regulation of Salmonella typhimurium gene was performed by RNA-Seq methods and verified by qRT-PCR.Results: In the LC-MS/MS assay, negative and positive-ion modes are identified for 511 and 699 compounds from BT-7, respectively. …”
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Epidermal stem cell derived exosomes-induced dedifferentiation of myofibroblasts inhibits scarring via the miR-203a-3p/PIK3CA axis by Shixin Zhao, Haoran Kong, Dahu Qi, Yushuang Qiao, Yu Li, Zhiming Cao, Hanwen Wang, Xuefeng He, Hengdeng Liu, Hao Yang, Suyue Gao, Tao Liu, Julin Xie

“…Epidermal stem cell-derived extracellular vesicles (EpiSC-EVs) were isolated via ultracentrifugation and filtration, followed by miRNA sequencing to identify miRNAs targeting key molecules. …”
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Rare Taxa Are Key Links in Regional Antimicrobial Resistance Profiles in Dusts Across Diverse North American Regions by Paul B. L. George, Florent Rossi, Marc Veillette, Amélia Bélanger Cayouette, Samantha Leclerc, Cindy Dumais, Nathalie Turgeon, Caroline Duchaine

“…Bacterial biomass was assessed using standard qPCR methods of the 16S rRNA gene, which was also used to assess bacterial biodiversity via metabarcoding. …”
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Strawberry dietary intervention influences diversity and increases abundances of SCFA-producing bacteria in healthy elderly people by Franziska Meiners, Bernd Kreikemeyer, Patrick Newels, Ingmar Zude, Michael Walter, Alexander Hartmann, Daniel Palmer, Georg Fuellen, Israel Barrantes

“…Participants in five groups consumed varying amounts of strawberries, freeze-dried strawberries, and capers in olive oil over 10 weeks as part of a randomized controlled trial. 16S rRNA sequencing was used to analyze differences in microbial composition, diversity, phenotypes, differential abundance, and functional pathways. …”
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Extracellular Vesicles from Amnion-Derived Mesenchymal Stem Cells Ameliorate Hepatic Inflammation and Fibrosis in Rats by Masatsugu Ohara, Shunsuke Ohnishi, Hidetaka Hosono, Koji Yamamoto, Kohei Yuyama, Hideki Nakamura, Qingjie Fu, Osamu Maehara, Goki Suda, Naoya Sakamoto

“…AMSC-EVs significantly decreased the number of Kupffer cells (KCs) in the liver of rats with NASH and the mRNA expression levels of inflammatory cytokines such as tumor necrosis factor- (Tnf-) α, interleukin- (Il-) 1β and Il-6, and transforming growth factor- (Tgf-) β. …”
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The injury effect of osteopontin in sepsis-associated lung injury by Qian Wang, Zhicai Yu, Zhixin Song, Xue Lu, Zhu Li, Dandan Pi, Jing Li, Feng Xu

“…Mechanistic investigations in MH-s cells revealed that OPN-siRNA suppressed the LPS-induced macrophage inflammatory response by inhibiting caspase1-dependent classical pyroptosis signaling pathway. …”
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Cyclophilin–CD147 interaction enables SARS-CoV-2 infection of human monocytes and their activation via Toll-like receptors 7 and 8 by Gabor Tajti, Laura Gebetsberger, Gregor Pamlitschka, Katharina Aigner-Radakovics, Judith Leitner, Peter Steinberger, Hannes Stockinger, Anna Ohradanova-Repic

“…Secondly, upon viral uptake via the cyclophilin-CD147 interaction, that can be inhibited by specific CD147 blocking antibodies or competition with recombinant human cyclophilin A and B, SARS-CoV-2 RNA is recognized by TLR7/8 in endosomes, leading to upregulation of tumor necrosis factor (TNF), interleukin (IL)-1β and IL-6, comprising the core hyperinflammatory signature. …”
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Immune regulatory genes impact the hot/cold tumor microenvironment, affecting cancer treatment and patient outcomes by Mengmeng Sang, Jia Ge, Juan Ge, Juan Ge, Gu Tang, Qiwen Wang, Jiarun Wu, Liming Mao, Liming Mao, Xiaoling Ding, Xiaorong Zhou

“…Additionally, hub genes that regulate the tumor microenvironment were identified, and their expression patterns were analyzed using single-cell RNA sequencing. Furthermore, drug sensitivity and molecular docking analyses were performed to identify potential drug candidates capable of transforming cold tumors into hot tumors. …”
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Characterization of mammary glands and milk fat globule transcripts in lactating buffalo and goats by Hancai Jiang, Xiaoxian Xu, Shuwan Wang, Xinhui Song, Ling Li, Qingyou Liu, Kuiqing Cui, Deshun Shi, Jian Wang, Hui Li, Jieping Huang, Zhipeng Li

“…Here, we aimed to reveal the universal rule of the transcript profiles of the milk fat globules and mammary glands from buffaloes and goats by RNA-sequencing analysis. Results showed that, in buffalo, 97 % of mRNAs were expressed in both milk fat globules and mammary glands, with 45 % showing differential expression. …”
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Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples by M. F. Kearney, K. Lee, R. K. Bagni, A. Wiegand, J. Spindler, F. Maldarelli, P. A. Pinto, W. M. Linehan, C. D. Vocke, K. A. Delviks-Frankenberry, R. W. deVere White, G. Q. Del Prete, J. W. Mellors, J. D. Lifson, V. N. KewalRamani, V. K. Pathak, J. M. Coffin, S. F. J. Le Grice

“…Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. …”
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In silico analysis of hub genes and regulatory networks implicates the putamen in non-motor Parkinson’s disease disorders by Stephen Tunmise Akanbi, Joshua Ayodele Yusuf, Thompson Oluwaferanmi Ayandele, Dayo Samson Oladipupo, Darasimi Racheal Olorunlowu, Blessing Dorcas Olawuyi, Joshua Oluwafisayo Adepoju, Dolapo Emmanuel Opasina, Elizabeth Kehinde Opoola, Uchenna Victor Ugwuanyi, Olufunto Omodele Adeleye

“…Also, we constructed transcription factor (TF)-hub gene expression networks, miRNA-hub gene expression networks, and disease hub gene association networks using the JASPAR database, Tarbase database, and DisGeNET via NetworkAnalyst platform, respectively. …”
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Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China by Yuxin Li, Yinan Wang, Xin Gao, Lihong Luo, Bohan Zhang, Xiao Wang, Jing Li, Ruijia Wu, Lixin He, Wenxuan Li, Changwei Qiu

“…The expression of two aminoglycoside modifying enzyme genes, <i>aacA-aphD</i> and <i>aac (6′)-Ib</i>, and three 16S rRNA methyltransferase genes, <i>rmtB</i>, <i>rmtE</i> and <i>npmA</i>, were analyzed by PCR. …”
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Drosophila ubiquitin-specific peptidase 14 stabilizes the PERIOD protein by regulating a ubiquitin ligase SLIMB by So Who Kang, Jung-Eun Park, Soonhyuck Ok, Minhui Um, Hyeonjeong Son, Seunghee Byun, Nayoung Park, Su Jin Lee, Thị Xuân Thùy Trần, Gyeongmin Kim, Jeonghun Yeom, Kyunggon Kim, Eun Young Kim, Min-Ji Kang

“…We find that overexpression of USP14 in clock cells reduces PER protein levels without altering its mRNA levels whereas USP14 knockdown increases PER protein levels, suggesting that USP14 regulates PER post-translationally. …”
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Antiviral Activity and Underlying Mechanism of <i>Moslae herba</i> Aqueous Extract for Treating SARS-CoV-2 by Yan Feng, Qiong Ge, Jian Gao, Zhuoying Wu, Yunyi Zhang, Haiyan Mao, Beibei Wu, Changping Xu

“…Of the nine, the PNP and TPI1 were identified as core targets as AEMH treatment significantly regulated the mRNA expression level of the two genes on infected cells. …”
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