Undernutrition disrupts jejunal and ileal microbiota and epithelial tissue homeostasis in a pregnant sheep model by Weibin Wu, Muhammad Faheem Akhtar, Jiahong Geng, Huizhen Lu, Muhammad Ajwad Rahim, Jianbo Cheng, Xiaoling Ding, Shengyong Mao, Yanfeng Xue

“…After 15-d treatment, all ewes were slaughtered, and jejunal and ileal digesta and epithelium samples were collected for 16S rRNA gene sequencing and transcriptome sequencing, respectively. …”
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Thermosensitive injectable in situ forming hydrogel incorporating anti-oxidative stress and anti-inflammatory nanoparticles for vital pulp therapy of pulpitis by Lixin Zhang, Xianling Ning, Jingyi Cui, Yanqiang Zhao, Fei Xie, Ningxin Zhu, Dan Wang, Man Qin, Zhiqiang Lin, Yuanyuan Wang

“…As a direct pulp capping material, PBNPs@PPP suppressed inflammation progression in pulpitis in rats while also reducing cell necrosis and inflammatory cell infiltration in pulp tissue. RNA sequencing analyses showed that the functions of PBNPs@PPP were highly involved in anti-inflammatory pathways. …”
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RIPK2-Mediated Autophagy and Negatively Regulated ROS-NLRP3 Inflammasome Signaling in GMCs Stimulated with High Glucose by Chenlin Gao, Jiao Chen, Fang Fan, Yang Long, Shi Tang, Chunxia Jiang, Jiying Wang, Youhua Xu, Yong Xu

“…GMCs were divided into the following groups: normal group (NC), high glucose group (HG), and RIPK2 siRNA group. RIPK2, LC3, caspase1, and IL-1β levels were measured by western blotting and RT-PCR. …”
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Survey of the Applications of NGS to Whole-Genome Sequencing and Expression Profiling by Jong-Sung Lim, Beom-Soon Choi, Jeong-Soo Lee, Chanseok Shin, Tae-Jin Yang, Jae-Sung Rhee, Jae-Seong Lee, Ik-Young Choi

“…Whole-genome expression profile data are useful to approach genome system biology with quantification of expressed RNAs from a whole-genome transcriptome, depending on the tissue samples. The hybrid mRNA sequences from Rohce/454 and Illumina/Solexa are more powerful to find novel genes through de novo assembly in any whole-genome sequenced species. …”
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Development and Characterization of Anti-Nitr9 Antibodies by Radhika N. Shah, Ivan Rodriguez-Nunez, Donna D. Eason, Robert N. Haire, Julien Y. Bertrand, Valērie Wittamer, David Traver, Shila K. Nordone, Gary W. Litman, Jeffrey A. Yoder

“…Within the zebrafish NITR family, nitr9 is the only gene predicted to encode an activating receptor. However, alternative RNA splicing generates three distinct nitr9 transcripts, each of which encodes a different isoform. …”
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ERK1/2 Inhibition Alleviates Diabetic Cardiomyopathy by Suppressing Fatty Acid Metabolism by Erin McLean, Caroline De Roo, Annabel Maag, Megan Coble, Jefferson Cano, Ruijie Liu

“…Real-time polymerase chain reaction (PCR) analysis of the messenger ribonucleic acid (mRNA) expression was performed for hypertrophy (ANF, BNP, and βMHC), fibrosis (Col3α1), and fatty acid metabolism genes (PPARα, CPT1A, and FACS). …”
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PLIN5 contributes to lipophagy of hepatic stellate cells induced by inorganic arsenic by Haomiao Li, Xuri Xie, Tianming Qiu, Jingyuan Zhang, Jie Bai, Guang Yang, Ningning Wang, Xiaofeng Yao, Xiance Sun

“…Remarkably, arsenic exposure increased the expression levels of Perilipin 5 (PLIN5), a lipid droplet-associated protein, both at the mRNA and protein levels. Moreover, silencing PLIN5 influenced arsenic-induced lipolysis. …”
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Calcitonin Gene-Related Peptide (Cgrp), Adrenomedullin (Am), Amylin, And Calcitonin (Ct) Receptors And Overlapping Biological Actions by J.A. Fischer, W. Born, R. Muff

“…Another CGRP receptor isotype remains to be discovered in the cerebellum with no detectable CRLR encoding mRNA. Overlapping biological actions include inhibition of bone resorption obtained predominantly with CT, but also at high concentrations with CGRP, AM, and amylin. …”
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Enhanced membrane protein production in HEK293T cells via ATF4 gene knockout: A CRISPR-Cas9 mediated approach by Byung-Jo Choi, Ba Reum Kim, Ho Joong Choi, Ok-Hee Kim, Say-June Kim

“…The ATF4 gene was edited by infecting HEK293T cells with a lentivirus carrying optimized single-guide RNA (ATF4-KO-3) and Cas9 genes. Comparative evaluations were conducted using all-in-one and two-vector systems. …”
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Mechanism of Action of Decitabine in the Treatment of Acute Myeloid Leukemia by Regulating LINC00599 by Fan Du, Ting Jin, Li Wang

“…Reverse transcription polymerase chain reaction (RT-PCR) was performed to examine the expression of lncRNA LINC00599. The expression of apoptosis-related proteins was analyzed using western blotting. …”
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BRCA1 is involved in sustaining rapid antler growth possibly via balancing of the p53/endoplasmic reticulum stress signaling pathway by Qianqian Guo, Zhen Wang, Jiping Li, Chao Ma, Junjun Zheng, Hengxing Ba, Guokun Zhang, Chunyi Li

“…Lack of BRCA1 promoted cell apoptosis and cell senescence and inhibited cell proliferation and cell self-renewal. RNA-seq results showed that in the absence of BRCA1, the p53 signaling pathway was significantly up-regulated. …”
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Antimicrobial Susceptibility of <i>Glaesserella parasuis</i> to Macrolides and Characterization of <i>erm</i>(T)-Carrying Mobile Elements on Chromosome by Peng Zhang, Changmin Li, Shuna Shang, Ting Huang, Junqi Liu, Qianwen Ge, Xiaoping Liao, Liangxing Fang, Yang Yu

“…The macrolide resistance gene <i>erm</i>(T) and the A2059G mutation in 23S rRNA were detected in the current study. To the best of our knowledge, integrative and conjugative element (ICE)-borne <i>erm</i>(T) in <i>G. parasuis</i> is reported for the first time in this study. …”
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Adjunctive diagnostic tool for histopathological classification of congenital mesoblastic nephroma based in molecular genetic findings by Hiroshi Hamada, Kenichi Kohashi, Takeshi Iwasaki, Mikiko Hashisako, Yuko Hino, Masahiro Fukuhara, Amane Kamouchi, Naonori Kawakubo, Tatsuro Tajiri, Yoshinao Oda

“…Reverse transcription-polymerase chain reaction was performed for the NTRK gene fusion and EGFR-ITDs in all cases. Comprehensive mRNA analysis was performed using the nCounter® Gene Expression Assay. …”
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Improving polyketide biosynthesis by rescuing the translation of truncated mRNAs into functional polyketide synthase subunits by Yan Liu, Chaoyi Song, Qingwen Cui, Hongluan Sun, Chanjuan Jiang, Ruofei Guo, Ruoting He, Zhen Li, Ji Luan, Hailong Wang

“…Splitting the large busA gene rescues translation of truncated mRNAs into functional PKS subunits, and increases the biosynthetic efficiency of butenyl-spinosyn PKS by 13-fold. The truncated mRNA translation rescue strategy will facilitate engineering of multi-domain proteins to enhance their functions.…”
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Sleep-Aiding Effect of Casein-Derived Peptide TPTLN and Its Underlying Mechanism by DENG Shijie, LIU Ling, HUANG Ju, FENG Fengqin, ZHAO Minjie, DU Juan

“…Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of 5-hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA), 5-hydroxyindoleacetic acid (5-HIAA), glutamate (Glu), β-endorphin (β-EP), cortisol, norepinephrine (NE) in the serum and brain tissue, and the GABA/Glu ratio was evaluated. real-time quantitative polymerase chain reaction (qPCR) was used to detect the effect of TPTLN on the mRNA relative expression levels of γ-aminobutyric acid A receptor α1 (GABAA-α1) and 5-hydroxytryptamine receptor 1A (5-HT1A) in the hypothalamus. …”
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A New Prognostic Risk Model Based on PPAR Pathway-Related Genes in Kidney Renal Clear Cell Carcinoma by Yingkun Xu, Xiunan Li, Yuqing Han, Zilong Wang, Chenglin Han, Ningke Ruan, Jianyi Li, Xiao Yu, Qinghua Xia, Guangzhen Wu

“…For this study, we first found the PPAR pathway-related genes on the gene set enrichment analysis (GSEA) website and found the KIRC mRNA expression data and clinical data through TCGA database. …”
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Genetically edited human placental organoids cast new light on the role of ACE2 by Anya L. Arthurs, Bianca Dietrich, Martin Knöfler, Caleb J. Lushington, Paul Q. Thomas, Fatwa Adikusuma, Jessica M. Williamson, Susan Babikha, Tyla Damhuis, Tanja Jankovic-Karasoulos, Melanie D. Smith, Kirsty G. Pringle, Claire T. Roberts

“…Placental organoids with the ACE2 rs2074192 TT genotype had significantly higher expression of ACE2 mRNA and ACE2 protein with elevated ACE2:ACE expression ratio and no change in ACE protein. …”
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Exploring the influence of pre-analytical variables on gene expression measurements and relative expression orderings in cancer research by Tian Tian, Guie Lai, Ming He, Xiaofang Liu, Yun Luo, You Guo, Guini Hong, Hongdong Li, Kai Song, Hao Cai

“…These paired samples were utilized to assess the impact of pre-analytical variables on gene expression measurements and REOs, including sampling methods, tumor sample heterogeneity, fixed time delays, preservation conditions, degradation levels, library preparation kits, amplification kits, RNA quantity, measuring platforms, and laboratory sites at single and multi-variable level. …”
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Chromosome-level reference genome assembly for the mountain hare (Lepus timidus) by Fekete, Zsófia, Absolon, Dominic E., Michell, Craig, Wood, Jonathan M. D., Goffart, Steffi, Pohjoismäki, Jaakko L. O.

“…We present here a high-quality genome assembly of a male mountain hare (Lepus timidus Linnaeus), from Ilomantsi, Eastern Finland, utilizing an isolated fibroblast cell line as the source for high quality DNA and RNA. Following the previously published brown hare reference genome assembly, the mountain hare is the second Finnish pilot species for the European Reference Genome Atlas (ERGA) initiative, a collaborative effort to generate reference genomes for European biodiversity. …”
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Splicing and expression dynamics of SR genes in hot pepper (Capsicum annuum): regulatory diversity and conservation under stress by Lin Li, Yueqin Zhang, Rui Zhang, Rui Zhang, Xiangtao Cen, Yongxiang Huang, Yongxiang Huang, Hanqiao Hu, Hanqiao Hu, Xingyu Jiang, Xingyu Jiang, Yu Ling, Yu Ling

“…Interestingly, a cross-species comparative study with Arabidopsis (Arabidopsis thaliana) and tomato (Solanum lycopersicum) showed that many AS events impact the region which codes the RNA recognition motif (RRM) domain of the protein, indicating a conserved regulatory mechanism of SR proteins in plants. …”
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