Engineered CAF-cancer cell hybrid membrane biomimetic dual-targeted integrated platform for multi-dimensional treatment of ovarian cancer by Yuwei Yao, Jiarui Zhang, Kexin Huang, Yingying Peng, Shuangshuang Cheng, Shuangge Liu, Ting Zhou, Jinhua Chen, Haojia Li, Yingchao Zhao, Hongbo Wang

“…Result We constructed PH20-overexpressing cancer-associated fibroblast (CAF)-cancer hybrid-cell membrane vesicles (PH20/CCM) for the dual-targeted delivery of carboplatin (CBP) and siRNA targeting p65 (sip65) loaded on the poly (dimethyl diallyl ammonium chloride) (PDDA)-modified MXene (PMXene), named PMXene@CBP-sip65 (PMCS). …”
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Expression Profiling and Functional Implications of a Set of Zinc Finger Proteins, ZNF423, ZNF470, ZNF521, and ZNF780B, in Primary Osteoarthritic Articular Chondrocytes by Maria Mesuraca, Olimpio Galasso, Leonardo Guido, Emanuela Chiarella, Stefania Scicchitano, Renaud Vatrinet, Giovanni Morrone, Heather M. Bond, Giorgio Gasparini

“…Interestingly, this decrease was mirrored by the similarly reduced expression of PPARγ, a known target of ZNF423 with anti-inflammatory and chondroprotective properties. The ZNF521 mRNA instead was abundant in all primary chondrocytes studied; the RNAi-mediated silencing of this gene significantly altered the COL2A/COL1 expression ratio, associated with the maintenance of the differentiated phenotype, in chondrocytes cultivated in alginate beads. …”
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Unveiling early-life microbial colonization profile through characterizing low-biomass maternal-infant microbiomes by 2bRAD-M by Shuwen Hou, Yuesong Jiang, Feng Zhang, Tianfan Cheng, Dan Zhao, Jilong Yao, Ping Wen, Ping Wen, Lijian Jin, Shi Huang

“…We collected breast milk samples, maternal feces, and infant meconium, comparing the results from 2bRAD-M with those from both commonly used 16S rRNA amplicon sequencing and the gold-standard whole metagenomics sequencing (WMS).ResultsThe accuracy and robustness of 2bRAD-M were demonstrated through its consistently high correlation of microbial individual abundance and low whole-community-level distance with the paired WMS samples. …”
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Microbiome of <i>Hyalomma dromedarii</i> (Ixodida: Ixodidae) Ticks: Variation in Community Structure with Regard to Sex and Host Habitat by Nighat Perveen, Alejandro Cabezas-Cruz, Daniil Iliashevich, Lianet Abuin-Denis, Olivier Andre Sparagano, Arve Lee Willingham

“…To better understand the tick microbiome influenced by sex and host habitat, we analyzed the bacterial community of <i>H. dromedarii</i> male and female ticks collected from camel farms, livestock markets, and slaughterhouses, representing the range of major habitats in the UAE, by sequencing the 16S rRNA gene. Tick samples were collected during 2022 and 2023. …”
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Dietary Supplementation with Inulin Modulates the Gut Microbiota and Improves Insulin Sensitivity in Prediabetes by Xiaojing Wang, Tong Wang, Qian Zhang, Li Xu, Xinhua Xiao

“…Intestinal microbiota profile was evaluated using the Illumina MiSeq platform based on V3-V4 bacterial 16S rRNA gene. Results. The mean age of 49 subjects was 56.6 ± 6.9 years and BMI was 25.07 ± 3.02 kg/m2. …”
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Analysing DNA methylation and transcriptomic signatures to predict prostate cancer recurrence risk by Fahad M. Aldakheel, Hadeel Alnajran, Shatha A. Alduraywish, Ayesha Mateen, Mohammed S. Alqahtani, Rabbani Syed

“…This study employs a machine learning approach to identify DNA methylation and RNA expression biomarkers predictive of PCa recurrence using datasets from The Cancer Genome Atlas (TCGA). …”
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Topical neurokinin-1 receptor antagonism ameliorates ocular pain and prevents corneal nerve degeneration in an animal model of dry eye disease by Amirreza Naderi, Yukako Taketani, Shudan Wang, Francesca Kahale, Ann Yung, Pier Luigi Surico, Yihe Chen, Reza Dana

“…Trigeminal ganglions (TG) were collected to determine SP levels by ELISA and transient receptor potential cation channel subfamily V member 1 (TRPV1), transient receptor potential cation channel subfamily M (melastatin) member 8, c-Fos, and activating transcription factor 3 (ATF3) mRNA levels by real-time polymerase chain reaction. …”
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Development of a Cell-Based High-Throughput Screening Assay to Identify Porcine Host Defense Peptide-Inducing Compounds by Zhuo Deng, Jing Wang, Wentao Lyu, Xuwen Wieneke, Robert Matts, Xi Ma, Guolong Zhang

“…Eight compounds were subsequently observed to be comparable to sodium butyrate in inducing PBD3 mRNA expression in parental IPEC-J2 cells in the low micromolar range. …”
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Circ_0001084/miR-181c-5p/PTPN4 Axis Mitigates Cardiomyocyte Injury by Modulating the TLR4/NF-&kappa;B Pathway: Insights into Therapeutic Potential for Myocardial Reperfusion Injury by Deng H, Fan Q, Huang L, Ouyang W, Zhu W

“…This suggests a novel therapeutic pathway for myocardial I/R injury treatment, highlighting the interplay between non-coding RNAs and cellular stress responses.Conclusion: circ_0001084 acts as a competing endogenous RNA for miR-181c-5p, enhancing PTPN4 expression and inhibiting the TLR4/NF-κB signaling pathway. …”
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Effects of аntimicrobials on <i>Pseudomonas aeruginosa</i> biofilm formation by U. M. Nemchenko, K. O. Sitnikova, N. L. Belkova, E. V. Grigorova, N. M. Voropaeva, M. V. Sukhоreva, E. S. Sukhareva, E. D. Savilov

“…., Czech Republic), and confirmed by mass spectrometric analysis and 16S rRNA gene sequencing. Antimicrobial activity was assessed by the degree of inhibition of cell growth in planktonic and biofilm forms (on a flat-bottomed 96-well plastic immunological plate). …”
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MRTX1133 attenuates KRASG12D mutated-colorectal cancer progression through activating ferroptosis activity via METTL14/LINC02159/FOXC2 axis by Junwei Zou, Xiuhua Shi, Zhaoying Wu, Siyuan Zuo, Xiaolei Tang, Hailang Zhou, Yong Huang

“…Therefore, this study was designed to identify a novel lncRNA involved in KRASG12D-mutated CRC and to elucidate its molecular mechanisms. …”
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Evaluation of IgG binding capability to SARS-CoV-2 variants in early COVID-19 convalescent sera using an indirect ELISA by Behzad Hussain, Peizhe Zhao, Yu Liu, Bo Yang, Xiaoxiong Li, Zhichao Zhang, Guoqiang Feng, Demei Zhang, Defen Lu, Wu Changxin

“…Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single-stranded RNA virus which has resulted in the Coronavirus Disease 2019 (COVID-19) pandemic and has infected millions of people all over the world. …”
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Advancing the Indian cattle pangenome: characterizing non-reference sequences in Bos indicus by Sarwar Azam, Abhisek Sahu, Naveen Kumar Pandey, Mahesh Neupane, Curtis P Van Tassell, Benjamin D Rosen, Ravi Kumar Gandham, Subha Narayan Rath, Subeer S Majumdar

“…Furthermore, 926 BICIs were identified within 682 protein-coding genes, 54 long non-coding RNAs (lncRNA), and 18 pseudogenes. These protein-coding genes were enriched for functions such as chemical synaptic transmission, cell junction organization, cell-cell adhesion, and cell morphogenesis. …”
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Oral Supplementation with Three Vegetable Oils Differing in Fatty Acid Composition Alleviates High-Fat Diet-Induced Obesity in Mice by Regulating Inflammation and Lipid Metabolism by Waleed Aldamarany, Huang Taocui, Deng Liling, Yang Wanfu, Geng Zhong

“…It also decreased leptin, pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and (IL)-1beta (IL-1β), and increased anti-inflammatory adipokine adiponectin at both secretion and mRNA expression levels in the epididymal adipose tissue (EAT). …”
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Epigenome-wide association study of Chinese monozygotic twins identifies DNA methylation loci associated with estimated glomerular filtration rate by Xueting Qi, Jingjing Wang, Tong Wang, Weijing Wang, Dongfeng Zhang

“…Gene expression profiling sequencing was employed to measure mRNA expression levels, and the GEE model was used to investigate the association between gene expression and eGFR. …”
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Photodynamic Therapy with Protoporphyrin IX Precursors Using Artificial Daylight Improves Skin Antisepsis for Orthopedic Surgeries by Tiziano A. Schweizer, Julia S. Würmli, Julia Prinz, Maximilian Wölfle, Roger Marti, Hendrik Koliwer-Brandl, Ashley M. Rooney, Vanni Benvenga, Adrian Egli, Laurence Imhof, Philipp P. Bosshard, Yvonne Achermann

“…In selected participants, 16S rRNA-based amplicon deep sequencing was performed. …”
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Comparison of glimepiride and linagliptin in the treatment of non-alcoholic hepatic disease with type 2 diabetes mellitus by Feng Tian, Yali Guo, Liping Zhou, Qunying Yao, Xiaoyan Liang, Jiaxin Lu, Aiping He, Jie Shen

“…Introduction Nonalcoholic fatty liver disease (NAFLD) with type 2 diabetes mellitus (T2DM) is associated with severe clinical outcomes. MicroRNA (miR)-210 has been reported to be related to T2DM and lipid metabolism. …”
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Paradoxical Glucose-Sensitizing yet Proinflammatory Effects of Acute ASP Administration in Mice by Alexandre Fisette, Pegah Poursharifi, Katerina Oikonomopoulou, Mercedes N. Munkonda, Marc Lapointe, Katherine Cianflone

“…ASP supplementation induced a proinflammatory phenotype, with higher levels of cytokines including IL-6 and TNF-α in plasma and in adipose tissue, liver, and skeletal muscle mRNA. Additionally, ASP increased M1 macrophage content of these tissues. …”
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Global meta‐analysis reveals the drivers of gut microbiome variation across vertebrates by Yong Xie, Songsong Xu, Yufei Xi, Zixin Li, Erwei Zuo, Kai Xing, Lijing Bai, Kui Li

“…We here collected 6508 samples from 113 vertebrate species covering diverse classes, feeding behaviors, and host habitats based on 16S rRNA gene sequencing. The results showed that host diet pattern had a significant impact on gut microbiome variation, which might drive taxonomic and functional contents of gut microbiome across vertebrates. …”
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Vitamin D Regulates the Expression of Glucocorticoid Receptors in Blood of Severe Asthmatic Patients by Bassam Mahboub, Saba Al Heialy, Mahmood Yaseen Hachim, Rakhee K. Ramakrishnan, Ashraf Alzaabi, Rania Medhat Seliem, Laila Ibraheem Salameh, Sameen Masooma Toor, Fathelrahman Salem Shendi, Ola Mohamed Al Ali, Basil Khalid Safarini, Wafa Taleb Erabia, Rabih Halwani, Qutayba Hamid

“…Upon VitD supplementation of severe asthmatic patients, there was a significant increase in the blood levels of GR-α with no change in GR-β mRNA expression. VitD supplementation also suppressed the blood levels of IL-17F and IL-4. …”
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