Identification of Potential Biomarkers and Immune Features of Sepsis Using Bioinformatics Analysis by Fang-Chen Gong, Ran Ji, Yu-Ming Wang, Zhi-Tao Yang, Ying Chen, En-Qiang Mao, Er-Zhen Chen

“…The three datasets included 156 whole blood RNA samples from 89 sepsis patients and 67 healthy controls. …”
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USP20 mediates malignant phenotypic changes in bladder cancer through direct interactions with YAP1 by Wensun Chen, Siqi Wu, Yifan Chen, Weijian Li, Yiqing Cao, Yingchun Liang, Xiyu Dai, Xinan Chen, Yilin Chen, Tian Chen, Shenghua Liu, Chen Yang, Haowen Jiang

“…In this study, we identified USP20 as a Hippo/YAP1 pathway-related deubiquitinase using combined siRNA screening and a deubiquitinase overexpression assay. …”
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Identification of Heat-Responsive Genes in Guar [Cyamopsis tetragonoloba (L.) Taub] by Aref Alshameri, Fahad Al-Qurainy, Abdel-Rhman Gaafar, Salim Khan, Mohammad Nadeem, Saleh Alansi

“…In the current study, the differential gene expression and the underlying metabolic pathways induced by heat treatment were investigated. An RNA-Seq study on Guar leaves was carried out to estimate gene abundance and identify genes involved in heat tolerance to better understand the response mechanisms to heat stress. …”
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Phytochemical Analysis and Anti-Inflammatory Activity of the Extracts of the African Medicinal Plant Ximenia caffra by Jing Zhen, Yue Guo, Tom Villani, Steve Carr, Thomas Brendler, Davis R. Mumbengegwi, Ah-Ng Tony Kong, James E. Simon, Qingli Wu

“…Cell-based assays show that the leaf extract inhibits the mRNA expression of proinflammatory genes (IL-6, iNOS, and TNF-α) by using RT-qPCR, implying its anti-inflammatory effects. …”
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Glutamine-glutamate centered metabolism as the potential therapeutic target against Japanese encephalitis virus-induced encephalitis by Mengyuan Li, Hang Yuan, Xiaofei Yang, Yingfeng Lei, Jianqi Lian

“…Exogenous addition of metabolites and associated compounds and RNA interference were employed to manipulate glutamine-glutamate metabolism to clarify its effects on viral replication. …”
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Pharmacological effects of fibroblast growth factor 21 are sex-specific in mice with the <em>lethal yellow</em> (A^y) mutation by E. N. Makarova, T. V. Yakovleva, N. Yu. Balyibina, K. O. Baranov, E. I. Denisova, A. D. Dubinina, N. A. Feofanova, N. M. Bazhan

“…FGF21 administration decreased hypothalamic POMC mRNA only in males. Thus, the pharmacological effect of FGF21 were significantly different in male and female Ay -mice; unlike males, females were resistant to catabolic effects of FGF21.…”
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miR-576-5p Promotes the Proliferation of Papillary Thyroid Carcinoma through the MAPK4-AKT Pathway by Rui Hai, Yang Zhou, Fan Li, Qingxi Guo, Yang Long, Fang Wang, Lian Cheng, Fei Wu, Shi Chen, Xiangyu Zhou

“…Background. MicroRNA-576-5p (miR-576-5p) plays an important role in different human cancers. …”
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Complete mitochondrial genome sequence analysis revealed double matrilineal components in Indian Ghoongroo pigs by Pranab Jyoti Das, Satish Kumar, Manasee Choudhury, Seema Rani Pegu, K. Meera, Rajib Deb, Sunil Kumar, Santanu Banik, Vivek Kumar Gupta

“…Furthermore, examination of tRNA genes and nucleotide composition of different genes namely rRNAs, COX1, COX2, ATP6, ND4, ND5, ND6, Cytb offered insights into genetic diversity within these pigs. …”
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Molecular Detection of Bartonella spp. in China and St. Kitts by Ke Huang, Patrick John Kelly, Jilei Zhang, Yi Yang, Weiguo Liu, Anwar Kalalah, Chengming Wang

“…By comparing highly conserved 16S rRNA sequences of the better-recognized Bartonella spp. on GenBank, we selected primers and probes for a genus-specific pan-Bartonella FRET-qPCR. …”
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A Five-Year Retrospective Study of Foot-and-Mouth Disease Outbreaks in Southern Africa, 2014 to 2018 by Elliot Mpolokang Fana, Sununguko Wata Mpoloka, Melvin Leteane, LaToya Seoke, Kelebogile Masoba, Mokganedi Mokopasetso, Aobakwe Rapharing, Tshephang Kabelo, Patricia Made, Joseph Hyera

“…Foot-and-mouth disease (FMD) virus (FMDv), like other ribonucleic acid (RNA) genome viruses, has a tendency to mutate rapidly. …”
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The comprehensive assessment of epigenetics changes during skin development by Li Lei, Ling Jiang, Yibo Hu, Menglu Chen, Jinhua Huang, Jing Chen, Qinghai Zeng

“…However, the minimal erythema dose of UV exposure altered multiple epigenetic effectors regulating extracellular matrix organization, cell-matrix adhesion, innate immune response, mitochondrial function and mRNA processing. In addition, epigenetic changes following UV exposure were more pronounced in the elderly skin compared to the younger skin. …”
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A gene expression atlas of Nicotiana tabacum across various tissues at transcript resolution by Shizhou Yu, Jufen Wan, Jufen Wan, Tenghang Xu, Tenghang Xu, Jie Zhang, Linggai Cao, Jie Liu, Hongfeng Liu, Xueliang Ren, Zhixiao Yang

“…In this study, we collected 599 tobacco RNA-seq datasets from 35 projects. 207,689 transcripts were identified in this study, of which 35,519 were annotated in the reference genome, while 172,170 transcripts were newly annotated. …”
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Chromatin environment-dependent effects of DOT1L on gene expression in male germ cells by Manon Coulée, Alberto de la Iglesia, Mélina Blanco, Clara Gobé, Clémentine Lapoujade, Côme Ialy-Radio, Lucia Alvarez-Gonzalez, Guillaume Meurice, Aurora Ruiz-Herrera, Pierre Fouchet, Julie Cocquet, Laïla El Khattabi

“…Here, by producing and integrating epigenomic and spike-in RNA-seq data, we decipher the molecular role of DOT1L during mouse spermatogenesis and show that it has opposite effects on gene expression depending on chromatin environment. …”
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Endometrial factors in the implantation failure spectrum: protocol of a MUltidisciplinary observational cohort study in women with Repeated Implantation failure and recurrent Misca... by Servaas Morré, Ron van Golde, Andrea Romano, Linda Stevens Brentjens, Denise Habets, Janneke Den Hartog, Salwan Al-Nasiry, Lotte Wieten, Aafke Van Montfoort

“…The study parameters are the steroid profile (steroid levels and mRNA levels, protein expression and activity of steroid enzymes) in endometrial tissue and peripheral blood, as well as the activating or inhibitory phenotype of uNK cells based on receptor expression in menstrual blood and endometrial tissue and determination of the vaginal and endometrial microbiome using the inter spacer bacterial profiling technique.Ethics and dissemination The protocol is approved by the local medical ethical review committee at the Maastricht University Medical Centre. …”
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Detection and Phylogenetic Characterization of Influenza D in Swedish Cattle by Ignacio Alvarez, Fereshteh Banihashem, Annie Persson, Emma Hurri, Hyeyoung Kim, Mariette Ducatez, Erika Geijer, Jean-Francois Valarcher, Sara Hägglund, Siamak Zohari

“…Individual samples with a Ct value of <30 for IDV RNA were further analysed by deep sequencing. Phylogenetic analysis was performed by the maximum likelihood estimation method on the whole IDV genome sequence from 16 samples. …”
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Targeting NF-kappaB-inducing kinase shapes B-cell homeostasis in myasthenia gravis by Xiaoyu Huang, Zhouao Zhang, Zhouyi Wang, Tiancheng Luo, Mingjin Yang, Xinyan Guo, Xue Du, Tianyu Ma, Yong Zhang

“…Methods The transcriptome of sorted CD19+B cells obtained from MG patients in active and remission state was performed by RNA sequencing. The hallmark gene NF-kappaB-inducing kinase (NIK/MAP3K14) associated with NF-κB and TNF signaling was identified, and the expression levels of NIK in CD19+B cells, CD4+T cells and serum from new-onset MG patients and controls were validated by flow cytometry, qPCR and ELISA. …”
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Novel protein-based prognostic signature linked to immunotherapeutic efficiency in ovarian cancer by Shuo-Fu Chen, Liang-Yun Wang, Yi-Sian Lin, Cho-Yi Chen

“…Methods The workflow was demonstrated based on the Reverse Phase Protein Array (RPPA) and RNA-sequencing profiles of ovarian cancer patients from The Cancer Genome Atlas (TCGA). …”
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Resiquimod induces a mixed Th1 and Th2 response via STAT1 and STAT3 signalling in chickens by Deepthi Kappala, Saravanan Ramakrishnan, Patel Nikunjkumar Prakashbhai, Abinaya Kaliappan, Prasad Thomas, Jörg Linde, Mithilesh Singh, Sohini Dey, Madhan Mohan Chellappa

“…Pooled splenocytes harvested from chickens (n = 2/group) at 24 h post R-848 treatment were subjected to RNA sequencing and significant differentially expressed genes (DEGs) were identified compared to controls. …”
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Anti-allergic effect of 5,7-dihydroxy-4-methylcoumarin in IgE-mediated RBL-2H3 cells and PCA murine model by Sugeun Gwon, Seong-Ah Shin, Moonsu Kim, Sun Young Moon, Seyeon Choi, Minji Kim, Jun Hyuck Lee, Hyun Ho Park, Chang Sup Lee

“…Our findings demonstrated that 5,7-dihydroxy-4-methylcoumarin reduced the release of histamine and β-hexosaminidase and downregulated the mRNA expression of allergic-inflammatory cytokines, such as interleukin (IL)-4, IL-13, and tumor necrosis factor-alpha, as well as the inflammatory enzyme cyclooxygenase-2. …”
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CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments by Yiqiang Sun, Weiwei Zhang, Houyun Zhang, Feng Zhao, Laijin Su

“…Here, we reverse transcribed viral RNA into cDNA, and then used the constructed RPA-CRISPR/Cas13a-based platform for sensitive and quantitative monitoring of NoV GII.4 in aqueous environments. …”
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