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4481
Ocular Findings in COVID-19 Patients: A Review of Direct Manifestations and Indirect Effects on the Eye
Published 2020-01-01“…While diagnostic and therapeutic efforts have been focused on respiratory complications of the disease, several ocular implications have also emerged. SARS-CoV-2 RNA has been found in tears of the infected patients, and reports suggest that the ocular surface could serve as a portal of entry and a reservoir for viral transmission. …”
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4482
AcfA Regulates the Virulence and Cell Envelope Stress Response of <i>Vibrio parahaemolyticus</i>
Published 2024-12-01“…Additionally, AcfA was involved in adapting to stressors, such as H<sub>2</sub>O<sub>2</sub>, EDTA, and acid, in <i>V. parahaemolyticus</i>. Furthermore, RNA-Seq transcriptome analysis was conducted to identify global gene transcription alterations resulting from deletion of the <i>acfA</i> gene. …”
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4483
Newcastle disease virus genotype VII.1.1 identified from backyard chickens with low antibody titer: Jimma Zone, Southwest Ethiopia.
Published 2025-01-01“…From the swab samples, total RNA was extracted and the viral genomic material was detected by amplifying the Fusion gene by Reverse transcription polymerase chain reaction (RT-PCR). …”
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4484
Metagenomic analysis post thermal stress in buffalo sludge and tomato pomace anaerobic digestion: a case study
Published 2023-11-01“…The bacterial and archaeal communities were an-alyzed using next-generation sequencing of 16S rRNA gene amplicons. After seven days of fermentation, due to the high volatile solid content in TP (77 g/L), the pH dropped to a value of 5, and the methane production reached a plateau (860 Nml of cumulative CH4 production). …”
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4485
Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
Published 2013-01-01“…., tumor necrosis factor-α, interleukin- (IL-) 1β, IL-6, and macrophage inflammatory protein-2) in bronchoalveolar lavage fluid and of their mRNA expression in lung tissues. ISO also inhibited ZY-induced expression and activation of nuclear factor-kappaB p65 and inducible nitric oxide synthase in pulmonary tissue. …”
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4486
Genomic insights into a multidrug-resistant pandoraea apista clinical isolate carrying blaOXA-153 from China
Published 2025-03-01“…Results: The genome sequence of P. apista strain PA167 comprises 5,580,873 bp, with 4,926 protein-coding sequences, 4 ncRNAs, 59 tRNAs, and 3 rRNA operons. Only one ARG was identified: blaOXA-153. …”
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4487
Wet Feeding Promotes Growth without Affecting Hypothalamic Peptide Gene Expression in Growing Broiler Chicks
Published 2025-02-01“…No significant changes were observed in mRNA levels of the genes encoding appetite-regulatory peptides (neuropeptide Y and α melanocyte-stimulating hormone), osmoregulatory peptides (vasotocin and mesotocin), or stress-related peptides (corticotrophin-releasing factor) in the chicken hypothalamus. …”
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4488
Combination of rapamycin and adipose-derived mesenchymal stromal cells enhances therapeutic potential for osteoarthritis
Published 2025-01-01“…In addition, rapamycin improved AD-MSC immunoregulatory functions by promoting the expression of immunosuppressive factors, such as IDO1, PTGS2 and also CD274 (encoding PD-L1). Finally, RNA sequencing analysis showed that in the presence of rapamycin, AD-MSCs displayed improved chondroprotective regenerative effects on co-cultured OA chondrocytes. …”
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4489
Taxonomic structure of bacterial communities in sourdoughs of spontaneous fermentation
Published 2022-07-01“…To study the taxonomic structure of the sourdough microbiome in dynamics, the method of high-throughput sequencing of 16S rRNA gene fragments of microorganisms was used. It was shown that the technological parameters of sourdough (humidity, temperature) do not affect the taxonomic composition of the microbiome of dense rye or liquid rye sourdough at the phylum/class/genus level. …”
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4490
Obesity-induced mesenteric PVAT remodelling is sexually dimorphic, but not driven by ovarian hormones
Published 2025-01-01“…Mesenteric PVAT (mPVAT) was isolated for RNA-sequencing and histological analysis, and mesenteric arteries were isolated for assessment of vascular function by wire myography. …”
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4491
Hepatocyte Rho-associated kinase signaling is required for mice to survive experimental porphyria-associated liver injury
Published 2025-02-01“…DDC-injured DKO mice demonstrated markedly distorted liver histology characterized by large cavities in the parenchyma. RNA-seq analysis showed upregulation of cell death, inflammatory, and profibrotic pathways in DDC-injured DKO liver as compared to DDC-injured WT liver. …”
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4492
Trichinella spiralis excretory/secretory proteins mediated larval invasion via inducing gut epithelial apoptosis and barrier disruption.
Published 2025-01-01“…The results of trans-epithelial electrical resistance (TEER) and FITC-dextran showed that IIL ESP decreased the TEER, increased FITC-dextran flux in Caco-2 monolayer. qPCR, Western blot and immunofluorescence test (IFT) showed that IIL ESP decreased the mRNA and protein expression of TJs (ZO-1, E-cad, Occludin and Claudin-1). …”
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4493
Extracellular electron transfer genes expressed by candidate flocking bacteria in cable bacteria sediment
Published 2025-01-01“…Here, we elucidate the electron exchange capabilities of the bacterial community by coupling metagenomics and metatranscriptomics to 16S rRNA amplicon-based correlations with cable bacteria over 155 days. …”
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4494
Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway
Published 2019-01-01“…The expression levels of glial fibrillary acidic protein (GFAP), ionized calcium binding adapter molecule 1 (iba-1), and toll-like receptor 4 (TLR4) were assessed via immunohistochemistry, real-time quantitative PCR, and Western blot. RNA and proteins were extracted 14 days following transplantation, and the expression levels of the TLR4 signaling pathways and proinflammatory cytokines—myeloid differentiation factor 88 (MyD88), IL-1β, IL-6, and TNF-α—were determined. …”
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4495
Effect of Continuous Feeding of Ayu-Narezushi on Lipid Metabolism in a Mouse Model of Metabolic Syndrome
Published 2021-01-01“…Serum triglyceride and cholesterol levels were significantly lower in the Ayu-narezushi group than in the control group at 24 weeks of age. Furthermore, hepatic mRNA levels of carnitine-palmitoyl transferase 1 and acyl-CoA oxidase, which related to fatty acid oxidation, were significantly increased in the ayu-narezushi group than in the control group at 24 weeks of age. …”
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4496
Polytrauma in Older Adults Leads to Significantly Increased TIMP-1 Levels in the Early Posttraumatic Period
Published 2020-01-01“…MMP-9 and TIMP-1 have been found to play a major role in posttraumatic immune disorder in a previous genome-wide mRNA analysis. Objective. The aim of this study was to evaluate the differences in serum protein dynamics in older and younger polytraumatized adults. …”
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4497
THE DEVELOPMENT OF SCIENCE AND THE FATE OF A SCIENTIST (FOLLOWING GENETICS)
Published 1997-01-01“…., 1975–1977, "The DNA (RNA) Nucleotide Sequence–The Function." One can notice that a change of levels occurs at almost every 30-year period. …”
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4498
The Effects of Hyperacute Serum on the Elements of the Human Subchondral Bone Marrow Niche
Published 2018-01-01“…Monolayer MSCs showed significantly higher metabolic activity following 5 days’ incubation in HAS, and osteoblast-specific mRNA expression was markedly increased, while cells also retained their MSC-specific cell surface markers. …”
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4499
The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro
Published 2014-01-01“…In this study we found that osthole exerted a potent ability to promote proliferation and osteogenic function of rat bone marrow stromal cells and osteoblasts, including improved cell viability, alkaline phosphatase activity, enhanced secretion of collagen-I, bone morphogenetic protein-2, osteocalcin and osteopontin, stimulated mRNA expression of insulin-like growth factor-1, runt-related transcription factor-2, osterix, OPG (osteoprotegerin), RANKL (receptor activator for nuclear factor-κB ligand), and the ratio of OPG/RANKL, as well as increasing the formation of mineralized nodules. …”
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4500
FOSL2 activates TGF‐β1‐mediated GLUT1/mTOR signaling to promote diabetic kidney disease
Published 2025-02-01“…Knockdown of FOSL2 reduced the mRNA expression of TGF‐β1 to decrease the protein expression of GLUT1 and mTOR in the kidney tissues of DKD mice, and TGF‐β1 reversed the effects caused by knockdown of FOSL2. …”
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