Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKKβ-AMPK-SIRT1 Signaling Pathway by Xin Liu, Ning Wang, Yuanfeng Zhu, Yongjun Yang, Xiaoli Chen, Shijun Fan, Qian Chen, Hong Zhou, Jiang Zheng

“…Chemical inhibitor or siRNA of SIRT1 enhanced IL-12 release while its agonist suppressed IL-12 production. …”
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Mgl2+ cDC2s coordinate fungal allergic airway type 2, but not type 17, inflammation in mice by Peter C. Cook, Sheila L. Brown, Emma L. Houlder, Julio Furlong-Silva, Daniel P. Conn, Stefano A. P. Colombo, Syed Baker, Freya R. Svedberg, Gareth Howell, Margherita Bertuzzi, Louis Boon, Joanne E. Konkel, Christopher R. Thornton, Judith E. Allen, Andrew S. MacDonald

“…Here we find that CD11c+ DCs and CD4+ T cells are essential for development of both type 2 and type 17 airway inflammation in mice repeatedly exposed to inhaled spores. Single cell RNA-sequencing with further multi-parameter cytometry shows that allergic inflammation dramatically alters the proportion of numerous DC clusters in the lung, but that only two of these (Mgl2+ cDC2s and CCR7+ DCs) migrate to the dLNs. …”
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Genome-wide analysis of Smad and Schnurri transcription factors in C. elegans demonstrates widespread interaction and a function in collagen secretion by Mehul Vora, Jonathan Dietz, Zachary Wing, Karen George, Jun Kelly Liu, Christopher Rongo, Cathy Savage-Dunn

“…Here, we identify functional binding sites for SMA-3/Smad and its transcriptional partner SMA-9/Schnurri based on ChIP-seq peaks (identified by modEncode) and expression differences of nearby genes identified from RNA-seq analysis of corresponding mutants. We found that SMA-3 and SMA-9 have both overlapping and unique target genes. …”
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Immunological Aspects of Fulminant Type 1 Diabetes in Chinese by Zhen Wang, Ying Zheng, Yiting Tu, Zhijie Dai, Jian Lin, Zhiguang Zhou

“…And peripheral T cell responses to GAD65, insulin B9–23 peptide, or C peptide were determined in 10 FT1D patients and 10 healthy controls. The mRNA levels of several related cytokines and molecules, such as IFN-γ, IL-4, RORC, and IL-17 in PBMCs from FT1D patients were analyzed by qRT-PCR. …”
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Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1β, and Tumor Necrosis Factor-α via a NF-κB-Dependent Mechanism in HaCaT Keratinocytes by Bing-rong Zhou, Jia-an Zhang, Qian Zhang, Felicia Permatasari, Yang Xu, Di Wu, Zhi-qiang Yin, Dan Luo

“…Secretion levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), NF-κB nuclear translocation, NF-κB activation, Stat3 phosphorylation, and peroxisome proliferator-activated receptor alpha (PPARα) mRNA and protein levels, as well as the cell proliferation ability were measured at the end of the treatment and after 24 hours of recovery. …”
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Preparation and In Vivo Expression of CS-PEI/pCGRP Complex for Promoting Fracture Healing by Chun-Liang Li, Feng Qin, Rong-rong Li, Jia Zhuan, Hai-Yong Zhu, Yu Wang, Kai Wang

“…Moreover, CS-PEI/pCGRP significantly enhanced the mRNA and protein levels of downstream RUNX2 and ALP. …”
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Supplementation with Complex Phytonutrients Enhances Rumen Barrier Function and Growth Performance of Lambs by Regulating Rumen Microbiome and Metabolome by Juan Du, Yuan Wang, Shaohui Su, Wenwen Wang, Tao Guo, Yuchao Hu, Na Yin, Xiaoping An, Jingwei Qi, Xuan Xu

“…Our findings suggest that adding CPS to lambs’ diets has widespread positive impacts, including improved growth performance, reduced inflammation and mRNA relative expression of apoptosis-related genes, enhanced barrier function, and beneficial changes in the rumen microbiome and metabolite profiles.…”
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CTLA-4 and PD-1 Ligand Gene Expression in Epithelial Thyroid Cancers by Chiara Tuccilli, Enke Baldini, Salvatore Sorrenti, Antonio Catania, Alessandro Antonelli, Poupak Fallahi, Francesco Tartaglia, Susi Barollo, Caterina Mian, Andrea Palmieri, Giovanni Carbotta, Stefano Arcieri, Daniele Pironi, Massimo Vergine, Massimo Monti, Salvatore Ulisse

“…Here, the expression of PD-L1, PD-L2, CD80, and CD86 was evaluated at the mRNA level in 94 patients affected by papillary thyroid carcinoma (PTC) and 11 patients affected by anaplastic thyroid carcinoma (ATC). …”
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Occurrence and Multi-Locus Genotyping of <i>Giardia duodenalis</i> in Black Goats from Fujian Province, China by Shou-Xiao Huang, Kai Hu, Peng-Fei Fu, Si-Ang Li, Yang Liu, Zhipeng Niu, Dong-Hui Zhou

“…The presence of <i>G. duodenalis</i> was confirmed through nested PCR targeting the SSU rRNA gene, and genotyping was performed at the beta-giardin, glutamate dehydrogenase, and triosephosphate isomerase loci. …”
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Preferential Expression of B7-H6 in Glioma Stem-Like Cells Enhances Tumor Cell Proliferation via the c-Myc/RNMT Axis by Hanqing Chen, Yundi Guo, Jing Sun, Jun Dong, Qinghua Bao, Xueguang Zhang, Fengqing Fu

“…Moreover, we determined that three genes CBL (Casitas B-Lineage Lymphoma Proto-Oncogene), CCNT1 (Cyclin T1), and RNMT (RNA guanine-7 methyltransferase) were coexpressed with B7-H6 and c-myc in glioma tissue samples from the TCGA database and found, however that only RNMT expression was inhibited by the knockdown of B7-H6 expression in the GSLCs, suggesting the involvement of RNMT in the B7-H6/c-myc axis. …”
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Targeting OAS3 for reversing M2d infiltration and restoring anti-tumor immunity in pancreatic cancer by Shaopeng Zhang, Ximo Xu, Kundong Zhang, Changzheng Lei, Yitian Xu, Pengshan Zhang, Yuan Zhang, Haitao Gu, Chen Huang, Zhengjun Qiu

“…Increased OAS3 expression in TAMs correlates with m6A modification mediated by METTL3 on OAS3 mRNA and is associated with poorer prognosis in PC patients. …”
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Variations in rumen microbiota and host genome impacted feed efficiency in goat breeds by Alaa Emara Rabee, Ibrahim Abou-souliman, Ahmed I. Yousif, Mebarek Lamara, Mohamed A. El-Sherbieny, Eman Ali Elwakeel, Ahmed M. Sallam

“…The animals were genotyped using the Illumina 65KSNP chip arrays v2, and their rumen bacteria and archaea were investigated using PCR-amplicon sequencing of the 16S rRNA gene.ResultsThe results showed that the ZA goats exhibited higher FE than the SH goats (p &lt; 0.05) and a greater relative abundance (p &lt; 0.05) of rumen bacterial groups that are involved in the degradation of cellulose and hemicelluloses, such as Bacteroidota and Fibrobacterota, along with genera such as Prevotella, Lachnospiraceae AC2044 group, Lachnospiraceae NK3A20 group, and Succiniclasticum, which are linked to carbohydrate and nitrogen metabolism pathways. …”
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Low-Dose Mitomycin C Decreases the Postoperative Recurrence Rate of Pterygium by Perturbing NLRP3 Inflammatory Signalling Pathway and Suppressing the Expression of Inflammatory Fac... by Qie Guo, Xiao Li, Meng-Na Cui, Yu Liang, Xiang-Peng Li, Jun Zhao, Li-Na Wei, Xiao-Lei Zhang, Xiang Hua Quan

“…Mitomycin C (MMC) is an antineoplastic antibiotic that inhibits DNA, RNA, and protein synthesis. In recent years, although MMC has proven useful for the treatment of pterygium, its application has been controversial because of its clear toxicity and the possibility of ocular complications. …”
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Impaired fracture healing is associated with callus chondro-osseous junction abnormalities in periostin-null and osteopontin-null mice by Marc Teitelbaum, Marc Teitelbaum, Maya D. Culbertson, Charlene Wetterstrand, J. Patrick O’Connor, J. Patrick O’Connor

“…Apparent changes in specific protein levels identified by immunohistochemistry were confirmed by mRNA quantitation. Comparisons between the PostnKO and Spp1KO fracture calluses were confounded by interactions between the two genes; loss of Postn reduced Spp1 expression and loss of Spp1 reduced Postn expression. …”
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Single-cell sequencing provides clues about the developmental genetic basis of evolutionary adaptations in syngnathid fishes by Hope M Healey, Hayden B Penn, Clayton M Small, Susan Bassham, Vithika Goyal, Micah A Woods, William A Cresko

“…We created a single-cell RNA sequencing atlas of Gulf pipefish embryos to understand the developmental basis of four traits: derived head shape, toothlessness, dermal armor, and male pregnancy. …”
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Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and Their Sheets on Poly-ε-Caprolactone/β-Tricalcium Phosphate Composite Scaffolds in Canine Bone Defects... by Yongsun Kim, Seung Hoon Lee, Byung-jae Kang, Wan Hee Kim, Hui-suk Yun, Oh-kyeong Kweon

“…The ALP, runt-related transcription factor 2, osteopontin, and bone morphogenetic protein 7 mRNA levels were upregulated in O-MSCs and OCS as compared to U-MSCs. …”
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Mechanistic elucidation of human pancreatic acinar development using single-cell transcriptome analysis on a human iPSC differentiation model by Atsushi Mima, Azuma Kimura, Ryo Ito, Yu Hatano, Hiraku Tsujimoto, Shin-Ichi Mae, Junko Yamane, Wataru Fujibuchi, Norimitsu Uza, Taro Toyoda, Hiroshi Seno, Kenji Osafune

“…In this study, we aimed to elucidate the developmental mechanisms of pancreatic exocrine acinar lineages to establish a directed differentiation method for pancreatic acinar cells from hiPSCs. hiPSC-derived pancreatic endoderm cells were spontaneously differentiated into both pancreatic exocrine and endocrine tissues by implantation into the renal subcapsular space of NOD/SCID mice. Single-cell RNA-seq analysis of the retrieved grafts confirmed the differentiation of pancreatic acinar lineage cells and identified REG4 as a candidate marker for pancreatic acinar progenitor cells. …”
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Curcumin nanoparticles alleviate brain mitochondrial dysfunction and cellular senescence in γ-irradiated rats by Omnia A. Moselhy, Nahed Abdel-Aziz, Azza El-bahkery, Said S. Moselhy, Ehab A. Ibrahim

“…Moreover, CNPs administered to irradiated rats significantly reduced brain β-galactosidase activity and the expression levels of p53, p21, and p16 genes (P < 0.05) while concurrently inducing a significant increase in AMPK mRNA expression compared to the irradiated group. …”
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TET2-mediated 5-hydroxymethylcytosine of TXNIP promotes cell cycle arrest in systemic anaplastic large cell lymphoma by Ziqing Yu, Lihua Zhong, Wangyang Tang, Wenfang Zhang, Ting Lin, Weifeng Zhu, Gang Chen, Jianchao Wang

“…The ALK-positive cell line, Su-DHL-1, and the ALK-negative cell line, DL-40, were utilized as in vitro experimental models. RNA-sequencing and hMeDIP-sequencing assays were performed to explore the potential functions of TET2 in cell cycle regulation. …”
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Pretransplant Immune- and Apoptosis-Related Gene Expression Is Associated with Kidney Allograft Function by Dorota Kamińska, Katarzyna Kościelska-Kasprzak, Paweł Chudoba, Oktawia Mazanowska, Mirosław Banasik, Marcelina Żabinska, Maria Boratyńska, Agnieszka Lepiesza, Agnieszka Gomółkiewicz, Piotr Dzięgiel, Marian Klinger

“…Immediate posttransplant graft function (14-day GFR) was influenced negatively by TGFB1 (P=0.039) and positively by IL-2 gene expression (P=0.040). Pretransplant blood mRNA expression of apoptosis-related genes (CASP3, FAS, and IL-18) and Th1-derived cytokine gene IFNG correlated positively with short- (6-month GFR CASP3: P=0.027, FAS: P=0.021, and IFNG: P=0.029) and long-term graft function (24-month GFR CASP3: P=0.003, FAS: P=0.033, IL-18: P=0.044, and IFNG: P=0.04). …”
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