Quantitative Detection of miRNA-21 Expression in Tumor Cells and Tissues Based on Molecular Beacon by Qingxin Liu, Jialong Fan, Chuang Zhou, Liqun Wang, Bin Zhao, Haibin Zhang, Bin Liu, Chunyi Tong

“…As a new tumor marker, the microRNA-21 (miRNA21) level can provide important information for early diagnosis, drug treatment, and prognosis of gastric cancer. …”
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Mono-allelic epigenetic regulation of polycistronic transcription initiation by RNA polymerase II in Trypanosoma brucei by Rudo Kieft, Laura Cliffe, Haidong Yan, Robert J. Schmitz, Stephen L. Hajduk, Robert Sabatini

“…Here, we demonstrate that by in vitro selection with TLF, resistance is obtained in a stepwise process correlating with loss of HpHbR expression at an allelic level. RNA-seq, Pol II ChIP, and run-on analysis indicate HpHbR silencing is at the transcriptional level, where loss of Pol II binding at the promoter region specifically shuts down transcription of the HpHbR-containing gene cluster and the adjacent opposing gene cluster. …”
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Molecular Diagnosis of Periprosthetic Joint Infection by Quantitative RT-PCR of Bacterial 16S Ribosomal RNA by Mel S. Lee, Wen-Hsin Chang, Su-Chin Chen, Pang-Hsin Hsieh, Hsin-Nung Shih, Steve W. N. Ueng, Gwo-Bin Lee

“…This study used quantitative reverse transcription polymerase chain reaction (RT-qPCR) to amplify bacterial 16S ribosomal RNA in vitro and in 11 clinical samples. The in vitro analysis demonstrated that the RT-qPCR method was highly sensitive with the detection limit of bacterial 16S rRNA being 0.148 pg/μl. …”
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A semi-automatic cell type annotation method for single-cell RNA sequencing dataset by Wan Kim, Sung Min Yoon, Sangsoo Kim

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MicroRNA-451a is a candidate biomarker and therapeutic target for major depressive disorder by Yan Chen, Yun Liu, Ming Xiao, Chun Wang, Panpan Hu, Qiuchen Cao, Hu Feng, Jingfan Xu, Weixi Feng, Huaiqing Sun, Huachen Ding, Junying Gao

“…Finally, molecular biological experiments were conducted to investigate the potential mechanism of miR-451a against depression.Results The serum levels of miRNA-451a were significantly lower in patients with MDD, with a negative correlation with the Hamilton Depression Scale scores. …”
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MicroRNA-221 protects myocardial contractility in myocardial ischemia/reperfusion injury through phospholamban. by Hongyu Li, Jimiao Qiu, Chang Liu, Guobing Yu, Danyu Wu, Yichun Chu, Kai Wang

“…<h4>Objective</h4>To investigate the effects and mechanisms of miRNA 221 on myocardial ischemia/reperfusion injury (MIRI) in mice through the regulation of phospholamban (PLB) expression.…”
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LncRNA MANCR is downregulated in non-small cell lung cancer and predicts poor survival by Yunming Tao, Jie Liu, Wenxiao Qiu, Yuanyuan Li

“…Mitotically associated long non-coding RNA (MANCR) has been reported to promote genomic stability, suggesting its involvement in cancers. …”
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Single cell RNA sequencing improves the next generation of approaches to AML treatment: challenges and perspectives by Zahra Khosroabadi, Samaneh Azaryar, Hassan Dianat-Moghadam, Zohreh Amoozgar, Mohammadreza Sharifi

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Pathogenic PDE12 variants impair mitochondrial RNA processing causing neonatal mitochondrial disease by Lindsey Van Haute, Petra Páleníková, Jia Xin Tang, Pavel A Nash, Mariella T Simon, Angela Pyle, Monika Oláhová, Christopher A Powell, Pedro Rebelo-Guiomar, Alexander Stover, Michael Champion, Charulata Deshpande, Emma L Baple, Karen L Stals, Sian Ellard, Olivia Anselem, Clémence Molac, Giulia Petrilli, Laurence Loeuillet, Sarah Grotto, Tania Attie-Bitach, Jose E Abdenur, Robert W Taylor, Michal Minczuk

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Elucidating the molecular basis of salt tolerance in potatoes through miRNA expression and phenotypic analysis by Caicai Lin, Shuangshuang Zheng, Kui Liu, Ru Yu, Peiyan Guan, Baigeng Hu, Lingling Jiang, Mengyu Su, Guodong Hu, Qingshuai Chen, Xia Zhang

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RNA splicing: a split consensus reveals two major 5′ splice site classes by Matthew T. Parker, Sebastian M. Fica, Gordon G. Simpson

“…The established consensus sequence for human 5′ splice sites masks the presence of two major splice site classes defined by preferential base-pairing potentials with either U5 snRNA loop 1 or the U6 snRNA ACAGA box. The two 5′ splice site classes are separable in genome sequences, sensitized by specific genotypes and associated with splicing complexity. …”
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Correlation between the Frequency of Th17 Cell and the Expression of MicroRNA-206 in Patients with Dermatomyositis by Xinyi Tang, Xinyu Tian, Yue Zhang, Wei Wu, Jie Tian, Ke Rui, Jia Tong, Liwei Lu, Huaxi Xu, Shengjun Wang

“…In this study, we investigated the frequency of Th17 cells and the expression of microRNA-206 (miR-206) in DM patients. Firstly, we observed that the frequency of Th17 cells and the expression of transcription factors were increased significantly in the PBMCs of DM patients. …”
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RNA modification writer-based immunological profile and genomic landscape of tumor microenvironment in lung adenocarcinoma by Qiang Xu, Lingyu Kong, Zhezhu Han, Xiuying Jin, Mingyan Ding, Zhengri Piao, Songnan Zhang

Subjects: “…RNA modification writer…”
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MicroRNA in Cervical Cancer: OncomiRs and Tumor Suppressor miRs in Diagnosis and Treatment by Kouji Banno, Miho Iida, Megumi Yanokura, Iori Kisu, Takashi Iwata, Eiichiro Tominaga, Kyoko Tanaka, Daisuke Aoki

“…Previous approaches to development of siRNA as a drug have provided information for establishment of therapy based on these approaches, and an anti-miR-21 inhibitor has been developed. miRNAs also have effects on drug resistance and may be useful in combination therapy with other drugs.…”
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Cardiovascular Disease, Single Nucleotide Polymorphisms; and the Renin Angiotensin System: Is There a MicroRNA Connection? by Terry S. Elton, Sarah E. Sansom, Mickey M. Martin

“…MicroRNAs (miRNAs) are a family of small, ∼21-nucleotide long, and nonprotein-coding RNAs that recognize target mRNAs through partial complementary elements in the 3-untranslated region (3-UTR) of mRNAs and inhibit gene expression by targeting mRNAs for translational repression or destabilization. Since miRNA SNPs (miRSNPs) can create, destroy, or modify miRNA binding sites, this review focuses on the hypothesis that transcribed target SNPs harbored in RAS mRNAs, that alter miRNA gene regulation and consequently protein expression, may contribute to cardiovascular disease susceptibility.…”
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An Integrating Approach for Genome-Wide Screening of MicroRNA Polymorphisms Mediated Drug Response Alterations by Xianyue Wang, Hong Jiang, Wei Wu, Rongxin Zhang, Lingxiang Wu, Huan Chen, Pengping Li, Yumin Nie, Jiaofang Shao, Yan Li, Xue Lin, Sali Lv, Qh Wang, Jie Hu

“…Genetic polymorphisms in miRNA target sites may alter miRNA regulation and therefore result in the alterations of the drug targets. …”
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Senescence-related circRNA circHIF-1α is associated with pancreatic cancer progression by Hao Hua, Yazu Deng, Dijie Zheng, Changhao Wu, Liwen Chen, Binbin Shi, Zhiwei He, Chao Yu

“…Therefore, we identified circHIF-1α, hsa_circ_0007976, which was downregulated in senescent cells using circRNA microarray analysis. Meanwhile, significantly upregulated expression of circHIF-1α in pancreatic cancer tissue detected by reverse transcription-polymerase chain reaction (RT-qPCR) and in situ hybridization (ISH). …”
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Epitranscriptomic RNA m6A Modification in Cancer Therapy Resistance: Challenges and Unrealized Opportunities by Mohammad Burhan Uddin, Zhishan Wang, Chengfeng Yang

“…Epitranscriptomics, especially related to m6A RNA modification dysregulation in cancer, is an emerging mechanism for cancer therapy resistance. …”
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T14diLys/DOPE Liposomes: An Innovative Option for siRNA-Based Gene Knockdown? by Sophie Meinhard, Frank Erdmann, Henrike Lucas, Maria Krabbes, Stephanie Krüger, Christian Wölk, Karsten Mäder

Subjects: “…siRNA…”
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Innovative Lipid Nanoparticles Co-Delivering Hydroxychloroquine and siRNA for Enhanced Rheumatoid Arthritis Therapy by Yanru Feng, Xintong Pan, Ziqian Li, Yue Li, Ya’nan Sun, Shaokun Yang, Chaoxing He, Yunjie Dang, Lu Huang, Bai Xiang

“…<b>Methods:</b> In this study, we introduce a novel lipid nanoparticle formulation co-delivering hydroxychloroquine (HCQ) and siRNA targeting TNF-α (si<i>TNF-α</i>) using microfluidic technology, marking the first use of such a combination for RA therapy. …”
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