Comparative Analysis of the Anti-inflammatory Effects of E-MTA, Ketac-E, and Sealapex on Human Periodontal Ligament Stem Cells by Ayesha Imtiaz, Kiran Nayyar, Khadija Akhtar, Muhammad Yousuf Ali, Naresh kumar, Ehsan Ul Haq

“…Expression profiling via Real Time-PCR of anti-inflammatory biometers was determined via RNA extract from 24h MTT assay due to its greater reproducibility. …”
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A practical distribution pattern of α-SMA-positive carcinoma associated fibroblasts indicates poor prognosis of patients with pancreatic ductal adenocarcinoma by Bo Li, Meilong Shi, Yang Wang, Penghao Li, Xiaoyi Yin, Guoxiao Zhang, Xiaochao Kang, Huan Wang, Suizhi Gao, Kailian Zheng, Xiaohan Shi, Xiongfei Xu, Yukun Zhou, Hui Jiang, Wei Jing, Shiwei Guo, Gang Jin

“…Multivariable Cox proportional hazards regression was used to assess risk factors associated with patient outcomes. RNA sequencing data were obtained from bulk tumor samples and isolated CAFs from patients to reveal the distinct pattern and elucidated their fundamental characteristics.Results The α-SMA spatial intensity was the most suitable variable for representative of CAFs spatial characteristics. …”
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First Detection of <i>Alphacoronavirus</i> in Bats from the World’s Largest Wetland, the Pantanal, Brazil by Tayane B. S. Magalhães, Amanda de O. Viana, Thiago B. F. Semedo, Juliane S. Saldanha, Nicole A. dos Reis, Nathalia de A. Pereira, Rachel V. P. de Barros, Hannah R. Miranda, Gabriella C. Almeida, Desyrée Y. S. R. Ozaki, Giovana S. Caleiro, Gustavo O. Fenner, Fernanda P. Vizu, Theo Kraiser, Thais P. Carvalho, Luciano M. Thomazelli, Erick G. Dorlass, Clarice W. Arns, Helena L. Ferreira, Erika Hingst-Zaher, Rogério Vieira Rossi, Guilherme S. T. Garbino, Edison L. Durigon, Jansen de Araujo, Daniel M. de Aguiar

“…Oral and rectal swabs collected in 2021 from 419 bats were analyzed using Pancoronavirus-nested PCR targeting the RNA-dependent RNA-polymerase (RdRp) gene. Orthocoronavirinae was detected in 16.7% (70/419) of the bats; nine samples were sequenced, confirming that <i>Carollia perspicillata</i> (4), <i>Phyllostomus hastatus</i> (2), <i>Desmodus rotundus</i> (1), <i>Molossus rufus</i> (1), and <i>Myotis</i> cf. …”
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Phylogenetic and pangenomic analyses of members of the family <i>Micrococcaceae</i> related to a plant-growth-promoting rhizobacterium isolated from the rhizosphere of potato (<i>S... by S. Yu. Shchyogolev, G. L. Burygin, L. A. Dykman, L. Yu. Matora

“…We report the results of taxonomic studies on members of the family Micrococcaceae that, according to the 16S rRNA, internal transcribed spacer 1 (ITS1), average nucleotide identity (ANI), and average amino acid identity (AAI) tests, are related to Kocuria rosea strain RCAM04488, a plant-growth-promoting rhizobacterium (PGPR) isolated from the rhizosphere of potato (Solanum tuberosum L.). …”
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Non-invasive imaging with ICOS-targeting monoclonal antibody for preclinical diagnosis of rheumatoid arthritis in a humanized mouse model by Shao Duan, Chao Li, Feng Yan, Yifei Xia, Shuaiming Shao, Weiyu Chen, Zunyu Xiao, Gongping Xu

“…Findings ICOS was confirmed as an indicator of RA pathogenesis via RNA-seq, flow cytometry and immunofluorescence data. …”
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No microorganism was detected in amniotic fluid of healthy pregnancies from the second trimester to the delivery by Yu Liu, Jingmei Ma, Xiang Li, Haijuan Zhao, Qubo Ai, Lanying Zhang, Yulong Tong, Lingzhen Meng, Huixia Yang

“…Microbial profiles were assessed through cultivation, quantitative real-time polymerase chain reaction, 16S ribosomal RNA gene sequencing, and cytokine analysis. Results In cohort 1, the bacterial load and community structure in the second trimester AF samples were indistinguishable from negative controls. …”
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Retrospective evaluation of multiplex PCR panel results from CSF samples in a university hospital by Funda Şahin, Nida Özcan, Erdal Özbek, Selahattin Atmaca, Hakan Temiz

“…Genetic materials (DNA/RNA) related to Herpes simplex virus 1-2, Humman herpesvirus 6-7-8, Varicella zoster virus, Enterovirus, Cytomegalovirus, Human Parechoviruses, Haemophilus influenzae, Listeria monocytogenes, Streptococcus pneumoniae, Neisseria meningitidis, Streptococcus agalactiae, Escherichia coli K1, Cryptococcus gattii/neoformans in cerebrospinal fluid samples were investigated using the Meningitis/Encephalitis RT-qPCR MX-17 Panel (RT-qPCR MX-17S Panel, Bio-Speedy®, Bioeksen, Türkiye) multiplex PCR kit. …”
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Evidence that the domesticated fungus Leucoagaricus gongylophorus recycles its cytoplasmic contents as nutritional rewards to feed its leafcutter ant farmers by Caio Ambrosio Leal-Dutra, Lok Man Yuen, Bruno Augusto Maciel Guedes, Marta Contreras-Serrano, Pedro Elias Marques, Jonathan Zvi Shik

“…We next confirmed that autophagy plays a critical role in gongylidium formation both: (1) in vitro as gongylidium suppression occurred when isolated fungal cultures were grown on media with autophagy inhibitors, and (2) in silico as differential transcript expression (RNA-seq) analyses showed upregulation of multiple autophagy gene isoforms in gongylidia relative to undifferentiated hyphae. …”
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Lactate Ameliorates Kainic Acid-Induced Neuroinflammation and Cognitive Impairment via the Chemokine Signaling Pathway in Mice by Chu X, Ge Y, Geng C, Cao P, Wei P, Fu B, Deng Z, Li Y, Zhao G

“…The differentially gene expression and functional enrichment were analyzed with RNA sequencing.Results: The in vitro lactate intervention reduced the number of apoptotic cells, the release of inflammatory factors, and the expression of vesicular glutamate transporter 1. …”
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Xiaoyao Pills Attenuate Inflammation and Nerve Injury Induced by Lipopolysaccharide in Hippocampal Neurons In Vitro by Yang Fang, Boyu Shi, Xiaobo Liu, Jie Luo, Zhili Rao, Rong Liu, Nan Zeng

“…The expression levels of IL-6, TNF-α, IDO, 5-HT, brain-derived neurotrophic factor, and β-nerve growth factor were detected by enzyme-linked immunosorbent assay. mRNA levels of IL-6, TNF-α, 5-HT1A, IDO-1, brain-derived neurotrophic factor, nerve growth factor, tropomyosin receptor kinase B, tropomyosin receptor kinase A, and cAMP response element-binding protein were detected by reverse transcription-polymerase chain reaction. …”
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The Wnt/β-Catenin Pathway Regulated Cytokines for Pathological Neuropathic Pain in Chronic Compression of Dorsal Root Ganglion Model by Ye Zhang, Dan Zhao, Xutong Li, Beiyao Gao, Chengcheng Sun, Shaoting Zhou, Yanhong Ma, Xuemei Chen, Dongsheng Xu

“…We aimed to investigate whether the Wnt/β-catenin pathway was involved in NP caused by sustaining dorsal root ganglion (DRG) compression with the chronic compression of dorsal root ganglion model (CCD). Our RNA sequencing results showed that several genes related to the Wnt pathway have changed in DRG and spinal cord dorsal horn (SCDH) after CCD surgery. …”
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Novel genes involved in vascular dysfunction of the middle temporal gyrus in Alzheimer’s disease: transcriptomics combined with machine learning analysis by Meiling Wang, Aojie He, Yubing Kang, Zhaojun Wang, Yahui He, Kahleong Lim, Chengwu Zhang, Li Lu

“…Finally, combining bulk RNA sequencing data and two machine learning algorithms (least absolute shrinkage and selection operator and random forest), four characteristic Alzheimer’s disease feature genes were identified: somatostatin (SST), protein tyrosine phosphatase non-receptor type 3 (PTPN3), glutinase (GL3), and tropomyosin 3 (PTM3). …”
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Groundwater denitrification using electro-assisted autotrophic processes: exploring bacterial community dynamics in a single-chamber reactor by Javiera Toledo-Alarcón, Eduardo Ortega-Martinez, Javier Pavez-Jara, Oscar Franchi, Ivan Nancucheo, Héctor Zuñiga-Barra, Jose Luis Campos, David Jeison

“…Nitrate, nitrite, sulfate, total ammoniacal nitrogen, and 16S rRNA analysis of bacterial community, were monitored during BER operation. …”
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Interleukin-1 signaling and CD4+ T cells control B cell recruitment to the lungs in chronic beryllium disease by Joseph M. Gaballa, Caley Valdez, Douglas G. Mack, Faiz Minhajuddin, Masoom Raza, Tabrez A. Mohammad, Allison K. Martin, Andrew Getahun, Charles A. Dinarello, Andrew P. Fontenot, Shaikh M. Atif

“…In BeO-exposed mice, lung B cells down-regulate IgM but showed an increased IgD and CD44 surface expression. Further, RNA sequencing of pulmonary tissue-specific B cells in CBD revealed distinct gene signatures compared to splenic B cells, with increased expression of pathways involved in antigen presentation, tight junction interactions, and interferon signaling. …”
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HTR1D regulates the PI3K/Akt signaling pathway to impact hepatocellular carcinoma development and resistance to sorafenib by Yingai Zhang, Yuting Zhang, Shuai Zhou, Mujeeb Ur Rehman, Fankai Lin, Jianquan Zhang, Hailong Zhou

“…Methods First, the correlation between HTR1D and hepatocellular carcinoma was analyzed using the TCGA database, and the expression level of HTR1D in clinical samples was detected by qPCR. Then the siRNA was transfected into Huh-7 and Hep3B cells, and the cell proliferation ability, colony formation ability, migration and invasion ability were detected with or without sorafenib. …”
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Lactobacillus gasseri LGV03-derived indole-3-lactic acid ameliorates immune response by activating aryl hydrocarbon receptor by Zikang Zhang, Kangdi Zheng, Zhao Zhang, Longbin Cao, Lizhu Lin, Weimin Sun, Feng Qiu

“…L. gasseri LGV03 showed higher potent activities in ameliorating vinorelbine-induced neutropenia, macrophage and T cells deficiency, and significantly enhanced mRNA expressions of cytokines TNF-α, TNF-β and IFN-α. …”
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The Role of SUMO1 Modification of SOX9 in Cartilage Development Stimulated by Zinc Ions in Mice by Na Xue, Jing Zhao, Jing Yin, Liang Liu, Zhong Yang, Shuchao Zhai, Xiyun Bian, Xiang Gao

“…Our investigation was centered on elucidating the impact of zinc deficiency on cartilage maturation by modulating SUMO1 and UBC9 at both the protein and mRNA levels. We administered a regimen inducing zinc deficiency to gravid mice from E0.5 until euthanasia. …”
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Decrease of NAD+ Inhibits the Apoptosis of OLP T Cells via Inducing Mitochondrial Fission by Zhang ZY, Wang F, Zhou G

“…OLP plasma-exposed Jurkat T cells were infected with the Drp1 shRNA virus to investigate the role of mitochondrial fission in OLP T cell apoptosis. …”
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Lipoxin Inhibits Fungal Uptake by Macrophages and Reduces the Severity of Acute Pulmonary Infection Caused by Paracoccidioides brasiliensis by Laura R. R. Ribeiro, Flávio V. Loures, Eliseu F. de Araújo, Cláudia Feriotti, Tânia A. Costa, Carlos Henrique Serezani, Sonia Jancar, Vera L. G. Calich

“…In susceptible mice, MLT treatment led to reduced influx of PMN leukocytes, increased recruitment of monocytes, predominant synthesis of anti-inflammatory cytokines, and augmented expression of 5- and 15-lipoxygenase mRNA, suggesting a prevalent LXA4 activity. In agreement, MTL-treated macrophages showed reduced fungal burdens associated with decreased ingestion of fungal cells. …”
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Inhibiting the alternative pathway of complement by reducing systemic complement factor B: Randomized, double-blind, placebo-controlled phase 1 studies with Sefaxersen by Michael L. McCaleb, Steven G. Hughes, Tamar R. Grossman, Ashley Frazer-Abel, Bill Jung, Lixuan Yin, Scott P. Henry, Brett P. Monia, Eugene Schneider, Richard Geary, Gary T. Brice

“…In first-in-human double-blind, placebo-controlled phase 1 studies, the safety and pharmacodynamic effects of sefaxersen (RO7434656), a GalNAc-conjugated 2’-MOE antisense oligonucleotide targeting the complement factor B mRNA, was investigated. Healthy volunteers received either single or repeated (for 6 weeks) subcutaneous administrations of investigational drug or placebo. …”
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