Unraveling the Beneficial Role of Resveratrol in Fructose-Induced Non-Alcoholic Steatohepatitis with a Focus on the AMPK/Nrf2 Signaling Axis by Soha S. Zakaria, Safaa M. Hanafy

“…Quantitative real-time PCR was used to assess the mRNA expression of nuclear factor E2-related factor 2 (Nrf2) in the liver samples. …”
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<i>Vibrio cholerae</i> Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response by Isabella Cubillejo, Kevin R. Theis, Jonathan Panzer, Xixia Luo, Shreya Banerjee, Ryan Thummel, Jeffrey H. Withey

“…We also characterized the larval gut microbiome using 16S rRNA sequencing. <i>V. cholerae</i>-infected or uninfected control groups were exposed to either an alternating light/dark stimuli or a single-tap stimulus, and average distance and velocity were tracked. …”
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Comparative Evaluation of Bandage Contact Lenses and Eye Patching after Bilateral Cataract Surgery by Dalan Jing, Aihua Deng, Hongmei Wang, Yilin Chou, Xiaodan Jiang, Zhenxiang Chen, Xuemin Li, Tingyi Wen

“…Moreover, bacterial species in the conjunctival sac, meibomian gland secretions, and BCLs were subsequently identified using 16S rRNA gene sequencing. Results. The patient satisfaction scores of Group A were higher than Group B. …”
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Nanodrug‐Engineered Exosomes Achieve a Jointly Dual‐Pathway Inhibition on Cuproptosis by Hanxiao Sun, Yang Zou, Zhengtai Chen, Yan He, Kai Ye, Huan Liu, Lihong Qiu, Yufan Zhang, Yuexue Mai, Xinghong Chen, Zhengwei Mao, Wei Wang, Chenggang Yi

“…A Cu‐chelator (Clioquinol, CQ) polydopamine (PDA)‐modified stem cell exosome loaded with siRNA‐FDX1, named EXOsiFDX1‐PDA@CQ, is designed to efficiently inhibit the two cuproptosis pathways. …”
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Key wound healing genes as diagnostic biomarkers and therapeutic targets in uterine corpus endometrial carcinoma: an integrated in silico and in vitro study by Fuchuan Jiang, Sajjad Ahmad, Sadia kanwal, Yasir Hameed, Qian Tang

“…Promoter methylation analysis showed significantly higher methylation levels in UCEC, correlating with decreased mRNA expression and poor survival outcomes. Genetic alteration analysis indicated frequent mutations in FN1 and KDM6A, although these did not significantly affect survival. …”
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Unravelling the TCRβ repertoire: a key to unlocking the immunopathogenesis and precision medicine in SLE by Qiao Zhou, Li Zeng, Zhixin Zhang, Jian Liu, Yichen Zhang, Yang An, Lijing Yang, Tianzuo Lan, Pengming He, Xueping Wen, Shaoping Deng

“…Our study aimed to delineate the variances in the TCRβ repertoire between patients with SLE and healthy individuals, correlating these differences with the severity and subtypes of SLE.Methods We conducted an analysis of blood samples from 50 treatment-naive patients with SLE and 50 healthy donors, employing RNA extraction, high-throughput sequencing and subsequent bioinformatics analysis.Results Our findings revealed significant alterations in TRBV and TRBJ gene usage frequencies, indicative of a skewed TCR repertoire in patients with SLE. …”
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Screening and Evaluation of Actinomycetes B-35 from Rhizosphere of <i>Salvia miltiorrhiza</i> Bunge and Its Biocontrol and Growth-Promoting Effects by Mingli Han, Jing Wang, Hang Lin, Chen Yang, Jing Zhang, Jiawei Liu, Guojun Pan, Na Xiao

“…</i> The strain B-35 with the strongest antifungal activity was screened out through antagonizing the pathogen <i>Fusarium solani</i> of <i>S. miltiorrhiza</i>, strain morphology and 16S rRNA analysis. The antagonistic actinomycetes fermentation filtrate and crude extract could significantly destroy the mycelium and spores of <i>Fusarium solani</i>; the biocontrol effect of mature <i>S. miltiorrhiza</i> rhizome reached 83.3%, and the number of leaves, plant height and biomass in the B-35 treatment group were significantly increased compared with the control group. …”
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Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride by Carlos Carpena-Torres, Jesús Pintor, María Jesús Pérez de Lara, Fernando Huete-Toral, Almudena Crooke, Cristina Pastrana, Gonzalo Carracedo

“…After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. …”
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E46K α-Synuclein Mutation Fails to Promote Neurite Outgrowth by Not Inducing Cdc42EP2 Expression, Unlike Wild-Type or A53T α-Synuclein in SK-N-SH Cells by Hyunja Jung, Seonghan Kim

“…Finally, to confirm the implication of neurite outgrowth, we knocked down Cdc42EP2 using small interfering RNA. Results: Unlike WT and A53T α-syn, E46K α-syn failed to promote neurite outgrowth by not inducing Cdc42EP2 and subsequent βIII-tubulin expression. …”
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Transcriptomic and Functional Landscape of Adult Human Spinal Cord NSPCs Compared to iPSC-Derived Neural Progenitor Cells by Sasi Kumar Jagadeesan, Ahmad Galuta, Ryan Vimukthi Sandarage, Eve Chung Tsai

“…In this study, we present the first direct transcriptomic and functional comparison of syngeneic adult human NSPC populations, including bona fide spinal cord NSPCs and iPSC-derived NSPCs regionalized to the spinal cord (iPSC-SC) and forebrain (iPSC-Br). RNA sequencing analysis revealed distinct transcriptomic profiles and functional disparities among NSPC types. iPSC-Br NSPCs exhibited a close resemblance to bona fide spinal cord NSPCs, characterized by enriched expression of neurogenesis, axon guidance, synaptic signaling, and voltage-gated calcium channel activity pathways. …”
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Nanostructured, Self-Assembling Peptide K5 Blocks TNF-α and PGE2 Production by Suppression of the AP-1/p38 Pathway by Woo Seok Yang, Yung Chul Park, Ji Hye Kim, Hye Ri Kim, Tao Yu, Se Eun Byeon, Larry D. Unsworth, Jaehwi Lee, Jae Youl Cho

“…Similarly, there was inhibition of cyclooxygenase- (COX-) 2 mRNA expression assessed by real-time PCR, indicating that the inhibition is at the transcriptional level. …”
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Investigating the Expression of c-Myc and BIRC5 in TRAIL and PBOX-15 treated Metastatic Prostate Cancer Cells by Corinne Kai Leng Chew, Hannah O'Toole, Daniela M Zisterer, Seema M. Nathwani, Jade K. Pollock

“…This study examined the effect of TRAIL, PBOX-15 and the combination on the target genes in prostate cancer cells. Methods RNA was extracted from treated LNCaP cells. cDNA was synthesized, and gene specific primers were subsequently used for amplification in a polymerase chain reaction (PCR) assay. …”
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miR-375 Promotes Pancreatic Differentiation In Vitro by Affecting Different Target Genes at Different Stages by Zhenyu Lu, Jing Wang, Xu Wang, Zhiying Li, Dan Niu, Min Wang, Jinzhu Xiang, Yongli Yue, Yajuan Xia, Xueling Li

“…Then, we performed overexpression and inhibition experiments of miR-375 on cells at different stages of differentiation and performed RNA-seq. The results showed that the expression of miR-375 fluctuated during hESC differentiation and was affected by miR-375 mimics and inhibitors. miR-375 influences global gene expression and the target genes of miR-375. …”
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Phytoalexin deficient 4 is associated with the lesion mimic trait in watermelon clalm mutant (Citrullus lanatus) by Jiale Shi, Congji Yang, Yuanyuan Qin, Qingqing Liu, Shengqi Hua, Defeng Wu, Wei Dong

“…Overexpression the ClCG04G001930 gene, designated as ClPAD4, yielded transgenic lines whose leaves gradually developed chlorotic lesions over 3 weeks. RNA interference of the ClPAD4 yielded transgenic lines whose cotyledon prone to diseased over 2 weeks. …”
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Lack of evidence for Mycoplasma spp. in bulk tank milk of herds located in mid-western Colombia by J. Velasco-Bolaños, A. S. Jaramillo-Jaramillo, N. A. Villa-Arcila, S. Piepers, S. Dufour, A. Ceballos-Márquez

“…Los análisis moleculares se realizaron por PCR multiplex usando cebadores específicos para los genes 16S-23S rRNA del Mycoplasma spp. y de algunas bacterias oportunistas ocasionales en la leche. …”
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A mouse model of immunosuppression facilitates oral Candida albicans biofilms, bacterial dysbiosis and dissemination of infection by Raja Veerapandian, Anuja Paudyal, Sarah M. Schneider, Sonny T. M. Lee, Govindsamy Vediyappan, Govindsamy Vediyappan

“…Intriguingly, we found co-occurrence of Enterococcus faecalis in the tongue, liver, and stool of immunosuppressed control and C. albicans infected organs. Targeted 16S rRNA and ITS2 amplicon sequencing of microbes from the fecal samples of mice confirmed the above results in the stool samples and revealed an inverse correlation of beneficial microbes in the dysbiosis condition. …”
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A universal DNA microarray for rapid fish species authentication by Patrizia Bade, Sebastian Stix, Kristina Kappel, Jan Fritsche, Ilka Haase, Andrew Torda, Nils Wax, Markus Fischer, Dirk Brandis, Ute Schröder

“…A universal set of 100 DNA probes (based on the genetic marker genes 16S ribosomal RNA and cytochrome b) generated species-specific DNA probe patterns for all 86 analyzed fish specimens. …”
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Resetting the Hsc70-mediated lysosomal degradation of PD-L1 via a supramolecular meso peptide for the restoration of acquired anti-tumor T cell immunity by Yujia Zhao, Dan Liu, Wenguang Yang, Wangxiao He, Jin Yan, Leiqing Yao

“…Furthermore, based on bioinformatics analysis of mRNA expression data from 30 types of tumors obtained from TCGA database, malignant melanoma was identified as the most suitable indication for CTAC-PDL1 due to its specific characteristics of tumor immune. …”
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Platelet-rich fibrin and silver nano-particles loaded chitosan treatment for post- laminectomy epidural scar adhesions: in vivo rats study model by Samah Fouad, Awad Rizk, Esam Mosbah, Mostafa M. Nabeeh, Walaa Awadin, Ayman S. Elmezayyen, Ekramy Elmorsy, Adel Zaghloul

“…After a 30-days follow-up, macroscopic examination, histological studies, and mRNA evaluation for TGFβ-1and IL-6, were conducted. …”
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Integrated bioinformatics and experimental analysis of CHAF1B as a novel biomarker and immunotherapy target in LUAD by Wei Du, Xiao-Wei Wu, Qing-Feng Li, Bing-Yu Zhang, Jing Wu, Ya-Ping Xu, Xue Yi

“…We also predicted lncRNAs-miRNA-CHAF1B axis to explore the molecular mechanism of CHAF1B in LUAD. …”
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