Comprehensive guide for epigenetics and transcriptomics data quality control by Arianna Comendul, Frederique Ruf-Zamojski, Colby T. Ford, Pankaj Agarwal, Elena Zaslavsky, German Nudelman, Manoj Hariharan, Aliza Rubenstein, Hanna Pincas, Venugopalan D. Nair, Adam M. Michaleas, Philip D. Fremont-Smith, Darrell O. Ricke, Stuart C. Sealfon, Christopher W. Woods, Kajal T. Claypool, Rafael Jaimes, III

“…Improved signature discovery, including the identification of the agent and timing of exposure, has been enabled by advancements in assaying techniques to detect RNA expression, DNA base modifications, histone modifications, and chromatin accessibility. …”
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Gender-specific antibiotic-resistance patterns and characterization of bacterial flora of undergraduate students’ mobile phones and associated factors at a Nigerian university by Morufat Oluwatosin Olaitan, Akeem Ganiyu Rabiu, Zara Babagana Umara

“…Bacterial isolates (n = 6/21) that resisted antibiotics belonging to ≥ 3 different classes regarded as multidrug resistant (MDR) were subjected to 16 S rRNA sequencing and identified using the Basic Local Alignment Search Tool (BLAST) algorithm. …”
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Exosomal circPOLQ promotes macrophage M2 polarization via activating IL-10/STAT3 axis in a colorectal cancer model by Yang Liu, Chen Chen, Jinbo Liu, Zhenqiang Sun, Bo Shao, Yanxin Xu, Pengyuan Dang, Shengyun Hu, Haifeng Sun, Chaoguan Wang, Junhong Hu

“…The mechanism underlying the effect of tumor-derived exosomes on M2 macrophage polarization remains elusive.Results The novel circular RNA circPOLQ exhibited significantly higher expression in CRC tissues than in paired normal tissues. …”
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Bacteria-Inspired Synthesis of Silver-Doped Zinc Oxide Nanocomposites: A Novel Synergistic Approach in Controlling Biofilm and Quorum-Sensing-Regulated Virulence Factors in <i>Pseu... by Abirami Karthikeyan, Manoj Kumar Thirugnanasambantham, Fazlurrahman Khan, Arun Kumar Mani

“…The potent isolated <i>Bacillus cereus</i> SASAK, identified via 16S rRNA sequencing and deposited in GenBank under accession number MH885570, facilitated the microbial-mediated synthesis of ZnO nanoparticles and silver-doped ZnO NCs. …”
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Sodium Lactate Accelerates M2 Macrophage Polarization and Improves Cardiac Function after Myocardial Infarction in Mice by Jialiang Zhang, Fangyang Huang, Li Chen, Guoyong Li, Wenhua Lei, Jiahao Zhao, Yanbiao Liao, Yijian Li, Changming Li, Mao Chen

“…Flow-cytometric analysis results reported that sodium lactate repressed the number of the IL-6+, IL-12+, and TNF-α+ macrophages among LPS-stimulated bone marrow-derived macrophages (BMDMs) and increased the mRNA levels of Arg-1, YM1, TGF-β, and IL-10. Mechanistic studies revealed that sodium lactate enhanced the expression of P-STAT3. …”
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Perspectives of using Illumina MiSeq for identification of arbuscular mycorrhizal fungi by A. A. Kryukov, A. O. Gorbunova, E. M. Machs, Y. V. Mikhaylova, A. V. Rodionov, P. M. Zhurbenko, A. P. Yurkov

“…In our work, we have successfully used universal primers ITS3 and ITS4 for the sequencing in Illumina MiSeq of the 5.8S rDNA – ITS2 region of the 35S rRNA genes, which contain both a conservative and variable regions. …”
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Antipsychotic Drugs Reverse MK801-Inhibited Cell Migration and F-actin Condensation by Modulating the Rho Signaling Pathway in B35 Cells by Yi-Chyan Chen, Fu-Ming Tsai, Mao-Liang Chen

“…MK801-induced psychotic symptoms and also the Ras homolog family member A (RhoA) expression and cell division control protein 42 (cdc42) mRNA modulation in the rat brain have been investigated. …”
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Faecalibacterium prausnitzii regulates carbohydrate metabolic functions of the gut microbiome in C57BL/6 mice by Peiling Geng, Ni Zhao, Yufan Zhou, Reuben S. Harris, Yong Ge

“…Multiomic approaches were used to profile the impact of UT1 on the gut microbiome and associated metabolic activity of C57BL/6 mice at homeostasis. Both 16S rRNA and metagenomic sequencing demonstrated that oral administration of UT1 resulted in profound microbial compositional changes including a significant enrichment of Lactobacillus, Bifidobacterium, and Turicibacter. …”
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Peptidoglycan-Like Components in Z-100, Extracted from Mycobacterium tuberculosis Strain Aoyama B, Increase IL-12p40 via NOD2 by Takayuki Horii, Yuki Orikawa, Yuta Ohira, Runa Eta, Uiko Tominaga, Takanori Sato, Takao Tanaka

“…We found that Z-100 contained peptidoglycan-like components. In addition, an siRNA study showed that Z-100 increased the production of IL-12p40 via nucleotide-binding oligomerization domain 2 (NOD2). …”
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miR-145 Alleviates Smooth Muscle Cell Phenotype Transition via ADAM17-Mediated ACE2 Shedding by Juan Wen, Baiyi Tang, Lan Guo, Wei Chen, Xiaohong Tang

“…Cotreatment with ADAM17 siRNA partially reversed the inducible effect of miR-145 inhibitor on the EREG and MMP2, induced Ang-(1–7) excretion, and upregulated ACE2 and Mas receptor expression. …”
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CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1 by Kamilla Wiull, Lisa K. Haugen, Vincent G. H. Eijsink, Geir Mathiesen

“…Importantly, the plasmid carrying the SgRNA, Cas9, and homology-directed repair template is designed for easy component exchange. …”
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Expansion of Circulating T Follicular Helper Cells in Children with Acute Henoch-Schönlein Purpura by Jue Xie, Yan Liu, Lei Wang, Guoxiang Ruan, Huiming Yuan, Hong Fang, Jianyong Wu, Dawei Cui

“…A positive correlation was observed between the frequencies of circulating ICOS+CXCR5+CD4+TFH cells and the serum IL-21 or IgA levels of acute HSP children, respectively. Additionally, the mRNA expression levels of interleukin- (IL-) 21, IL-6, and transcriptional factors (B-cell lymphoma-6, Bcl-6) were also significantly increased in peripheral blood from acute HSP children compared to HCs. …”
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Current molecular understanding of central nervous system schwannomas by Takahiro Tsuchiya, Satoru Miyawaki, Yu Teranishi, Kenta Ohara, Yudai Hirano, Shotaro Ogawa, Seiei Torazawa, Yu Sakai, Hiroki Hongo, Hideaki Ono, Nobuhito Saito

“…In addition to genetic alterations, research is also being conducted on gene expression and epigenetic regulation, with a focus on NF2 methylation and post-transcriptional silencing by micro RNA. Furthermore, the advent of advanced techniques like single-cell sequencing and multi-omics analysis has facilitated rapid discoveries related to the tumor microenvironment and tumor heterogeneity in schwannomas. …”
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The study of organelle DNA variability in alloplasmic barley lines in the NGS era by M. G. Siniauskaya, A. M. Makarevich, I. M. Goloenko, V. S. Pankratov, A. D. Liaudanski, N. G. Danilenko, N. V. Lukhanina, A. M. Shimkevich, O. G. Davydenko

“…Some of the SNPs detected are very similar in codon position and in the type of amino acid substitution to the places where RNA editing can occur. Thus, these results outline new perspectives for the future study of nuclear-cytoplasmic interactions in alloplasmic lines.…”
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N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells by Yanli Liu, Fen Yang, Shengying Liang, Qing Liu, Sulei Fu, Zhenyu Wang, Ciqing Yang, Juntang Lin

“…Moreover, upregulation of N-cadherin (N-cad) mRNA and protein expression was observed after neurogenic differentiation. …”
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Association of Serum Myeloid Cells of Soluble Triggering Receptor-1 Level with Myocardial Dysfunction in Patients with Severe Sepsis by Fei Tao, Liangshan Peng, Juan Li, Yiming Shao, Liehua Deng, Huaguo Yao

“…There is no difference in TREM-1-mRNA expression between the two groups. Conclusions. …”
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Composition of the sputum bacterial microbiome of patients with different pathomorphological forms of non-small-cell lung cancer by V. G. Druzhinin, E. D. Baranova, P. S. Demenkov, L. V. Matskova, A. V. Larionov

“…In the present study, we determined the taxonomic composition of the sputum microbiome of 52 patients with squamous cell carcinoma of the lung, 52 patients with lung adenocarcinoma and 52 healthy control donors, using next-generation sequencing (NGS) on the V3-V4 region of the bacterial gene encoding 16S rRNA. The sputum microbiomes of patients with different histological types of lung cancer and controls did not show significant differences in terms of the species richness index (Shannon); however, the patients differed from the controls in terms of evenness index (Pielou). …”
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Impact of Scleroderma‐Associated Autoantibodies on Clinical Outcome Assessments: Post Hoc Analysis From a Randomised, Double‐blind, Placebo‐controlled, Phase 3 Trial of Tocilizumab... by Basmah Al Dulaijan, Suiyuan Huang, Celia J. F. Lin, Christopher P. Denton, Dinesh Khanna

“…We analyzed four groups with SSc‐Abs: anti–topoisomerase 1 antibody (ATA), anti–RNA polymerase 3 antibody (RNAP3), anti‐centromere antibody, and negative for all three (triple negative). …”
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Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota by Kai Kang, Yue Sun, Dan Pan, Bing Chang, Li-Xuan Sang

“…Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment. Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment. …”
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Allicin Alleviates Inflammation of Trinitrobenzenesulfonic Acid-Induced Rats and Suppresses P38 and JNK Pathways in Caco-2 Cells by Chen Li, Weijian Lun, Xinmei Zhao, Shan Lei, Yandong Guo, Jiayi Ma, Fachao Zhi

“…Allicin reduced the body weight loss of trinitrobenzenesulfonic acid-induced rats, histological score, serum TNF-α and IL-1β levels, and colon IL-1β mRNA level and induced serum IL-4 level, particularly in combination with mesalazine. …”
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