Traffic-related-air-pollutant PM2.5 Caused Toxicity on Caenorhabditis elegans with Cotreatment of High-dose Glucose and Tempeh by Jian-He Lu, Ming-Hsien Tsai, Sen-Ting Huang, Jia-De Lee, Ta-Chih Hsiao, Wan Nurdiyana Wan Mansor, How-Ran Chao

“…In addition, tempeh treatment also ameliorated the altered mRNA expression of the antioxidant gene in C. elegans treated with or without TRAP PM2.5 and with or without high-dose glucose diets. …”
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Connexin 37 Regulates the Kv1.3 Pathway and Promotes the Development of Atherosclerosis by Minqi Liao, Lihua Chen, Jiongbin Lu, Guangzhu Liang, Yongzhao Yao, Shumin Ouyang, Yanhua Yang, Zhengwei Jian, Suxia Guo

“…The indexes of body weight, blood glucose, and blood lipid of mice were collected. The protein and mRNA expression levels of Cx37 and Kv1.3 were detected by reverse transcription-PCR (RT-PCR), Western blot, and immunofluorescence technique. …”
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Investigation of Scutellaria Barbata’s immunological mechanism against thyroid cancer using network pharmacology and experimental validation by Gen Ouyang, Yuangui Zhu, Zhehao Ouyang

“…Protein results indicated that quercetin downregulated SPP1 mRNA and protein expression. This study combines database predictions with experimental validation to reveal the potential mechanisms of SBH against TC, providing effective strategies for the immunotherapy of TC.…”
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Antiaging and Antioxidant Bioactivities of Asteraceae Plant Fractions on the Cellular Functions of the Yeast Schizosaccharomyces pombe by Rika Indri Astuti, Muhammad Eka Prastya, Irmanida Batubara, Eka Budiarti, Aulia Ilmiyawati

“…Interestingly, S. nodiflora water fractions significantly upregulated the mRNA transcripts of the Pap1-mediated core environmental stress response, namely, ctt1 gene in S. pombe. …”
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Sulforaphane enhances Nrf2-mediated antioxidant responses of skeletal muscle induced by exhaustive exercise in HIIT mice by Yangwenjie Wang, Yang Xiang, Ruiqi Wang, Xiangning Li, Jianxiong Wang, Siwang Yu, Ying Zhang

“…Importantly, SFN treatment improved antioxidative capacity of skeletal muscle in response to the acute exhaustive exercise by increasing mRNA and nucleoprotein expression of Nrf2 and these genes involved in antioxidant generation and decreasing blood creatine kinase (CK) and 4-hydroxy-2-nonenal (4-HNE)-modified protein levels in the HIIT mice.…”
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The Role of Yersinia enterocolitica O:3 Lipopolysaccharide in Collagen-Induced Arthritis by Katarzyna Kasperkiewicz, Anna S. Świerzko, Marta Przybyła, Janusz Szemraj, Jarosław Barski, Mikael Skurnik, Andrzej Kałużyński, Maciej Cedzyński

“…On the other hand, it seemed to be more hepatotoxic when compared with the other tested endotoxins, while the enlargement of inguinal lymph nodes and drop in hepatic MBL-C expression (at the mRNA level) were independent of LPS chemotype. Our data did not suggest no greater impact Y. enterocolitica O:3 on the development or severity of arthropathy related to anticollagen antibody-induced arthritis in mice, although its interaction with MBL-C and subsequent complement activation may contribute to some adverse effects.…”
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Molecular characterization and potential therapeutic roles of miR125a in HER-2 positive gastric cancer by Afraa Mamoori, Zena Hasan Sahib, Haider Alkafaji

“…In addition, the level of expression of this miRNA is correlated to different pathological and clinical parameters. …”
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Influenza B virus infection alters the regenerative potential of murine alveolar type 2 pneumocytes by Satoko Nakano, Cait E. Hamele, Aleksandra Tata, Purushothama Rao Tata, Nicholas S. Heaton

“…Here, the authors report that during an influenza B virus infection, some of the actively infected AT2 pneumocytes can ultimately eliminate all traces of the viral RNA and persist in the host long term. As a consequence of having been infected, however, the cells display an altered transcriptional profile and decreased proliferative capacity. …”
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miR-142-3p Contributes to Early Cardiac Fate Decision of Embryonic Stem Cells by Zhong-Yan Chen, Fei Chen, Nan Cao, Zhi-Wen Zhou, Huang-Tian Yang

“…Here, we report novel functions of miR-142-3p in the regulation of cardiomyocyte differentiation from mouse embryonic stem cells (mESCs). With a miRNA array screen, we identified a number of miRNAs significantly changed during mESC differentiation into the mesodermal and cardiac progenitor cells, and miR-142-3p was one among the markedly downregulated miRNAs. …”
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Hypoxia Inducible Factor-1α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α4 by Jong Ho Choi, Yun Bin Lee, Jieun Jung, Seong Gyu Hwang, IL-Hoan Oh, Gi Jin Kim

“…Knockdown of integrin α4 by integrin α4 siRNA (siITGA4) treatment increased BM-MSC migration by upregulation of ROCK1, Rac1/2/3, and matrix metalloproteinase-2 regardless of oxygen tension. …”
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EXO1 is a key gene for lung-resident memory T cells and has diagnostic and predictive values for lung adenocarcinoma by Zhuoqi Li, Xiaoyan Lin, Yuanhui Yang, Mei Tian, Lu Zhang, Fujing Huang, Xiao Wen, Zhigang Wei, Yuan Tian

“…However, the roles of EXO1 in the tumor microenvironment, and its application in the diagnosis and prognosis prediction of LUAD are still inadequately explored. In this study, the RNA expression, DNA methylation, CNV, somatic mutation data of EXO1, and the corresponding patients’ clinical information from publicly available databases were analyzed using bioinformatic methods. …”
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Genome-wide identification and expression analysis of ZF-HD family in sunflower (Helianthus annuus L.) under drought and salt stresses by Kangping Zhang, Ruqian Yan, Xiaoqing Feng, Jingjing Zhang, Yuanyuan Jiang, Lin Li, Jianzhong Guo, Xianping Zhang

“…In the tissue expression profile, HaZF-HD gene family had the highest expression in leaves and floral organs. Additionally, RNA-seq and RT-qPCR analysis revealed HaZF3 responded positively in the initial stage of drought stress, whereas a prompt and significant decrease of relative expression value in response to salt stress was showed in HaZF6 and HaZF9. …”
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Single-cell transcriptomics identify a novel macrophage population associated with bone invasion in pituitary neuroendocrine tumors by Xinzhi Wu, Xueshuai Han, Haibo Zhu, Mingxuan Li, Lei Gong, Sicheng Jing, Weiyan Xie, Zhaoqi Liu, Chuzhong Li, Yazhuo Zhang

“…Methods In this study, we performed single-cell (n = 87,287) RNA sequencing on 10 cases of bone-invasive PitNETs and 5 cases of non-bone-invasion PitNETs (Non-BI PitNETs). …”
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Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance by Ming Luo, Sitao Zhu, Hua Dang, Qing Wen, Ruixia Niu, Jiawei Long, Zhao Wang, Yongjia Tong, Yuese Ning, Meng Yuan, Guoyong Xu

“…Abstract Effective modulation of gene expression in plants is achievable through tools like CRISPR and RNA interference, yet methods for directly modifying endogenous proteins remain lacking. …”
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Metabolomics profiles revealed potential biomarkers of Pacific white shrimp (Litopenaeus vannamei) under cold and low salinity stress by Zhenzhu Sun, Pei Lin, Haobin Mai, Leling Chen, Zonglu Wei, Beiping Tan, Chaoxia Ye

“…LC-MS analysis revealed overlapping significant pathways, including ABC transporters, protein digestion, aminoacyl-tRNA biosynthesis, purine metabolism, and pyrimidine metabolism, with an additional pathway (mineral absorption) in the salinity change group. …”
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HMGB1 Acts in Synergy with Lipopolysaccharide in Activating Rheumatoid Synovial Fibroblasts via p38 MAPK and NF-κB Signaling Pathways by Zheng-Wen He, Yang-Hua Qin, Zhi-Wei Wang, Yan Chen, Qian Shen, Sheng-Ming Dai

“…HMGB1 alone failed to induce IL-6, MMP-3, and MMP-13 production in cultured SF but greatly enhanced LPS-induced expression of IL-6, MMP-3, and MMP-13 at both mRNA and protein levels. HMGB1-LPS synergistically upregulated TLR4 and receptor for advanced glycation endproducts (RAGE) expression on the surface of SF. …”
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Comparison of Two Field Deployable PCR Platforms for SARS-CoV-2 and Influenza A and B Viruses’ Detection by Hakim Bouamar, Grace M. Reed, Wanda Lyon, Hector Lopez, Anna Ochoa, Susana N. Asin

“…We compared the sensitivity and specificity of the Biomeme Franklin^TM and Truelab^® RT-PCR thermocyclers to determine which platform is more sensitive and specific at detecting SARS-CoV-2 and influenza A and B viruses. Methodology: RNA extracted from nasopharyngeal swabs of infected and uninfected individuals was tested on the Biomeme Franklin^TM at Lackland and the Truelab^® at Wright Patterson Air Force bases. …”
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Cell-Specific Regulation of Inflammatory Cytokines and Acute-Phase Proteins by the Glucocorticoid Receptor by Rebecca Winkler, Hong Lu

“…Primary human hepatocytes (PHH) were treated with combinations of tumor necrosis factor alpha (TNFα), lipopolysaccharide (LPS), and dexamethasone (DEX) for 24 h, following which chemokine mRNA and protein expression were analyzed using qPCR and enzyme-linked immunosorbent assay assays. …”
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Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands by Emily K. Bechtold, Jared B. Ellenbogen, Jorge A. Villa, Djennyfer K. de Melo Ferreira, Angela M. Oliverio, Joel E. Kostka, Virginia I. Rich, Ruth K. Varner, Sheel Bansal, Eric J. Ward, Gil Bohrer, Mikayla A. Borton, Kelly C. Wrighton, Michael J. Wilkins

“…To address this, we integrate 16S rRNA amplicon datasets, metagenomes, metatranscriptomes, and annual methane flux data across 9 wetlands, creating the Multi-Omics for Understanding Climate Change (MUCC) v2.0.0 database. …”
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Hypoxia inducible factor-dependent upregulation of Agrp in glomus type I cells of the carotid body by Luis Leon-Mercado, Ivan Menendez-Montes, Jonathan Tao, Bandy Chen, David P. Olson, C. Mackaaij, C.G.J. Cleypool, Laurent Gautron

“…In this study, utilizing histological and molecular biology techniques, we have identified a significant expression of Agrp mRNA and AgRP peptide production in glomus type I cells within the mouse carotid body (CB). …”
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