Testing the Purity of <i>Limnospira fusiformis</i> Cultures After Axenicity Treatments by Michael Schagerl, Alexander Kaptejna, Fabian Polz, Sameh S. Ali, Shuhao Huo, Joana Seneca, Petra Pjevac, Vera Hechtl

“…Contamination was also verified by cultivation-independent methods like flow cytometry and 16S rRNA genome amplicon sequencing. We detected taxa of the phyla Proteobacteria, Bacteriodota, Firmicutes and to a lesser extent Verrucomicrobiota. …”
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Odontogenic differentiation of dental pulp stem cells by glycogen synthase kinase-3β inhibitory peptides by Vidhyashree Rajasekar, Mohamed Mahmoud Abdalla, Mohammed S. Basbrain, Prasanna Neelakantan, Cynthia KY Yiu

“…The differentiation of hDPSCs following peptide treatment was determined using the alkaline phosphatase assay (ALP), calcium mineralization (alizarin red staining), and quantification of mRNA expression of differentiation markers via quantitative real-time polymerase chain reaction. …”
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The Role of Autophagy and Related MicroRNAs in Inflammatory Bowel Disease by Shiyuan Wang, Yan Huang, Cili Zhou, Huangan Wu, Jimeng Zhao, Luyi Wu, Min Zhao, Fang Zhang, Huirong Liu

“…Accumulating evidence demonstrates that microRNA- (miR-) mediated posttranscriptional regulation plays an important role in autophagy in inflammatory bowel disease (IBD), a disease that is difficult to manage clinically because of the associated chronic recurrent nonspecific inflammation. …”
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SIRT1/PGC-1α-mediated mitophagy participates the improvement roles of BMAL1 in podocytes injury in diabetic nephropathy: evidences from in vitro experiments by Yanxia Rui, Yinfeng Guo, Linying He, Min-er Wang, Henglan Wu

“…Results In HG-induced podocyte injury model, we found that BMAL1 and SIRT1 mRNA level was significantly decreased, and positively correlated with mitophagy dysfunction. …”
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Identification of the etiological agent of equine piroplasmosis in Western and Eastern Siberia by V. A. Rar, V. A. Marchenko, E. A. Efremova, O. V. Suntsova, O. V. Lisak, A. Y. Tikunov, I. V. Meltsov, N. V. Tikunova

“…The analysis of the determined 18S rRNA gene sequences demonstrated that T. equi samples belonged to two genetic groups, which differed significantly by the sequences of the variable (V4) region of the gene. …”
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Hyperlipidemia May Synergize with Hypomethylation in Establishing Trained Immunity and Promoting Inflammation in NASH and NAFLD by Charles I. V. Drummer, Fatma Saaoud, Yu Sun, Diana Atar, Keman Xu, Yifan Lu, Ying Shao, Candice Johnson, Lu Liu, Huimin Shen, Nirag C. Jhala, Xiaohua Jiang, Hong Wang, Xiaofeng Yang

“…We performed a panoramic analysis on both human nonalcoholic steatohepatitis (NASH) microarray data and microarray/RNA-seq data from various mouse models of nonalcoholic fatty liver disease NASH/NAFLD with total 4249 genes examined and made the following findings: (i) human NASH and NAFLD mouse models upregulate both cytokines and chemokines; (ii) pathway analysis indicated that human NASH can be classified into metabolic and immune NASH; methionine- and choline-deficient (MCD)+high-fat diet (HFD), glycine N-methyltransferase deficient (GNMT-KO), methionine adenosyltransferase 1A deficient (MAT1A-KO), and HFCD (high-fat-cholesterol diet) can be classified into inflammatory, SAM accumulation, cholesterol/mevalonate, and LXR/RXR-fatty acid β-oxidation NAFLD, respectively; (iii) canonical and noncanonical inflammasomes play differential roles in the pathogenesis of NASH/NAFLD; (iv) trained immunity (TI) enzymes are significantly upregulated in NASH/NAFLD; HFCD upregulates TI enzymes more than cytokines, chemokines, and inflammasome regulators; (v) the MCD+HFD is a model with the upregulation of proinflammatory cytokines and canonical and noncanonical inflammasomes; however, the HFCD is a model with upregulation of TI enzymes and lipid peroxidation enzymes; and (vi) caspase-11 and caspase-1 act as upstream master regulators, which partially upregulate the expressions of cytokines, chemokines, canonical and noncanonical inflammasome pathway regulators, TI enzymes, and lipid peroxidation enzymes. …”
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Carbon monoxide-oxidising Pseudomonadota on volcanic deposits by Robin A. Dawson, Nicola Fantom, Tamara Martin-Pozas, Patricia Aguila, Gary M. King, Marcela Hernández

“…CV2 (isolated from the 1917 soil). 16S rRNA gene sequencing showed that within the family Burkholderiacea, the genus Paraburkholderia dominated the 2015 volcanic deposit with an average relative abundance of 73.81%, whereas in the 1917 volcanic deposit, Cupriavidus accounted for 33.64% (average relative abundance). …”
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IL-10 and ARG-1 Concentrations in Bone Marrow and Peripheral Blood of Metastatic Neuroblastoma Patients Do Not Associate with Clinical Outcome by Fabio Morandi, Michela Croce, Giuliana Cangemi, Sebastiano Barco, Valentina Rigo, Barbara Carlini, Loredana Amoroso, Vito Pistoia, Silvano Ferrini, Maria Valeria Corrias

“…In both BM and PB samples, IL-10 mRNA expression was higher in metastatic NB patients than in controls. …”
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Research progress of gut flora in improving human wellness by Chenggang Zhang, Wenjing Gong, Zhihui Li, Dawen Gao, Yan Gao

“…Therefore, “gut flora priority principle” is developed to guarantee human wellness. The 16S rRNA sequencing and mass spectrometric techniques can be used to identify the gut flora, which may guide us to a new era of human wellness based on gut flora wellness. …”
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High Levels of Proinflammatory Cytokines, but Not Markers of Tissue Injury, in Unaffected Intestinal Areas from Patients with IBD by Alberto J. León, Emma Gómez, Jose A. Garrote, David Bernardo, Asterio Barrera, Jose L. Marcos, Luis Fernández-Salazar, Benito Velayos, Alfredo Blanco-Quirós, Eduardo Arranz

“…In general, a correlation was observed between mRNA levels of these cytokines and both iNOS and Granzyme B. …”
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Betaine enhances SCAPs chondrogenic differentiation and promotes cartilage repair in TMJOA through WDR81 by Meiyue Wang, Zejie Wu, Xiaoyu Zheng, Yishu Huang, Yizhou Jin, Jiaxin Song, Wanzhen Lei, Hua Liu, Riyue Yu, Haoqing Yang, Runtao Gao

“…In vivo cartilage regeneration effects were evaluated by the rat TMJOA model. RNA-sequencing and biological analyses were performed to select target genes and biological processes involved. …”
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Intestinal microbiota is altered in patients with colon cancer and modified by probiotic intervention by Ashley A Hibberd, Anna Lyra, Arthur C Ouwehand, Peter Rolny, Helena Lindegren, Lennart Cedgård, Yvonne Wettergren

“…The colonic and faecal microbiota was assessed by 16S rRNA gene amplicon sequencing.Results The tumour microbiota was characterised by increased microbial diversity and enrichment of several taxa including Fusobacterium, Selenomonas and Peptostreptococcus compared with the control microbiota. …”
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Stevenleaf from Gynostemma Pentaphyllum inhibits human hepatoma cell (HepG2) through cell cycle arrest and apoptotic induction by Sayed Sajid Hussain, Fan Zhang, Yuanyuan Zhang, Kiran Thakur, Mahrukh Naudhani, Carlos L. Cespedes-Acuña, Zhaojun Wei

“…For cell cycle arrest, the mRNA expression levels of p21, p27 and p53 were up-regulated, while the expression levels of Cyclin A, Cyclin D1, Cyclin E and CDK1/2 were down-regulated. …”
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Polyphenols synergistic drugs to ameliorate non-alcoholic fatty liver disease via signal pathway and gut microbiota: A review by Hongcai Li, Jingjing Liang, Mengzhen Han, Zhenpeng Gao

“…Furthermore, some emerging mechanisms such as microRNA potential biomarker therapies may provide therapeutic avenues for NAFLD. …”
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Vitrification affects the post-implantation development of mouse embryos by inducing DNA damage and epigenetic modifications by Yurong Chen, Haibo Zhu, Fucheng Guo, Luyao Wang, Wenli Zhang, Ruizhi Liu, Xiaoling Zhang, Xiangpeng Dai

“…The related gene expression and transcriptome profiles were investigated by RT-qPCR and RNA-seq, respectively. Blastocyst and implantation frequencies were not significantly affected by vitrification. …”
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DNA Methylation Is Involved in the Expression of miR-142-3p in Fibroblasts and Induced Pluripotent Stem Cells by Siti Razila Abdul Razak, Yukihiro Baba, Hiromitsu Nakauchi, Makoto Otsu, Sumiko Watanabe

“…We have been analyzing comprehensive expression patterns of microRNA in human and mouse embryonic stem and induced pluripotent stem cells. …”
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Impact of Age and Sex on Viral Load in Hepatitis C Virus Infection by Andrea Magri, Giulia Francesca Manfredi, Carlo Smirne, Silvia Pigni, Michela Emma Burlone, Mattia Bellan, Nicole Vercellino, Rosalba Minisini, Mario Pirisi

“…Females were older (median age 68 vs. 57, <i>p</i> < 0.001) and had a higher prevalence of genotype 2 (33% vs. 20%, <i>p</i> < 0.001) than males; there was no difference between sexes regarding the METAVIR stage. The median HCV RNA concentration was 1.017 × 10⁶ IU/mL (interquartile range, 0.286–2.400). …”
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Kruppel-family zinc finger proteins as emerging epigenetic biomarkers in head and neck squamous cell carcinoma by Patrick Pearson, Kendra Smith, Nilita Sood, Elizabeth Chia, Alicia Follett, Michael B. Prystowsky, Simon Kirby, Thomas J. Belbin

“…Results In 20 patients from the TCGA cohort of HNSCC patients where ZNF154 and ZNF132 DNA methylation and RNA expression could be compared in tumor and adjacent normal tissue, there was increased DNA methylation and decreased expression of both ZNF154 and ZNF132 in primary tumours. …”
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Exploring serum miR-33b as a novel diagnostic marker for hypercholesterolemia and obesity: insights from a pilot case-control study by Yaser Masoumi-Ardakani, Mostafa Eghbalian, Hossein Fallah, Alireza Jafari, Beydolah Shahouzehi

“…MicroRNAs (miRNAs) are small, conserved, non-coding RNA sequences consisting of approximately 22 nucleotides, playing crucial roles in biological and pathological functions. …”
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Sox9-Increased miR-322-5p Facilitates BMP2-Induced Chondrogenic Differentiation by Targeting Smad7 in Mesenchymal Stem Cells by Yongsheng Zeng, Chengcheng Du, Pengcheng Xiao, Yiting Lei, Piao Zhao, Zhenglin Zhu, Shengqiang Gao, Bowen Chen, Shengwen Cheng, Wei Huang, Chen Zhao

“…The purpose of this study was to determine which microRNA is increased by Sox9 and targets Smad7, thus assisting BMP2 in maintaining stable chondrogenesis. …”
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