Synteza celulaz w hodowlach bakterii Paenibacillus sp. wyizolowanych z ryzosfery by Jakub Dobrzyński, Monika Sitarek, Patrycja Słodownik, Urszula Jankiewicz, Dariusz Gozdowski, Ewa B. Górska

“…Na podstawie morfologii, cech biochemicznych i sekwencji 16S rRNA izolaty sklasyfikowano do gatunków z rodzaju Paenibacillus. …”
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Intratumor Genetic Heterogeneity of Breast Carcinomas as Determined by Fine Needle Aspiration and TaqMan Low Density Array by Maria B. Lyng, Anne-Vibeke Lænkholm, Niels Pallisgaard, Werner Vach, Ann Knoop, Martin Bak, Henrik J. Ditzel

“…A tissue biopsy of the tumors was also snap-frozen for comparison. Non-amplified RNA was investigated by the novel qRT-PCR-based technique, Low Density Array (LDA) using 4 reference genes and 44 target genes. …”
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Studying the impact of chitosan salicylaldehyde/schiff base/CuFe2O4 in PC3 cells via theoretical studies and inhibition of PI3K/AKT/mTOR signalling by Ghada H. Elsayed, Asmaa M. Fahim

“…After 48 h, PC3 cell proliferation was significantly inhibited by chitosan salicylaldehyde/CuFe2O4 and chitosan salicylaldehyde (IC50 = 35.3 and 45.55 µg/ml, respectively) without any effects on normal HSF cells. The mRNA expression levels of PI3K, AKT, mTOR, and CCND1 were examined in PC3-treated cells by using QRT-PCR, and the results demonstrated that, by down-regulating the expression levels of these genes, chitosan salicylaldehyde/CuFe2O4 significantly affected prostate cancer cell proliferation, progression, and autophagy more than chitosan salicylaldehyde. …”
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Serum Levels of Mitochondrial Fission- and Fusion-Related Genes of Coal Workers’ Pneumoconiosis and Risk Factor Analysis Based on a Generalized Linear Model by Xiao-Yu Li, Jing-Lin Wei, Yong-Xin Xie, Ji Zhao, Li-Ya Ma, Na Zhang, Hui-Fang Yang

“…Blood samples were collected to detect the ROS content, MDA content, and SOD activity. The mRNA expression levels of OPA1, Drp1, MFN2, Fis1, Col I, Col III, and α-SMA were determined by q-PCR. …”
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The EGF Receptor and HER2 Participate in TNF-α-Dependent MAPK Activation and IL-8 Secretion in Intestinal Epithelial Cells by Humberto B. Jijon, Andre Buret, Christina L. Hirota, Morley D. Hollenberg, Paul L. Beck

“…Downregulation of HER2 via siRNA resulted in a significant decrease in ERK phosphorylation and a 50% reduction in IL-8 secretion.…”
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Multi-omics analysis reveals the interplay between intratumoral bacteria and glioma by Ting Li, Zhanyi Zhao, Meichang Peng, Lu Zhang, Cheng Wang, Feiyang Luo, Meiqin Zeng, Kaijian Sun, Zhencheng Fang, Yunhao Luo, Yugu Xie, Cui Lv, Jiaxuan Wang, Jian-Dong Huang, Hongwei Zhou, Haitao Sun

“…Importantly, we found bacterial RNA and LPS signals within glioma tissues, which were traditionally considered sterile. …”
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Hepatitis D virus infection triggers CXCL9-11 upregulation in hepatocytes and liver infiltration of CXCR3+ CD4 T cells by Jan-Hendrik Bockmann, Lena Allweiss, Annika Volmari, David da Fonseca Araújo, Matin Kohsar, Anastasia Hyrina, Janine Kah, Zhijuan Song, Josolyn Chan, Katja Giersch, Tassilo Volz, Marc Lütgehetmann, Jeffrey J. Wallin, Dmitry Manuilov, Meghan M. Holdorf, Simon P. Fletcher, Ansgar W. Lohse, Antonio Bertoletti, Julian Schulze zur Wiesch, Maura Dandri

“…Similarly, high levels of CXCL9–11 were observed in HDV-infected PHHs in vitro. Analysis by RNA in situ hybridisation on patient livers revealed that HDV-infected hepatocytes were a significant contributor to the chemokine expression. …”
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miR-317 regulates the proliferation and apoptosis of duck follicle granulosa cells by targeting VIPR1 by Li Li, Qingwu Xin, Linli Zhang, Zhongwei Miao, Zhiming Zhu, Xiaopan Liu, Qiannan Cai, Wenli Shi, Bangzhe Zhao, Changtao Liu, Zhengkui Zhou, Azheng Liang, Qinlou Huang, Nenzhu Zheng

“…More than 90 % of the cells were identified as duck follicle granulosa cells by immunofluorescence staining of FSHR and LHR. miR-317 expression was significantly higher in the miR-317 mimic-transfected group than in the miRNA-NC-transfected group (P < 0.01); similarly, miR-317 expression was significantly lower in the inhibitor-transfected group than in the miRNA inhibitor-transfected group (P < 0.01), indicating that miR-317 overexpression and interference vectors were successfully constructed and transfected into duck follicular granulosa cells. …”
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Basic Molecular Biology, Metabolic and Immunological Mechanisms of Fecal Microbiota Transplantation by O. V. Goloshchapov, A. V. Beynarovich, A. B. Chukhlovin, R. V. Klementeva, A. A. Shcherbakov, E. A. Bakin, I. K. Golubovskaya, N. D. Ventslovayte, O. S. Yudintseva, M. V. Gorchakova, V. E. Karev, M. A. Kucher, M. A. Suvorova, E. V. Svetkina, G. G. Rodionov, V. V. Gostev, S. V. Sidorenko, I. S. Moiseev, A. D. Kulagin

“…To monitor the basic effects of FMT, we performed targeted DNA sequencing of 16S rRNA gene (V3–V4) using MiSeq platform as well as multiplex real-time PCR, MS/gas chromatography technique, immunophenotyping of blood lymphocytes, histological and immunohistochemical techniques.Clinical case. …”
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Effect of ectoparasite Ichthyophthirius multifiliis on the histopathology and gill and gut microbiota of goldfish (Carassius auratus) by Xialian Bu, Xialian Bu, Xianqi Peng, Xianqi Peng, Lei Huang, Lei Huang, Yu Zhao, Jinbiao Jiao, Jinbiao Jiao, Jian Zhu, Jing Chen, Jing Chen, Xiaohong Huang, Xiaohong Huang, Aqin Zheng, Aqin Zheng, Huantao Qu, Jiayun Yao, Jiayun Yao

“…However, whether it influences gill and gut microbiota is still unknow.MethodsIn this study, H&amp;E staining was used to show the gill and gut histopathological characteristics of I. multifiliis-infected and uninfected goldfish (Carassius auratus). Meanwhile, 16S rRNA gene amplicon sequencing was conducted to analyze the difference of gill and gut microbiota between I. multifiliis-infected and uninfected goldfish.ResultsHistopathological examination revealed that I. multifiliis has induced significant damage to the gills of goldfish, characterized by lamellae fusion, cell hyperplasia, cell hyperaemia, inflammatory infiltration, necrosis and desquamation. 16S rRNA gene sequencing result showed that alpha and beta diversity of gill microbiota was significantly reduced in the I. multifiliis-infected group, while no significant changes were observed in gut microbiota. …”
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Clinical-grade extracellular vesicles derived from umbilical cord mesenchymal stromal cells: preclinical development and first-in-human intra-articular validation as therapeutics f... by Aliosha I. Figueroa-Valdés, Patricia Luz-Crawford, Yeimi Herrera-Luna, Nicolás Georges-Calderón, Cynthia García, Hugo E. Tobar, María Jesús Araya, José Matas, Darío Donoso-Meneses, Catalina de la Fuente, Jimena Cuenca, Eliseo Parra, Fernando Lillo, Cristóbal Varela, María Ignacia Cádiz, Rolando Vernal, Alexander Ortloff, Gino Nardocci, Verónica Castañeda, Catalina Adasme-Vidal, Maximiliano Kunze-Küllmer, Yessia Hidalgo, Francisco Espinoza, Maroun Khoury, Francisca Alcayaga-Miranda

“…UC-MSC-sEV characterization under non-cGMP conditions showed consistent miRNA and protein profiles, suggesting their potential for standardized manufacturing. …”
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Serum Levels and Placental Expression of NGAL in Gestational Diabetes Mellitus by Xiaoqian Yin, Yan Huo, Li Liu, Yixing Pan, Suxin Liu, Runfang Wang

“…In the GDM group, maternal serum NGAL levels exhibited a positive correlation with placental NGAL mRNA and protein levels (r = 0.848, P=0.008; r = 0.636, P=0.011, respectively). …”
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Inflamm-Aging-Related Cytokines of IL-17 and IFN-γ Accelerate Osteoclastogenesis and Periodontal Destruction by Jingyi Tan, Anna Dai, Lai Pan, Lan Zhang, Zhongxiu Wang, Ting Ke, Weilian Sun, Yanmin Wu, Pei-Hui Ding, Lili Chen

“…For receptor activator of nuclear factor-κB ligand- (RANKL-) primed bone marrow macrophages (BMMs), IL-17 and IFN-γ enhanced osteoclastogenesis, with the expression of osteoclastogenic mRNA (TRAP, c-Fos, MMP-9, Ctsk, and NFATc1) and protein (c-Fos and MMP-9) upregulated. …”
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Pirfenidone Inhibits Hypoxic Pulmonary Hypertension through the NADPH/ROS/p38 Pathway in Adventitial Fibroblasts in the Pulmonary Artery by Song Zhang, ZongXiu Yin, WeiDong Qin, XiaoLi Ma, Yao Zhang, EnXiu Liu, YanBiao Chu

“…NOX4 and Rac1 were inhibited or overexpressed by shRNA or pcDNA3.1, respectively. Proliferation of HPAAFs was quantified by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assays to assess cellular metabolic activity, cell counts, and ethynyldeoxyuridine (EdU) assays to detect DNA synthesis. …”
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Distinctive Gut Microbiota Alteration Is Associated with Poststroke Functional Recovery: Results from a Prospective Cohort Study by Yini Dang, Xintong Zhang, Yu Zheng, Binbin Yu, Dijia Pan, Xiaomin Jiang, Chengjie Yan, Qiuyu Yu, Xiao Lu

“…Their fecal DNAs were extracted, and the V3-V4 regions of the conserved bacterial 16S RNA were amplified and sequenced on the Illumina MiSeq platform. …”
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Anthracophyllum sontraense (Omphalotaceae, Agaricales), A New Species from Danang, Vietnam by Trung Thanh Nguyen, Hanh Hong Do, Toan Nguyen-Sy, Uyen Thi Diem Huynh, Nguyen Thi Lam Hai, Thi Thanh Tam Phan, Thi Thu Hoai Nguyen, Do Tan Khang, Phu Thi Tran

“…The phylogenetic analyses based on the combined internal transcribed spacer (ITS) and Large Subunit (LSU) molecular markers of ribosomal RNA genes revealed that the two specimens clustered in a single clade at species level with all tested Anthracophyllum species with strong bootstrap support of 99%. …”
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Gene Expression Differences Predict Treatment Outcome of Merkel Cell Carcinoma Patients by Loren Masterson, Bryan J. Thibodeau, Laura E. Fortier, Timothy J. Geddes, Barbara L. Pruetz, Rajwant Malhotra, Richard Keidan, George D. Wilson

“…Laser capture microdissection was used to isolate tumor cells for subsequent RNA isolation and gene expression analysis with Affymetrix GeneChip Human Exon 1.0 ST arrays. …”
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Advancements in Managing Anthracycline-Induced Cardiotoxicity: Insights from Interventional Clinical Trials by Zhao M, Zhang X, Zhou D, Song J

“…Advanced monitoring techniques, including global longitudinal strain (GLS)-guided echocardiography, cardiac magnetic resonance (CMR) and novel biomarkers (cfDNA, microRNA), showed promise in early AIC detection. Exercise interventions demonstrated significant cardiovascular benefits.Conclusion: Cardioprotective agents, early detection methods, and exercise interventions are key to managing AIC. …”
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Gut microbial alteration in chronic spontaneous urticaria unresponsive to second generation antihistamines and its correlation with disease characteristics‐ a cross‐sectional case‐... by Indrashis Podder, David Pesqué, Nerea Carrón, Pedro Iñaki González Torres, Ramon M. Pujol, Ana M. Giménez‐Arnau

“…Clinico‐demographic profile, laboratory investigations and stool analysis were conducted in all study participants. 16S RNA gene sequencing and DNA isolation was performed for all stool samples, followed by bioinformatic analysis. …”
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Five New Indole Alkaloid Derivatives from Deep-Sea Fungus <i>Aspergillus fumigatus</i> AF1 by Lai-Hui Dai, Gao-Rong Zhang, Yang-Hui Ou, Xiao-Jing Liu, Hong-Liang Yao, Wen-Hao Hu, Hou-Jin Li, Wen-Jian Lan

“…Compounds <b>8</b> and <b>13</b> displayed significant downregulation of the mRNA expression levels of all three molecular markers (COL1A1, α-SMA and FN1), with compound <b>13</b> exhibiting the best performance among all the tested compounds.…”
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