PPARD May Play a Protective Role for Major Depressive Disorder by Tao Yang, Juhua Li, Liyuan Li, Xuehua Huang, Jiajun Xu, Xia Huang, Lijuan Huang, Kamil Can Kural

“…Then, we tested the PPARD expression changes in MDD patients from 18 independent MDD RNA expression datasets, followed by coexpression analysis, multiple linear regression analysis, and a heterogeneity analysis to study the influential factors for PPARD expression levels. …”
Get full text

Adiponectin Deficiency Blunts Hypoxia-Induced Mobilization and Homing of Circulating Angiogenic Cells by Bert R. Everaert, Vincent J. Nijenhuis, Florence C. M. Reith, Vicky Y. Hoymans, Jean-Pierre Timmermans, Christiaan J. Vrints

“…One week after AMI, bone marrow (BM) concentration and mobilization of Sca-1+ and Lin−Sca-1+ progenitor cells (PCs) were markedly attenuated under Adipoq−/− conditions, as assessed by flow cytometry. The mRNA expression of HIF-1-dependent chemotactic factors, such as Cxcl12 (P=0.005) and Ccl5 (P=0.025), and vascular adhesion molecules, such as Icam1 (P=0.010), and Vcam1 (P=0.014), was significantly lower in the infarction border zone of Adipoq−/− mice. …”
Get full text

Comparison of Bacterial Community Structure and Diversity in Traditional Gold Mining Waste Disposal Site and Rice Field by Using a Metabarcoding Approach by Fatimawali, Billy Johnson Kepel, Maria Apriliani Gani, Trina Ekawati Tallei

“…Analysis of bacterial communities and structure was carried out based on the results of metabarcoding of the V3-V4 16S rRNA regions obtained from paired-end Illumina MiSeq reads. …”
Get full text

The role of MRO as an M2 macrophage-associated gene in non-small cell lung cancer: insights into immune infiltration, prognostic significance, and therapeutic implications by Yue Gu, Miaosen Zheng, Jing Xie

“…Univariate Cox regression and Kaplan–Meier analyses assessed prognostic significance. Single-cell RNA sequencing data from TISCH2 evaluated MRO expression in different cell types. …”
Get full text

Molecular Analysis of the Bacterial Communities in Crude Oil Samples from Two Brazilian Offshore Petroleum Platforms by Elisa Korenblum, Diogo Bastos Souza, Monica Penna, Lucy Seldin

“…Crude oil samples with high- and low-water content from two offshore platforms (PA and PB) in Campos Basin, Brazil, were assessed for bacterial communities by 16S rRNA gene-based clone libraries. RDP Classifier was used to analyze a total of 156 clones within four libraries obtained from two platforms. …”
Get full text

Antimicrobial peptide AP2 ameliorates Salmonella Typhimurium infection by modulating gut microbiota by Lianglan Li, Qiufen Mo, Yi Wan, Yuanhao Zhou, Weiqin Li, Weifen Li

“…These effects were evidenced by reduced ST-induced body weight loss and lower levels of serum inflammatory cytokines. A 16 S rRNA-based analysis of the cecal microbiota revealed that AP2 significantly modulated the gut microbiota, increasing the relative abundance of Bifidobacterium while decreasing that of Akkermansia at the genus level. …”
Get full text

ANGPTL4 Attenuates Ang II-Induced Atrial Fibrillation and Fibrosis in Mice via PPAR Pathway by Xi Zhu, Xiaogang Zhang, Xinpeng Cong, Luoning Zhu, Zhongping Ning

“…The fibrosis was evaluated with Masson’s trichrome staining in the atrial myocardium. mRNA levels of IL-1β, IL-6, collagen I, and collagen III were measured using real-time qRT-PCR. …”
Get full text

Transcriptomic data reveals MYC as an upstream regulator in laying hen follicular recruitment by Ashley E Kramer, Kathryn M Ellwood, Nicole Guarino, Cong-Jun Li, Aditya Dutta

“…This study investigates the genetic drivers mediating the transition of pre-recruitment follicles to the pre-ovulatory phase, a crucial stage before ovulation. Using RNA sequencing and bioinformatics approaches such as a differential gene expression analysis, we compared pre-recruitment follicles with the recently recruited F5 pre-ovulatory follicle to identify key genes and upstream regulators involved in this transition. …”
Get full text

MRP8/ABCC11 Expression Is Regulated by Dexamethasone in Breast Cancer Cells and Is Associated to Progesterone Receptor Status in Breast Tumors by Mylène Honorat, Aurélia Mesnier, Julie Vendrell, Attilio Di Pietro, Valérie Lin, Charles Dumontet, Pascale Cohen, Léa Payen

“…The ATP-binding cassette multidrug resistance protein 8 (MRP8/ABCC11) mediates the excretion of anticancer drugs. ABCC11 mRNA and protein levels were enhanced by DEX (dexamethasone) and by PROG (progesterone) in MCF7 (progesterone receptor-(PR-) positive) but not in MDA-MB-231 (PR-negative) breast cancer cells. …”
Get full text

Cryo-thermal therapy reshaped the tumor immune microenvironment to enhance the efficacy of adoptive T cell therapy by Shicheng Wang, Peng Peng, Junjun Wang, Zelu Zhang, Ping Liu, Lisa X. Xu

“…The right large tumor was subjected to CTT, and the left small tumor was collected for flow cytometry, RNA-seq, immunohistochemistry and TCR Vβ sequencing. …”
Get full text

Mapping dynamic regulation of gene expression using single-cell transcriptomics and application to complex disease genetics by Hanna Abe, Phillip Lin, Dan Zhou, Douglas M. Ruderfer, Eric R. Gamazon

“…However, the identification of gene-level associations in distinct cell types faces several challenges, including the limited reference resources from population-scale studies, data sparsity in single-cell RNA sequencing, and the complex cell state pattern of expression within individual cell types. …”
Get full text

CRISPR/Cas genome editing for cotton precision breeding: mechanisms, advances, and prospects by Vijay Sheri, Harikrishnan Mohan, Phanikanth Jogam, Anshu Alok, Gulab Khan Rohela, Baohong Zhang

“…To ensure specificity and avoiding off-target effects, we need to optimize various parameters such as target site, guide RNA design, and choosing right Cas variants. We outline the future prospects of CRISPR/Cas in cotton breeding, suggesting areas for further research and innovation. …”
Get full text

TIM‐4 increases the proportion of CD4+CD25+FOXP3+ regulatory T cells in the pancreatic ductal adenocarcinoma microenvironment by inhibiting IL‐6 secretion by Ziyao Wang, Zerong Xie, Yu Mou, Ruiman Geng, Chen Chen, Nengwen Ke

“…The influence of TIM‐4 overexpression on cell function was analyzed using RNA‐seq. Flow cytometry and ELISA were used for verification. …”
Get full text

RAB3GAP2 dysregulation in adult T-cell leukemia/lymphoma (ATLL) compared to acute lymphoblastic leukemia (ALL): a molecular perspective by Samira Pourrezaei, Arash Letafati, Ghazale Molaverdi, Mehdi Norouzi, Sayed-Hamidreza Mozhgani

“…Real-time quantitative PCR was then performed to quantify mRNA expression levels of SMC6, FANCM, EIF4H, WDR7, RAB3GAP2, and IFN α/β. …”
Get full text

The role of HSPA14 in breast cancer: implications for tumorigenesis, immune response modulation, and personalized therapies by Ruipeng Zhao, Xiaocun Zhu, Wan Wei, Linlin Zhen

“…The data on HSPA14 levels and drug sensitivity were extracted from the CellMiner dataset. The mRNA expression of HSPA14 was validated through cell experiments.Results HSPA14 expression is elevated in breast cancer, which is associated with poor overall survival. …”
Get full text

Uptake of iron from ferrous fumarate can be mediated by clathrin-dependent endocytosis in Hutu-80 cells by Agata Tarczykowska, Per Malmberg, Nathalie Scheers

“…Here we propose that iron from the simple salt ferrous fumarate, a common iron supplement, can be absorbed by clathrin-mediated endocytosis. We used siRNA to silence DMT1 transporter expression, pharmacological inhibition of endocytosis, and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) to show that iron uptake from ferrous fumarate can be mediated by both transport via DMT1 and by clathrin-dependent endocytosis in Hutu-80 cells. …”
Get full text

PATHOPHYSIOLOGIC MECHANISMS OF THE INFLUENCE OF TISSUE RESPIRATION ENZYMES ON THE MITOCHONDRIAL FUNCTION IN PATIENTS WITH CHRONIC HEPATITIS C by I.Y. Bagmut, S.M. Gramatiuk

“…The HCV infection was diagnosed by the positivity of anti-HCV and HCV-RNA for at least 6 months of period. Mitochondrial integrity was assessed by cytochrome C release using a commercial kit (Cytochrome C Oxidase Assay Kit, Sigma-Aldrich, St. …”
Get full text

Mitochondrial ATP-Sensitive K+ Channel Opening Increased the Airway Smooth Muscle Cell Proliferation by Activating the PI3K/AKT Signaling Pathway in a Rat Model of Asthma by Min Gao, Qinran Sun, Qingfa Liu

“…The proliferation of ASMCs was examined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The protein and mRNA expressions of AKT and p-AKT were detected using western blotting and quantitative real-time PCR. …”
Get full text

Enhanced Blastocystis subtyping from stool samples using semi-nested barcode PCR: validation with an NGS-based approach by Carlos Nieto-Clavijo, Liliana Morales, Andrés Delgado-Aldana, Paula C. Hernández, Isabel Torres-Molina, Amanda Gonzalez-Cuiza, Fabián Cortés-Muñoz, Jacqueline Chaparro-Olaya

“…In 2006, a PCR method was introduced to subtype Blastocystis by Sanger sequencing of an ≈610 bp amplicon of the 18S rRNA gene. This method, known as barcoding-PCR, has become widespread, although the primer pair used can amplify non-Blastocystis sequences, which can result in false positives. …”
Get full text

Curcumin Attenuation of Wear Particle-Induced Osteolysis via RANKL Signaling Pathway Suppression in Mouse Calvarial Model by Tao Cheng, Yaochao Zhao, Bin Li, Mengqi Cheng, Jiaxing Wang, Xianlong Zhang

“…Furthermore, CUR downregulated the osteoclast-specific gene expression and reversed the receptor activator of nuclear factor kappa-B ligand (RANKL)/osteoprotegerin messenger RNA and protein ratio in PMMA particle-stimulated mice. …”
Get full text