The role of IFN-γ/CXCL10 axis in Mycoplasma pneumonia infection by Yanxia Zou, Feng Huang, Jiying Sun, Yidan Zheng, Ge Dai, Ting Wang, Canhong Zhu, Yongdong Yan, Renzheng Wang, Zhengrong Chen

“…In vitro, coculture with IFN-γ or activated CD4+ Th1 cells significantly promoted CXCL10 expressions in THP-1 derived macrophages, which was largely reversed by siRNA-mediated down regulation of STAT1. In addition, IFN-γ-stimulated macrophages greatly promoted the trans-migration of Th1 cells. our data show that Th1 cells-derived IFN-γ augments CXCL10 production in macrophages via the JAK-STAT1 pathway, which subsequently recruits more immune cells like Th1 cells into the infection sites, thereby constituting a positive feedback loop and aggravating the type I inflammatory responses in MPP patients.…”
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Mettl3-m6A-NPY axis governing neuron–microglia interaction regulates sleep amount of mice by Qihang Sun, Jinpiao Zhu, Xingsen Zhao, Xiaoli Huang, Wenzheng Qu, Xia Tang, Daqing Ma, Qiang Shu, Xuekun Li

“…In the present study, we showed that the deficiency of RNA m6A methyltransferase Mettl3 in excitatory neurons specifically induces microglia activation, neuroinflammation and neuronal loss in thalamus of mice. …”
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Chitosan Modulates Inflammatory Responses in Rats Infected with Enterotoxigenic Escherichia coli by Gang Liu, Shuai Chen, Guiping Guan, Jun Tan, Naif A. Al-Dhabi, Hongbing Wang, Veeramuthu Duraipandiyan, Jun Fang

“…At day 7 after infection, the bacterial load in the jejunum and faeces was significantly lower in the COS group than that in the control group. Moreover, the mRNA and protein levels of IL-1β, IL-6, IL-17, IL-18, and TNF-α were significantly lower in the group of mice receiving the COS diet; also the jejunal production of toll-like receptor-4 (TLR-4) was suppressed in the COS group. …”
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Altered Hepatic Lipid Metabolism Contributes to Nonalcoholic Fatty Liver Disease in Leptin-Deficient Ob/Ob Mice by James W. Perfield, Laura C. Ortinau, R. Taylor Pickering, Meghan L. Ruebel, Grace M. Meers, R. Scott Rector

“…Ob/Ob mice also had greater liver weights, hepatic triglyceride (TG) content, and markers of de novo lipogenesis, including increased hepatic gene expression and protein content of acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), and stearoyl-CoA desaturase-1 (SCD-1), as well as elevated gene expression of PPARγ and SREBP-1c compared with WT mice. While hepatic mRNA levels for PGC-1α, PPARα, and TFAM were elevated in Ob/Ob mice, measures of mitochondrial function (β-HAD activity and complete (to CO2) and total mitochondrial palmitate oxidation) and mitochondrial OXPHOS protein subunits I, III, and V content were significantly reduced compared with WT animals. …”
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Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway by Song Yang, Lei Yao, Xiaolong Wang, Hao Sun, Chaogang Du, Chengpeng Song, Jingyu Fu, Yongjun Wu, Hongwu Huang, Chuansi Wang, Yongsen Wang, Yixiang Xie

“…The underlying mechanisms were detected by Western blotting assays and BMP-2 si-RNA silencing assay in vitro and in vivo. Results. …”
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Human Bone Marrow Mesenchymal Stem Cells Promote Gastric Cancer Growth via Regulating c-Myc by Bin Chen, Jing Yu, Qianqian Wang, Yuanyuan Zhao, Li Sun, Changgen Xu, Xiangdong Zhao, Bo Shen, Mei Wang, Wenrong Xu, Wei Zhu

“…Moreover, we found that the c-Myc inhibitor JQ1 and c-Myc siRNA decreased the expression of c-Myc in hBM-MSC-CM-treated tumor cells in vitro. …”
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The Supernatant of Tonsil-Derived Mesenchymal Stem Cell Has Antiallergic Effects in Allergic Rhinitis Mouse Model by In-Su Park, Ji Hye Kim, Jun-Sang Bae, Dong-Kyu Kim, Ji-Hun Mo

“…Additionally, we found that the 10 mg T-MSCs-CM-treated group showed a significantly decreased IL-4 mRNA expression, compared to the (+) Con group. In the analysis of T cell receptor signal, the phosphorylation of MAP kinases, translocation of p65, and activation of NFAT1 were inhibited after T-MSCs-CM. …”
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Two Paediatric Patients with Encephalopathy and Concurrent COVID-19 Infection: Two Sides of the Same Coin? by Katerina Vraka, Dipak Ram, Siobhan West, Wei Yen Evelyn Chia, Praveen Kurup, Gayathri Subramanian, Hui Jeen Tan

“…Both patients had ADEM-like changes in their neuroimaging, negative SARS-CoV-2 RNA PCR in CSF, and paucity of PIMS-TS laboratory findings. …”
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Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways by Xiyao Pang, Ying Zhuang, Zehan Li, Shuanglin Jing, Qin Cai, Fengge Zhang, Changao Xue, Jinhua Yu

“…The expression of odonto/osteogenic markers was significantly upregulated in mRNA levels and protein levels. Moreover, intermittent treatment of PTH also increased phosphorylation of JNK and P38, and the differentiation was suppressed following the inhibition of JNK and P38 MAPK pathways. …”
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Preventive Effect of Liupao Tea Polyphenols on HCl/Ethanol-Induced Gastric Injury in Mice by Kai Zhu, Peng Peng, Ning Wu, Xianrong Zhou, Jianfei Mu, Xin Zhao

“…In addition, LTPs could upregulate the mRNA expression of Cu/Zn-SOD, Mn-SOD, CAT, nNOS, and eNOS and downregulate the expression of iNOS in the gastric tissue of mice with gastric injury. …”
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Decitabine Increases the Transcription of <i>RIG-I</i> Gene to Suppress the Replication of Feline Calicivirus and Canine Influenza Virus by Shaotang Ye, Zhen Wang, Aolei Chen, Ying Chen, Gaoming Lou, Qingmei Xie, Gang Lu, Shoujun Li

“…In summary, this study unveils the antiviral role of decitabine against FCV and CIV and provides evidence and novel insights into the demethylation drug-mediated antiviral effect for animal RNA viruses.…”
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A paper-based HPV E7 oncoprotein assay for cervical precancer detection at the point of care by Chelsey A. Smith, Sai Paul, Karen E. Haney, Sonia G. Parra, Meaghan Bond, Leticia López, Mauricio Maza, Juan Felix, Preetha Ramalingam, Pablo Escobar, Philip E. Castle, Kathleen M. Schmeler, Rebecca R. Richards-Kortum

“…Most commercially available tests are not appropriate for use in LMICs due to resource constraints. Specifically, HPV mRNA and oncoprotein tests that have high specificity for cervical precancer and cancer require complex sample preparation protocols and expensive instrumentation. …”
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Modulation of Preactivation of PPAR-β on Memory and Learning Dysfunction and Inflammatory Response in the Hippocampus in Rats Exposed to Global Cerebral Ischemia/Reperfusion by Ge Kuang, Qin He, Yunmei Zhang, Ruichun Zhuang, Anling Xiang, Qingsong Jiang, Ying Luo, Junqing Yang

“…For another, NF-κB p65 expression was significantly increased in hippocampal neurons with apparent nuclear translocation after global cerebral IRI, and these phenomena were also largely attenuated by GW0742 pretreatment. Moreover, the mRNA and protein expressions of PPAR-β were significantly decreased in GCIRI + GW0742 groups when compared with those in GCIRI group. …”
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Influence of Vinasse Application in the Structure and Composition of the Bacterial Community of the Soil under Sugarcane Cultivation by Wellington Pine Omori, André Ferreira de Camargo, Karla Cristina Stropa Goulart, Eliana Gertrudes de Macedo Lemos, Jackson Antônio Marcondes de Souza

“…The actual impacts of vinasse application on the selection of bacterial taxa are not understood because no studies have addressed this phenomenon directly. Analysis of 16S rRNA gene clone sequences from four soil types showed that the soil planted with sugarcane and fertilized with vinasse has a high diversity of bacteria compared to other biomes, where Acidobacteria were the second most abundant phylum. …”
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Siglec-E augments adipose tissue inflammation by modulating TRAF3 signaling and monocytic myeloid-derived suppressor cells during obesity by Ahmed Rakib, Mousumi Mandal, Md Abdullah Al Mamun, Sonia Kiran, Nelufar Yasmen, Lexiao Li, Daniel M. Collier, Jianxiong Jiang, Frank Park, Udai P. Singh

“…Immune cells, including macrophages, T cells, and neutrophils, infiltrate the AT and secrete proinflammatory cytokines to exacerbate the AT inflammation. RNA-Seq analysis of AT immune cells isolated from mice fed a high-fat diet (HFD) versus normal fat diet (ND) identified a panel of genes that were markedly downregulated, including sialic acid-binding Ig-like lectin E (siglec-E), in HFD compared to ND mice.MethodsA series of experiments in wild-type (WT) and siglec-E knockout (siglec-E KO) mice was designed to investigate the effect of HFD on the functional role of siglec-E in the regulation of AT inflammation and adipogenesis. …”
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Chronic Mild Stress Modulates Activity-Dependent Transcription of BDNF in Rat Hippocampal Slices by Raffaella Molteni, Andrea C. Rossetti, Elisa Savino, Giorgio Racagni, Francesca Calabrese

“…CMS reduces BDNF expression and that acute depolarization significantly upregulates total BDNF mRNA levels only in control animals, showing that CMS exposure may alter BDNF transcription under basal conditions and during neuronal activation. …”
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Uterine Fibroids: Pathogenesis and Interactions with Endometrium and Endomyometrial Junction by Andrea Ciavattini, Jacopo Di Giuseppe, Piergiorgio Stortoni, Nina Montik, Stefano R. Giannubilo, Pietro Litta, Md. Soriful Islam, Andrea L. Tranquilli, Fernando M. Reis, Pasquapina Ciarmela

“…To date, several pathogenetic factors such as genetics, microRNA, steroids, growth factors, cytokines, chemokines, and extracellular matrix components have been implicated in the development and growth of leiomyoma. …”
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Immunohistochemistry for (Pro)renin Receptor in Humans by Satoshi Morimoto, Noriko Morishima, Daisuke Watanabe, Yoichiro Kato, Noriyuki Shibata, Atsuhiro Ichihara

“…While (pro)renin receptor mRNA is widely expressed in various human tissues, localization of (pro)renin receptor protein expression has not yet been systemically determined. …”
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Tannic acid elicits differential gene regulation in prostate cancer apoptosis by Kandir Sinan, Karakurt Sevtap, Gökçek-Saraç Çiğdem, Karakurt Serdar

“…Cell proliferation, cytotoxicity, and effects of tannic acid on the cell death mechanism were evaluated. mRNA expression levels of 84 genes were explored in cells following tannic acid treatment. …”
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Comparative Study on Muscle Fiber Types of Longissimus Dorsi of Xinjiang Brown Cattle and Angus Cattle of Different Months by Ya-wei Sun, Miao Qu, Jiayinaguli Tuersunbai, Xuan-yi Wang, Tuolihanayi Tuoliken, Xiao-hong Zhang, Gang Yao, Huijun Shi

“…The morphology of the muscle was compared by ATP enzyme staining and SDH enzyme staining, and its gene expression was detected by qRT-PCR. The mRNA expression levels of Myhc-I in 3-month-old Xinjiang brown cattle were significantly higher than those in Angus cattle of the same age (P<0.01). …”
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