Optimization and characterization of bioactive secondary metabolites from Streptomyces sp CMSTAAHL-4 isolated from mangrove sediment by Selvaraj Jeraldin Nisha, Ganapathi Uma, Ramamoorthy Sathishkumar, Vincent Samuel Gnana Prakash, Rimal Isaac, Thavasimuthu Citarasu

“…The selected strain was identified as Streptomyces sp CMSTAAHL-4 by 16S rRNA sequencing. The media optimization for secondary metabolites production was performed by One-Variable at a Time and Response Surface Methodology-Central Composite Design. …”
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Impact of Endurance Exercise Training on Biomarkers of Aortic Endothelial Damage in Diabetic Rats by Mahtab Fouladi, Maryam Mahmoudabady, Zahra Gholamnezhad, Sadegh Shabab, Saeed Niazmand, Hossein Salmani

“…After 6 weeks, biochemical parameters in serum and oxidative stress markers and mRNA expression of endothelial nitric oxide synthase (eNOS), lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), and intercellular adhesion molecule-1 (ICAM-1) in aorta tissue were assessed. …”
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Regulation of Hypoxia-Induced Cell Death in Human Tenocytes by Min Liang, Hannah R. Cornell, Nasim Zargar Baboldashti, Mark S. Thompson, Andrew J. Carr, Philippa A. Hulley

“…Hypoxia markedly upregulated VEGF-A mRNA, followed by increased VEGF protein secretion. …”
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Application and Evaluation of [Tc]-Labeled Peptide Nucleic Acid Targeting in Breast Cancer Imaging by Yaqun Jiang MD, Yongkang Gai PhD, Yu Long MD, Qingyao Liu PhD, Chunbao Liu MD, PhD, Yongxue Zhang MD, Xiaoli Lan MD, PhD

“…It has been reported that dysregulation of microRNA-155 expression and function is associated with tumorigenesis, growth, tumor subtypes, invasion, and poor survival rates. …”
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CRYβB2 alters cell adhesion to promote invasion in a triple-negative breast cancer cell line by Amr A. Waly, London Harper, Jodie M. Fleming, Lindsey M. Costantini

“…A quantitative 3D invasion assay demonstrated that CRYβB2 overexpression significantly enhanced invasion (median invasion %; SUM159 = 0.14 and SUM159 + CRYβB2 = 0.33). RNA sequencing analysis indicated that CRYβB2 overexpression modulated cell-cell adhesion and extracellular matrix organization pathways, which are critical to invasion of cancer cells. …”
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Characterization of Microbiota in Endometrial Fluid and Vaginal Secretions in Infertile Women with Repeated Implantation Failure by Kotaro Kitaya, Yoko Nagai, Wataru Arai, Yoshiyuki Sakuraba, Tomomoto Ishikawa

“…Extracted genomic DNA was pyrosequenced for the V4 region of 16S ribosomal RNA using a next-generation sequencer. The EF microbiota had higher α-diversity and broader bacterial species than the VS microbiota both in the RIF and control groups. …”
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Potential biomarkers for predicting the risk of thyroid cancer in immunosenescence: a population-based and externally validated multi omics study by Qian Li, Yuanyuan Zhao, Jiawei Yan, Chao He

“…Finally, complementary results were obtained from an independent cohort analyzed at Chongqing Medical University Yongchuan Hospital and Bulk-RNA seq from GEO database.ResultsCausal analysis suggests that DNA methylation age acceleration as measured by the Hannum method increases the risk of thyroid cancer (OR: 1.126, 95% CI: 1.002-1.265, P=0.046). …”
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Emodin Ameliorates Intestinal Dysfunction by Maintaining Intestinal Barrier Integrity and Modulating the Microbiota in Septic Mice by Mina Zhang, Bo Lian, Rui Zhang, Yuhong Guo, Jingxia Zhao, Shasha He, Yunjing Bai, Ning Wang, Yan Lin, Xuerui Wang, Qingquan Liu, Xiaolong Xu

“…Bioinformatic analysis based on 16s rRNA gene sequencing illustrated that emodin treatment significantly increased the alpha- and beta-diversity of gut microbiota in septic mice. …”
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NEIL3 Mediates Lung Cancer Progression and Modulates PI3K/AKT/mTOR Signaling: A Potential Therapeutic Target by Hongbo Huang, Qingwang Hua

“…Quantitative real-time PCR (qRT-PCR) and western blot analyses were utilized to detect RNA and protein levels. Biological differences between groups were determined by gene set enrichment analysis (GSEA). …”
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FeLV-induced feline leukemia as a natural model for leukemia pathophysiology study (review) by T. V. Tabakaeva, A. V. Tsybulsky, K. A. Baranchugova, I. V. Galkina, M. Yu. Shchelkanov

“…Feline leukemia etiological agent is a feline leukemia virus (FeLV), having single-stranded RNA genomesurrounded with icosahedral capsidformedbyp27 capsid protein monomers. …”
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Liraglutide Promotes Osteoblastic Differentiation in MC3T3-E1 Cells by ERK5 Pathway by Yue Sun, Yuzhen Liang, Zhengming Li, Ning Xia

“…Moreover, the activity levels of alkaline phosphatase and the number of mineralized nodules in MC3T3-E1 cells were significantly increased following treatment with 100 nM liraglutide. The mRNA and protein levels of Col-1, OPG, and OCN in MC3T3-E1 cells were also markedly increased following 100 nM liraglutide treatment compared with those of the control group. …”
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Identifying Key Biomarkers Related to Immune Response in the Progression of Diabetic Kidney Disease: Mendelian Randomization Combined With Comprehensive Transcriptomics and Single-... by Hu M, Deng Y, Bai Y, Zhang J, Shen X, Shen L, Zhou L

“…Finally, we validated the mRNA expression of five key genes in the DKD mice model using reverse transcription quantitative PCR (RT-qPCR).Results: Five characteristic genes, known as protein kinase B beta (AKT2), interleukin-2 receptor beta (IL2RB), neurexin 3(NRXN3), slit homolog 3(SLIT3), and TATA box binding protein like protein 1 (TBPL1), demonstrated causal relationships with DKD. …”
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p104 Binds to Rac1 and Reduces Its Activity during Myotube Differentiation of C2C12 Cell by Ki Young Choi, Min Sup Lee, Young Jun Cho, Myong Ho Jeong, Seung Jin Han, Seung Hwan Hong

“…The overexpression of p104 enhanced myotube differentiation, whereas siRNA of p104 reversed this process. In this process, more Rac1 and CrkII were bound to increased p104. …”
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Reflections on COVID-19: A Literature Review of SARS-CoV-2 Testing by Chin Shern Lau, Helen M. L. Oh, Tar Choon Aw

“…Spike antibodies tracked the rise in antibodies after vaccination with mRNA vaccines and became invaluable in the assessment of vaccine response. …”
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Rice straw degradation by Pseudomonas aeruginosa AMB-CD-1, isolated from fresh cow dung and its impact on rice plants by Kishan Raj, Anup Singh, Tapas Chowdhury, S Gupta, Ravindra Soni

“…The isolated AMB-CD-1 was identified as Pseudomonas aeruginosa through the Sanger sequencing technique of the 16S rRNA gene. Results also revealed that the highest germination percentage, shoot weight and shoot length were found to be significantly superior in T4 (50% Sand + 50% Compost) whereas the highest chlorophyll content was found in T7 (25% Sand + 25% Soil + 50% Compost), and T1 (100% Sand) was effective in terms of root length.Conclusion Overall, the study concluded that Pseudomonas aeruginosa AMB-CD-1 might be a fast decomposer of paddy straw.…”
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Entamoeba histolytica: Gene Expression Analysis of Cells Invading Tissues by Helen C. Fernandes, Ana F. Costa, Michelle A. R. Freitas, Almir S. Martins, Jorge L. Pesquero, Élida M. Rabelo, Maria A. Gomes

“…One Brazilian strain of E. histolytica in two conditions, able or not to cause lesions in experimental animals, was used. RNA from this strain, was used to study the differential expression of genes. 29 specific gene fragments differentially expressed in the virulent strain were selected. …”
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Transmission of potato spindle tuber viroid between <i>Phytophthora infestans</i> and host plants by O. S. Afanasenko, A. V. Khiutti, N. V. Mironenko, N. M. Lashina

“…Potato spindle tuber viroid (PSTVd) is a naked, circular, single-stranded RNA (356–363 nucleotides in length) which lacks any protein-coding sequences. …”
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Pengaruh Pemberian Mesenchymal Stem Cells Wharton's Jelly terhadap Ekspresi Gen Sirtuin 1 pada Tikus Model Alzheimer by Havina Nurul Aizah, Amel Yanis, Hirowati Ali, Tofrizal Tofrizal, Cimi Ilmiawati, Dessy Arisanty

“…Methods: This was an experimental study with a post-test-only control group design, including three groups with a total sample of 18 samples of Alzheimer RNA rat models. Group-1without AlCl3 and MSC-WJ; Group-2 with AlCl3; Group-3 AlCl3 and MSC-WJ. …”
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ANKRD22 participates in the proinflammatory activities of macrophages in the colon cancer tumor microenvironment by Xiaoying Wang, Keqing Yang, Bin Yang, Rui Wang, Yongliang Zhu, Tianhui Pan

“…A significant decrease in ANKRD22 expression in macrophages cocultured with colon cancer cell culture supernatant as well as in macrophages directly derived from colorectal cancer tissues was observed. Single-cell RNA sequencing, spatial transcriptomic studies, and subcutaneous xenograft experiments in mice revealed that Ankrd22 silencing altered the subtype distribution of macrophages, attenuated their proinflammatory activity, and enhanced their protumor activity. …”
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Astragaloside IV inhibits pathological functions of gastric cancer-associated fibroblasts through regulation of the HOXA6/ZBTB12 axis by Liu Haibo, Luo Shicheng, Sha Xiaofeng, Chen Zhiping, Yang Dongdong

“…HOXA6 silencing also induced the downregulation of ZBTB12 mRNA and protein in CAFs. Astragaloside IV was demonstrated to regulate the expression of ZBTB12 by mediating the transcriptional activity of HOXA6. …”
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